Measurement of DNA single-strand breaks in gill and hemolymph cells of mussels, Mytilus sp., collected on the French Atlantic Coast.

Abstract:

:DNA single-strand breaks were measured by the comet assay in both gill and hemolymph cells of mussels collected in 3 sampling areas of the French coast (Pointe du Castelli, Pen Bron and Saint-Nazaire Harbour). Whole mussel tissue samples were also collected for the chemical determination of PAH, PCB and heavy metal concentrations. In mussel, a higher level of DNA strand breaks was measured in gill than in hemolymph cells (p < 0.01). Despite a factor of contamination from 2 to 3 between sites, no difference in the extent of mussel DNA strand breaks was shown between sampling locations (p > 0.05), questioning the sensitivity of the assays used in biomonitoring studies.

journal_name

Mar Environ Res

authors

Akcha F,Tanguy A,Leday G,Pelluhet L,Budzinski H,Chiffoleau JF

doi

10.1016/j.marenvres.2004.03.090

keywords:

subject

Has Abstract

pub_date

2004-08-01 00:00:00

pages

753-6

issue

2-5

eissn

0141-1136

issn

1879-0291

pii

S0141-1136(04)00124-2

journal_volume

58

pub_type

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