Human microRNA hsa-miR-125a-5p interferes with expression of hepatitis B virus surface antigen.

abstract：:REV1 and DNA Polymerase ζ (REV3 and REV7) play important roles in translesion DNA synthesis (TLS) in which DNA replication bypasses blocking lesions. REV1 and Polζ have also been implicated in promoting repair of DNA double-stranded breaks (DSBs). However, the mechanism by which these two TLS polymerases increase tole...

journal_title：Nucleic acids research

authors： Sharma S,Hicks JK,Chute CL,Brennan JR,Ahn JY,Glover TW,Canman CE

更新日期：2012-01-01 00:00:00

abstract：:Translesional DNA synthesis past abasic sites proceeds with the preferential incorporation of dAMP opposite the lesion and, depending on the sequence context, one or two base deletions. High-resolution NMR spectroscopy and molecular dynamics simulations were used to determine the three-dimensional structure of a DNA h...

journal_title：Nucleic acids research

authors： Lin Z,Hung KN,Grollman AP,de los Santos C

更新日期：1998-05-15 00:00:00

abstract：:The purine rich oligodeoxyribonucleotides 1C, d(ATGACGGAATA) and 2C, d(ATGAGCGAATA) alone exhibit highly cooperative melting transitions. Analysis of the concentration dependence of melting, and electrophoretic studies indicate that these oligomers can form an unusual purine rich offset double helix. The unusual duple...

journal_title：Nucleic acids research

authors： Wilson WD,Dotrong MH,Zuo ET,Zon G

更新日期：1988-06-10 00:00:00

abstract：:The titration viscometric investigation of the multi-mode interaction of netropsin (Nt) with (A.T) clusters of NaDNA12 and NH4DNA10 has been extended to different temperatures. The position of two boundaries on the r-scale (r= [Nt]bound/[DNA-P]) with increasing temperature steadily (rI/II) or more abruptly (rO/I) shif...

journal_title：Nucleic acids research

authors： Reinert KE,Geller D,Stutter E

更新日期：1981-05-25 00:00:00

abstract：:A filter binding assay was developed that allows measurement of specific binding of trp repressor to operator DNA. The most important feature of this procedure is the concentration and type of salt present in the binding buffer. Using this assay the dissociation constant of the repressor-operator complex was determine...

journal_title：Nucleic acids research

authors： Klig LS,Crawford IP,Yanofsky C

更新日期：1987-07-10 00:00:00

abstract：:Synchronized cells of a normal human lymphocytic cell line contain little swiven enzyme activity in G0 and G1 and high activity in Sphase. The level of activity in different growth phase appears to be related to the fraction of the population engaged in DNA replication. No endogenous inhibitor or activator of swiven a...

journal_title：Nucleic acids research

authors： Rosenberg BH,Ungers G,Deutsch JF

更新日期：1976-12-01 00:00:00

abstract：:In DNA of the dinoflagellate Crypthecodinium cohnii, 38% of the thymine is replaced by the modified base 5-hydroxymethyluracil, and approximately 3% of the cytosine is replaced by 5-methylcytosine. Both of the modified bases are non-randomly distributed in the DNA. Determinations of 3' nearest neighbors show that HOMe...

journal_title：Nucleic acids research

authors： Steele RE,Rae PM

更新日期：1980-10-24 00:00:00

abstract：:PvuRts1I is a prototype for a larger family of restriction endonucleases that cleave DNA containing 5-hydroxymethylcytosine (5hmC) or 5-glucosylhydroxymethylcytosine (5ghmC), but not 5-methylcytosine (5mC) or cytosine. Here, we report a crystal structure of the enzyme at 2.35 Å resolution. Although the protein has bee...

journal_title：Nucleic acids research

authors： Kazrani AA,Kowalska M,Czapinska H,Bochtler M

更新日期：2014-05-01 00:00:00

abstract：:To explain the activation of transcription by a remote enhancer, two models are most often considered, namely looping and scanning. A scanning model, also referred to as 'polymerase entry site' model predicts that for two adjacent promoters the one proximal to an enhancer would be preferentially activated. Preferentia...

journal_title：Nucleic acids research

authors： Heuchel R,Matthias P,Schaffner W

更新日期：1989-11-25 00:00:00

abstract：:We have sequenced the Escherichia coli grpE gene and shown that it encodes a 197-amino acid residue protein of 21,668-Mr. The predicted N-terminal amino acid sequence, as well as the overall amino acid composition agree well with that of the purified protein. From Northern analysis, we have shown that transcription of...

journal_title：Nucleic acids research

authors： Lipinska B,King J,Ang D,Georgopoulos C

更新日期：1988-08-11 00:00:00

abstract：:We have examined the ability of a cationic planar chromophore, ethidium bromide, to intercalate into a short, defined triple helix. Using UV absorption, fluorescence spectroscopy and a gel retardation assay we demonstrate that ethidium bromide is able to bind to a triple helix with a lower affinity than to the corresp...

journal_title：Nucleic acids research

authors： Mergny JL,Collier D,Rougée M,Montenay-Garestier T,Hélène C

更新日期：1991-04-11 00:00:00

abstract：:Homing endonuclease genes (HEGs) are mobile DNA elements that are thought to confer no benefit to their host. They encode site-specific DNA endonucleases that perpetuate the element within a species population by homing and disseminate it between species by horizontal transfer. Several yeast species contain the VMA1 H...

journal_title：Nucleic acids research

authors： Posey KL,Koufopanou V,Burt A,Gimble FS

更新日期：2004-07-27 00:00:00

abstract：:From a library of nucleic acid molecules, which are randomized in parts of their sequence, unique sequence variants can be selected for specific properties. The planning of such an in vitro selection experiment requires some consideration regarding how much DNA template or RNA transcript should be used initially. The ...

journal_title：Nucleic acids research

authors： Tabler M,Benos P,Dörr M

更新日期：1996-09-01 00:00:00

abstract：:A novel method to measure mRNA levels has been developed by combining the detection capabilities of RNase protection (RPA) with the quantification advantages of scintillation proximity assay (SPA) technology. Sample processing is reduced to the addition of a single reagent post RNase digestion. As a model system, the ...

journal_title：Nucleic acids research

authors： Kenrick MK,Jiang L,Potts CL,Owen PJ,Shuey DJ,Econome JG,Anson JG,Quinet EM

更新日期：1997-07-15 00:00:00

abstract：:Single-nucleotide variation and gene expression of disease samples represent important resources for biomarker discovery. Many databases have been built to host and make available such data to the community, but these databases are frequently limited in scope and/or content. BioMuta, a database of cancer-associated si...

journal_title：Nucleic acids research

authors： Dingerdissen HM,Torcivia-Rodriguez J,Hu Y,Chang TC,Mazumder R,Kahsay R

更新日期：2018-01-04 00:00:00

abstract：:The unknown modified nucleotide G*, isolated from both Schizosaccharomyces pombe and Torulopsis utilis initiator tRNAs(Met), has been identified as an O-ribosyl-(1"----2')-guanosine-5"-phosphate, called Gr(p), by means of HPLC, UV-absorption, mass spectrometry and periodate oxidation procedures. By comparison with the...

journal_title：Nucleic acids research

authors： Glasser AL,Desgres J,Heitzler J,Gehrke CW,Keith G

更新日期：1991-10-11 00:00:00

abstract：:The human beta-globin LCR plays a key role in the transcriptional regulation of the beta-globin locus and comprises four erythroid specific DNase I hypersensitive sites, designated 5'HS1-4. We have now isolated genomic clones containing 5'HS3 and 5'HS4 of the mouse beta-globin LCR. 5'HS3 and 5'HS4 are located 15 kb an...

journal_title：Nucleic acids research

authors： Jiménez G,Gale KB,Enver T

更新日期：1992-11-11 00:00:00

abstract：:We analyzed the effects of site-directed mutations in the SUC2 promoter of Saccharomyces cerevisiae. Analyses were performed in wild-type as well as mig1 and tup1 mutant strains after the promoter mutants were reintroduced into the native SUC2 locus on the left arm of chromosome IX. Mutation of the two GC boxes reveal...

journal_title：Nucleic acids research

authors： Bu Y,Schmidt MC

更新日期：1998-02-15 00:00:00

abstract：:Transcription of the human adenovirus 5 genome in transformed rat embryo cells (DFK3) was investigated using two different approaches. Preferential digestion of transcribed viral sequences by DNase I was analysed using kinetics of renaturation of 32P-labeled Ad5 HpaI restriction fragments in the presence of material w...

journal_title：Nucleic acids research

authors： Frolova EI,Zalmanzon ES,Lukanidin EM,Georgiev GP

更新日期：1978-01-01 00:00:00

abstract：:We have isolated a unique fragment of the HLA-DR alpha gene and probed human genomic DNA at low stringency to search for homologous sequences. A minimum of six non-polymorphic cross-hybridizing high molecular weight fragments were found in all DNAs examined. In order to obtain molecular clones of these cross-hybridizi...

journal_title：Nucleic acids research

authors： Lawrance SK,Das HK,Pan J,Weissman SM

更新日期：1985-10-25 00:00:00

abstract：:Mutational analysis of the 5'-untranslated leader sequence (omega) of tobacco mosaic virus (TMV) was carried out to determine those sequences necessary for the translational enhancement associated with omega. Five deletion mutants, a single base substitution, and a 25 base replacement mutant were tested for alteration...

journal_title：Nucleic acids research

authors： Gallie DR,Sleat DE,Watts JW,Turner PC,Wilson TM

更新日期：1988-02-11 00:00:00

abstract：:Laser microirradiation is a powerful tool for real-time single-cell analysis of the DNA damage response (DDR). It is often found, however, that factor recruitment or modification profiles vary depending on the laser system employed. This is likely due to an incomplete understanding of how laser conditions/dosages affe...

journal_title：Nucleic acids research

authors： Saquilabon Cruz GM,Kong X,Silva BA,Khatibzadeh N,Thai R,Berns MW,Yokomori K

更新日期：2016-02-18 00:00:00

abstract：:The nucleotide sequences 3' to the translational termination codons in a collection of human genes have been analysed for evidence of a preferred 3' context for natural UAG codons. The aim was to see whether human UAG contexts can be related to the recent demonstration of the effects of 3' context on nonsense suppress...

journal_title：Nucleic acids research

authors： Martin R

更新日期：1994-01-11 00:00:00

abstract：:The oncoprotein c-Myc plays an important role in cell proliferation, transformation, inhibition of differentiation and apoptosis. These functions most likely result from the transcription factor activity of c-Myc. As a heterodimer with Max, the c-Myc protein binds to the E-box sequence (CACGTG), which is also recogniz...

journal_title：Nucleic acids research

authors： Walhout AJ,Gubbels JM,Bernards R,van der Vliet PC,Timmers HT

更新日期：1997-04-15 00:00:00

abstract：:The synthesis of a pentadecamer of the 5'-phosphate of the antiviral nucleoside ribavirin (1'-beta-D-ribofuranosyl-1H-1,2,4-triazole-3-carboxamide) has been achieved. This homoribopolymer is terminated at the 5'-position with an (6-aminohexyl)phosphate group to permit conjugation to a carrier and at the 3'-position by...

journal_title：Nucleic acids research

authors： Dawson MI,Jina AN,Torkelson S,Rhee S,Moore M,Zarling DA,Hobbs PD

更新日期：1990-03-11 00:00:00

abstract：:The plastid psbA promoter of tobacco was used with the aim to construct plastid specific marker genes. Upon transfer to the tobacco nuclear genome the plastid promoter fragment appeared to specify a messenger RNA. Placed 5' to the bar or nptII coding sequences the level of expression is sufficient to obtain a selectab...

journal_title：Nucleic acids research

authors： Cornelissen M,Vandewiele M

更新日期：1989-01-11 00:00:00

abstract：:The RESID Database is a comprehensive collection of annotations and structures for protein post-translational modifications including N-terminal, C-terminal and peptide chain cross-link modifications. The RESID Database includes systematic and frequently observed alternate names, Chemical Abstracts Service registry nu...

journal_title：Nucleic acids research

authors： Garavelli JS,Hou Z,Pattabiraman N,Stephens RM

更新日期：2001-01-01 00:00:00

abstract：:We devised an indicator gene for retrotransposition based on an autocatalytic ribozyme element--the Tetrahymena thermophila 23S rRNA group I intron--which can self-splice in vitro and does not require--at variance with nuclear mRNA introns--any specific pathway and cellular component for the completion of the splicing...

journal_title：Nucleic acids research

authors： Esnault C,Casella JF,Heidmann T

更新日期：2002-06-01 00:00:00

abstract：:DNA polymerase III (DNA pol III) efficiently replicates the Escherichia coli genome, but it cannot bypass DNA damage. Instead, translesion synthesis (TLS) DNA polymerases are employed to replicate past damaged DNA; however, the exchange of replicative for TLS polymerases is not understood. The umuD gene products, whic...

journal_title：Nucleic acids research

authors： Silva MC,Nevin P,Ronayne EA,Beuning PJ

更新日期：2012-07-01 00:00:00

abstract：:Protein-DNA recognition is a critical component of gene regulatory processes but the underlying molecular mechanisms are not yet completely understood. Whereas the DNA binding preferences of transcription factors (TFs) are commonly described using nucleotide sequences, the 3D DNA structure is recognized by proteins an...

journal_title：Nucleic acids research

authors： Dror I,Zhou T,Mandel-Gutfreund Y,Rohs R

更新日期：2014-01-01 00:00:00