Abstract：
:We present here a Sleeping Beauty-based transposition system that offers a simple and efficient way to investigate the regulatory architecture of mammalian chromosomes in vivo. With this system, we generated several hundred mice and embryos, each with a regulatory sensor inserted at a random genomic position. This large sampling of the genome revealed the widespread presence of long-range regulatory activities along chromosomes, forming overlapping blocks with distinct tissue-specific expression potentials. The presence of tissue-restricted regulatory activities around genes with widespread expression patterns challenges the gene-centric view of genome regulation and suggests that most genes are modulated in a tissue-specific manner. The local hopping property of Sleeping Beauty provides a dynamic approach to map these regulatory domains at high resolution and, combined with Cre-mediated recombination, allows for the determination of their functions by engineering mice with specific chromosomal rearrangements.
journal_name
Nat Genetjournal_title
Nature geneticsauthors
Ruf S,Symmons O,Uslu VV,Dolle D,Hot C,Ettwiller L,Spitz Fdoi
10.1038/ng.790subject
Has Abstractpub_date
2011-03-20 00:00:00pages
379-86issue
4eissn
1061-4036issn
1546-1718pii
ng.790journal_volume
43pub_type
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