Abstract:
INTRODUCTION:Intraarticular administration of autologous conditioned serum (ACS) recently demonstrated some clinical effectiveness in treatment of osteoarthritis (OA). The current study aims to evaluate the in vitro effects of ACS on cartilage proteoglycan (PG) metabolism, its composition and the effects on synovial fluid (SF) cytokine levels following intraarticular ACS administration. METHODS:The effect of conditioned serum on PG metabolism of cultured OA cartilage explants was compared to unconditioned serum. The effect of serum conditioning on levels of interleukin-1beta (IL-1beta), IL-4, IL-6, IL-10, IL-13, interferon gamma (IFN-gamma), tumor necrosis factor alpha (TNF-alpha), osteoprotegerin (OPG), oncostatin M (OSM), interleukin-1 receptor (IL-1ra) and transforming growth factor beta (TGF-beta) were measured by multiplex ELISA. As TNF-alpha levels were found to be increased in conditioned serum, the effect of TNF-alpha inhibition by etanercept on PG metabolism was studied in cartilage explants cultured in the presence of conditioned serum. Furthermore, cytokine levels in SF were measured three days after intraarticular ACS injection in OA patients to verify their retention time in the joint space. RESULTS:PG metabolism was not different in the presence of conditioned serum compared to unconditioned serum. Levels of the anti-inflammatory cytokines IL-1ra, TGF-beta, IL-10 as well as of pro-inflammatory cytokines IL-1beta, IL-6, TNF-alpha and OSM were increased. IL-4, IL-13 and IFN-gamma levels remained similar, while OPG levels decreased. TNF-alpha inhibition did not influence PG metabolism in cartilage explant culture in the presence of conditioned serum. Although OPG levels were higher and TGF-beta levels were clearly lower in ACS than in SF, intraarticular ACS injection in OA patients did not result in significant changes in these cytokine levels. CONCLUSIONS:ACS for treatment of osteoarthritis contains increased levels of anti-inflammatory as well as pro-inflammatory cytokines, in particular TNF-alpha, but conditioned serum does not seem to have a net direct effect on cartilage metabolism, even upon inhibition of TNF-alpha. The fast intraarticular clearance of cytokines in the injected ACS may explain the limited effects found previously in vivo.
journal_name
Arthritis Res Therjournal_title
Arthritis research & therapyauthors
Rutgers M,Saris DB,Dhert WJ,Creemers LBdoi
10.1186/ar3050subject
Has Abstractpub_date
2010-01-01 00:00:00pages
R114issue
3eissn
1478-6354issn
1478-6362pii
ar3050journal_volume
12pub_type
杂志文章abstract:INTRODUCTION:Microchimeric male fetal cells (MFCs) have been associated with systemic lupus erythematosus, and published studies have further correlated MFC with lupus nephritis (LN). In the present study, we evaluated the frequency of MFC in the renal tissue of patients with LN. METHODS:Twenty-seven renal biopsies we...
journal_title:Arthritis research & therapy
pub_type: 杂志文章
doi:10.1186/s13075-015-0615-4
更新日期:2015-04-15 00:00:00
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journal_title:Arthritis research & therapy
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journal_title:Arthritis research & therapy
pub_type: 临床试验,杂志文章
doi:10.1186/ar3702
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journal_title:Arthritis research & therapy
pub_type: 杂志文章
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journal_title:Arthritis research & therapy
pub_type: 杂志文章,评审
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更新日期:2012-04-30 00:00:00
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journal_title:Arthritis research & therapy
pub_type: 杂志文章
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更新日期:2013-10-24 00:00:00
abstract::Autoantibodies targeting intracellular proteins involved in key processes are detected in patients with idiopathic inflammatory myopathies. These myositis-specific autoantibodies have been increasingly demonstrated to correlate with distinct clinical phenotypes within the myositis spectrum. This review highlights the ...
journal_title:Arthritis research & therapy
pub_type: 杂志文章,评审
doi:10.1186/ar3275
更新日期:2011-03-18 00:00:00
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journal_title:Arthritis research & therapy
pub_type: 杂志文章
doi:10.1186/ar3792
更新日期:2012-04-02 00:00:00
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journal_title:Arthritis research & therapy
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journal_title:Arthritis research & therapy
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更新日期:2009-01-01 00:00:00
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journal_title:Arthritis research & therapy
pub_type: 评论,社论
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更新日期:2011-01-01 00:00:00
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journal_title:Arthritis research & therapy
pub_type: 杂志文章,随机对照试验
doi:10.1186/ar4680
更新日期:2014-08-06 00:00:00
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journal_title:Arthritis research & therapy
pub_type: 杂志文章
doi:10.1186/ar2298
更新日期:2007-01-01 00:00:00
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journal_title:Arthritis research & therapy
pub_type: 杂志文章
doi:10.1186/ar4678
更新日期:2014-07-31 00:00:00
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journal_title:Arthritis research & therapy
pub_type: 评论,社论,评审
doi:10.1186/ar2839
更新日期:2009-01-01 00:00:00
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journal_title:Arthritis research & therapy
pub_type: 杂志文章
doi:10.1186/ar3033
更新日期:2010-01-01 00:00:00
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journal_title:Arthritis research & therapy
pub_type: 杂志文章
doi:10.1186/s13075-014-0500-6
更新日期:2014-12-05 00:00:00
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journal_title:Arthritis research & therapy
pub_type: 杂志文章
doi:10.1186/s13075-014-0411-6
更新日期:2014-01-01 00:00:00
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journal_title:Arthritis research & therapy
pub_type: 杂志文章
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doi:10.1186/ar4536
更新日期:2014-04-10 00:00:00
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journal_title:Arthritis research & therapy
pub_type: 杂志文章,随机对照试验
doi:10.1186/ar4439
更新日期:2014-01-16 00:00:00
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journal_title:Arthritis research & therapy
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更新日期:2013-01-01 00:00:00
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doi:10.1186/s13075-020-2122-5
更新日期:2020-03-04 00:00:00
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journal_title:Arthritis research & therapy
pub_type: 杂志文章
doi:10.1186/ar4065
更新日期:2012-10-18 00:00:00
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journal_title:Arthritis research & therapy
pub_type: 杂志文章
doi:10.1186/ar3881
更新日期:2012-06-14 00:00:00
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journal_title:Arthritis research & therapy
pub_type: 杂志文章
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更新日期:2018-06-08 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2011-01-01 00:00:00
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journal_title:Arthritis research & therapy
pub_type: 杂志文章
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更新日期:2010-01-01 00:00:00
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