当前位置： SCI文献检索 > NEUROPATHOLOGY AND APPLIED NEUROBIOLOGY期刊下所有文献 > Ex vivo study of dentate gyrus neurogenesis in human pharmacoresistant temporal lobe epilepsy.

Ex vivo study of dentate gyrus neurogenesis in human pharmacoresistant temporal lobe epilepsy.

Abstract：

AIMS:Neurogenesis in adult humans occurs in at least two areas of the brain, the subventricular zone of the telencephalon and the subgranular layer of the dentate gyrus in the hippocampal formation. We studied dentate gyrus subgranular layer neurogenesis in patients subjected to tailored antero-mesial temporal resection including amygdalohippocampectomy due to pharmacoresistant temporal lobe epilepsy (TLE) using the in vitro neurosphere assay. METHODS:Sixteen patients were enrolled in the study; mesial temporal sclerosis (MTS) was present in eight patients. Neurogenesis was investigated by ex vivo neurosphere expansion in the presence of mitogens (epidermal growth factor + basic fibroblast growth factor) and spontaneous differentiation after mitogen withdrawal. Growth factor synthesis was investigated by qRT-PCR in neurospheres. RESULTS:We demonstrate that in vitro proliferation of cells derived from dentate gyrus of TLE patients is dependent on disease duration. Moreover, the presence of MTS impairs proliferation. As long as in vitro proliferation occurs, neurogenesis is maintained, and cells expressing a mature neurone phenotype (TuJ1, MAP2, GAD) are spontaneously formed after mitogen withdrawal. Finally, formed neurospheres express mRNAs encoding for growth (vascular endothelial growth factor) as well as neurotrophic factors (brain-derived neurotrophic factor, ciliary neurotrophic factor, glial-derived neurotrophic factor, nerve growth factor). CONCLUSION:We demonstrated that residual neurogenesis in the subgranular layer of the dentate gyrus in TLE is dependent on diseases duration and absent in MTS.

journal_name

Neuropathol Appl Neurobiol

journal_title

Neuropathology and applied neurobiology

authors

Paradisi M,Fernández M,Del Vecchio G,Lizzo G,Marucci G,Giulioni M,Pozzati E,Antonelli T,Lanzoni G,Bagnara GP,Giardino L,Calzà L

doi

10.1111/j.1365-2990.2010.01102.x

subject

Has Abstract

pub_date

2010-10-01 00:00:00

pages

535-50

issue

6

eissn

0305-1846

issn

1365-2990

pii

NAN1102

journal_volume

36

pub_type

杂志文章

相关文献

NEUROPATHOLOGY AND APPLIED NEUROBIOLOGY文献大全
  • The Dorothy Russell Memorial Lecture. The molecular and cellular sequelae of experimental traumatic brain injury: pathogenetic mechanisms.

    abstract：:The mechanisms underlying secondary or delayed cell death following traumatic brain injury (TBI) are poorly understood. Recent evidence from experimental models of TBI suggest that diffuse and widespread neuronal damage and loss is progressive and prolonged for months to years after the initial insult in selectively v...

    journal_title：Neuropathology and applied neurobiology

    pub_type： 杂志文章,评审

    doi：10.1046/j.1365-2990.1998.00121.x

    authors： McIntosh TK,Saatman KE,Raghupathi R,Graham DI,Smith DH,Lee VM,Trojanowski JQ

    更新日期：1998-08-01 00:00:00

  • Distribution of tangles and threads in the cerebral cortex in progressive supranuclear palsy.

    abstract：:Recent studies have described silver- and tau-positive glia and threads in the degenerating lesions of progressive supranuclear palsy. In this study, Gallyas-Braak silver impregnation and several immunohistochemical techniques were employed to examine the distribution of tangles, abnormal glia and threads in the cereb...

    journal_title：Neuropathology and applied neurobiology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2990.1995.tb01066.x

    authors： Hanihara T,Amano N,Takahashi T,Nagatomo H,Yagashita S

    更新日期：1995-08-01 00:00:00

  • 14,15-epoxyeicosatrienoic acid promotes production of brain derived neurotrophic factor from astrocytes and exerts neuroprotective effects during ischaemic injury.

    abstract：AIMS:14,15-Epoxyeicosatrienoic acid (14,15-EET) is abundantly expressed in brain and exerts protective effects against ischaemia. 14,15-EET is hydrolysed by soluble epoxide hydrolase (sEH). sEH-/- mice show a higher level of 14,15-EET in the brain. Astrocytes play a pivotal role in neuronal survival under ischaemic con...

    journal_title：Neuropathology and applied neurobiology

    pub_type： 杂志文章

    doi：10.1111/nan.12291

    authors： Yuan L,Liu J,Dong R,Zhu J,Tao C,Zheng R,Zhu S

    更新日期：2016-12-01 00:00:00

  • Sclerosing meningioma: immunohistochemical analysis of five cases.

    abstract：:Sclerosing meningioma is a rare morphologic subtype of meningioma and may be mistaken for atypical or malignant meningioma and astrocytoma or schwannoma because of marked collagen deposits and a sparse population of cells with little resemblance to meningothelial cells. Authors describe the histopathologic and immunop...

    journal_title：Neuropathology and applied neurobiology

    pub_type： 杂志文章

    doi：10.1046/j.0305-1846.2003.00517.x

    authors： Kim NR,Im SH,Chung CK,Suh YL,Choe G,Chi JG

    更新日期：2004-04-01 00:00:00

  • Immunohistochemical expression of trk receptor proteins in focal cortical dysplasia with intractable epilepsy.

    abstract：:Focal cortical dysplasia (FCD), which is often associated with intractable epilepsy, is a form of abnormal structure of the cerebral cortex caused by a disorder of normal neocortical development. In such cerebral lesions obtained from four patients (two male, two female; average age 32.3 years at operation), the immun...

    journal_title：Neuropathology and applied neurobiology

    pub_type： 临床试验,杂志文章

    doi：10.1046/j.1365-2990.1999.00180.x

    authors： Nishio S,Morioka T,Hamada Y,Hisada K,Fukui M

    更新日期：1999-06-01 00:00:00

  • Expression of Thy-1 antigen in reactive endoneurial capillaries of the rat following peripheral nerve damage.

    abstract：:Immunohistochemical staining of Thy-1 antigen was studied in rat peripheral nerves shortly after a complete crush lesion and after an epineural depot injection of lysophosphatidylcholine-palmitate (lysoPC). The Thy-1 antigen was expressed in endoneurial capillaries after 20-30 h and was visible previous to alterations...

    journal_title：Neuropathology and applied neurobiology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2990.1988.tb00865.x

    authors： Beuche W

    更新日期：1988-01-01 00:00:00

  • Oligodendrocyte progenitors in the embryonic spinal cord express DM-20.

    abstract：:Oligodendrocyte progenitors, originating in the ventral ventricular zone of the embryonic rodent spinal cord, migrate and differentiate into the oligodendrocytes myelinating the future white matter. Transcripts for the dm-20 isoform of the proteolipid protein (plp) gene are detectable initially in cells of the ventral...

    journal_title：Neuropathology and applied neurobiology

    pub_type： 杂志文章

    doi：

    authors： Dickinson PJ,Fanarraga ML,Griffiths IR,Barrie JM,Kyriakides E,Montague P

    更新日期：1996-06-01 00:00:00

  • Steroid hormone receptors in pituitary adenomas: a biochemical, immunohistochemical and morphometric study on cryostat sections.

    abstract：:Oestrogen receptors and progesterone receptors were measured by an isoelectric focussing technique in cytosols from cryostat sections of eight human pituitary adenomas. Cryostat sections adjacent to the assayed sample were stained for anterior pituitary hormones using the peroxidase-antiperoxidase technique, and the c...

    journal_title：Neuropathology and applied neurobiology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2990.1986.tb00158.x

    authors： Ironside JW,Dangerfield VJ,Timperley WR,Underwood JC

    更新日期：1986-11-01 00:00:00

  • Aspergillosis of the nervous system. Report of two cases.

    abstract：:Two cases of Aspergillus infection of the nervous system are reported. In the first case it occurred in a healthy individual and presented as a space occupying lesion mimicking a tuberculous granuloma. In the second case it was an opportunistic infection in a patient with predisposing conditions and manifested as chro...

    journal_title：Neuropathology and applied neurobiology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2990.1983.tb00131.x

    authors： Diengdoh JV,Barnard RO,Thomas DG

    更新日期：1983-11-01 00:00:00

  • Cytoplasmic intermediate filaments in cultured glial cells.

    abstract：:Monolayer cultures of rat glial cells derived from 12 to 14 day fetal spinal cords and rat C6 glioma cells were examined for immunofluorescent reactivity with human or rabbit autoantibodies to intermediate filaments and with rabbit antibody to GFAP. The embryonic glial cells and C6 glima cells reacted identically with...

    journal_title：Neuropathology and applied neurobiology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2990.1980.tb00213.x

    authors： Sotelo J,Toh BH,Lolait SJ,Yildiz A,Osung O,Holborow EJ

    更新日期：1980-07-01 00:00:00

  • Review: Mechanistic target of rapamycin (mTOR) pathway, focal cortical dysplasia and epilepsy.

    abstract：:Over the last decade, there has been increasing evidence that hyperactivation of the mechanistic target of rapamycin (mTOR) pathway is a hallmark of malformations of cortical development such as focal cortical dysplasia (FCD) or hemimegalencephaly. The mTOR pathway governs protein and lipid synthesis, cell growth and ...

    journal_title：Neuropathology and applied neurobiology

    pub_type： 杂志文章,评审

    doi：10.1111/nan.12463

    authors： Marsan E,Baulac S

    更新日期：2018-02-01 00:00:00

  • Unusual development of polyoma virus in the brains of two patients with the acquired immune deficiency syndrome (AIDS).

    abstract：:Two HIV-positive male patients presented with a variety of symptoms including hemiparesis, unsteadiness, progressive loss of vision and poor memory. There were multiple non-enhancing lesions shown by CT scan in the white matter of the cerebral hemispheres. Specimens obtained by burr-hole biopsy showed the features of ...

    journal_title：Neuropathology and applied neurobiology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2990.1989.tb01242.x

    authors： Scaravilli F,Ellis DS,Tovey G,Harcourt-Webster JN,Guiloff RJ,Sinclair E

    更新日期：1989-09-01 00:00:00

  • Amyloid beta peptide causes chronic glial cell activation and neuro-degeneration after intravitreal injection.

    abstract：:We have previously demonstrated that amyloid beta (Abeta) peptide is acutely toxic to retinal neurones in vivo and that this toxicity is mediated by an indirect mechanism. We have now extended these studies to look at the chronic effect of intravitreal injection of Abeta peptides on retinal ganglion cells (RGC), the p...

    journal_title：Neuropathology and applied neurobiology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2990.2005.00666.x

    authors： Walsh DT,Bresciani L,Saunders D,Manca MF,Jen A,Gentleman SM,Jen LS

    更新日期：2005-10-01 00:00:00

  • The ethics of reusing archived tissue for research.

    abstract：:Pathologists have been establishing archives of human organs and tissue for research use for many years now. Controversy has arisen recently over these collections, particularly with regard to the right of patients or relatives to consent to removal and retention of tissue, genetic research using stored tissue samples...

    journal_title：Neuropathology and applied neurobiology

    pub_type： 杂志文章,评审

    doi：10.1046/j.1365-2990.2000.00276.x

    authors： Ashcroft R

    更新日期：2000-10-01 00:00:00

  • Neuronal and astrocytic responses involving the serotonergic system in human spongiform encephalopathies.

    abstract：:The relationships between the degree of cortical prion protein (PrP) deposition, tissue vacuolation and astrocytosis were studied in the frontal cortex of 27 cases of human spongiform encephalopathy, encompassing 13 cases of sporadic Creutzfeldt-Jakob disease (sCJD), four cases of familial CJD (fCJD) (one owing to E20...

    journal_title：Neuropathology and applied neurobiology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2990.2003.00486.x

    authors： Fraser E,McDonagh AM,Head M,Bishop M,Ironside JW,Mann DM

    更新日期：2003-10-01 00:00:00

  • Childhood spinal muscular atrophy induces alterations in contractile and regulatory protein isoform expressions.

    abstract：AIMS:Although modifications of the survival motor neurone gene are responsible for most spinal muscular atrophy (SMA) cases, the molecular pathophysiology and the muscular target proteins involved are still unknown. The aim of this study was to compare the expression of contractile and regulatory protein isoforms in qu...

    journal_title：Neuropathology and applied neurobiology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2990.2008.00950.x

    authors： Stevens L,Bastide B,Maurage CA,Dupont E,Montel V,Cieniewski-Bernard C,Cuisset JM,Vallée L,Mounier Y

    更新日期：2008-12-01 00:00:00

  • Heterozygous APOE Christchurch in familial Alzheimer's disease without mutations in other Mendelian genes.

    abstract：:We present the clinical and neuropathological findings of a patient with early onset Alzheimer's dementia (AD), heterozygous carrier of the rare Apolipoprotein E Christchurch (APOEch) variant. The patient did not harbor any pathogenic mutation in known Mendelian genes related to AD or other neurodegenerative disorders...

    journal_title：Neuropathology and applied neurobiology

    pub_type： 杂志文章

    doi：10.1111/nan.12670

    authors： Hernandez I,Gelpi E,Molina-Porcel L,Bernal S,Rodríguez-Santiago B,Dols-Icardo O,Ruiz A,Alcolea D,Boada M,Lleó A,Clarimón J

    更新日期：2020-10-23 00:00:00

  • Review: Role of developmental inflammation and blood-brain barrier dysfunction in neurodevelopmental and neurodegenerative diseases.

    abstract：:The causes of most neurological disorders are not fully understood. Inflammation and blood-brain barrier dysfunction appear to play major roles in the pathology of these diseases. Inflammatory insults that occur during brain development may have widespread effects later in life for a spectrum of neurological disorders...

    journal_title：Neuropathology and applied neurobiology

    pub_type： 杂志文章,评审

    doi：10.1111/j.1365-2990.2008.01005.x

    authors： Stolp HB,Dziegielewska KM

    更新日期：2009-04-01 00:00:00

  • Single dose of MDMA causes extensive decrement of serotoninergic fibre density without blockage of the fast axonal transport in Dark Agouti rat brain and spinal cord.

    abstract：:Prolonged neurotoxicity of the recreational drug, MDMA (3,4-methylenedioxymethamphetamine) on serotoninergic axon terminals has been suggested. The effect of a single (15 mg/kg) dose of intraperitoneally administered MDMA on serotoninergic fibre density, defined by tryptophan hydroxylase (TpH) and serotonin transporte...

    journal_title：Neuropathology and applied neurobiology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2990.2006.00790.x

    authors： Kovács GG,Andó RD,Adori C,Kirilly E,Benedek A,Palkovits M,Bagdy G

    更新日期：2007-04-01 00:00:00

  • Recruitment of neural precursor cells from circumventricular organs of patients with cerebral ischaemia.

    abstract：AIMS:Adult neurogenesis is well described in the subventricular zone of the lateral ventricle walls and in the subgranular zone of the hippocampal dentate gyrus. However, recent studies indicate that self-renewal of neural stem cells (NSCs) is not restricted to these niches, but that diverse areas of the adult brain ar...

    journal_title：Neuropathology and applied neurobiology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2990.2012.01301.x

    authors： Sanin V,Heeß C,Kretzschmar HA,Schüller U

    更新日期：2013-08-01 00:00:00

  • Increased levels of UCHL1 are a compensatory response to disrupted ubiquitin homeostasis in spinal muscular atrophy and do not represent a viable therapeutic target.

    abstract：AIM:Levels of ubiquitin carboxyl-terminal hydrolase L1 (UCHL1) are robustly increased in spinal muscular atrophy (SMA) patient fibroblasts and mouse models. We therefore wanted to establish whether changes in UCHL1 contribute directly to disease pathogenesis, and to assess whether pharmacological inhibition of UCHL1 re...

    journal_title：Neuropathology and applied neurobiology

    pub_type： 杂志文章

    doi：10.1111/nan.12168

    authors： Powis RA,Mutsaers CA,Wishart TM,Hunter G,Wirth B,Gillingwater TH

    更新日期：2014-12-01 00:00:00

  • Occludin expression in microvessels of neoplastic and non-neoplastic human brain.

    abstract：:The tight junction protein occludin 'glues' normal, adjacent brain microvessel endothelial cells together. Malignant brain tumours cause cerebral oedema because they have leaky endothelial tight junctions, which allow plasma fluid to enter the brain from the microvessel lumen. In order to identify molecular abnormalit...

    journal_title：Neuropathology and applied neurobiology

    pub_type： 杂志文章

    doi：10.1046/j.0305-1846.2001.00341.x

    authors： Papadopoulos MC,Saadoun S,Woodrow CJ,Davies DC,Costa-Martins P,Moss RF,Krishna S,Bell BA

    更新日期：2001-10-01 00:00:00

  • Histone deacetylases (HDACs) in frontotemporal lobar degeneration.

    abstract：AIMS:Frontotemporal lobar degeneration (FTLD) is clinically and pathologically heterogeneous. Although associated with variations in MAPT, GRN and C9ORF72, the pathogenesis of these, and of other nongenetic, forms of FTLD, remains unknown. Epigenetic factors such as histone regulation by histone deacetylases (HDAC) may...

    journal_title：Neuropathology and applied neurobiology

    pub_type： 历史文章,杂志文章

    doi：10.1111/nan.12153

    authors： Whitehouse A,Doherty K,Yeh HH,Robinson AC,Rollinson S,Pickering-Brown S,Snowden J,Thompson JC,Davidson YS,Mann DM

    更新日期：2015-02-01 00:00:00

  • Spinal cord pathology and viral burden in homosexuals and drug users with AIDS.

    abstract：:Unless treated with effective antiretroviral therapy many AIDS patients develop a characteristic vacuolar myelopathy of the spinal cord associated with moderate clinical disability. Opinion is divided as to whether vacuolar myelopathy is causally linked to HIV myelitis. To investigate this further, spinal cord patholo...

    journal_title：Neuropathology and applied neurobiology

    pub_type： 临床试验,杂志文章

    doi：10.1046/j.1365-2990.1999.00152.x

    authors： Shepherd EJ,Brettle RP,Liberski PP,Aguzzi A,Ironside JW,Simmonds P,Bell JE

    更新日期：1999-02-01 00:00:00

  • Loss of axonal contact causes down-regulation of the PLP gene in oligodendrocytes: evidence from partial lesions of the optic nerve.

    abstract：:We have examined the influence that axons may have on the expression of proteolipid protein (PLP), the major myelin protein of the CNS. Partial transections were made of the optic nerve of adult rats to produce approximately a 50% loss of axons. Twenty-eight days after lesioning, sections of the distal nerves were imm...

    journal_title：Neuropathology and applied neurobiology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2990.1991.tb00725.x

    authors： McPhilemy K,Griffiths IR,Mitchell LS,Kennedy PG

    更新日期：1991-08-01 00:00:00

  • The development of necrosis and apoptosis in glioma: experimental findings using spheroid culture systems.

    abstract：:Cell death in gliomas may occur either by apoptosis, or, in the case of high grade tumours, by necrosis, but questions remain as to the pathogenesis and relationship between these processes. The development of cell death was investigated in multicellular glioma spheroid cultures. Spheroids model the development of cel...

    journal_title：Neuropathology and applied neurobiology

    pub_type： 杂志文章

    doi：10.1046/j.0305-1846.2001.00319.x

    authors： Bell HS,Whittle IR,Walker M,Leaver HA,Wharton SB

    更新日期：2001-08-01 00:00:00

  • Lysosomal storage results in impaired survival but normal neurite outgrowth in dorsal root ganglion neurones from a mouse model of Sandhoff disease.

    abstract：:Sandhoff disease is a heritable lysosomal storage disease resulting from impaired degradation of GM2 ganglioside and related substrates. A mouse model of Sandhoff disease created by gene targeting displays progressive neurological manifestations, similar to patients with the disease. In the present in vivo and in vitr...

    journal_title：Neuropathology and applied neurobiology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2990.2002.00366.x

    authors： Sango K,Yamanaka S,Ajiki K,Tokashiki A,Watabe K

    更新日期：2002-02-01 00:00:00

  • Spongiform encephalopathy: a neurocytologist's viewpoint with a note on Alzheimer's disease.

    abstract：:Ultrastructural studies of spongiform encephalopathy (SE) reveal no very early pathological changes in kuru where membrane lamellation has been reported. This observation is challenged. In the later stages of SE, two main theories are examined--the spiroplasma theory and the prion (6 nm filament) theory. Neither are s...

    journal_title：Neuropathology and applied neurobiology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2990.1986.tb00047.x

    authors： Gray EG

    更新日期：1986-03-01 00:00:00

  • Experimental traction injuries of ventral spinal nerve roots. A scanning electron microscopic study.

    abstract：:The aims of this experimental scanning electron microscopic study were to identify the levels at which cervical ventral nerve roots, or the rootlets by which they are attached to the spinal cord, rupture under traction and to document the resulting damage. This information would provide the basis of a rationale for re...

    journal_title：Neuropathology and applied neurobiology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2990.1989.tb01254.x

    authors： Bristol DC,Fraher JP

    更新日期：1989-11-01 00:00:00

  • Differential insular cortex subregional vulnerability to α-synuclein pathology in Parkinson's disease and dementia with Lewy bodies.

    abstract：AIM:The insular cortex consists of a heterogenous cytoarchitecture and diverse connections and is thought to integrate autonomic, cognitive, emotional and interoceptive functions to guide behaviour. In Parkinson's disease (PD) and dementia with Lewy bodies (DLB), it reveals α-synuclein pathology in advanced stages. The...

    journal_title：Neuropathology and applied neurobiology

    pub_type： 杂志文章

    doi：10.1111/nan.12501

    authors： Fathy YY,Jonker AJ,Oudejans E,de Jong FJJ,van Dam AW,Rozemuller AJM,van de Berg WDJ

    更新日期：2019-04-01 00:00:00