Abstract:
:We present a strategy to recover high molecular weight genomic DNA from large numbers of isolates of Phytophthora. Included are steps for generating mycelial mass in 24-well reuseable deep well plates, efficient lyophilization and disruption of the mycelium and genomic DNA extraction with 96-well glass fiber filter plates. The resulting DNA is consistently high molecular weight and is suitable for applications that require high quality DNA such as AFLP analysis and TILLING. A single operator easily can manage mycelium preparation and/or DNA extraction from 384 isolates in a single day and this approach might be useful for other fungi or fungi-like organisms that can be grown in liquid media.
journal_name
Mycologiajournal_title
Mycologiaauthors
Lamour K,Finley Ldoi
10.3852/mycologia.98.3.514subject
Has Abstractpub_date
2006-05-01 00:00:00pages
514-7issue
3eissn
0027-5514issn
1557-2536journal_volume
98pub_type
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