Evaluation of virulence factor profiling in the characterization of veterinary Escherichia coli isolates.

Abstract:

:Escherichia coli has been used as an indicator organism for fecal contamination of water and other environments and is often a commensal organism in healthy animals, yet a number of strains can cause disease in young or immunocompromised animals. In this study, 281 E. coli isolates from bovine, porcine, chicken, canine, equine, feline, and other veterinary sources were analyzed by BOXA1R PCR and by virulence factor profiling of 35 factors to determine whether they had utility in identifying the animal source of the isolates. The results of BOXA1R PCR analysis demonstrated a high degree of diversity; less than half of the isolates fell into one of 27 clusters with at least three isolates (based on 90% similarity). Nearly 60% of these clusters contained isolates from more than one animal source. Conversely, the results of virulence factor profiling demonstrated clustering by animal source. Three clusters, named Bovine, Chicken, and Porcine, based on discriminant components analysis, were represented by 90% or more of the respective isolates. A fourth group, termed Companion, was the most diverse, containing at least 84% of isolates from canine, feline, equine, and other animal sources. Based on these results, it appears that virulence factor profiling may have utility, helping identify the likely animal host species sources of certain E. coli isolates.

journal_name

Appl Environ Microbiol

authors

David DE,Lynne AM,Han J,Foley SL

doi

10.1128/AEM.00726-10

subject

Has Abstract

pub_date

2010-11-01 00:00:00

pages

7509-13

issue

22

eissn

0099-2240

issn

1098-5336

pii

AEM.00726-10

journal_volume

76

pub_type

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