Src-dependent TrkA transactivation is required for pituitary adenylate cyclase-activating polypeptide 38-mediated Rit activation and neuronal differentiation.

abstract：:To investigate the molecular interactions of synaptophysin I and vesicle-associated membrane protein 2 (VAMP2)/synaptobrevin II during exocytosis, we have used time-lapse videomicroscopy to measure fluorescence resonance energy transfer in live neurons. For this purpose, fluorescent protein variants fused to synaptoph...

journal_title：Molecular biology of the cell

authors： Pennuto M,Dunlap D,Contestabile A,Benfenati F,Valtorta F

更新日期：2002-08-01 00:00:00

abstract：:The c-myb proto-oncogene product (c-Myb) regulates proliferation and differentiation of hematopoietic cells. Recently we have shown that c-Myb is degraded in response to Wnt-1 stimulation via a pathway involving TAK1 (TGF-beta-activated kinase), HIPK2 (homeodomain-interacting protein kinase 2), and NLK (Nemo-like kina...

journal_title：Molecular biology of the cell

authors： Kurahashi T,Nomura T,Kanei-Ishii C,Shinkai Y,Ishii S

更新日期：2005-10-01 00:00:00

abstract：:The serotonin (5-hydroxytryptamine; 5-HT)(2C) receptor is a G protein-coupled receptor (GPCR) exclusively expressed in CNS that has been implicated in numerous brain disorders, including anxio-depressive states. Like many GPCRs, 5-HT(2C) receptors physically interact with a variety of intracellular proteins in additio...

journal_title：Molecular biology of the cell

authors： Labasque M,Reiter E,Becamel C,Bockaert J,Marin P

更新日期：2008-11-01 00:00:00

abstract：:Binding of echovirus 1 (EV1, a nonenveloped RNA virus) to the alpha2beta1 integrin on the cell surface is followed by endocytic internalization of the virus together with the receptor. Here, video-enhanced live microscopy revealed the rapid uptake of fluorescently labeled EV1 into mobile, intracellular structures, pos...

journal_title：Molecular biology of the cell

authors： Pietiäinen V,Marjomäki V,Upla P,Pelkmans L,Helenius A,Hyypiä T

更新日期：2004-11-01 00:00:00

abstract：:Three mitochondrial DNA-encoded proteins, Cox1, Cox2, and Cox3, comprise the core of the cytochrome c oxidase complex. Gene-specific translational activators ensure that these respiratory chain subunits are synthesized at the correct location and in stoichiometric ratios to prevent unassembled protein products from ge...

journal_title：Molecular biology of the cell

authors： Roloff GA,Henry MF

更新日期：2015-08-15 00:00:00

abstract：:We recently determined the nucleotide sequence of the gene encoding p28, a light chain of inner dynein arms of Chlamydomonas axonemes. Here, we show that p28 is the protein encoded by the IDA4 locus. p28, and the dynein heavy chains normally associated with it, are completely absent from the flagella and cell bodies o...

journal_title：Molecular biology of the cell

authors： LeDizet M,Piperno G

更新日期：1995-06-01 00:00:00

abstract：:Rab GTPases are localized to various intracellular compartments and are known to play important regulatory roles in membrane trafficking. Here, we report the subcellular distribution and function of Rab14. By immunofluorescence and immunoelectron microscopy, both endogenous as well as overexpressed Rab14 were localize...

journal_title：Molecular biology of the cell

authors： Junutula JR,De Maziére AM,Peden AA,Ervin KE,Advani RJ,van Dijk SM,Klumperman J,Scheller RH

更新日期：2004-05-01 00:00:00

abstract：:Calcium (Ca(2+)) and calmodulin (CaM) are required for progression of mammalian cells from quiescence into S phase. In multiple cell types, cyclosporin A causes a G(1) cell cycle arrest, implicating the serine/threonine phosphatase calcineurin as one Ca(2+)/CaM-dependent enzyme required for G(1) transit. Here, we show...

journal_title：Molecular biology of the cell

authors： Kahl CR,Means AR

更新日期：2004-04-01 00:00:00

abstract：:The CCAAT/enhancer binding protein delta (C/EBPdelta, CRP3, CELF, NF-IL6beta) regulates gene expression and plays functional roles in many tissues, such as in acute phase response to inflammatory stimuli, adipocyte differentiation, and mammary epithelial cell growth control. In this study, we examined the expression o...

journal_title：Molecular biology of the cell

authors： Wang JM,Tseng JT,Chang WC

更新日期：2005-07-01 00:00:00

abstract：:Focal adhesions are intricate protein complexes that facilitate cell attachment, migration, and cellular communication. Lasp-2 (LIM-nebulette), a member of the nebulin family of actin-binding proteins, is a newly identified component of these complexes. To gain further insights into the functional role of lasp-2, we i...

journal_title：Molecular biology of the cell

authors： Bliss KT,Chu M,Jones-Weinert CM,Gregorio CC

更新日期：2013-04-01 00:00:00

abstract：:Silica inhalation leads to the development of the chronic lung disease silicosis. Macrophages are killed by uptake of nonopsonized silica particles, and this is believed to play a critical role in the etiology of silicosis. However, the mechanism of nonopsonized-particle uptake is not well understood. We compared the ...

journal_title：Molecular biology of the cell

authors： Gilberti RM,Knecht DA

更新日期：2015-02-01 00:00:00

abstract：:Of the three structural maintenance of chromosomes (SMC) complexes, Smc5/6 remains the most poorly understood. Genetic studies have shown that Smc5/6 mutants are defective in homologous recombination (HR), and consistent with this, Smc5/6 is enriched at lesions. However, Smc5/6 is essential for viability, but HR is no...

journal_title：Molecular biology of the cell

authors： Tapia-Alveal C,O'Connell MJ

更新日期：2011-12-01 00:00:00

abstract：:In autosomal dominant polycystic kidney disease (ADPKD), cysts accumulate and progressively impair renal function. Mutations in PKD1 and PKD2 genes are causally linked to ADPKD, but how these mutations drive cell behaviors that underlie ADPKD pathogenesis is unknown. Human ADPKD cysts frequently express cadherin-8 (ca...

journal_title：Molecular biology of the cell

authors： Belmonte JM,Clendenon SG,Oliveira GM,Swat MH,Greene EV,Jeyaraman S,Glazier JA,Bacallao RL

更新日期：2016-11-07 00:00:00

abstract：:Disassembly of the epithelial apical junctional complex (AJC), composed of the tight junction (TJ) and adherens junction (AJ), is important for normal tissue remodeling and pathogen-induced disruption of epithelial barriers. Using a calcium depletion model in T84 epithelial cells, we previously found that disassembly ...

journal_title：Molecular biology of the cell

authors： Ivanov AI,McCall IC,Parkos CA,Nusrat A

更新日期：2004-06-01 00:00:00

abstract：:Cytochrome c release from mitochondria promotes apoptosome formation and caspase activation. The question as to whether mitochondrial permeabilization kills cells via a caspase-independent pathway when caspase activation is prevented is still open. Here we report that proneural cells of embryonic origin, when induced ...

journal_title：Molecular biology of the cell

authors： Ferraro E,Pulicati A,Cencioni MT,Cozzolino M,Navoni F,di Martino S,Nardacci R,Carrì MT,Cecconi F

更新日期：2008-08-01 00:00:00

abstract：:Secretory proteins are exported from the endoplasmic reticulum in COPII vesicles. SNARE proteins-core machinery for membrane fusion-are incorporated into COPII vesicles by direct interaction with Sec24. Here we report a novel mechanism for sorting of the ER-Golgi Q-SNAREs into COPII vesicles. Different mammalian Sec24...

journal_title：Molecular biology of the cell

authors： Adolf F,Rhiel M,Reckmann I,Wieland FT

更新日期：2016-09-01 00:00:00

abstract：:Cdc6 performs an essential role in the initiation of eukaryotic DNA replication by recruiting the minichromosome maintenance (MCM) complex onto DNA. Using immunodepletion/add-back experiments in Xenopus egg extracts, we have determined that both Walker A (ATP binding) and Walker B (ATP hydrolysis) motifs of Xenopus Cd...

journal_title：Molecular biology of the cell

authors： Frolova NS,Schek N,Tikhmyanova N,Coleman TR

更新日期：2002-04-01 00:00:00

abstract：:p38 alpha mitogen-activated protein (MAP) kinase is a broadly expressed signaling molecule that participates in the regulation of cellular responses to stress as well as in the control of proliferation and survival of many cell types. We have used cell lines derived from p38 alpha knockout mice to study the role of th...

journal_title：Molecular biology of the cell

authors： Porras A,Zuluaga S,Black E,Valladares A,Alvarez AM,Ambrosino C,Benito M,Nebreda AR

更新日期：2004-02-01 00:00:00

abstract：:Septins are conserved GTP-binding cytoskeletal proteins that polymerize into filaments by end-to-end joining of hetero-oligomeric complexes. In human cells, both hexamers and octamers exist, and crystallography studies predicted the order of the hexamers to be SEPT7-SEPT6-SEPT2-SEPT2-SEPT6-SEPT7, while octamers are th...

journal_title：Molecular biology of the cell

authors： Soroor F,Kim MS,Palander O,Balachandran Y,Collins RF,Benlekbir S,Rubinstein JL,Trimble WS

更新日期：2021-02-01 00:00:00

abstract：:More than 130 different mutations in the gap junction integral plasma membrane protein connexin32 (Cx32) have been linked to the human peripheral neuropathy X-linked Charcot-Marie-Tooth disease (CMTX). How these various mutants are processed by the cell and the mechanism(s) by which they cause CMTX are unknown. To add...

journal_title：Molecular biology of the cell

authors： VanSlyke JK,Deschenes SM,Musil LS

更新日期：2000-06-01 00:00:00

abstract：:The Saccharomyces cerevisiae a-factor receptor (Ste3p) requires its C-terminal cytoplasmic tail for endocytosis. Wild-type receptor is delivered to the cell surface via the secretory pathway but remains there only briefly before being internalized and delivered to the vacuole for degradation. Receptors lacking all or ...

journal_title：Molecular biology of the cell

authors： Givan SA,Sprague GF Jr

更新日期：1997-07-01 00:00:00

abstract：:Time-lapse fluorescence microscopy is an important tool for measuring in vivo gene dynamics in single cells. However, fluorescent proteins are limited by slow chromophore maturation times and the cellular autofluorescence or phototoxicity that arises from light excitation. An alternative is luciferase, an enzyme that ...

journal_title：Molecular biology of the cell

authors： Mazo-Vargas A,Park H,Aydin M,Buchler NE

更新日期：2014-11-05 00:00:00

abstract：:Damaged DNA-binding protein 2 (DDB2), a nuclear protein, participates in both nucleotide excision repair and mRNA transcription. The transcriptional regulatory function of DDB2 is significant in colon cancer, as it regulates metastasis. To characterize the mechanism by which DDB2 participates in transcription, we inve...

journal_title：Molecular biology of the cell

authors： Fantini D,Huang S,Asara JM,Bagchi S,Raychaudhuri P

更新日期：2017-01-01 00:00:00

abstract：:We used immunofluorescence to study the distribution and targeting of RNA polymerase (pol) III subunits and pol III transcription factors in the Xenopus laevis oocyte nucleus. Antibodies against several of these proteins stained Cajal bodies and approximately 90 specific sites on the lampbrush chromosomes. Some of the...

journal_title：Molecular biology of the cell

authors： Murphy C,Wang Z,Roeder RG,Gall JG

更新日期：2002-10-01 00:00:00

abstract：:A period of pairing between nonhomologous centromeres occurs early in meiosis in a diverse collection of organisms. This early, homology-independent, centromere pairing, referred to as centromere coupling in budding yeast, gives way to an alignment of homologous centromeres as homologues synapse later in meiotic proph...

journal_title：Molecular biology of the cell

authors： Chuong H,Dawson DS

更新日期：2010-06-01 00:00:00

abstract：:Polyubiquitination marks proteins for degradation by the 26S proteasome and is carried out by a cascade of enzymes that includes ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s). The anaphase-promoting complex or cyclosome (APC/C) comprises a multisubunit ubiquitin l...

journal_title：Molecular biology of the cell

authors： Leverson JD,Joazeiro CA,Page AM,Huang Hk,Hieter P,Hunter T

更新日期：2000-07-01 00:00:00

abstract：:In the yeast Saccharomyces cerevisiae, the Apg12p-Apg5p conjugating system is essential for autophagy. Apg7p is required for the conjugation reaction, because Apg12p is unable to form a conjugate with Apg5p in the apg7/cvt2 mutant. Apg7p shows a significant similarity to a ubiquitin-activating enzyme, Uba1p. In this a...

journal_title：Molecular biology of the cell

authors： Tanida I,Mizushima N,Kiyooka M,Ohsumi M,Ueno T,Ohsumi Y,Kominami E

更新日期：1999-05-01 00:00:00

abstract：:Hedgehog (Hh) is a secreted morphogen involved in both short- and long-range signaling necessary for tissue patterning during development. It is unclear how this dually lipidated protein is transported over a long range in the aqueous milieu of interstitial spaces. We previously showed that the long-range signaling of...

journal_title：Molecular biology of the cell

authors： Parchure A,Vyas N,Ferguson C,Parton RG,Mayor S

更新日期：2015-12-15 00:00:00

abstract：:Normal epithelial cells undergo apoptosis when they are denied contact with the extracellular matrix, in a process termed "anoikis." Conversely, malignant epithelial cells typically acquire anchorage independence, i.e., the capacity to survive and grow in the absence of matrix interaction. Here we asked the question w...

journal_title：Molecular biology of the cell

authors： Jost M,Huggett TM,Kari C,Rodeck U

更新日期：2001-05-01 00:00:00

abstract：:Golgi fragmentation is a common feature in multiple neurodegenerative diseases; however, the precise mechanism that causes fragmentation remains obscure. A potential link between Cdk5 and Golgi fragmentation in Alzheimer's disease (AD) was investigated in this study. Because Golgi is physiologically fragmented during ...

journal_title：Molecular biology of the cell

authors： Sun KH,de Pablo Y,Vincent F,Johnson EO,Chavers AK,Shah K

更新日期：2008-07-01 00:00:00