Abstract:
:Gene therapy holds great potential for treating neurological disorders. However, delivering gene vectors to the brain has been either invasive or inefficacious in most studies to date. The aim of this study was to develop a safe and efficacious strategy for delivering gene vectors to the brain. A tetracycline-inducible replication-defective herpes simplex virus type-1 vector, QR9TO-LacZ, was administered to rats intranasally. QR9TO-LacZ could infect primary cortical neurons and express the reporter gene without detectable replication. QR9TO-LacZ was observed in the olfactory bulb, hippocampus, striatum, cortex, medulla, cerebellum, ventricles, and nasal septum after intranasal administration. Expression of the reporter gene could be controlled effectively by tetracycline. In vitro, introduction of QR9TO-LacZ did not change the structure of transfected neurons. In vivo, QR9TO-LacZ did not increase apoptosis in neurons and did not alter levels of interleukin 6 and tumor necrosis factor α in the brain after intranasal delivery. Our data suggest that intranasally applied QR9TO-LacZ has a wide distribution and expresses the reporter gene in the brain under the control of tetracycline with less cytotoxicity than intravenous or stereotactic delivery methods.
journal_name
Neurosciencejournal_title
Neuroscienceauthors
Jiang Y,Wei N,Zhu J,Zhai D,Wu L,Chen M,Xu G,Liu Xdoi
10.1016/j.neuroscience.2011.10.043subject
Has Abstractpub_date
2012-01-10 00:00:00pages
96-104eissn
0306-4522issn
1873-7544pii
S0306-4522(11)01236-Xjournal_volume
201pub_type
杂志文章相关文献
NEUROSCIENCE文献大全abstract::There is increasing evidence that alterations in the focus of attention result in changes in neural responding at the most peripheral levels of the auditory system. To date, however, those studies have not ruled out differences in task demands or overall arousal in explaining differences in responding across intermoda...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2012.07.062
更新日期:2012-10-25 00:00:00
abstract::Long-term hyperexcitability was found after unilateral, permanent middle cerebral artery occlusion in exofocal neocortical areas of the adult mouse [Mittmann et al. (1998) Neuroscience 85, 15-27]. The aim of the present study was to test the hypothesis in an identical paradigm of ischemia. whether alterations in the d...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(97)00656-8
更新日期:1998-07-01 00:00:00
abstract::We investigated the electrophysiology of morphologically identified human granule cells with conventional current-clamp recordings. Slices were prepared from 14 human epileptic sclerotic hippocampi. Granule cells appeared to have a diverse electrophysiology. Each cell was distinguished by the shape of the afterhyperpo...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(98)00574-0
更新日期:1999-01-01 00:00:00
abstract::Eleven healthy tobacco smoking adult male volunteers of mixed race were tobacco abstinent overnight for this study. In each subject, positron emission tomographic images of regional cerebral metabolism of glucose with [18F]fluorodeoxyglucose were obtained in two conditions in the morning on different days: about 3min ...
journal_title:Neuroscience
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/s0306-4522(00)00357-2
更新日期:2000-01-01 00:00:00
abstract::Acetylcholine can stimulate the release of vasopressin. In organ-cultured hypothalamo-neurohypophyseal systems, acetylcholine enhanced vasopressin release by acting in or near the supraoptic nucleus Extracellular recordings suggested that acetylcholine can increase supraoptic neuron excitability. These effects could b...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(99)00477-7
更新日期:2000-01-01 00:00:00
abstract::The course of the ascending pathways originating from the anterior gustatory and posterior visceral sensory part of the solitary tract nucleus and the topographic organization of the projections to the hypothalamus in the rat were studied with anterogradely transported Phaseolus vulgaris lectin. In general, the poster...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(89)90441-7
更新日期:1989-01-01 00:00:00
abstract::Brain-derived neurotrophic factor (BDNF) is a neurotrophin (NT) known to participate in chronic somatic pain. A recent study has indicated that BDNF may participate in chronic cystitis at the peripheral level. However, the principal site of action for this NT is the central nervous system, most notably the spinal cord...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2012.12.044
更新日期:2013-03-27 00:00:00
abstract::The Fmr1 gene knockout mouse is a model for the human Fragile X mental retardation syndrome. Fmr1 knockout mice with a C57BL/6-129/OlaHsd hybrid background have been reported to have only a very mild deficiency in learning the Morris water maze task. We compared the effect of this knockout mutation on learning in mice...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(00)00292-x
更新日期:2000-01-01 00:00:00
abstract::A salient feature of the developing brain is that spontaneous oscillations (SOs) and waves may influence the emergence of synaptic connections. While GABA produces depolarization and may support SOs in the neurons of developing rodents, it elicits hyperpolarization and diminishes SOs in developing gerbil auditory cort...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2012.09.008
更新日期:2012-12-13 00:00:00
abstract::The effects of L-glutamate on the potency of kainate for stimulating guanosine 3',5'-cyclic monophosphate (cyclic GMP) accumulation and for killing neurones in incubated slices of immature (8-day) and adult rat cerebellum were investigated. L-glutamate did not potentiate the cyclic GMP responses to kainate in either t...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(84)90218-5
更新日期:1984-01-01 00:00:00
abstract::Using structural magnetic resonance imaging in a clinical scanner at 3.0T, we describe results showing that following 12weeks on a diet of 2% cholesterol, rabbits experience a significant increase in the volume of the third ventricle compared to rabbits on a diet of 0% cholesterol. Using time-of-flight magnetic resona...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2013.09.015
更新日期:2013-12-19 00:00:00
abstract::The capacity of neural stem cells (NSC) to generate different types of neurons and glia depends on the action of intrinsic determinants and extracellular signals. Here, we isolated adult olfactory bulb stem cells (aOBSC) that express nestin, RC2 and Sox2, and that have the capacity to generate neurons possessing matur...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2009.03.033
更新日期:2009-08-04 00:00:00
abstract::It has been observed that motor neuron death is induced in adult rats by ventral root avulsion which involves pulling out the spinal cord root. Since motor neurons are reported to be selectively vulnerable to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate receptor-mediated injury in vitro, we investigated chang...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(02)00816-3
更新日期:2003-01-01 00:00:00
abstract::Previous studies suggested that Group I metabotropic glutamate (mGlu) receptors play a role in mechanotransduction processes of slowly adapting type I mechanoreceptors. Using an isolated rat sinus hair follicle preparation we tested a range of compounds. Surprisingly, only non-competitive mGlu1 receptor antagonists pr...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2009.07.015
更新日期:2009-10-20 00:00:00
abstract::Our study examined how different housing conditions modulated the acquisition of a spatial reference memory task and also, a reversal task in the 4-radial arm water maze (4-RAWM). The animals were randomly assigned to standard or enriched cages, and, as a type of complementary stimulation along with the environmental ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2015.08.038
更新日期:2015-10-29 00:00:00
abstract::Brain structural connectivity is known to be altered in cases of intrauterine growth restriction and premature birth, although the specific effect of maternal nutritional restriction, a common burden in human populations, has not been assessed yet. Here we analyze the effects of maternal undernutrition during pregnanc...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2018.03.049
更新日期:2018-06-01 00:00:00
abstract::Endotoxin-induced uveitis (EIU) is a common animal model for anterior uveitis in humans that causes long-term changes in trigeminal brain stem neurons. This study used c-fos immunohistochemistry to assess the effects of different routes of administration of endotoxin on activation of trigeminal brain stem neurons prod...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2008.12.015
更新日期:2009-03-17 00:00:00
abstract::In order to observe the fine details of intracellularly stained neurons in brain slices the slices must be cleared of opaque matter. This clearing process involves dehydration of the slice, which typically results in significant shrinkage of the cleared tissue. However, how this shrinkage affects neuronal morphology h...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(85)90018-1
更新日期:1985-10-01 00:00:00
abstract::The intracochlear infusion of neurotrophic factors via a mini-osmotic pump has been shown to prevent deafness-induced spiral ganglion neuron (SGN) degeneration; however, the use of pumps may increase the incidence of infection within the cochlea, making this technique unsuitable for neurotrophin administration in a cl...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2007.11.057
更新日期:2008-03-27 00:00:00
abstract::Recently, we reported that erythropoietin attenuates neonatal brain injury caused by focal cerebral ischemia. The long-term effects of erythropoietin on focal cerebral ischemia-induced injury to the developing brain and the potential gender differences in these long-term effects have not been studied in detail. The cu...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2006.02.057
更新日期:2006-01-01 00:00:00
abstract::The auditory system continuously monitors the environment for irregularities in an automatic, preattentive fashion. This is presumably accomplished by two mechanisms: a sensory mechanism detects a deviant sound on the basis of differential refractoriness of neural populations sensitive to the standard and deviant soun...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2013.08.039
更新日期:2013-12-03 00:00:00
abstract::The possible roles of G-protein coupled metabotropic glutamate receptors in central nervous function are currently the focus of intensive investigation. The complexity of effects produced by agonists at these receptors probably reflects the activity of a range of sub-types. The metabotropic glutamate receptors first d...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(93)90400-a
更新日期:1993-02-01 00:00:00
abstract::Transient global ischemia results in delayed selective neuronal death of hippocampal CA1 pyramidal cells. Glucocorticoids increase and adrenalectomy decreases the rate of neuronal death; however, they also produce changes in brain temperature, serum glucose and adrenocorticotropic hormone levels. In order to understan...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(98)00213-9
更新日期:1999-01-01 00:00:00
abstract::Immunohistochemical methods were used to characterize the distribution of staining for leucine enkephalin-like and methionine enkephalin-like immunoreactivities in the telencephalon of Caiman crocodilus. Very similar distributions of both leucine enkephalin-like and methionine enkephalin-like immunoreactivity were obs...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(84)90028-9
更新日期:1984-02-01 00:00:00
abstract::The distribution of CA3 hippocampal axonal terminal fields undergo a period of widespread connectivity-based changes in the early postnatal stages of life. The purpose of the current study was to examine the effect of acute phthalate exposure during this period of hippocampal development (postnatal days 16-22 (p16-p22...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2011.06.082
更新日期:2011-10-13 00:00:00
abstract::The mesopontine tegmentum, which contains both cholinergic and non-cholinergic neurons, plays a crucial role in behavioral state control. Using microiontophoresis in unanesthetized cats, we have examined the effect of cholinergic and monoaminergic drugs on two putative cholinergic neurons located mostly in the laterod...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(00)00004-x
更新日期:2000-01-01 00:00:00
abstract::In anaesthetized rats, the intraspinal release of immunoreactive prostaglandin E2 was measured using antibody microprobes. We addressed the question of whether the release of immunoreactive prostaglandin E2 is altered during development of acute inflammation in the knee evoked by intra-articular injections of kaolin a...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(99)00164-5
更新日期:1999-01-01 00:00:00
abstract::Previous studies have shown the feasibility of using diffusion tensor imaging (DTI) as a noninvasive imaging modality to evaluate neurodegeneration in humans and animals. The axial and radial diffusivities derived from DTI were demonstrated to be sensitive markers for axonal and myelin damage, respectively. This study...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2011.09.042
更新日期:2011-12-01 00:00:00
abstract::Clozapine, an atypical antipsychotic agent important for the treatment of schizophrenia, has marked inhibitory effects on sympathetic outflow to the thermoregulatory cutaneous circulation. In rabbits clozapine reverses ear pinna vasoconstriction induced either by administration of MDMA (3,4-methylenedioxymethamphetami...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2006.05.050
更新日期:2006-09-15 00:00:00
abstract::Zinc plays an important role in synaptic signaling in the mammalian cerebral cortex. Zinc is sequestered into presynaptic vesicles of subpopulations of glutamatergic neurons and is released by depolarization, in a calcium-dependent manner. As the majority of mechanisms that have been suggested to participate in experi...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(03)00292-6
更新日期:2003-01-01 00:00:00