Analysis of the urinary peptidome associated with Helicobacter pylori infection.

Abstract:

AIM:To investigate the relationship between urinary peptide changes and Helicobacter pylori (H. pylori) infection using urinary peptidome profiling. METHODS:The study was performed in volunteers (n=137) who gave informed consent. Urinary peptides were enriched by magnetic beads based weak cation exchange chromatography and spectrums acquired by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS). ClinProTools bioinformatics software was used for statistical analysis and the recognition of peptide patterns. The marker peptides were identified by LTQ Obitrap XL tandem MS. RESULTS:Approximately 50 proteins or peptides which loaded onto the magnetic beads were detected by MALDI-TOF MS. By optimizing the parameters of the model, the Genetic Algorithm model had good recognition capability (97%) and positive predictive value (94%). Based on the model, 2 markers with molecular masses of 6788 and 1912 Da were found that differentiated between H. pylori positive and negative volunteers. The m/z 1912 sequence was parsed as SKQFTSSTSYNRGDSTF. The peptide was identified as isoform 1 of the fibrinogen α chain precursor, whose concentration in urine was markedly higher in H. pylori infected volunteers than in H. pylori non-infected ones. CONCLUSION:The appearance of urinary fibrinogen degradation products is caused by an active H. pylori-induced process.

journal_name

World J Gastroenterol

authors

Xiao D,Meng FL,He LH,Gu YX,Zhang JZ

doi

10.3748/wjg.v17.i5.618

subject

Has Abstract

pub_date

2011-02-07 00:00:00

pages

618-24

issue

5

eissn

1007-9327

issn

2219-2840

journal_volume

17

pub_type

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