Abstract:
:The cytotoxic and genotoxic effects induced by alachlor and dichlorvos, two pesticides broadly used, were studied on RTG-2 fish cell line. As measure of cytotoxicity, neutral red assay was used to determine the cellular viability. Toxicity ranking based on IC(50) values found that alachlor was more cytotoxic than dichlorvos. DNA damage has been evaluated on RTG-2 cultures by means of an in vitro assay based on the ability of PicoGreen fluorochrome to interact preferentially with double-stranded DNA (dsDNA), and the results indicated that alachlor induced DNA strand breaks at concentrations above 1.52 μg/mL, equivalent to 1/50-EC(50(48)) , whereas exposures to dichlorvos induced DNA damage only at the maximal concentrations tested 25 μg/mL (1/10-EC(50(48)) ). These results confirm the suitability of this method for the screening of genotoxic effects of this type of aquatic pollutants, and we suggest their use in hazard assessment for environmental risk procedures.
journal_name
Environ Toxicoljournal_title
Environmental toxicologyauthors
Parra JM,Sánchez-Fortún S,Castaño Adoi
10.1002/tox.20637subject
Has Abstractpub_date
2012-03-01 00:00:00pages
238-43issue
4eissn
1520-4081issn
1522-7278journal_volume
27pub_type
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