Abstract:
BACKGROUND:Many biological systems respond to the presence or absence of gravity. Since experiments performed in space are expensive and can only be undertaken infrequently, Earth-based simulation techniques are used to investigate the biological response to weightlessness. A high gradient magnetic field can be used to levitate a biological organism so that its net weight is zero. RESULTS:We have used a superconducting magnet to assess the effect of diamagnetic levitation on the fruit fly D. melanogaster in levitation experiments that proceeded for up to 22 consecutive days. We have compared the results with those of similar experiments performed in another paradigm for microgravity simulation, the Random Positioning Machine (RPM). We observed a delay in the development of the fruit flies from embryo to adult. Microarray analysis indicated changes in overall gene expression of imagoes that developed from larvae under diamagnetic levitation, and also under simulated hypergravity conditions. Significant changes were observed in the expression of immune-, stress-, and temperature-response genes. For example, several heat shock proteins were affected. We also found that a strong magnetic field, of 16.5 Tesla, had a significant effect on the expression of these genes, independent of the effects associated with magnetically-induced levitation and hypergravity. CONCLUSIONS:Diamagnetic levitation can be used to simulate an altered effective gravity environment in which gene expression is tuned differentially in diverse Drosophila melanogaster populations including those of different age and gender. Exposure to the magnetic field per se induced similar, but weaker, changes in gene expression.
journal_name
BMC Genomicsjournal_title
BMC genomicsauthors
Herranz R,Larkin OJ,Dijkstra CE,Hill RJ,Anthony P,Davey MR,Eaves L,van Loon JJ,Medina FJ,Marco Rdoi
10.1186/1471-2164-13-52subject
Has Abstractpub_date
2012-02-01 00:00:00pages
52issn
1471-2164pii
1471-2164-13-52journal_volume
13pub_type
杂志文章相关文献
BMC GENOMICS文献大全abstract:BACKGROUND:The bacterial CRISPR system is fast becoming the most popular genetic and epigenetic engineering tool due to its universal applicability and adaptability. The desire to deploy CRISPR-based methods in a large variety of species and contexts has created an urgent need for the development of easy, time- and cos...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-016-3268-z
更新日期:2016-11-14 00:00:00
abstract:BACKGROUND:Decapods are an order of crustaceans which includes shrimps, crabs, lobsters and crayfish. They occur worldwide and are of great scientific interest as well as being of ecological and economic importance in fisheries and aquaculture. However, our knowledge of their biology mainly comes from the group which i...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-019-6433-3
更新日期:2020-01-09 00:00:00
abstract:BACKGROUND:As is true for many other antibiotic-resistant Gram-negative pathogens, members of the Burkholderia cepacia complex (BCC) are currently being assessed for their susceptibility to phage therapy as an antimicrobial treatment. The objective of this study was to perform genomic and limited functional characteriz...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-14-574
更新日期:2013-08-27 00:00:00
abstract:BACKGROUND:Next-Generation Sequencing has revolutionized our approach to ancient DNA (aDNA) research, by providing complete genomic sequences of ancient individuals and extinct species. However, the recovery of genetic material from long-dead organisms is still complicated by a number of issues, including post-mortem D...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-13-178
更新日期:2012-05-10 00:00:00
abstract:BACKGROUND:High throughput experiments resulted in many genomic datasets and hundreds of candidate disease genes. To discover the real disease genes from a set of candidate genes, computational methods have been proposed and worked on various types of genomic data sources. As a single source of genomic data is prone of...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-13-S7-S27
更新日期:2012-01-01 00:00:00
abstract:BACKGROUND:The incidence of congenital heart disease (CHD) is continuously increasing among infants born alive nowadays, making it one of the leading causes of infant morbidity worldwide. Various studies suggest that both genetic and environmental factors lead to CHD, and therefore identifying its candidate genes and d...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-12-592
更新日期:2011-12-02 00:00:00
abstract::Following the publication of this article [1], the authors reported that the original shading in columns 3 and 4 of Table 3, which indicated the presence or absence of viruses in each library, had been removed during typesetting. ...
journal_title:BMC genomics
pub_type: 杂志文章,已发布勘误
doi:10.1186/s12864-019-6067-5
更新日期:2019-09-05 00:00:00
abstract:BACKGROUND:Gene expression profiling is a promising approach to better estimate patient prognosis; however, there are still unresolved problems, including little overlap among similarly developed gene sets and poor performance of a developed gene set in other datasets. RESULTS:We applied a gene sets approach to develo...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-9-177
更新日期:2008-04-16 00:00:00
abstract:BACKGROUND:Closely related species of the carp family (Cyprinidae) have evolved distinctive abilities to survive under cold stress, but molecular mechanisms underlying the generation of cold resistance remain largely unknown. In this study, we compared transcriptomic profiles of two carp species to identify key factors...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-020-06946-8
更新日期:2020-08-05 00:00:00
abstract:BACKGROUND:Gonadotropin releasing hormone (GnRH) is responsible for stimulation of gonadotropic hormone (GtH) in the hypothalamus-pituitary-gonadal axis (HPG). The regulatory mechanisms responsible for brain specificity make the promoter attractive for in silico analysis and reporter gene studies in zebrafish (Danio re...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-3-25
更新日期:2002-08-21 00:00:00
abstract:BACKGROUND:The globe artichoke (Cynara cardunculus var. scolymus L.) is a significant crop in the Mediterranean basin. Despite its commercial importance and its both dietary and pharmaceutical value, knowledge of its genetics and genomics remains scant. Microsatellite markers have become a key tool in genetic and genom...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-10-454
更新日期:2009-09-28 00:00:00
abstract:BACKGROUND:Cultivated watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai var. lanatus] is an important agriculture crop world-wide. The fruit of watermelon undergoes distinct stages of development with dramatic changes in its size, color, sweetness, texture and aroma. In order to better understand the genetic and m...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-12-454
更新日期:2011-09-21 00:00:00
abstract:BACKGROUND:The ciliate Paramecium bursaria harbors several hundred cells of the green-alga Chlorella sp. in their cytoplasm. Irrespective of the mutual relation between P. bursaria and the symbiotic algae, both cells retain the ability to grow without the partner. They can easily reestablish endosymbiosis when put in c...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-15-183
更新日期:2014-03-10 00:00:00
abstract:BACKGROUND:Gibel carp is an important aquaculture species in China, and a herpesvirus, called as Carassius auratus herpesvirus (CaHV), has hampered the aquaculture development. Diverse gynogenetic clones of gibel carp have been identified or created, and some of them have been used as aquaculture varieties, but their r...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-017-3945-6
更新日期:2017-07-24 00:00:00
abstract:BACKGROUND:Fistular leaves frequently appear in Allium species, and previous developmental studies have proposed that the process of fistular leaf formation involves programmed cell death. However, molecular evidence for the role of programmed cell death in the formation of fistular leaf cavities has yet to be reported...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-016-3474-8
更新日期:2017-01-10 00:00:00
abstract:BACKGROUND:Plant-bacteria associations have been extensively studied for their potential in increasing crop productivity in a sustainable manner. Serratia marcescens is a species of Enterobacteriaceae found in a wide range of environments, including soil. RESULTS:Here we describe the genome sequencing and assessment o...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-018-5130-y
更新日期:2018-10-16 00:00:00
abstract:BACKGROUND:Previously, we could show that L-lactate affects cultured bovine granulosa cells (GC) in a specific manner driving the cells into an early pre-ovulatory phenotype. Here we studied genome wide effects in L-lactate-treated GC to further elucidate the underlying mechanisms that are responsible for the L-lactate...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-019-5657-6
更新日期:2019-04-05 00:00:00
abstract:BACKGROUND:Human leukocyte antigen (HLA)-B27 is strongly associated with the development of reactive arthritis (ReA) in humans after salmonellosis. Human monocytic U937 cells transfected with HLA-B27 are less able to eliminate intracellular Salmonella enterica serovar Enteritidis than those transfected with control HLA...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-11-456
更新日期:2010-07-30 00:00:00
abstract:BACKGROUND:In a previous genome-wide analysis of FXR binding to hepatic chromatin, we noticed that an extra nuclear receptor (NR) half-site was co-enriched close to the FXR binding IR-1 elements and we provided limited support that the monomeric LRH-1 receptor that binds to NR half-sites might function together with FX...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-13-51
更新日期:2012-02-01 00:00:00
abstract:BACKGROUND:Oxidative stress is a consequence of normal and abnormal cellular metabolism and is linked to the development of human diseases. The effective functioning of the pathway responding to oxidative stress protects the cellular DNA against oxidative damage; conversely the failure of the oxidative stress response ...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-13-S6-S4
更新日期:2012-01-01 00:00:00
abstract:BACKGROUND:Alternative splicing (AS) is an important regulatory mechanism that greatly contributes to eukaryotic transcriptome diversity. A substantial amount of evidence has demonstrated that AS complexity is relevant to eukaryotic evolution, development, adaptation, and complexity. In this study, six teosinte and ten...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-015-1582-5
更新日期:2015-05-08 00:00:00
abstract:BACKGROUND:Hepatitis C virus (HCV) is a rapidly evolving RNA virus that has been classified into seven genotypes. All HCV genotypes cause chronic hepatitis, which ultimately leads to liver diseases such as cirrhosis. The genotypes are unevenly distributed across the globe, with genotypes 1 and 3 being the most prevalen...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-016-2575-8
更新日期:2016-03-17 00:00:00
abstract:BACKGROUND:MicroRNAs (miRNAs) are small non-coding RNAs that play important roles by regulating other genes. Rosa rugosa Thunb. is an important ornamental and edible plant, yet there are only a few studies on the miRNAs and their functions in R. rugosa. RESULTS:We sequenced 10 samll RNA profiles from the roots, petals...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-018-5325-2
更新日期:2019-04-18 00:00:00
abstract:BACKGROUND:Obesity affects quality of life and life expectancy and is associated with cardiovascular disorders, cancer, diabetes, reproductive disorders in women, prostate diseases in men, and congenital anomalies in children. The use of single nucleotide polymorphism (SNP) markers of diseases and drug responses (i.e.,...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-16-S13-S5
更新日期:2015-01-01 00:00:00
abstract:BACKGROUND:Next generation sequencing (NGS) has become a universal practice in modern molecular biology. As the throughput of sequencing experiments increases, the preparation of conventional multiplexed libraries becomes more labor intensive. Conventional library preparation typically requires quality control (QC) tes...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-020-6481-8
更新日期:2020-01-28 00:00:00
abstract:BACKGROUND:Carolacton is a newly identified secondary metabolite causing altered cell morphology and death of Streptococcus mutans biofilm cells. To unravel key regulators mediating these effects, the transcriptional regulatory response network of S. mutans biofilms upon carolacton treatment was constructed and analyze...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-15-362
更新日期:2014-05-12 00:00:00
abstract:BACKGROUND:Human interfollicular epidermis is sustained by the proliferation of stem cells and their progeny, transient amplifying cells. Molecular characterization of these two cell populations is essential for better understanding of self renewal, differentiation and mechanisms of skin pathogenesis. The purpose of th...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-9-359
更新日期:2008-07-30 00:00:00
abstract:BACKGROUND:Malaria, caused by the parasitic protist Plasmodium falciparum, represents a major public health problem in the developing world. The P. falciparum genome has been sequenced, which provides new opportunities for the identification of novel drug targets. Eukaryotic protein kinases (ePKs) form a large family o...
journal_title:BMC genomics
pub_type: 杂志文章,评审
doi:10.1186/1471-2164-5-79
更新日期:2004-10-12 00:00:00
abstract:BACKGROUND:The obligate intracellular parasite Toxoplasma gondii establishes a life-long chronic infection within any warm-blooded host. After ingestion of an encysted parasite, T. gondii disseminates throughout the body as a rapidly replicating form during acute infection. Over time and after stimulation of the host i...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-15-806
更新日期:2014-09-20 00:00:00
abstract:BACKGROUND:RNA-Seq is now widely used as a research tool. Choices must be made whether to use paired-end (PE) or single-end (SE) sequencing, and whether to use strand-specific or non-specific (NS) library preparation kits. To date there has been no analysis of the effect of these choices on identifying differentially e...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-017-3797-0
更新日期:2017-05-23 00:00:00