Abstract:
:The mouse pro-B cell line Ba/F3 has gained major interest as a model system to investigate oncogenic tyrosine kinases and to determine the efficacy of kinase inhibitors. While Ba/F3 cells are suitable to study oncogenic kinases derived from various cell types, the signaling networks in Ba/F3 cells are B-cell specific. We have established a mouse CD4+CD8+ double positive T-cell line (named MOHITO, for MOuse Hematopoietic Interleukin-dependent cell line of T-cell Origin) that has many features of human T-cell acute lymphoblastic leukemia (Notch1 and Jak1 mutation, TCR rearrangement) and is dependent on interleukin-7. The MOHITO cell line can be transformed to cytokine independent proliferation by BCR-ABL1 or mutant JAK1. This mouse T-cell line is a novel model system to investigate protein signaling and inhibition in a T-cell specific context and is a valuable tool to study and verify oncogenic capacity of mutations in the kinome and phosphatome in T-cell malignancies.
journal_name
Haematologicajournal_title
Haematologicaauthors
Kleppe M,Mentens N,Tousseyn T,Wlodarska I,Cools Jdoi
10.3324/haematol.2010.035931subject
Has Abstractpub_date
2011-05-01 00:00:00pages
779-83issue
5eissn
0390-6078issn
1592-8721pii
haematol.2010.035931journal_volume
96pub_type
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