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In vitro antioxidant, antiplatelet and anti-inflammatory activity of Carpobrotus rossii (pigface) extract.

Abstract：

ETHNOPHARMACOLOGICAL RELEVANCE:Carpobrotus rossii (CR) has a history of use as a food and therapeutic agent by Australian indigenous peoples and early European settlers and is believed to contain a number of pharmacologically active polyphenolic compounds. AIMS OF THE STUDY:Oxidation of low density lipoprotein (LDL), platelet aggregation, and inflammation contribute to the development and progression of atherosclerosis. The aim of the present study was to investigate the antioxidant, antiplatelet and anti-inflammatory activity of CR extract using human blood components. MATERIALS AND METHODS:An assay employing in vitro copper-induced oxidation of serum lipids was used to assess antioxidant activity of CR extract (and tannin, flavonoid and pre- and post-flavonoid fractions). The effects of CR extract on ADP- and collagen-induced platelet aggregation, and on basal (unstimulated) and lipopolysaccharide (LPS)- and phytohaemagglutinin A (PHA)-stimulated cytokine release from peripheral blood mononuclear cells (PBMC) were also investigated. RESULTS:CR extract increased the lag time of serum oxidation (maximum of ∼4-fold at 20μg/ml) in a concentration-dependent manner. The antioxidant activity resided only in the tannin and post-flavonoid fractions. CR had no effect on ADP-induced platelet aggregation, but significantly decreased collagen-induced platelet aggregation. LPS, but not PHA, significantly increased the release of IL-1β and TNF-α from PBMC. CR extract alone inhibited monocyte chemoattractant protein (MCP)-1 release and in the presence of LPS, inhibited IL-10, TNF-α and MCP-1 release compared to LPS alone. CONCLUSION:CR has significant in vitro antioxidant, antiplatelet and, potentially, anti-inflammatory activity.

journal_name

J Ethnopharmacol

journal_title

Journal of ethnopharmacology

authors

Geraghty DP,Ahuja KD,Pittaway J,Shing C,Jacobson GA,Jager N,Jurković S,Narkowicz C,Saunders CI,Ball M,Pinkard A,Vennavaram RR,Adams MJ

doi

10.1016/j.jep.2010.11.060

subject

Has Abstract

pub_date

2011-03-08 00:00:00

pages

97-103

issue

eissn

0378-8741

issn

1872-7573

pii

S0378-8741(10)00845-7

journal_volume

134

pub_type

杂志文章

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In vitro antioxidant, antiplatelet and anti-inflammatory activity of Carpobrotus rossii (pigface) extract.