Abstract:
:Treatment of full-thickness damage to hyaline cartilage is hampered by the limited availability of autologous healthy cartilage and the lengthy, cost-prohibitive cell isolation and expansion steps associated with autologous cartilage implantation (ACI). Here we report a strategy for de novo engineering of ectopic autologous cartilage (EAC) within the subperiosteal space (in vivo bioreactor), through the mere introduction of a biocompatible gel that might promote hypoxia-mediated chondrogenesis, thereby effectively overcoming the aforementioned limitations. The EAC is obtained within 3 wk post injection of the gel, and can be press-fit into an osteochondral defect where it undergoes remodeling with good lateral and subchondral integration. The implanted EAC showed no calcification even after 9 mo and attained an average O'Driscoll score of 11 (versus 4 for controls). An "on demand" autologous source of autologous cartilage with remodeling capacity is expected to significantly impact the clinical options in repair of trauma to articular cartilage.
journal_name
Proc Natl Acad Sci U S Aauthors
Emans PJ,van Rhijn LW,Welting TJ,Cremers A,Wijnands N,Spaapen F,Voncken JW,Shastri VPdoi
10.1073/pnas.0907774107subject
Has Abstractpub_date
2010-02-23 00:00:00pages
3418-23issue
8eissn
0027-8424issn
1091-6490pii
0907774107journal_volume
107pub_type
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