Two CYP3A-like genes in the marine mussel Mytilus edulis: mRNA expression modulation following short-term exposure to endocrine disruptors.

Abstract:

:Members of the vertebrate CYP3A subfamily are involved in the metabolism of steroids and a wide range of xenobiotics. In this study two CYP3A-like mRNAs have been isolated from the mussel (Mytilus edulis), and their seasonal expression profile and modulation by estrogens examined. Sexual dimorphism of CYP3A-like mRNA expression was not observed in mussel gonads of individuals collected throughout a year. Nevertheless, natural variation in gonadal CYP3A-like mRNA expression was observed, with highest levels of CYP3A isoform1 and lowest levels of CYP3A isoform2 mRNA during the maturation and spawning season. Exposure to a 10% sewage treatment works extract did not result in any significant changes in mRNA expression of CYP3A-like. In contrast, exposure to E2 (200 ng/L) and TBT (100 ng/L) significantly down-regulated the expression of CYP3A-like isoform1 but not CYP3A-like isoform2 suggesting differential regulation.

journal_name

Mar Environ Res

authors

Cubero-Leon E,Puinean AM,Labadie P,Ciocan C,Itoh N,Kishida M,Osada M,Minier C,Hill EM,Rotchell JM

doi

10.1016/j.marenvres.2011.11.012

subject

Has Abstract

pub_date

2012-03-01 00:00:00

pages

32-9

eissn

0141-1136

issn

1879-0291

pii

S0141-1136(11)00164-4

journal_volume

74

pub_type

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