Abstract:
:Primarily through in vitro studies, the Rho-family of small GTPases and their effector proteins have been implicated in mediating oncogenic properties of cancer cells. We sought to determine if pharmacological inhibition of the RhoA effector proteins known as Rho-kinases (ROCK) with the small molecule inhibitor Y-27632 could inhibit melanoma in vitro and in vivo. We demonstrate that Y-27632 treatment of a panel of melanoma cells alters cellular morphology leading to spindly cells with decreased lamellipodia and increased filopodia formation. Y-27632 treatment decreases invasion and alters cell survival of cultured melanoma cells. IP injection of Y-27632 in tumor-bearing mice resulted in a reduction in melanoma tumor volume compared to control treated mice. These findings suggest that ROCK inhibition can reduce melanoma tumorigenicity.
journal_name
Oncol Repjournal_title
Oncology reportsauthors
Routhier A,Astuccio M,Lahey D,Monfredo N,Johnson A,Callahan W,Partington A,Fellows K,Ouellette L,Zhidro S,Goodrow C,Smith A,Sullivan K,Simone P,Le L,Vezuli B,Zohni M,West E,Gleason D,Bryan Bsubject
Has Abstractpub_date
2010-03-01 00:00:00pages
861-7issue
3eissn
1021-335Xissn
1791-2431journal_volume
23pub_type
杂志文章相关文献
ONCOLOGY REPORTS文献大全abstract::Gene therapy mediated by murine leukemia virus (MLV)-based replicating retrovirus vector (RRV) was previously proven to be highly effective in tumor cell killing, resulting in significant suppression of tumor growth in vivo. Recently, we developed a different form of RRV which is derived from another retrovirus, gibbo...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2012.1789
更新日期:2012-07-01 00:00:00
abstract::In this study, we applied near-infrared fluorescent quantum dots (NIRF-QDs) for non-invasive in vivo and in situ imaging of head and neck squamous cell carcinoma (HNSCC). The U14 squamous cancer cell line with high expression of epidermal growth factor receptor (EGFR) was implanted subcutaneously into the head and nec...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2012.1705
更新日期:2012-06-01 00:00:00
abstract::Expression of neural differentiation-associated genes was examined by RT-PCR and macroarray analyses during neural differentiation of P19 embryonal carcinoma cells induced by cell aggregation and/or retinoic acid (RA) treatment. Results revealed that the neural genes examined could be classified into 4 groups based on...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2005-11-01 00:00:00
abstract::Non-small cell lung cancer (NSCLC) is the leading cause of cancer-related death worldwide, and only 15% of lung cancer patients live more than 5 years. microRNAs (miRNAs) are endogenously expressed non-coding RNAs, and dysregulation of miRNAs is a common feature in human cancers including lung cancer. In this study, w...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2611
更新日期:2013-10-01 00:00:00
abstract::The clinical significance of NK-like T CD3+/CD16+CD56+ cells in chronic lymphocytic leukemia (CLL) is still a subject of controversy. There are few previous descriptions that this cell population can be qualitatively or quantitatively deficient in CLL patients. In the present study we investigated the clinical value o...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or_00000924
更新日期:2010-09-01 00:00:00
abstract::Telomere-associated proteins function as survival factors in telomere maintenance, which are major contributors to radiosensitivity in human cancers. The aim of this study was to investigate the association of telomere-associated gene expression and radiation resistance in human larynx squamous carcinoma. The changes ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or_00000381
更新日期:2009-06-01 00:00:00
abstract::Soft tissue sarcomas are rare and aggressive tumors arising from connective tissues. Adjuvant radiotherapy is a commonly used treatment approach for the majority of sarcomas. We attempted to identify a gene signature that can predict radiosensitive patients who are most likely to have a better treatment response from ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2017.5999
更新日期:2017-11-01 00:00:00
abstract::Effective therapy for advanced hepatocellular carcinoma (HCC) is lacking. Conventional chemotherapy was judged to be ineffective. We previously demonstrated that the histone deacetylase inhibitor Trichostatin A (TSA) blocks growth of HCC cells in vitro. The anti-tumoral effect of a combination of more than 2 classes o...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2004-05-01 00:00:00
abstract::High mobility group box-B1 (HMGB1), an autophagy activator, is crucial in tumorigenesis. However, its extracellular role and signaling in gastric cancer remain unclear. Samples were collected from gastric cancer patients and healthy controls. Immunohistochemistry and immunocytochemistry were used to determine the loca...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.3782
更新日期:2015-04-01 00:00:00
abstract::Tanshinone IIA (Tan‑IIA; C19H18O3) is derived from Danshen (the roots of Salvia miltiorrhiza), and has been reported to possess anti‑inflammatory and antioxidant activities. Tan‑IIA can inhibit BxPC‑3 human pancreatic cancer cells in vitro through inducing endoplasmic reticulum stress and apoptosis via mitochondrial p...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2018.6670
更新日期:2018-11-01 00:00:00
abstract::Tetraspanins are transmembrane adaptor proteins involved in the regulation of various fundamental cellular processes. For a number of malignant diseases, the level of expression of members of the tetraspanin family was found to correlate with tumor cell invasiveness, ability to form metastases, and poor clinical outco...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2003-03-01 00:00:00
abstract::The aim of the present study was to evaluate the antitumor effects of the curcumin analogue GL63 on radioresistant nasopharyngeal carcinoma (NPC) CNE2R cells and parental CNE2 cells. The cell viability and proliferation of NPC cells were detected by MTT assay and colony formation assay. The suppressive effect on tumor...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2457
更新日期:2013-07-01 00:00:00
abstract::Antimycin A (AMA) inhibits the mitochondrial electron transport between cytochromes b and c. However, the relationship between AMA and lung cancer cells is poorly understood. In this study, we investigated the involvement of reactive oxygen species (ROS) and glutathione (GSH) in AMA-treated lung cancer Calu-6 cell dea...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2009-01-01 00:00:00
abstract::Frequent frameshift mutations in the DNA mismatch repair genes hMSH3, and hMSH6, have been reported in colorectal and endometrial cancers with microsatellite instability, however, it is unclear whether they are similarly altered in ovarian endometrioid carcinoma. In this study, we examined frequency of frameshift muta...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2004-12-01 00:00:00
abstract::The expression of the DNA mismatch repair proteins hMSH2 and hMLH1 and p21(waf1) the cyclin G1 inhibitor, may determine response of adult cancers to anti-cancer drugs, that include alkylating agents and platinum-based drugs. The role of DNA mismatch repair proteins (hMSH2 and hMLH1) and p21(waf1) in pediatric tumor re...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.8.5.965
更新日期:2001-09-01 00:00:00
abstract::Correlation between inhibition of cell motility and anti-invasive activity by the water extract of HangAmDan-B (HAD-B), a crude extract of eight Korean medicinal animals and plants, in NCI-H460 human non-small cell lung cancer (NSCLC) cells was investigated. Within the concentration...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2011.1440
更新日期:2011-12-01 00:00:00
abstract::Normal breast development is controlled by a balance between cell proliferation and apoptosis. The balance between the two parameters is crucial for determining the growth or regression of breast tumours in response to therapies and treatments. Therefore, it is necessary to understand the role of apoptosis in tumour p...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2008-09-01 00:00:00
abstract::The purpose of this study was to examine the effects of irradiation by 125I seeds in human lung cancer xenograft model and to determine the underlying mechanisms involved, with a focus on angiogenesis. A group of 40 mice bearing A549 lung adenocarcinoma xenografts was randomly separated into 4 groups: control group (n...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2017.5521
更新日期:2017-05-01 00:00:00
abstract::This study was aimed to obtain additional information on the activity of a cyclized 9-amino acid peptide (cP) containing the active site of alpha fetoprotein, which inhibits the estrogen-stimulated proliferation of tumor cells in culture and of xenografts in immunodeficient mice. Breast cancer cells cultured in the pr...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2008-01-01 00:00:00
abstract::The high affinity nerve growth factor (NGF) NGF receptor, p75NTR, is a member of the tumor necrosis factor (TNF) receptor superfamily that shares a conserved intracellular death domain capable of inducing apoptosis and suppressing growth in prostate epithelial cells. Expression of this receptor is lost as prostate can...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2016.4832
更新日期:2016-07-01 00:00:00
abstract::MicroRNAs (miRNAs/miRs) in exosomes play crucial roles in the onset, progression and metastasis of cancer by regulating the stability of target mRNAs or by inhibiting translation. In the present study, differentially expressed miRNAs were identified in exosomes of 27 breast cancer patients and 3 healthy controls using...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2019.7401
更新日期:2020-01-01 00:00:00
abstract::Resistance to docetaxel, a chemotherapy drug for breast cancer (BC) treatment, occurs in ~50% of patients, and the underlying molecular mechanisms of drug resistance are not fully understood. Gene regulation through miR-141 has been proven to play an important role in cancer drug resistance. The present study investig...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.3866
更新日期:2015-05-01 00:00:00
abstract::Ubiquitin specific protease 22 (USP22) is an oncogene that is upregulated in many cancer types, and aberrant expression of USP22 correlates with clinical outcome. However, its potential functional impact in epithelial ovarian cancer (EOC) has not been determined. Here, we report that USP22 was upregulated in EOC speci...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2014.3580
更新日期:2015-01-01 00:00:00
abstract::Lung cancer is the leading cause of cancer-related mortality worldwide. microRNAs (miRNAs) are small post-transcriptional regulatory non-coding RNAs that function as oncogenes or tumor suppressors in human cancers. Emerging evidence reveals that deregulation of miRNAs contributes to the progression of human lung cance...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2014.3637
更新日期:2015-02-01 00:00:00
abstract::High-density single nucleotide polymorphism (SNP) array is a recently introduced technology that genotypes more than 10,000 human SNPs on a single array. It has been shown that SNP arrays can be used to determine not only SNP genotype calls, but also DNA copy number (DCN) aberrations, which are common in solid tumors....
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.15.4.1057
更新日期:2006-01-01 00:00:00
abstract::The current study characterized peripheral blood mononuclear cells (PBMC) obtained from leukapheresis products of patients with non-small cell lung cancer (NSCLC) for cytokine release, the ability to incorporate tritiated thymidine following stimulation using PHA as well as the levels of both CD4 and CD8 regulatory T ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or_00000588
更新日期:2009-12-01 00:00:00
abstract::In the present study, a novel signaling pathway of microRNA-141 (miR-141)/fused in sarcoma (FUS) was investigated in neuroblastoma (NB). Gene expression of miR-141 was evaluated in 6 NB cell lines. IMR-32 and SH-SY5Y cells were transduced with the miR-141 mimic lentivirus. The effects of miR-141 upregulation on cell p...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2016.4640
更新日期:2016-05-01 00:00:00
abstract::Gastric cancer (GC) is one of the most common causes of cancer‑related mortality worldwide. Despite remarkable progress in the diagnosis and treatment of GC, a large number of cases are diagnosed as advanced GC, and treatment failure occurs. Emerging evidence has shown that non‑coding RNAs (ncRNAs), especially microRN...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2020.7705
更新日期:2020-10-01 00:00:00
abstract::Glucocorticoids such as dexamethasone are widely used as comedication in the treatment of head and neck cancer, e.g., to improve appetite and decrease weight loss and fatigue in patients with advanced disease or as antiallergic and antiemetic prophylaxis during anti-EGFR therapy. However, the literature suggests that ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or_00000421
更新日期:2009-07-01 00:00:00
abstract::In the present study, to evaluate the role of Ku80 in thyroid carcinoma (TC), 86 thyroid tissue samples from patients with a spectrum of thyroid disorders were examined for protein levels of Ku80, nuclear factor‑κB (NF‑κB) and RET/TC by immunohistochemistry. Furthermore, in TC cells, Ku80 mRNA was detected by reverse ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2019.7348
更新日期:2019-12-01 00:00:00