The white-rot fungus Pleurotus ostreatus secretes laccase isozymes with different substrate specificities.

Abstract:

:Four laccase isozymes (LCC1, LCC2, LCC3 and LCC4) synthesized by Pleurotus ostreatus strain V-184 were purified and characterized. LCC1 and LCC2 have molecular masses of about 60 and 65 kDa and exhibited the same pI value (3.0). Their N termini were sequenced, revealing the same amino acid sequence and homology with laccases from other microorganisms. Laccases LCC3 and LCC4 were characterized by SDS-PAGE, estimating their molecular masses around 80 and 82 kDa, respectively. By native isoelectrofocusing, their pI values were 4.7 and 4.5, respectively. When staining with ABTS and guaiacol in native polyacrilamide gels, different specificities were observed for LCC1/LCC2 and LCC3/LCC4 isozymes.

journal_name

Mycologia

journal_title

Mycologia

authors

Mansur M,Arias ME,Copa-Patiño JL,Flärdh M,González AE

doi

10.1080/15572536.2004.11833017

subject

Has Abstract

pub_date

2003-11-01 00:00:00

pages

1013-20

issue

6

eissn

0027-5514

issn

1557-2536

pii

95/6/1013

journal_volume

95

pub_type

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