Abstract:
INTRODUCTION:Bispecific monoclonal antibodies (bsMAbs) have been developed as a pretargeting tool to reduce background activity, thereby increasing target to background (T : B) ratios. To enhance visualization of small lesions in vivo, we have used the pretargeting approach of bsMAb and negatively charged polymers radiolabeled with high-specific radioactivity. METHODS:Imaging of metastatic melanoma lesions localized in lung tissues pretargeted with bsMAb and targeted with high-specific radioactivity polymers was carried out. The bsMAb was prepared by covalent conjugation of an antinucleosomal antibody (2C5) recognizing a nucleosomal pan cancer antigen and an anti-diethylene triaminepentaacetic acid antibody (6C31H3) by means of thioether linkage. BsMAb was injected intravenously 10 days after the initiation of the induction of murine melanoma metastasized to the lungs. The next day, 37 MBq ⁹⁹mTc-diethylene triaminepentaacetic acid-succinylated polylysine were injected intravenously and in-vivo imaging was carried out after the injection. In-vivo and ex-vivo target (T) to background (B) activity ratios were assessed by computer planimetry and biodistribution studies. RESULTS:Lesions were visualized unequivocally in 3 h by gamma scintigraphy. Ex-vivo gamma-scintillation counting corrected for the lesion mass showed that the mean lesion activity was 24.85 ± 13.53 percent injected dose per gram when pretargeted with bsMAb, whereas it was 0.977 ± 0.465 percent injected dose per gram (P<0.01) in the control group injected only with radioactive polymers also corrected similarly. CONCLUSION:The use of bsMAb complexes and ⁹⁹mTc-diethylene triaminepentaacetic acid-succinylated polylysine enabled early in-vivo visualization of small metastatic melanoma lesions in the lungs.
journal_name
Nucl Med Communjournal_title
Nuclear medicine communicationsauthors
Gada KS,Patil V,Panwar R,Majewski S,Tekabe Y,Khaw BAdoi
10.1097/MNM.0b013e32834af77bsubject
Has Abstractpub_date
2011-12-01 00:00:00pages
1231-40issue
12eissn
0143-3636issn
1473-5628journal_volume
32pub_type
杂志文章abstract::This paper proposes the utilization of 225Ac for the alpha-radioimmunotherapy of cancer. The isotope decays with a radioactive half-life of 10 days into a cascade of short-lived alpha- and beta-emitting isotopes. In addition, when indicated by the pharmacokinetic requirements of particular clinical applications, 213Bi...
journal_title:Nuclear medicine communications
pub_type: 杂志文章
doi:10.1097/00006231-199302000-00009
更新日期:1993-02-01 00:00:00
abstract::This work was designed to compare sentinel lymph node (SLN) uptake of 99mTc-labelled human serum albumin colloid (99mTc-HSAC), 99mTc-labelled antimony sulphur colloid (99mTc-SC) and a 99mTc-labelled dextran 70 solution (99mTc-Dx) and their selectivity in the identification of this node in the right rear footpad (RRF) ...
journal_title:Nuclear medicine communications
pub_type: 杂志文章
doi:10.1097/00006231-200105000-00006
更新日期:2001-05-01 00:00:00
abstract::This study surveyed practice in nuclear cardiology in the UK in 1994. A questionnaire was sent to 219 centres performing nuclear imaging asking for details of current practice in nuclear cardiology. Replies were received from 192 centres (88%). Activity in performance of myocardial perfusion imaging (MPI) and radionuc...
journal_title:Nuclear medicine communications
pub_type: 杂志文章
doi:10.1097/00006231-199804000-00004
更新日期:1998-04-01 00:00:00
abstract::Radioimmunoscintigraphy (RIS) and radioimmunoguided surgery (RIGS) were assessed for their usefulness in patients with colorectal carcinoma. Twenty-nine patients (18 primary tumours, 10 with a suspicion of recurrence and one colonic diverticulitis) were studied. Radioimmunoscintigraphy was performed 48 and 72 h after ...
journal_title:Nuclear medicine communications
pub_type: 杂志文章
doi:10.1097/00006231-199902000-00003
更新日期:1999-02-01 00:00:00
abstract::Fever of unknown origin is defined as a temperature above 39.0 degrees C together with a white blood cell count > or = 15,000 mm-3, the duration of fever exceeding 2 weeks and a correct diagnosis not being obtained in the first week of hospitalization. In neonates and infants with fever of unknown origin, the localiza...
journal_title:Nuclear medicine communications
pub_type: 杂志文章
doi:10.1097/00006231-199811000-00003
更新日期:1998-11-01 00:00:00
abstract::When preparing 99Tcm-exametazime, it is important to use sodium 99Tcm-pertechnetate that is less than 2 h old. The addition of sodium iodide (NaI) to 99Tcm-pertechnetate is known to extend this time to 6 h. This paper describes a technique for implementing this in routine practice. Sterile kits consisting of 440 micro...
journal_title:Nuclear medicine communications
pub_type: 杂志文章
doi:10.1097/00006231-199205000-00003
更新日期:1992-05-01 00:00:00
abstract::Captopril renography is used for the non-invasive diagnosis of renovascular hypertension, but suffers from the drawbacks of lower sensitivity and false-positive tests due to a fall in blood pressure. Aspirin renography has been proposed as a useful test for evaluation of unilateral renal artery stenoses of moderate de...
journal_title:Nuclear medicine communications
pub_type: 杂志文章
doi:10.1097/00006231-200004000-00006
更新日期:2000-04-01 00:00:00
abstract::Heart uptake and clearance of 123I-15-(p-iodophenyl)-3(R,S)-methyl- pentadecanoic acid (BMIPP) by whole-body scintigraphy in relation to left ventricular ejection fraction (LVEF) was evaluated in 34 patients with ischaemic heart disease. At 45 and 210 min after injection, anterior whole-body images were obtained. In e...
journal_title:Nuclear medicine communications
pub_type: 临床试验,杂志文章
doi:10.1097/00006231-199409000-00004
更新日期:1994-09-01 00:00:00
abstract::The radiation dose to the pulp of both index fingers has been measured in a radiopharmacy supplying 11 000 patient doses a year, in a hospital dispensary (4500 doses a year) and in its injection area. Tungsten syringe shields were used for one week and not used during the other week. In the radiopharmacy and the dispe...
journal_title:Nuclear medicine communications
pub_type: 杂志文章
doi:10.1097/00006231-198508000-00004
更新日期:1985-08-01 00:00:00
abstract:OBJECTIVE:The liver is the most frequent metastatic site from colorectal cancer and about 20% of these patients are treated by surgical resection. However, the 5-year disease-free survival (DFS) following resection is only about 25% and 5-year overall survival (OS) about 38%. The aim of the study was to evaluate the ab...
journal_title:Nuclear medicine communications
pub_type: 杂志文章
doi:10.1097/MNM.0000000000001235
更新日期:2020-09-01 00:00:00
abstract::Salivary gland scintigraphy (SGS) is used to depict salivary gland dysfunction after radiotherapy (RT). The aim of this study was to investigate the utility of SGS combined with single photon emission computed tomography (SPECT). Twenty-one patients with a carcinoma of head and neck underwent SGS before and 1 month af...
journal_title:Nuclear medicine communications
pub_type: 临床试验,杂志文章
doi:10.1097/00006231-200102000-00015
更新日期:2001-02-01 00:00:00
abstract:OBJECTIVE:The aim of the study was to evaluate gated myocardial perfusion SPECT (GMPS) in the prediction of response to cardiac resynchronization therapy (CRT) in nonischaemic dilated cardiomyopathy patients. PATIENTS AND METHODS:Thirty-two patients (23 men, mean age 57.5±12.1 years) with severe heart failure, who wer...
journal_title:Nuclear medicine communications
pub_type: 临床试验,杂志文章
doi:10.1097/MNM.0000000000000282
更新日期:2015-05-01 00:00:00
abstract::An examination of the biokinetics and biodistribution of i.p. administered 131I- or 111In-labelled 791T/36 antibody (1 mg) has been carried out in five patients with stage III/IV ovarian cancer. Blood kinetics and urinary excretion of the radiolabels were assayed. Scintigraphy was performed immediately following admin...
journal_title:Nuclear medicine communications
pub_type: 杂志文章
doi:10.1097/00006231-198908010-00006
更新日期:1989-08-01 00:00:00
abstract:PURPOSE:The aim of this study was to evaluate the clinical role of technetium-99m pertechnetate (Tc-99m) imaging in thyroidectomized differentiated thyroid cancer patients immediately before radioiodine-131 (I-131) treatment (Tx). PATIENT AND METHODS:Eighty-six consecutive post-total-thyroidectomy patients (15 men, 71...
journal_title:Nuclear medicine communications
pub_type: 杂志文章
doi:10.1097/MNM.0000000000000426
更新日期:2016-02-01 00:00:00
abstract::Should one correct for extrathyroidal neck radioactivity ('neck background') when interpreting perchlorate discharge test results? A new background correction method was developed using a linear probe. 123I produces photon peaks at two energy levels: 159 keV (gamma-rays) and 28 keV (X-rays), with an attenuation of 15 ...
journal_title:Nuclear medicine communications
pub_type: 杂志文章
doi:10.1097/00006231-199211000-00013
更新日期:1992-11-01 00:00:00
abstract:OBJECTIVE:The staging and management of patients with newly diagnosed nonsmall cell lung cancer (NSCLC) in the setting of recently diagnosed other (metachronous or synchronous) primary cancer are challenging. This retrospective cohort study was carried out to test our hypothesis that baseline 2-deoxy-2-[F]fluoro-D-gluc...
journal_title:Nuclear medicine communications
pub_type: 杂志文章
doi:10.1097/MNM.0000000000000627
更新日期:2017-02-01 00:00:00
abstract::Foot problems are common causes of disability in diabetic patients with as many as 25% expected to develop severe foot or leg problems during their lifetimes. Although skin ulceration is the most frequent problem, bones may also be involved in two different clinical conditions: osteomyelitis and Charcot osteoarthropat...
journal_title:Nuclear medicine communications
pub_type: 杂志文章,评审
doi:10.1097/01.mnm.0000230066.23823.cc
更新日期:2006-09-01 00:00:00
abstract:OBJECTIVE:To measure and describe patterns of interobserver variation in visual interpretation of 18-FDG PET in malignant lymphoma. METHODS:Eleven nuclear medicine physicians with different levels of PET experience independently reviewed 37 18F-FDG PET scans of lymphoma patients (10 obtained at presentation, 27 during...
journal_title:Nuclear medicine communications
pub_type: 杂志文章
doi:10.1097/MNM.0b013e3282eff2d5
更新日期:2007-10-01 00:00:00
abstract:PURPOSE:Respiratory gated perfusion single photon emission computed tomography (SPECT) was applied to reduce respiratory lung motion effects and to reliably assess perfusion impairment in various lung diseases. METHODS:After injection of 259 MBq of 99mTc macroaggretated albumin (99mTc-MAA), gating was performed using ...
journal_title:Nuclear medicine communications
pub_type: 临床试验,杂志文章
doi:10.1097/00006231-200402000-00016
更新日期:2004-02-01 00:00:00
abstract:AIM:In patients with differentiated thyroid carcinoma (DTC), metastases can either show iodine-131 (I) uptake on whole-body scintigraphy or F-2-fluoro-2-deoxy-D-glucose (F-FDG) uptake on combined PET and X-ray computed tomography (PET/CT), or a mix of both. The present study investigates the relationship between uptake...
journal_title:Nuclear medicine communications
pub_type: 杂志文章
doi:10.1097/MNM.0000000000000025
更新日期:2014-02-01 00:00:00
abstract:OBJECTIVES:The determination of lesion boundaries on FDG PET is difficult due to the point-spread blurring and unknown uptake of activity within a lesion. Standard threshold-based methods for volumetric quantification on PET usually neglect any size dependence and are biased by dependence on the signal-to-background ra...
journal_title:Nuclear medicine communications
pub_type: 临床试验,杂志文章
doi:10.1097/MNM.0b013e328155d154
更新日期:2007-06-01 00:00:00
abstract::Impaired cardiac fatty acid uptake, assessed by the radiolabelled fatty acid analogue beta-methyl-p-iodophenyl pentadecanoic acid (I-123-BMIPP), is observed in the myocardium following acute ischaemic events, but the long-term prognostic implication has not been established. This study aimed to determine the prognosti...
journal_title:Nuclear medicine communications
pub_type: 杂志文章,多中心研究
doi:10.1097/00006231-200010000-00003
更新日期:2000-10-01 00:00:00
abstract:OBJECTIVE:Determine the prevalence of benign indium-111 (In) pentetreotide uptake in the pancreatic head and determine if a semi-quantitative method can be used to differentiate physiologic from pathologic uptake. PATIENTS AND METHODS:Institutional Review Board-approved, HIPAA-compliant retrospective review of 197 som...
journal_title:Nuclear medicine communications
pub_type: 杂志文章
doi:10.1097/MNM.0000000000001022
更新日期:2019-07-01 00:00:00
abstract::Since the appearance of superficial tissue is often an unreliable indicator of deep tissue viability in cases of frostbite, radionuclide scintigraphy with 99Tcm-disodium oxidronate (HDP) was used to assess changes in tissue viability after experimental freezing and thawing of the rabbit ear. One shaved ear, left or ri...
journal_title:Nuclear medicine communications
pub_type: 杂志文章
doi:10.1097/00006231-199207000-00010
更新日期:1992-07-01 00:00:00
abstract:OBJECTIVE:The aim of our study was to explore the value of 3'-deoxy-3'-[F]fluorothymidine (F-FLT) and F-FLT PET in monitoring the early biologic response of esophageal carcinoma after irradiation in vitro and in vivo. METHODS:After 2, 4, and 8 h of irradiation at different doses (0, 5, 10, and 15 Gy) of esophageal car...
journal_title:Nuclear medicine communications
pub_type: 杂志文章
doi:10.1097/MNM.0000000000000201
更新日期:2014-12-01 00:00:00
abstract:OBJECTIVE:To verify the negative predictive value of pulmonary ventilation/perfusion scintigraphy with single photon emission computed tomography (V/Q SPECT) in ruling out pulmonary thromboembolism. METHODS:V/Q SPECT using 99mTc-Technegas was performed on 584 patients to rule out pulmonary thromboembolism between Octo...
journal_title:Nuclear medicine communications
pub_type: 临床试验,杂志文章
doi:10.1097/MNM.0b013e32827a8e99
更新日期:2007-08-01 00:00:00
abstract:BACKGROUND:Paediatric dual X-ray absorptiometry (DXA) studies present a number of technical problems. One of these is that the edge detection algorithms designed for the adult skeleton may fail for paediatric studies. Hologic provide alternative algorithms for low bone density studies. AIM:To assess low-density softwa...
journal_title:Nuclear medicine communications
pub_type: 临床试验,杂志文章
doi:10.1097/00006231-200506000-00002
更新日期:2005-06-01 00:00:00
abstract::Three ligand assays for TSH using monoclonal antibodies, an IRMA, an ELISA and a DELFIA were compared with a standard DAB RIA technique. The IRMA proved to be superior in respect to sensitivity, precision, reproducibility and practicality. The highest sensitivity yet, was achieved by DELFIA. IRMA, ELISA and DELFIA are...
journal_title:Nuclear medicine communications
pub_type: 杂志文章
doi:10.1097/00006231-198504000-00003
更新日期:1985-04-01 00:00:00
abstract:PURPOSE:To test the potential of a new reconstruction algorithm with Monte Carlo-based scatter correction in half-time myocardial perfusion single-photon emission computed tomography (SPECT). MATERIALS AND METHODS:The mathematical four-dimensional NURBS-based Cardiac-Torso phantom and the SIMIND Monte Carlo simulation...
journal_title:Nuclear medicine communications
pub_type: 杂志文章
doi:10.1097/MNM.0b013e328349c765
更新日期:2011-11-01 00:00:00
abstract::The ability of 99Tcm-methoxyisobutylisonitrile (MIBI) single photon emission tomography (SPET) to detect myocardial ischaemia and necrosis was assessed in 56 patients (45 male, 11 female, aged 55 +/- 5 years), with clinically recognized ischaemic heart disease (IHD). All underwent coronary angiography (CA) and left ve...
journal_title:Nuclear medicine communications
pub_type: 杂志文章
doi:10.1097/00006231-199212000-00004
更新日期:1992-12-01 00:00:00