Abstract:
:We have shown that cortical acetylcholine modulates the balance between excitation and inhibition evoked in layer 5 pyramidal neurons of rat visual cortex [Lucas-Meunier E, Monier C, Amar M, Baux G, Frégnac Y, Fossier P (2009) Cereb Cortex 19:2411-2427]. Our aim is now to establish a functional basis for the role of the different types of muscarinic receptors (MRs) on glutamate fibers and on GABAergic interneurons and to analyse their contribution to the modulation of excitation-inhibition balance in the rat visual cortex. To ascertain that there was a basis for our functional study, we first checked for the presence of the various MR subtypes by single cell RT-PCR and immunolabeling experiments. Then, recording the composite responses in layer 5 pyramidal neurons to layer 1-2 stimulation (which also recruits cholinergic fibers) in the presence of specific antagonists of the different types of MR allowed us to determine their modulatory role. We show that the specific blockade of the widely distributed M1R (with the mamba toxin, MT7) induced a significant increase in the excitatory conductance without modifying the inhibitory conductance, pointing to a localization of M1R on glutamatergic neurons where their activation would decrease the release of glutamate. From our functional results, M2/M4Rs appear to be located on glutamatergic neurons afferent to the recorded layer 5 pyramidal neuron and they decrease glutamate release. The extended distribution of M4Rs in the cortex compared to the restricted distribution of M2R (layers 3-5) is in favour of a major role as a modulator of M4R. The selective antagonist of M3Rs, 4-DAMP, decreased the inhibitory conductance, showing that activated M3Rs increase the release of GABA and thus are located on GABAergic interneurons. The activation of the different types of MRs located either on glutamatergic neurons or on GABAergic interneurons converges to reinforce the dominance of inhibitory inputs thus decreasing the excitability of layer 5 pyramidal neurons.
journal_name
Neurosciencejournal_title
Neuroscienceauthors
Amar M,Lucas-Meunier E,Baux G,Fossier Pdoi
10.1016/j.neuroscience.2010.06.019subject
Has Abstractpub_date
2010-09-15 00:00:00pages
1610-20issue
4eissn
0306-4522issn
1873-7544pii
S0306-4522(10)00866-3journal_volume
169pub_type
杂志文章相关文献
NEUROSCIENCE文献大全abstract::The noradrenergic nerve terminals and their vesicle populations have been subject to much ultrastructural research which has served to support and extend data from biochemical analysis and pharmacological-physiological experiments. Although a great deal of information has been collected, there are still many problems ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(83)90132-x
更新日期:1983-10-01 00:00:00
abstract::We tested a hypothesis that the classical relation between movement time and index of difficulty (ID) in quick pointing action (Fitts' Law) reflects processes at the level of motor planning. Healthy subjects stood on a force platform and performed quick and accurate hand movements into targets of different size locate...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2012.11.043
更新日期:2013-02-12 00:00:00
abstract::Deficits in forepaw adjusting steps in rats have been proposed as a non-drug-induced model of the akinesia associated with Parkinson's disease. The present study examined the relationship between contralateral forepaw adjusting steps and dopamine depletion after medial forebrain bundle lesions with 6-hydroxydopamine. ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(98)00217-6
更新日期:1999-01-01 00:00:00
abstract::In addition to triggering vasoconstriction of peripheral blood vessels, which led to its discovery as a circulating neurohormone 50 years ago, serotonin (5-hydroxytryptamine) acts as a neurotransmitter/ modulator in the central nervous system and regulates local cerebral blood flow and vascular permeability through di...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(98)00196-1
更新日期:1998-10-01 00:00:00
abstract::The non-human primate models of Parkinson's disease which have been developed using the neurotoxin MPTP (1-methyl-4-phenyl-1,2,3,6 tetrahydropyridine) have proven to be either unstable or variable, or to display only a limited subset of parkinsonian features. The present study examined a new two-stage lesion approach ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(93)90176-g
更新日期:1993-01-01 00:00:00
abstract::Neuronal nicotinic acetylcholine receptors are members of the ligand-gated ion channel superfamily composed of alpha and beta subunits with specific structural, functional and pharmacological properties. In this study we have used immunohistochemistry to investigate the presence of nicotinic acetylcholine receptor sub...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(02)00223-3
更新日期:2002-01-01 00:00:00
abstract::Recently, the reports that sensory nerves contribute to induction and development of peripheral inflammation have been accumulating. Although neuropeptides have been thought to participate in modulation of inflammation, we supposed the involvement of cytokines. Interleukin-12 (IL-12) is a key regulator of cell-mediate...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2004.07.035
更新日期:2004-01-01 00:00:00
abstract::Parkinson's disease (PD) is a progressive aging disorder that affects millions worldwide, thus, disease-modifying-therapies are urgently needed. PD pathology includes α-synuclein (aSyn) accumulation as synucleinopathy. Loss of GM1 gangliosides occurs in PD brain, which is modeled in GM2 synthase transgenic mice. GM2+/...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2019.05.029
更新日期:2019-07-15 00:00:00
abstract::The trigeminal ganglia (TG) subserving the head and the dorsal root ganglia (DRG) subserving the rest of the body are homologous handling sensory neurons. Differences exist, as a number of signaling substances cause headache but no pain in the rest of the body. To date, very few genes involved in this difference have ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2017.03.027
更新日期:2017-05-14 00:00:00
abstract::Throughout rat embryogenesis we analysed the expression patterns of the three mature transcripts generated from the two calcitonin gene-related peptide genes: calcitonin, alpha-calcitonin gene-related peptide, and beta-calcitonin gene-related peptide messenger RNAs. In addition, we examined in parallel the distributio...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(98)00730-1
更新日期:1999-01-01 00:00:00
abstract::Parkinson's disease (PD) is a motor disorder that involves death of dopaminergic neurons in the substantia nigra pars compacta. Parkin is an autosomal recessive gene that is mutated in early onset PD. We investigated the role of parkin and autophagic clearance in postmortem nigrostriatal tissues from 22 non-familial s...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2012.12.018
更新日期:2013-03-01 00:00:00
abstract::Endopeptidase-24.11, which is identical with the common acute lymphoblastic leukemia antigen (CALLA), is a cell surface zinc metalloprotease that has the ability to hydrolyse a variety of physiologically active peptides. Interest in this enzyme is based on the view that it may play a role in the regulation of peptide ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(92)90382-c
更新日期:1992-09-01 00:00:00
abstract::The novel multifunctional brain permeable iron, chelator M30 [5-(N-methyl-N-propargyaminomethyl)-8-hydroxyquinoline] was shown to possess neuroprotective activities in vitro and in vivo, against several insults applicable to various neurodegenerative diseases, such as Alzheimer's disease, Parkinson's disease, and amyo...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2011.03.040
更新日期:2011-08-25 00:00:00
abstract::The present study examined projections of GABAergic and cholinergic neurons from the basal forebrain and preoptic-anterior hypothalamus to the "intermediate" part of the mediodorsal nucleus of the thalamus. Retrograde transport from this region of the mediodorsal nucleus was investigated using horseradish peroxidase-c...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(97)00573-3
更新日期:1998-07-01 00:00:00
abstract::We here investigated the effects of neonatal lesions of the entorhinal cortex (EC) in rats on maze learning and on structural alterations of its main projection region, the hippocampus, as well as other regions with anatomical connections to the EC that are involved in maze learning. Since early brain damage is consid...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2008.03.017
更新日期:2008-06-02 00:00:00
abstract::Neuropeptide Y is the most abundant peptide in the mammalian central nervous system and exhibits a variety of potent neurobiological functions. In the present study, double immunolabelling histochemistry was performed, using previously characterized antibodies against neuropeptide Y and the neuropeptide Y Y1 receptor ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(98)00089-x
更新日期:1998-10-01 00:00:00
abstract::Exercise has been shown to impact brain plasticity and function by involving the action of brain-derived neurotrophic factor (BDNF); however, mechanisms involved are poorly understood. Two types of BDNF coexist in the brain, the precursor (proBDNF) and its mature product (mBDNF), which preferentially bind specific rec...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2011.06.032
更新日期:2011-09-29 00:00:00
abstract::Nitric oxide synthase is the biosynthetic enzyme for the free radical neurotransmitter nitric oxide. Using an affinity-purified antiserum, nitric oxide synthase was found to be localized to peripheral ocular nerve fibers, related cranial ganglia, and the retina of the rat. In the eye, nitric oxide synthase-like immuno...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(93)90393-t
更新日期:1993-05-01 00:00:00
abstract::Chronic stress affects brain areas involved in learning and emotional responses. These alterations have been related with the development of cognitive deficits in major depression. The aim of this study was to determine the effect of chronic immobilization stress on the auditory and visual mesencephalic regions in the...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2005.07.032
更新日期:2005-01-01 00:00:00
abstract::Acute stress is known to evoke a discrete pattern of c-fos expression in the brain. The work reported here shows that this pattern is modified in regionally specific ways following repeated stress, and that this can be correlated with changes in telemetered heart rate, core temperature and corticosterone output that o...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(94)00532-a
更新日期:1995-02-01 00:00:00
abstract::High resolution immunogold cytochemistry was used to investigate the subcellular distribution of protein kinase C gamma and delta in Purkinje cells of the rat cerebellum. Postembedding incubation with an antibody raised to a peptide sequence near the C-terminus of protein kinase C gamma resulted in strong labelling al...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(97)00305-9
更新日期:1998-02-01 00:00:00
abstract::GABA immunoreactivity was studied in the optic tectum of the frog, Rana esculenta, by postembedding immunohistochemical methods at the light and electron microscopic levels. Nearly one-third of the total population of tectal cells appeared to be GABA-immunoreactive. The proportion of stained neurons was highest in lay...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(91)90051-o
更新日期:1991-01-01 00:00:00
abstract::The mesolimbic dopaminergic system, originating from the ventral tegmental area (VTA) is implicated in the rewarding properties of ethanol. VTA dopaminergic neurons are under the tonic control of GABAergic innervations. Application of GABAergic agents changes ethanol consumption. However, it is unclear how acute ethan...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2008.01.040
更新日期:2008-04-22 00:00:00
abstract::Serotonin induces 'spontaneous' (non-electrically evoked) excitatory postsynaptic currents in layer V pyramidal neurons in the prefrontal cortex. This is likely due to a serotonin2A receptor-mediated focal release of glutamate onto apical dendrites. In addition, activation of the serotonin2A receptor selectively enhan...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(01)00170-1
更新日期:2001-01-01 00:00:00
abstract::Synaptotagmin (syt) I is a Ca(2+) sensor that has been thought to trigger all vesicle secretion with similar mechanisms. However, given the calcium and stimulation requirements of small clear, and large dense core vesicles, we hypothesized that syt I expression differentially regulates vesicle release. Therefore, in t...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2012.05.020
更新日期:2012-08-30 00:00:00
abstract::In the present study we have examined the topographic and temporal patterns of expression of the limbic system associated membrane protein by light and electron microscopic immunocytochemistry in organotypic cultures of the rat brain. The regional, cellular and subcellular distribution of staining in young cultures wa...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(89)90192-9
更新日期:1989-01-01 00:00:00
abstract::We have investigated the effect of intracranial injections of the amnestic anti-metabolite, 2-deoxygalactose, and antibodies to the neural cell adhesion molecule on retention of a one-trial passive avoidance task in chicks. Groups of chicks received bilateral intracranial injections of 10 mumol/hemisphere 2-deoxygalac...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(93)90519-l
更新日期:1993-07-01 00:00:00
abstract::Prolonged stress triggers neuroinflammation, which plays a significant role in the development of depression; however, stressed people do not always suffer from depression because of individual differences in stress vulnerability. Negative cognitive bias (NCB) toward pessimistic judgment often underlies depressive epi...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2020.07.023
更新日期:2020-09-01 00:00:00
abstract::In vitro cell cultures are an important tool for obtaining insights into cellular processes in an isolated system and a supplement to in vivo animal experiments. While primary dissociated cultures permit a single homogeneous cell population to be studied, there is a clear need to explore the function of brain cells in...
journal_title:Neuroscience
pub_type: 杂志文章,评审
doi:10.1016/j.neuroscience.2015.07.086
更新日期:2015-10-01 00:00:00
abstract::Similarities in cellular morphology, afferentation, efferentation, and neurotransmitter content between the internal and external parts of the pallidum and the substantia nigra pars reticulata have long been noted. Here we present evidence that the globus pallidus, entopedunucular nucleus and substantia nigra pars ret...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(90)90149-x
更新日期:1990-01-01 00:00:00