Amino acid residues modulating the activities of staphylococcal glutamyl endopeptidases.

abstract：:DEAD-box proteins disrupt or remodel RNA and protein/RNA complexes at the expense of ATP. The catalytic core is composed of two flexibly connected RecA-like domains. The N-terminal domain contains most of the motifs involved in nucleotide binding and serves as a minimalistic model for helicase/nucleotide interactions....

journal_title：Biological chemistry

authors： Strohmeier J,Hertel I,Diederichsen U,Rudolph MG,Klostermeier D

更新日期：2011-04-01 00:00:00

abstract：:The enzymatic properties of glycosylphosphatidylinositol-specific phospholipase D (EC 3.1.4.50) were characterized using a 6,000-fold purified enzyme. This was obtained in 100 microg amounts from human serum with a recovery of 35%. Pure alkaline phosphatase containing one anchor moiety per molecule was used as substra...

journal_title：Biological chemistry

authors： Rhode H,Schulze M,Cumme GA,Göhlert A,Blume E,Bublitz R,Schilling K,Horn A

更新日期：2000-05-01 00:00:00

abstract：:Recent evidence suggests that epidermal growth factor receptor (EGFR)-dependent signaling contributes to liver cell proliferation, as well as to apoptotic liver cell death, and represents an important regulator of hepatic regeneration. This article summarizes recent findings on the molecular mechanisms involved in EGF...

journal_title：Biological chemistry

authors： Reinehr R,Häussinger D

更新日期：2009-10-01 00:00:00

abstract：:Transforming growth factor-β (TGFβ) signaling acts as suppressor and inducer of tumor progression during the early and late stages of cancer, respectively. Some miRNAs have shown a regulatory effect on TGFβ signaling and here, we have used a combination of bioinformatics and experimental tools to show that hsa-miR-559...

journal_title：Biological chemistry

authors： Abedini Bakhshmand E,Soltani BM

更新日期：2019-04-24 00:00:00

abstract：:We recently reported that human melanoma cells, but not benign melanocytes, aberrantly express kallikrein-related peptidase 7 (KLK7). Here, we show a KLK7 overexpression-mediated decrease of cell adhesion to extracellular matrix binding proteins, associated with downregulation of α5/β1/αv/β3 integrin expression. We al...

journal_title：Biological chemistry

authors： Haddada M,Draoui H,Deschamps L,Walker F,Delaunay T,Brattsand M,Magdolen V,Darmoul D

更新日期：2018-09-25 00:00:00

abstract：:The accumulation of cholesterol is a general feature of cancer tissue, and recent evidence suggests that cholesterol plays critical roles in the progression of cancers, including breast, prostate, and colorectal cancers. The dysregulation of metabolic pathways, including those involved in cholesterol biosynthesis, is ...

journal_title：Biological chemistry

authors： Murai T

更新日期：2015-01-01 00:00:00

abstract：:We have cloned from a chicken intestinal cDNA library Cmdr1, the first avian P-glycoprotein. Cmdr1 is 67% and 69% identical to proteins encoded by the human MDR1 and MDR2 genes, respectively. Functional expression of Cmdr1 in both mouse NIH 3T3 and yeast cells demonstrated that Cmdr1 represents the avian ortholog of h...

journal_title：Biological chemistry

authors： Edelmann HM,Duchek P,Rosenthal FE,Föger N,Glackin C,Kane SE,Kuchler K

更新日期：1999-02-01 00:00:00

abstract：:The infectivity and pathogenicity of influenza virus are primarily determined by host cellular trypsin-type processing proteases which cleave the viral membrane fusion glycoprotein hemagglutinin (HA). Therefore the distribution of the processing protease is a major determinant of the infectious organ tropism. The comm...

journal_title：Biological chemistry

authors： Yao D,Chen Y,Kuwajima M,Shiota M,Kido H

更新日期：2004-06-01 00:00:00

abstract：:The T-knot scaffold, a disulphide-reinforced structural motif shared by several proteins with very different biological functions, has been defined as 'a stretch of the protein chain which comprises two strands of a beta-sheet and three loops, knotted by two disulphides into the shape of the letter T'. In this communi...

journal_title：Biological chemistry

authors： Polticelli F,Pascarella S,Bordo D,Bolognesi M,Ascenzi P

更新日期：1999-10-01 00:00:00

abstract：:Abstract Hairpin ribozymes occur naturally only in the satellite RNAs of tobacco ringspot virus (TRsV), chicory yellow mottle virus (CYMoV) and arabis mosaic virus (ArMV). The catalytic centre of the predominantly studied sTRsV hairpin ribozyme, and of sArMV is organised around a four-way helical junction. We show her...

journal_title：Biological chemistry

authors： Bajaj P,Steger G,Hammann C

更新日期：2011-07-01 00:00:00

abstract：:Induction of apoptosis by selective BH3-mimetics is currently investigated as a novel strategy for cancer treatment. Here, we report that selective BH3-mimetics induce apoptosis in a variety of hematological malignancies. Apoptosis is accompanied by severe mitochondrial toxicities upstream of caspase activation. Speci...

journal_title：Biological chemistry

authors： Henz K,Al-Zebeeby A,Basoglu M,Fulda S,Cohen GM,Varadarajan S,Vogler M

更新日期：2019-01-28 00:00:00

abstract：:Protein cofactors often are the business ends of proteins, and are either synthesized inside cells or are taken up from the nutrition. A cofactor that strictly needs to be synthesized by cells is the iron-sulfur (Fe/S) cluster. This evolutionary ancient compound performs numerous biochemical functions including electr...

journal_title：Biological chemistry

authors： Lill R

更新日期：2020-05-26 00:00:00

abstract：:Based on studies presented here and other published experiments performed with surviving tissue preparations, with transfected cells and with cells that constitutively express the human angiotensin I converting enzyme ACE and B2 receptors, we concluded the following: ACE inhibitors and other endogenous peptides that r...

journal_title：Biological chemistry

authors： Erdös EG,Marcic BM

更新日期：2001-01-01 00:00:00

abstract：:Major vault protein (MVP) is the predominant member of a large cytosolic ribonucleoprotein particle, termed vault. We have previously shown that MVP derived from electric ray electric organ becomes phosphorylated by protein kinase C in vitro and by tyrosine kinase in vivo. Here we show that MVP from two mammalian cell...

journal_title：Biological chemistry

authors： Ehrnsperger C,Volknandt W

更新日期：2001-10-01 00:00:00

abstract：:The three-dimensional structure of pterin-4a-carbinolamine dehydratase (PCD) from Pseudomonas aeruginosa has been solved. Based on this we have investigated the roles of putative active center residues through functional replacement by site-directed mutagenesis. Three histidines, His73, His74 and His91, appear to be i...

journal_title：Biological chemistry

authors： Köster S,Stier G,Kubasch N,Curtius HC,Ghisla S

更新日期：1998-12-01 00:00:00

abstract：:Arginine deiminase (ADI) is a potential antitumor agent for the arginine deprivation treatment of L-arginine auxotrophic tumors. The optimum pH of ADI varies significantly, yet little is known about the origin of this variety. Here, Pseudomonas aeruginosa ADI (PaADI), an enzyme that functions only at acidic pH, was ut...

journal_title：Biological chemistry

authors： Ding H,Liu H,Yin Y,Ding Y,Jia Y,Chen Q,Zou G,Zheng Z

更新日期：2012-09-01 00:00:00

abstract：:Oxidative stress is a widespread phenomenon in the pathology of neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, and amyotrophic lateral sclerosis. Neuronal cell death due to oxidative stress may causally contribute to the pathogeneses of these diseases. Therefore, neuroprotective antioxida...

journal_title：Biological chemistry

authors： Moosmann B,Skutella T,Beyer K,Behl C

更新日期：2001-11-01 00:00:00

abstract：:There is increasing evidence that apoptosis and necrosis represent only two of several possible ways for cells to die. These two types of demise can occur simultaneously in tissues or cell cultures exposed to the same stimulus, and often local metabolic conditions and the intensity of the same initial insult decide th...

journal_title：Biological chemistry

authors： Nicotera P,Leist M,Single B,Volbracht C

更新日期：1999-09-01 00:00:00

abstract：:Protein transport, especially into different cellular compartments, is a highly coordinated and regulated process. The molecular machineries which carry out these transport processes are highly complex in structure, function, and regulation. In the case of chloroplasts, thousands of protein molecules have been estimat...

journal_title：Biological chemistry

authors： Sommer MS,Schleiff E

更新日期：2009-08-01 00:00:00

abstract：:Haptoglobin (Hpt) is known to capture circulating free hemoglobin (Hb) and bind apolipoprotein (Apo) A-I or E. Here, we report that Hb can be tightly bound by most of Hpt molecules (TB-Hpt, 80%), whereas loosely bound by a minor part of them (LB-Hpt, 20%). LB-Hpt amount was significantly increased (over 60%) in patien...

journal_title：Biological chemistry

authors： Spagnuolo MS,Cigliano L,Maresca B,Pugliese CR,Abrescia P

更新日期：2011-04-01 00:00:00

abstract：:The cellular adhesion molecule E-selectin is expressed on activated endothelial cells, and is involved in the process of adherence of blood cells to vessel endothelium in inflammatory events such as atherosclerosis. In a recent study we found a Ser128Arg mutation in the EGF domain as well as a Leu554Phe mutation in th...

journal_title：Biological chemistry

authors： Wenzel K,Stahn R,Speer A,Denner K,Gläser C,Affeldt M,Moobed M,Scheer A,Baumann G,Felix SB

更新日期：1999-06-01 00:00:00

abstract：:The 15 human kallikrein-related peptidases (KLKs) are clinically important biomarkers and therapeutic targets of interest in inflammation, cancer, and neurodegenerative disease. KLKs are secreted as inactive pro-forms (pro-KLKs) that are activated extracellularly by specific proteolytic release of their amino-terminal...

journal_title：Biological chemistry

authors： Yoon H,Blaber SI,Li W,Scarisbrick IA,Blaber M

更新日期：2013-01-01 00:00:00

abstract：:One of the control mechanisms of cathepsin B biosynthesis and trafficking operates through alternative splicing of pre-mRNA. An mRNA lacking exon 2 is more efficiently translated than that containing all exons, and may be responsible for elevated biosynthesis and enzyme routing to the extracellular space, with critica...

journal_title：Biological chemistry

authors： Baici A,Müntener K,Willimann A,Zwicky R

更新日期：2006-08-01 00:00:00

abstract：:The expression of the cysteine protease cathepsin B is increased in early stages of human breast cancer.To assess the potential role of cathepsin B in premalignant progression of breast epithelial cells, we employed a 3D reconstituted basement membrane overlay culture model of MCF10A human breast epithelial cells and ...

journal_title：Biological chemistry

authors： Mullins SR,Sameni M,Blum G,Bogyo M,Sloane BF,Moin K

更新日期：2012-12-01 00:00:00

abstract：:Molecular chaperones are well known as facilitators of protein folding and assembly. However, in recent years multiple chaperone-assisted degradation pathways have also emerged, including CAP (chaperone-assisted proteasomal degradation), CASA (chaperone-assisted selective autophagy), and CMA (chaperone-mediated autoph...

journal_title：Biological chemistry

authors： Kettern N,Dreiseidler M,Tawo R,Höhfeld J

更新日期：2010-05-01 00:00:00

abstract：:HB13 human myoblasts express physiological and biochemical markers associated with myoblast differentiation in non-human cell culture model systems. During differentiation, HB13 myoblasts also demonstrate fusion-related increases in cathepsin B activity and protein levels. These increases are associated with an increa...

journal_title：Biological chemistry

authors： Jane DT,Morvay LC,Koblinski J,Yan S,Saad FA,Sloane BF,Dufresne MJ

更新日期：2002-12-01 00:00:00

abstract：:Calpain is a calcium-activated non lysosomal neutral thiol protease (EC 3.4.22.17) present in a wide variety of eukaryotic cells. Calpain is usually present as an inactive form and is activated by calcium ions and phospholipids. The ability of calpain to alter, by limited proteolysis, the activity or function of numer...

journal_title：Biological chemistry

authors： Yajima Y,Kawashima S

更新日期：2002-05-01 00:00:00

abstract：:Abstract Few genes are known to be involved in renal cell carcinoma (RCC) development and progression. The cell-specific transcription factor hepatocyte nuclear factor 4 alpha (HNF4 alpha) is down-regulated in RCC and we have shown that HNF4 alpha inhibits cell proliferation in the embryonic kidney cell line HEK293. T...

journal_title：Biological chemistry

authors： Grigo K,Wirsing A,Lucas B,Klein-Hitpass L,Ryffel GU

更新日期：2008-02-01 00:00:00

abstract：:Fanconi anemia is a fatal, hereditary chromosome instability syndrome of early childhood with progressive pancytopenia and cancer-proneness. Hypersensitivity to alkylating agents points to DNA repair inefficiency. Excess reactive oxygen intermediates and hypersensitivity to oxygen, all features of Fanconi anemia cells...

journal_title：Biological chemistry

authors： Kontou M,Hirsch-Kauffmann M,Schweiger M

更新日期：2008-10-01 00:00:00

abstract：:We present a novel strategy, termed CISTEM, which allows direct in vivo screening of polypeptides displayed on the surface of E. coli cells by a combination of ligand-mediated protection and phage-mediated selection. The effectiveness of this new approach was demonstrated by displaying the T7.tag on the surface of E. ...

journal_title：Biological chemistry

authors： Camaj P,Hirsh AE,Schmidt W,Meinke A,von Gabain A

更新日期：2001-12-01 00:00:00