LY96, UPKIB mutations and TLR4, CD14, MBL polymorphisms in children with urinary tract infection.

Abstract:

OBJECTIVE:To evaluate genetic variations of innate immune system such as mannose binding lectin (MBL), Toll like receptor 4 (TLR4), CD14, LY96 (MD2) and Uroplakin 1B (UPK1B) genes in children with recurrent urinary tract infection (UTI). METHODS:The study included 30 children with recurrent UTI and 30 healthy controls. Blood was drawn and analysed for genetic polymorphisms of MBL, TLR4 and CD14 genes by the PCR-RFLP method. Direct DNA sequencing analysis was performed for LY96 and UPK 1B gene mutation in 10 children from UTI group and 5 children from control group. RESULTS:TLR4 gene Thr399Ile polymorphism was not observed in any child. Genotype distribution and allele frequency of Asp299Gly polymorphism was similar in both groups (p = 0.55). Codon 54 polymorphism of the MBL gene was similar in UTI and control groups (p = 0.49). -159 CC/CT/TT genotypes of CD14 gene was similar between the two groups (p = 0.14). UPK1B and LY96 gene DNA sequence analysis was similar in UTI and control groups. CONCLUSIONS:This study is the first study in which different parts of the innate immune system were evaluated in UTI etiopathogenesis in Turkish children. The results did not point out a significant role of any of the genes evaluated in this study.

journal_name

Indian J Pediatr

authors

Ertan P,Berdeli A,Yilmaz O,Gonulal DA,Yuksel H

doi

10.1007/s12098-011-0399-8

subject

Has Abstract

pub_date

2011-10-01 00:00:00

pages

1229-33

issue

10

eissn

0019-5456

issn

0973-7693

journal_volume

78

pub_type

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