Abstract:
:Venoms peptides have produced exceptional sources for drug development to treat pain. In this study we examined the antinociceptive and side effects of Tx3-3, a peptide toxin isolated from Phoneutria nigriventer venom, which inhibits high-voltage-dependent calcium channels (VDCC), preferentially P/Q and R-type VDCC. We tested the effects of Tx3-3 in animal models of nociceptive (tail-flick test), neuropathic (partial sciatic nerve ligation and streptozotocin-induced diabetic neuropathy), and inflammatory (intraplantar complete Freund's adjuvant) pain. In the tail-flick test, both intrathecal (i.t.) and intracerebroventricular (i.c.v.) injection of Tx3-3 in mice caused a short-lasting effect (ED(50) and 95% confidence intervals of 8.8 [4.1-18.8] and 3.7 [1.6-8.4] pmol/site for i.t. and i.c.v. injection, respectively), without impairing motor functions, at least at doses 10-30 times higher than the effective dose. By comparison, ω-conotoxin MVIIC, a P/Q and N-type VDCC blocker derived from Conus magus venom, caused significant motor impairment at doses close to efficacious dose in tail flick test. Tx3-3 showed a long-lasting antinociceptive effect in neuropathic pain models. Intrathecal injection of Tx3-3 (30 pmol/site) decreased both mechanical allodynia produced by sciatic nerve injury in mice and streptozotocin-induced allodynia in mice and rats. On the other hand, i.t. injection of Tx3-3 did not alter inflammatory pain. Taken together, our data show that Tx3-3 shows prevalent antinociceptive effects in the neuropathic pain models and does not cause adverse motor effects at antinociceptive efficacious doses, suggesting that this peptide toxin holds promise as a novel therapeutic agent for the control of neuropathic pain. The Brazilian armed spider Tx3-3, a new P/Q and R-type calcium channel blocker, effectively alleviates allodynia in animal neuropathic pain models.
journal_name
Painjournal_title
Painauthors
Dalmolin GD,Silva CR,Rigo FK,Gomes GM,do Nascimento Cordeiro M,Richardson M,Silva MAR,Prado MAM,Gomez MV,Ferreira Jdoi
10.1016/j.pain.2011.04.015subject
Has Abstractpub_date
2011-10-01 00:00:00pages
2224-2232issue
10eissn
0304-3959issn
1872-6623pii
00006396-201110000-00011journal_volume
152pub_type
杂志文章相关文献
PAIN文献大全abstract::The opiate antagonist naloxone has been reported to cause pain relief. Therefore, the effect was determined of naloxone, injected intravenously, on the activity in single neurones of the dorsomedial part of the ventral nucleus (VDM) in the thalamus of rats under urethane anaesthesia elicited by electrical stimulation ...
journal_title:Pain
pub_type: 杂志文章
doi:10.1016/0304-3959(88)90144-3
更新日期:1988-12-01 00:00:00
abstract::Limited information on the prevalence and risk factors for chronic pain is available for developing countries. Therefore, we investigated the prevalence of chronic pain and the association between this pain and various personal and sociodemographic factors by including questions in the South Africa Demographic and Hou...
journal_title:Pain
pub_type: 杂志文章
doi:10.1097/j.pain.0000000000001844
更新日期:2020-07-01 00:00:00
abstract::The mechanisms of chronic pain in irritable bowel syndrome (IBS) have been widely investigated but remain unclear. The present study investigated the relation between visceral hypersensitivity, cutaneous thermal sensitivity, and central pain mechanisms. Rectal sensitivity was assessed with a barostat, and forearm and ...
journal_title:Pain
pub_type: 杂志文章
doi:10.1016/j.pain.2009.10.005
更新日期:2010-01-01 00:00:00
abstract::Many cancerous solid tumors metastasize to the bone and induce pain (cancer-induced bone pain [CIBP]). Cancer-induced bone pain is often severe because of enhanced inflammation, rapid bone degradation, and disease progression. Opioids are prescribed to manage this pain, but they may enhance bone loss and increase tumo...
journal_title:Pain
pub_type: 杂志文章
doi:10.1097/j.pain.0000000000000690
更新日期:2016-12-01 00:00:00
abstract::The present study aimed to investigate if (1) subcutaneous injection of glutamate induces pain, sensitization and vasomotor responses in humans and (2) if sex differences exist in these responses. Thirty healthy volunteers (men-15 and women-15) were included. Each subject received four subcutaneous injections (0.1ml; ...
journal_title:Pain
pub_type: 杂志文章
doi:10.1016/j.pain.2006.06.015
更新日期:2006-10-01 00:00:00
abstract::While a variety of cultural, psychological and physiological factors contribute to variability in both clinical and experimental contexts, the role of genetic factors in human pain sensitivity is increasingly recognized as an important element. This study was performed to evaluate genetic influences on variability in ...
journal_title:Pain
pub_type: 杂志文章
doi:10.1016/j.pain.2004.02.027
更新日期:2004-06-01 00:00:00
abstract::Chronic pain not only interferes with daily activities, it may also have a negative impact on the perceived integrity of one's self through self-discrepancies. Self-discrepancies are experienced distances between the actual self and self-guides that can exist from 2 perspectives (ie, own and other). Self-discrepancies...
journal_title:Pain
pub_type: 杂志文章
doi:10.1016/j.pain.2010.11.009
更新日期:2011-02-01 00:00:00
abstract::This laboratory-based study examined lagged associations between child pain behavior and maternal responses as a function of maternal catastrophizing (CAT). Mothers completed the parent version of the Pain Catastrophizing Scale. Children participated in a validated water ingestion procedure to induce abdominal discomf...
journal_title:Pain
pub_type: 杂志文章
doi:10.1097/j.pain.0000000000000963
更新日期:2017-09-01 00:00:00
abstract::Genetic polymorphisms result in absent enzyme activity of CYP2D6 (poor metabolizers, PM) in about 10% of the Caucasian population. This study investigates whether the PM genotype has an impact on the response to tramadol analgesia in postoperative patients. A prospective study design was used and 300 patients recoveri...
journal_title:Pain
pub_type: 杂志文章
doi:10.1016/s0304-3959(03)00212-4
更新日期:2003-09-01 00:00:00
abstract::The present study examined antinociception produced by systemic administration of caffeine in the rat hot-plate (HP) and formalin tests and addressed several aspects of the mechanism of action of caffeine. Locomotor activity was monitored throughout. Caffeine produced a dose-related antinociception the HP (50-100 mg/k...
journal_title:Pain
pub_type: 杂志文章
doi:10.1016/0304-3959(94)00169-F
更新日期:1995-05-01 00:00:00
abstract::Transient receptor potential ion channel melastatin subtype 8 (TRPM8) is activated by cold temperatures and cooling agents, such as menthol and icilin. Compounds containing peppermint are reported to reduce symptoms of bowel hypersensitivity; however, the underlying mechanisms of action are unclear. Here we determined...
journal_title:Pain
pub_type: 杂志文章
doi:10.1016/j.pain.2011.01.027
更新日期:2011-07-01 00:00:00
abstract::Adults with mood disorders frequently use prescription opioids. The factors associated with this increased use remain unclear. We used the Medical Expenditure Panel Surveys from 2005 to 2011 to measure the association of mood disorders with new opioid use and the transition to longer-term opioid use for a variety of p...
journal_title:Pain
pub_type: 杂志文章
doi:10.1097/j.pain.0000000000000650
更新日期:2016-11-01 00:00:00
abstract::Chronic pain is currently considered a public health problem with high costs to the individual and society. To improve prevention and treatment of chronic pain, epidemiologic studies are mandatory for assessing chronic pain. The aims of this study were to estimate the prevalence of chronic pain in the adult Danish pop...
journal_title:Pain
pub_type: 杂志文章
doi:10.1016/j.pain.2012.07.023
更新日期:2012-12-01 00:00:00
abstract::The responses of preterm neonates to acute tissue-damaging stimuli have been described. However, factors which influence these responses have received little attention. In this study, we observed 124 premature infants before, during and after a routine heel lance and determined how two contextual variables (severity o...
journal_title:Pain
pub_type: 临床试验,杂志文章
doi:10.1016/0304-3959(94)90053-1
更新日期:1994-10-01 00:00:00
abstract::Chronic pain is associated with large societal costs, but few studies have investigated the total costs of chronic pain with respect to elderly subjects. The elderly usually require informal care, care performed by municipalities, and care for chronic diseases, all factors that can result in extensive financial burden...
journal_title:Pain
pub_type: 杂志文章
doi:10.1097/01.j.pain.0000460336.31600.01
更新日期:2015-03-01 00:00:00
abstract::The purpose of this study was to determine if some commonly used admission variables could predict treatment outcome on a multidisciplinary pain center. Specifically, it was designed as a prospective study of an index proposed by Maruta et al. The subjects were 104 patients. The 4 outcome measures used were staff judg...
journal_title:Pain
pub_type: 杂志文章
doi:10.1016/0304-3959(82)90082-3
更新日期:1982-09-01 00:00:00
abstract::Quantitative sensory testing of heat pain sensation has become an important tool to evaluate small caliber afferent nerve function in peripheral neuropathy. In earlier studies, we found that topical application of capsaicin in humans results in the loss of epidermal nerve fibers (ENFs) with a corresponding decrease in...
journal_title:Pain
pub_type: 临床试验,杂志文章
doi:10.1016/S0304-3959(00)00444-9
更新日期:2001-04-01 00:00:00
abstract::Cyclin-dependent kinase 5 (Cdk5) is a unique member of the CDK family. It is predominantly expressed in postmitotic neurons and has been implicated in neuronal plasticity. The present study showed that Cdk5 and p35 were expressed in primary sensory and dorsal horn neurons, while p25, an N-terminal truncated derivative...
journal_title:Pain
pub_type: 杂志文章
doi:10.1016/j.pain.2006.08.008
更新日期:2007-01-01 00:00:00
abstract::We previously reported that morphine fails to produce analgesic tolerance when administered in the presence of formalin-induced pain. The hypothalamic-pituitary-adrenal (HPA) axis is known to respond to stressful stimuli, including pain. To examine whether the blockade of tolerance by pain is related to HPA activity, ...
journal_title:Pain
pub_type: 杂志文章
doi:10.1016/0304-3959(95)00069-0
更新日期:1995-12-01 00:00:00
abstract::Back pain is a leading cause of disability. Previous research suggests that modifiable risk factors influence recovery from back pain, and practice guidelines recommend integrating such factors within primary care management. Toward this goal, a brief, multidimensional questionnaire, the STarT Back Tool, was designed ...
journal_title:Pain
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.pain.2012.06.010
更新日期:2012-11-01 00:00:00
abstract::The spinal ascending pathways responsible for neuronal ventrobasal (VB) thalamic responses elicited by joint stimulation of the posterior paw were determined in arthritic rats used as a model of experimental pain. Responses of a same neurone to mechanical (movement--pressure--brushing) or thermal stimulation (50 degre...
journal_title:Pain
pub_type: 杂志文章
doi:10.1016/0304-3959(86)90059-X
更新日期:1986-09-01 00:00:00
abstract::Pain is a complex human trait. It is likely that the interaction of multiple genes, each with a small individual effect, along with the effect of environmental factors, influences the clinical efficacy of opioids rather than a single gene alone. Polymorphisms in genes coding for the mu-opioid receptor (A118G) and cate...
journal_title:Pain
pub_type: 杂志文章
doi:10.1016/j.pain.2006.10.023
更新日期:2007-07-01 00:00:00
abstract::Immune activation near healthy peripheral nerves may have a greater role in creating pathological pain than previously recognized. We have developed a new model of sciatic inflammatory neuritis to assess how such immune activation may influence somatosensory processing. The present series of experiments reveal that zy...
journal_title:Pain
pub_type: 杂志文章
doi:10.1016/S0304-3959(01)00354-2
更新日期:2001-12-01 00:00:00
abstract::Tibia fracture induces exaggerated substance P (SP) and calcitonin gene-related peptide (CGRP) signaling and neuropeptide-dependent nociceptive and inflammatory changes in the hind limbs of rats similar to those seen in complex regional pain syndrome. Inflammatory changes in the spinal cord contribute to nociceptive s...
journal_title:Pain
pub_type: 杂志文章
doi:10.1097/j.pain.0000000000000204
更新日期:2015-10-01 00:00:00
abstract::Reports of pain during mammography show that there is great variability in both the incidence of reported pain (0.2-62%) and the intensity of that pain. Much of that variability may be due to the measures used to rate mammography pain. This is the first study that has examined the incidence, quality and intensity of m...
journal_title:Pain
pub_type: 临床试验,杂志文章
doi:10.1016/0304-3959(96)03057-6
更新日期:1996-08-01 00:00:00
abstract::Part I of our earlier pilot study demonstrated that patients preferred modulated stimulation forms - frequency modulation and burst - rather than conventional continuous mode. To assess whether long-term therapeutic effects validate the immediate test results, this trial was performed in 14 patients with 21 pain condi...
journal_title:Pain
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/0304-3959(91)90200-H
更新日期:1991-11-01 00:00:00
abstract::The present study examined the relationship between depression and a constellation of pain-related variables that describe the experience of chronic pain patients. Thirty-seven depressed and 32 non-depressed heterogeneous chronic pain patients were identified through structured interviews, use of standardized criteria...
journal_title:Pain
pub_type: 杂志文章
doi:10.1016/0304-3959(91)90073-7
更新日期:1991-08-01 00:00:00
abstract::Pain modulation by placebo mechanisms is one of the most robust and best-studied phenomena, yet almost all research investigating the mechanisms and implications of the placebo analgesia are based on adult research. After highlighting crucial aspects that need to be considered in studying pain modulation in children, ...
journal_title:Pain
pub_type: 杂志文章,评审
doi:10.1016/j.pain.2014.08.036
更新日期:2014-11-01 00:00:00
abstract::A large body of evidence indicates that nitric oxide (NO)/cGMP signaling essentially contributes to the processing of chronic pain. In general, NO-induced cGMP formation is catalyzed by 2 isoforms of guanylyl cyclase, NO-sensitive guanylyl cyclase 1 (NO-GC1) and 2 (NO-GC2). However, the specific functions of the 2 iso...
journal_title:Pain
pub_type: 杂志文章
doi:10.1097/j.pain.0000000000001440
更新日期:2019-03-01 00:00:00
abstract::It is well known that acupuncture has pain-relieving effects, but the contribution of specific and especially nonspecific factors to acupuncture analgesia is less clear. One hundred one patients who developed pain of ≥ 3 on a visual analog scale (VAS, 0 to 10) after third molar surgery were randomized to receive activ...
journal_title:Pain
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.pain.2013.05.008
更新日期:2013-09-01 00:00:00