A strategy for recovering high quality genomic DNA from a large number of Phytophthora isolates.

Abstract:

:We present a strategy to recover high molecular weight genomic DNA from large numbers of isolates of Phytophthora. Included are steps for generating mycelial mass in 24-well reuseable deep well plates, efficient lyophilization and disruption of the mycelium and genomic DNA extraction with 96-well glass fiber filter plates. The resulting DNA is consistently high molecular weight and is suitable for applications that require high quality DNA such as AFLP analysis and TILLING. A single operator easily can manage mycelium preparation and/or DNA extraction from 384 isolates in a single day and this approach might be useful for other fungi or fungi-like organisms that can be grown in liquid media.

journal_name

Mycologia

journal_title

Mycologia

authors

Lamour K,Finley L

doi

10.3852/mycologia.98.3.514

subject

Has Abstract

pub_date

2006-05-01 00:00:00

pages

514-7

issue

3

eissn

0027-5514

issn

1557-2536

journal_volume

98

pub_type

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