Abstract：
BACKGROUND:Intestinal commensal flora seems to be a requisite for both human and experimental intestinal inflammation. Our aim was to assess the immunological changes in the colon of IL-10(-/-) mice depending on the environmental conditions. MATERIALS AND METHODS:Twelve wild-type (WT) and 24 IL-10(-/-) 4-week-old mice were kept under specific pathogen-free (SPF) conditions for 4 weeks. Half of them were transferred to a conventional environment. Mice were sacrificed at 12 weeks of age, and the incidence and severity of colitis was assessed. Intraepithelial (IEL) and lamina propria (LPL) lymphocytes were assessed for phenotype and apoptosis by flow cytometry. Toll-like receptors 2 (TLR2) and TLR9 expression was assessed by real-time PCR. Immunohistochemical analyses for cell apoptosis, TLR2 and MyD88 were also performed. RESULTS:IL-10(-/-) mice shifted to conventional conditions showed a greater incidence (66% vs. 50%) and severity of colitis than animals kept under SPF conditions (P = 0·009). The number of CD3+ IEL was higher and their apoptosis rate lower in IL-10(-/-) than in their WT counterparts, regardless of the environment. In LPL, however, these differences were only observed in mice shifted to conventional conditions. TLR2 expression was significantly increased in SPF-housed IL-10(-/-) mice when compared to WT controls. Immunohistochemistry demonstrated the loss of TLR2 and MyD88 in damaged areas. CONCLUSIONS:In SPF conditions, IL-10 deficiency appears to be compensated by an increased epithelial TLR2 expression, thus resulting in a milder colonic damage. However, in conventional conditions, this compensatory mechanism would be exceeded inducing a more severe colonic damage with activation of LPL immune cells.
journal_name
Eur J Clin Investjournal_title
European journal of clinical investigationauthors
Pedrosa E,Lorén V,Cabré E,Doménech E,Ojanguren I,Gassull MA,Mañé Jdoi
10.1111/j.1365-2362.2011.02499.xsubject
Has Abstractpub_date
2011-10-01 00:00:00pages
1047-53issue
10eissn
0014-2972issn
1365-2362journal_volume
41pub_type
杂志文章abstract：BACKGROUND:Inactivation of the tumour suppressor gene, p53, is the commonest genetic abnormality in human cancer. The study of the type of p53 mutation in a given tumour may provide prognostic information, clues to aetiology and become useful for therapeutics. MATERIALS AND METHODS:The molecular characterisation of p5...
journal_title：European journal of clinical investigation
pub_type： 杂志文章
doi：10.1046/j.1365-2362.2000.00717.x
更新日期：2000-09-01 00:00:00
abstract：:The aim of this research was to study exogenous prostacyclin effect on urinary kallikrein excretion (UKK) in man, to define whether prostacyclin-induced renin release and/or endogenously released cyclooxygenase products were responsible for prostacyclin-induced enhancement of UKK, to determine furosemide effect on UKK...
journal_title：European journal of clinical investigation
pub_type： 杂志文章
doi：10.1111/j.1365-2362.1986.tb01334.x
更新日期：1986-06-01 00:00:00
abstract：:Assuming that lithium is exclusively reabsorbed in the proximal tubules pari passu with sodium (Na), the lithium clearance (CLi) has been advanced as an index of filtrate delivery from the proximal tubules. In order to determine whether water loads in a range commonly given during clearance studies affect CLi, we stud...
journal_title：European journal of clinical investigation
pub_type： 杂志文章
doi：10.1111/j.1365-2362.1988.tb01258.x
更新日期：1988-06-01 00:00:00
abstract：:The concentrations of gastrins containing the active C-terminal tetrapeptide amide (mainly gastrin-34 and gastrin-17) and the N-terminal tridecapeptide fragment of gastrin-17 were measured in antral and duodenal biopsy specimens. The antral concentration of the N-terminal gastrin fragment was much higher in patients w...
journal_title：European journal of clinical investigation
pub_type： 杂志文章
doi：10.1111/j.1365-2362.1984.tb01126.x
更新日期：1984-06-01 00:00:00
abstract：:The aim of the present study was to examine the effects of a large dose of slow-release carbohydrates (CHOs) at bedtime on the nocturnal glucose, insulin and free fatty acid (FFA) levels, and to assess the putative effects on morning fasting and post-prandial glucose levels in patients with moderately controlled non-i...
journal_title：European journal of clinical investigation
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1046/j.1365-2362.1997.880639.x
更新日期：1997-02-01 00:00:00
abstract：:Renal extraction and renal plasma clearance of atrial natriuretic peptide from pigs with complete unilateral ureteral obstruction (UUO) and from intact anaesthetized pigs were determined from arteriovenous differences in plasma atrial natriuretic peptide and measured renal plasma flow. The effect of administration of ...
journal_title：European journal of clinical investigation
pub_type： 杂志文章
doi：10.1111/j.1365-2362.1995.tb01556.x
更新日期：1995-04-01 00:00:00
abstract：:Critical illness is an aetiologically and clinically heterogeneous syndrome that is characterised by organ failure and immune dysfunction. Mortality in critically ill patients is driven by inflammation-associated organ damage and a profound vulnerability to nosocomial infection. Both factors are influenced by the acti...
journal_title：European journal of clinical investigation
pub_type： 杂志文章,评审
doi：10.1111/eci.13028
更新日期：2018-12-01 00:00:00
abstract：BACKGROUND:Vascular endothelial growth factor (VEGF) helps control tumour growth via causing new capillaries growth in tumours. Four cardiac hormones [i.e. vessel dilator, long-acting natriuretic peptide (LANP), kaliuretic peptide (KP) and atrial natriuretic peptide (ANP)] that eliminate up to up to 86% of human small-...
journal_title：European journal of clinical investigation
pub_type： 杂志文章
doi：10.1111/j.1365-2362.2012.02695.x
更新日期：2012-10-01 00:00:00
abstract：BACKGROUND:Many haemodialysis patients treated with recombinant human erythropoietin (r-HuEPO) receive intravenous iron supplementation on a regular basis. It has been shown previously that this may result in a transient "oversaturation" of transferrin. METHODS:Ten stable haemodialysis patients on r-HuEPO treatment re...
journal_title：European journal of clinical investigation
pub_type： 临床试验,杂志文章
doi：10.1046/j.1365-2362.2002.0320s1036.x
更新日期：2002-03-01 00:00:00
abstract：BACKGROUND:Humming greatly increases nasal nitric oxide (NO) in healthy people by causing a rapid washout of NO from the sinuses. This increase is abolished in patients with complete sinus ostial obstruction. OBJECTIVE:Allergic rhinitis is a risk factor for development of sinusitis and we wanted to study whether nasal...
journal_title：European journal of clinical investigation
pub_type： 杂志文章
doi：10.1111/j.1365-2362.2004.01384.x
更新日期：2004-08-01 00:00:00
abstract：:Sporadic hypophosphataemic osteomalacia (adult-onset type) was demonstrated in a 40-year-old man on the basis of severe osteomalacia, hypophosphataemia, hyperphosphaturia and glycinuria. Plasma immunoreactive parathyroid hormone (iPTH) concentration was 9.3 ng prot./ml (normal range: 4-8 ng prot./ml). Plasma 25-hydrox...
journal_title：European journal of clinical investigation
pub_type： 杂志文章
doi：10.1111/j.1365-2362.1980.tb00047.x
更新日期：1980-10-01 00:00:00
abstract：:The aim of the present study was to investigate antral endocrine cell populations and tissue and circulating hormone levels following a 4-week oral regimen with prostaglandin E2 (25, 250 and 5000 micrograms/kg-1 b.i.d.) or a stable methyl analogue (5 and 50 micrograms kg-1 b.i.d.). Epithelial hyperplasia of the gastri...
journal_title：European journal of clinical investigation
pub_type： 杂志文章
doi：10.1111/j.1365-2362.1989.tb00209.x
更新日期：1989-04-01 00:00:00
abstract：BACKGROUND:Previous studies revealed that patients with Takotsubo syndrome (TTS) have a higher mortality rate than the general population and a comparable mortality to acute coronary syndrome (ACS). Repolarisation abnormalities, namely T-wave amplitude, may provide incremental prognostic information, in addition to tra...
journal_title：European journal of clinical investigation
pub_type： 杂志文章
doi：10.1111/eci.13078
更新日期：2019-04-01 00:00:00
abstract：BACKGROUND:We have investigated the potential for using antisense technology as a means of delivering treatment for acute myeloblastic leukaemia (FAB-M2) by gene therapy. MATERIALS AND METHODS:A test recombinant adenovirus vector was constructed containing human c-myc antisense fragments to study the effects of alteri...
journal_title：European journal of clinical investigation
pub_type： 杂志文章
doi：10.1111/j.1365-2362.2006.01589.x
更新日期：2006-01-01 00:00:00
abstract：BACKGROUND:Most of the previous reports found cirrhosis patients with a high risk of subsequent tuberculosis (TB). However, data about the risk of developing liver cirrhosis in TB patients are limited. As a hepatitis endemic area, the risk of liver cirrhosis in patients with TB should be elucidated in Taiwan. METHODS:...
journal_title：European journal of clinical investigation
pub_type： 杂志文章
doi：10.1111/eci.12453
更新日期：2015-07-01 00:00:00
abstract：:Primary biliary cirrhosis and primary sclerosing cholangitis are well recognized chronic cholestatic liver diseases that are considered to have an autoimmune basis. Recent progress in the study of autoimmune liver diseases has improved the recognition and characterization of these conditions. An important component of...
journal_title：European journal of clinical investigation
pub_type： 杂志文章,评审
doi：10.1046/j.1365-2362.2001.00765.x
更新日期：2001-01-01 00:00:00
abstract：:Currently available information on adipose tissue metabolism in man is mainly based on studies in vitro. In the present work adipose tissue lipid uptake was studied in vivo in man. 100 g glucose and 120 g milk fat with 10 microCi U-14C oleic acid were given in the overnight fasting state to eight women and 2 h after a...
journal_title：European journal of clinical investigation
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1111/j.1365-2362.1990.tb02263.x
更新日期：1990-04-01 00:00:00
abstract：:The clinical and physiological relevance of the relationship between gastrin and calcitonin has been investigated in normal subjects and in patients suffering from gastritis or duodenal ulcer. Basal plasma levels of calcitonin are increased in these patients but there is no significant relationship between calcitonin ...
journal_title：European journal of clinical investigation
pub_type： 临床试验,杂志文章
doi：10.1111/j.1365-2362.1981.tb02125.x
更新日期：1981-08-01 00:00:00
abstract：:Hyperthyroidism is associated with an increase in both osteoblast and osteoclast activity. We have previously shown that, in vitro, osteoclasts do not respond directly to tri-iodothyronine to increase bone resorption but that the effect is mediated by another bone cell, probably the osteoblast. To investigate this iss...
journal_title：European journal of clinical investigation
pub_type： 杂志文章
doi：10.1046/j.1365-2362.1996.160289.x
更新日期：1996-05-01 00:00:00
abstract：BACKGROUND:Acute myocardial infarction (AMI) is followed by post AMI cardiac remodelling, often leading to congestive heart failure. Homing of c-kit+ endothelial progenitor cells (EPC) has been thought to be the optimal source for regenerating infarcted myocardium. METHODS:Immune function of viable peripheral blood mo...
journal_title：European journal of clinical investigation
pub_type： 杂志文章
doi：10.1111/j.1365-2362.2009.02111.x
更新日期：2009-06-01 00:00:00
abstract：BACKGROUND:Chronic portal hypertension is associated with the development of portal-systemic collaterals. Long-term octreotide treatment has been shown to enhance the constrictive response to vasopressin in the mesenteric arteries of portal hypertensive rats. This study investigated the effects of long-term octreotide ...
journal_title：European journal of clinical investigation
pub_type： 杂志文章
doi：10.1046/j.1365-2362.2002.00987.x
更新日期：2002-05-01 00:00:00
abstract：BACKGROUND:It has been speculated that oral hypoglycaemic agents that block K-ATP channels could potentially increase blood pressure by blocking such channels in vascular myocytes. No information about this issue exists regarding nateglinide. DESIGN:A multicentre, double-blind, placebo-controlled, randomized trial was...
journal_title：European journal of clinical investigation
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1111/j.1365-2362.2007.01918.x
更新日期：2008-03-01 00:00:00
abstract：:Portal-systemic spill-over of unconjugated ursodeoxycholic acid (UDCA) was assessed in ten healthy subjects, six patients with mild chronic liver disease and eight patients with cirrhosis. Following oral administration of UDCA (1.5 mg/kg body weight), serum concentrations of unconjugated UDCA were measured during 2 h ...
journal_title：European journal of clinical investigation
pub_type： 杂志文章
doi：10.1111/j.1365-2362.1983.tb00126.x
更新日期：1983-12-01 00:00:00
abstract：:beta-Adrenoceptor binding on peripheral blood mononuclear cells (PBMC) of healthy adult volunteers was investigated using the radioligand 125iodo-cyanopindolol (ICYP). Saturation binding studies were performed with nine different concentrations of ICYP. Receptor density and affinity were calculated by Scatchard plots....
journal_title：European journal of clinical investigation
pub_type： 杂志文章
doi：10.1111/j.1365-2362.1988.tb02416.x
更新日期：1988-04-01 00:00:00
abstract：OBJECTIVES:EPB4.1 has been previously mapped to human chromosome 1p33-p34.2. In contradiction to this chromosomal location, we have mapped EPB4.1-1p36 by using fluorescence in situ hybridization and radiation hybrid mapping. In neuroblastomas, deletions of the telomeric end of chromosome 1 (1p36) are the most common ge...
journal_title：European journal of clinical investigation
pub_type： 杂志文章
doi：10.1046/j.1365-2362.2001.00892.x
更新日期：2001-10-01 00:00:00
abstract：BACKGROUND:Excess of cardiovascular risk among patients with chronic inflammatory diseases has been attributed to increased arterial stiffness. Hypercholesterolaemia has been demonstrated to promote a low-grade inflammatory status. The objective of the present study was to define, in hypercholesterolaemia, the influenc...
journal_title：European journal of clinical investigation
pub_type： 杂志文章
doi：10.1111/j.1365-2362.2004.01345.x
更新日期：2004-05-01 00:00:00
abstract：BACKGROUND:The transit time of the small intestine, in addition to lactase activity, may influence lactose digestion and thus play a role in the occurrence of lactose intolerance. The objectives of this study were to investigate the effect of lactose on the oro-cecal transit time (OCTT) in lactose digesters and maldige...
journal_title：European journal of clinical investigation
pub_type： 杂志文章
doi：10.1111/j.1365-2362.2006.01713.x
更新日期：2006-10-01 00:00:00
abstract：:The purpose of this work was to examine in an open, randomized parallel-group study whether an intervention programme directed towards hypercholesterolaemia, smoking and diabetes mellitus in treated hypertensive men was associated with less complex formation between low-density lipoprotein (LDL) and human arterial pro...
journal_title：European journal of clinical investigation
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1046/j.1365-2362.1996.2030543.x
更新日期：1996-11-01 00:00:00
abstract：BACKGROUND:Given that role of magnesium in insulin secretion is uncertain, our objective was to determine whether oral supplementation with magnesium chloride (MgCl(2)) improves the ability of beta-cells to compensate for variations in insulin sensitivity in non-diabetic individuals with significant hypomagnesaemia. M...
journal_title：European journal of clinical investigation
pub_type： 杂志文章,随机对照试验
doi：10.1111/j.1365-2362.2010.02422.x
更新日期：2011-04-01 00:00:00
abstract：BACKGROUND:Renal size and function reflect the health of the kidney. These parameters are associated with age, gender and body weight. The kidneys are also influenced by micro- and macrovascular diseases. Atherosclerotic markers and risk factors may influence the age-related changes of renal size and function. METHODS...
journal_title：European journal of clinical investigation
pub_type： 杂志文章
doi：10.1046/j.1365-2362.2003.01091.x
更新日期：2003-01-01 00:00:00
