当前位置： SCI文献检索 > MARINE ENVIRONMENTAL RESEARCH期刊下所有文献 > Genetic polymorphism and its potential relation to environmental stress in five populations of the European flounder Platichthys flesus, along the French Atlantic coast.

Genetic polymorphism and its potential relation to environmental stress in five populations of the European flounder Platichthys flesus, along the French Atlantic coast.

Abstract：

:In this study, new DNA markers were explored for the flounder Platichthys flesus. cDNA and genomic sequences of the genes encoding the glyceraldehyde-3-phosphate-deshydrogenase (GAPDH), the cytosolic creatine kinase (CK), the prostaglandin D synthase (PGDS) and the betaine homocysteine methyltransferase (BHMT) were characterized. The tumour suppressor p53 gene structure was already described. A PCR-SSCP (Single Strand Conformation Polymorphism) analysis was finally conducted to study the genetic polymorphism of different populations of flounders collected along the French Atlantic coast. Four highly contaminated French estuaries (Seine, Vilaine, Loire and Gironde) were sampled and compared to a reference estuary (Ster) to explore possible selective effect of the environment on specific allelic frequencies. Our results showed that two loci p53 and PGDS, could be potential markers of chemical stress: p53A allele frequency increased in contaminated systems compared to the reference system. In the Vilaine estuary, PGDS polymorphism could be related to pesticide stress.

journal_name

Mar Environ Res

journal_title

Marine environmental research

authors

Marchand J,Evrard E,Guinand B,Cachot J,Quiniou L,Laroche J

doi

10.1016/j.marenvres.2010.05.002

subject

Has Abstract

pub_date

2010-08-01 00:00:00

pages

201-9

issue

2

eissn

0141-1136

issn

1879-0291

pii

S0141-1136(10)00069-3

journal_volume

70

pub_type

杂志文章

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