当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > Illuminating cell-cycle progression in the developing zebrafish embryo.

Illuminating cell-cycle progression in the developing zebrafish embryo.

Abstract：

:By exploiting the cell-cycle-dependent proteolysis of two ubiquitination oscillators, human Cdt1 and geminin, which are the direct substrates of SCF(Skp2) and APC(Cdh1) complexes, respectively, Fucci technique labels mammalian cell nuclei in G(1) and S/G(2)/M phases with different colors. Transgenic mice expressing these G(1) and S/G(2)/M markers offer a powerful means to investigate the coordination of the cell cycle with morphogenetic processes. We attempted to introduce these markers into zebrafish embryos to take advantage of their favorable optical properties. However, although the fundamental mechanisms for cell-cycle control appear to be well conserved among species, the G(1) marker based on the SCF(Skp2)-mediated degradation of human Cdt1 did not work in fish cells, probably because the marker was not ubiquitinated properly by a fish E3 ligase complex. We describe here the generation of a Fucci derivative using zebrafish homologs of Cdt1 and geminin, which provides sweeping views of cell proliferation in whole fish embryos. Remarkably, we discovered two anterior-to-posterior waves of cell-cycle transitions, G(1)/S and M/G(1), in the differentiating notochord. Our study demonstrates the effectiveness of using the Cul4(Ddb1)-mediated Cdt1 degradation pathway common to all metazoans for the development of a G(1) marker that works in the nonmammalian animal model.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Sugiyama M,Sakaue-Sawano A,Iimura T,Fukami K,Kitaguchi T,Kawakami K,Okamoto H,Higashijima S,Miyawaki A

doi

10.1073/pnas.0906464106

subject

Has Abstract

pub_date

2009-12-08 00:00:00

pages

20812-7

issue

49

eissn

0027-8424

issn

1091-6490

pii

0906464106

journal_volume

106

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • Immunogenic (tum-) variants of mouse tumor P815: cloning of the gene of tum- antigen P91A and identification of the tum- mutation.

    abstract：:Mutagen treatment of mouse P815 tumor cells produces tum- variants that are rejected by syngeneic mice because these variants express new surface antigens. These "tum- antigens" are recognized by cytolytic T lymphocytes but induce no detectable antibody response. Transfection of P815 cell line P1.HTR with DNA of tum- ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.85.7.2274

    authors： De Plaen E,Lurquin C,Van Pel A,Mariamé B,Szikora JP,Wölfel T,Sibille C,Chomez P,Boon T

    更新日期：1988-04-01 00:00:00

  • Defective CD95/APO-1/Fas signal complex formation in the human autoimmune lymphoproliferative syndrome, type Ia.

    abstract：:Heterozygous mutations in the CD95 (APO-1/Fas) receptor occur in most individuals with autoimmune lymphoproliferative syndrome (ALPS) and dominantly interfere with apoptosis by an unknown mechanism. We show that local or global alterations in the structure of the cytoplasmic death domain from nine independent ALPS CD9...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.96.8.4552

    authors： Martin DA,Zheng L,Siegel RM,Huang B,Fisher GH,Wang J,Jackson CE,Puck JM,Dale J,Straus SE,Peter ME,Krammer PH,Fesik S,Lenardo MJ

    更新日期：1999-04-13 00:00:00

  • Weighting of experimental evidence in macromolecular structure determination.

    abstract：:The determination of macromolecular structures requires weighting of experimental evidence relative to prior physical information. Although it can critically affect the quality of the calculated structures, experimental data are routinely weighted on an empirical basis. At present, cross-validation is the most rigorou...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0506412103

    authors： Habeck M,Rieping W,Nilges M

    更新日期：2006-02-07 00:00:00

  • A cation binding motif stabilizes the compound I radical of cytochrome c peroxidase.

    abstract：:Cytochrome c peroxidase reacts with peroxide to form compound I, which contains an oxyferryl heme and an indolyl radical at Trp-191. The indolyl free radical has a half-life of several hours at room temperature, and this remarkable stability is essential for the catalytic function of cytochrome c peroxidase. To probe ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.91.23.11118

    authors： Miller MA,Han GW,Kraut J

    更新日期：1994-11-08 00:00:00

  • Enhancing the antitumor efficacy of a cell-surface death ligand by covalent membrane display.

    abstract：:TNF superfamily death ligands are expressed on the surface of immune cells and can trigger apoptosis in susceptible cancer cells by engaging cognate death receptors. A recombinant soluble protein comprising the ectodomain of Apo2 ligand/TNF-related apoptosis-inducing ligand (Apo2L/TRAIL) has shown remarkable preclinic...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1418962112

    authors： Nair PM,Flores H,Gogineni A,Marsters S,Lawrence DA,Kelley RF,Ngu H,Sagolla M,Komuves L,Bourgon R,Settleman J,Ashkenazi A

    更新日期：2015-05-05 00:00:00

  • Ablation of SLP-76 signaling after T cell priming generates memory CD4 T cells impaired in steady-state and cytokine-driven homeostasis.

    abstract：:The intracellular signaling mechanisms regulating the generation and long-term persistence of memory T cells in vivo remain unclear. In this study, we used mouse models with conditional deletion of the key T cell receptor (TCR)-coupled adaptor molecule SH2-domain-containing phosphoprotein of 76 kDa (SLP-76), to analyz...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0908126107

    authors： Bushar ND,Corbo E,Schmidt M,Maltzman JS,Farber DL

    更新日期：2010-01-12 00:00:00

  • Innate immune receptor NOD2 mediates LGR5+ intestinal stem cell protection against ROS cytotoxicity via mitophagy stimulation.

    abstract：:The nucleotide-binding oligomerization domain-containing protein 2 (NOD2) agonist muramyl dipeptide (MDP), a peptidoglycan motif common to all bacteria, supports leucine-rich repeat-containing G protein-coupled receptor 5 (LGR5)+ intestinal stem cell (ISC) survival through NOD2 activation upon an otherwise lethal oxid...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1902788117

    authors： Levy A,Stedman A,Deutsch E,Donnadieu F,Virgin HW,Sansonetti PJ,Nigro G

    更新日期：2020-01-28 00:00:00

  • Functional interaction of erythropoietin and stem cell factor receptors is essential for erythroid colony formation.

    abstract：:Production of mature erythrocytes requires multiple growth factors, but we do not know how their actions are coordinated. Here we show that erythroid progenitors from erythropoietin receptor (Epo-R)-/- fetal livers, infected in vitro with a retrovirus expressing the wild-type Epo-R, require addition of both Epo and st...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.94.5.1806

    authors： Wu H,Klingmüller U,Acurio A,Hsiao JG,Lodish HF

    更新日期：1997-03-04 00:00:00

  • Role of an RNA cleavage/poly(A) addition site in the production of membrane-bound and secreted IgM mRNA.

    abstract：:The switch from membrane-bound to secreted IgM is accomplished by producing alternative forms of mRNA from a single mu heavy-chain gene. This process might be controlled at any of three steps--transcription termination, RNA splicing, or RNA cleavage/poly(A) addition. To distinguish between these possibilities, we have...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.82.24.8658

    authors： Danner D,Leder P

    更新日期：1985-12-01 00:00:00

  • Quantitative determination of ribosome nascent chain stability.

    abstract：:Accurate protein folding is essential for proper cellular and organismal function. In the cell, protein folding is carefully regulated; changes in folding homeostasis (proteostasis) can disrupt many cellular processes and have been implicated in various neurodegenerative diseases and other pathologies. For many protei...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1610272113

    authors： Samelson AJ,Jensen MK,Soto RA,Cate JH,Marqusee S

    更新日期：2016-11-22 00:00:00

  • Plasminogen activator inhibitor type 1 gene is located at region q21.3-q22 of chromosome 7 and genetically linked with cystic fibrosis.

    abstract：:The regional chromosomal location of the human gene for plasminogen activator inhibitor type 1 (PAI1) was determined by three independent methods of gene mapping. PAI1 was localized first to 7cen-q32 and then to 7q21.3-q22 by Southern blot hybridization analysis of a panel of human and mouse somatic cell hybrids with ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.84.23.8548

    authors： Klinger KW,Winqvist R,Riccio A,Andreasen PA,Sartorio R,Nielsen LS,Stuart N,Stanislovitis P,Watkins P,Douglas R

    更新日期：1987-12-01 00:00:00

  • Architected cellular ceramics with tailored stiffness via direct foam writing.

    abstract：:Hierarchical cellular structures are ubiquitous in nature because of their low-density, high-specific properties, and multifunctionality. Inspired by these systems, we created lightweight ceramic architectures composed of closed-cell porous struts patterned in the form of hexagonal and triangular honeycombs by direct ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1616769114

    authors： Muth JT,Dixon PG,Woish L,Gibson LJ,Lewis JA

    更新日期：2017-02-21 00:00:00

  • Multiple gamma-crystallins of the mouse lens: fractionation of mRNAs by cDNA cloning.

    abstract：:cDNAs made from polyadenylylated RNAs of the mouse lens were cloned by the G.C tailing procedure in the bacterial plasmid pBR322. Four recombinant DNAs containing gamma-crystallin sequences were identified by hybrid selection and translation. Sequence analysis of the in vivo-labeled gamma-crystallin polypeptides that ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.79.9.2783

    authors： Shinohara T,Robinson EA,Appella E,Piatigorsky J

    更新日期：1982-05-01 00:00:00

  • N-cadherin-dependent neuron-neuron interaction is required for the maintenance of activity-induced dendrite growth.

    abstract：:Formation of neural circuits depends on stable contacts between neuronal processes, mediated by interaction of cell adhesion molecules, including N-cadherin. In the present study, we found that activity-dependent dendrite arborization specifically requires N-cadherin-mediated extracellular neuron-neuron interaction, b...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1003480107

    authors： Tan ZJ,Peng Y,Song HL,Zheng JJ,Yu X

    更新日期：2010-05-25 00:00:00

  • Control of ColE1 DNA replication: the rop gene product negatively affects transcription from the replication primer promoter.

    abstract：:A 600-base-pair region essential for ColE1 and pMBl plasmid replication contains two promoters responsible for the synthesis of two RNA molecules central to copy number control. One promoter directs synthesis of the primer RNA precursor. The second promoter directs the synthesis of a small RNA molecule, RNAl, which ac...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.79.20.6313

    authors： Cesareni G,Muesing MA,Polisky B

    更新日期：1982-10-01 00:00:00

  • Visualizing the dynamics of T cell activation: intracellular adhesion molecule 1 migrates rapidly to the T cell/B cell interface and acts to sustain calcium levels.

    abstract：:T cell recognition typically involves both the engagement of a specific T cell receptor with a peptide/major histocompatibility complex (MHC) and a number of accessory interactions. One of the most important interactions is between the integrin lymphocyte function-associated antigen 1 (LFA-1) on the T cell and intrace...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.95.11.6302

    authors： Wülfing C,Sjaastad MD,Davis MM

    更新日期：1998-05-26 00:00:00

  • The axonal attractant Netrin-1 is an angiogenic factor.

    abstract：:Blood vessels and nerves often follow parallel trajectories, suggesting that distal targets use common cues that induce vascularization and innervation. Netrins are secreted by the floor plate and attract commissural axons toward the midline of the neural tube. Here, we show that Netrin-1 is also a potent vascular mit...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0405984101

    authors： Park KW,Crouse D,Lee M,Karnik SK,Sorensen LK,Murphy KJ,Kuo CJ,Li DY

    更新日期：2004-11-16 00:00:00

  • Fixation and fate of C and N in the cyanobacterium Trichodesmium using nanometer-scale secondary ion mass spectrometry.

    abstract：:The marine cyanobacterium Trichodesmium is ubiquitous in tropical and subtropical seas and is an important contributor to global N and C cycling. We sought to characterize metabolic uptake patterns in individual Trichodesmium IMS-101 cells by quantitatively imaging (13)C and (15)N uptake with high-resolution secondary...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0810547106

    authors： Finzi-Hart JA,Pett-Ridge J,Weber PK,Popa R,Fallon SJ,Gunderson T,Hutcheon ID,Nealson KH,Capone DG

    更新日期：2009-04-14 00:00:00

  • Identification of alterations in DNA copy number in host stromal cells during tumor progression.

    abstract：:The interactions between cancer cells and the surrounding host stromal tissue play a critical role in tumor progression and metastasis, but the molecular nature of this relationship remains largely uncharacterized. Furthermore, although genetic changes of neoplastic cells in tumors contribute significantly to tumor pr...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0609635104

    authors： Pelham RJ,Rodgers L,Hall I,Lucito R,Nguyen KC,Navin N,Hicks J,Mu D,Powers S,Wigler M,Botstein D

    更新日期：2006-12-26 00:00:00

  • Encroaching forests decouple alpine butterfly population dynamics.

    abstract：:Over the past 50 years, the rising tree line along Jumpingpound Ridge in the Rocky Mountains of Alberta, Canada, has reduced the area of alpine meadows and isolated populations that reside within them. By analyzing an 11-year data set of butterfly population sizes for 17 subpopulations along the ridge, we show that fo...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0705511104

    authors： Roland J,Matter SF

    更新日期：2007-08-21 00:00:00

  • A cascade of cytokines mediates mechanical inflammatory hypernociception in mice.

    abstract：:The hypernociceptive effects of cytokines [TNF-alpha, keratinocyte-derived chemokine (KC), and IL-1beta] and their participation in carrageenan (Cg)-induced inflammatory hypernociception in mice were investigated. Nociceptor sensitization (hypernociception) was quantified with an electronic version of the von Frey fil...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0409225102

    authors： Cunha TM,Verri WA Jr,Silva JS,Poole S,Cunha FQ,Ferreira SH

    更新日期：2005-02-01 00:00:00

  • Molecular architecture of Galphao and the structural basis for RGS16-mediated deactivation.

    abstract：:Heterotrimeric G proteins relay extracellular cues from heptahelical transmembrane receptors to downstream effector molecules. Composed of an alpha subunit with intrinsic GTPase activity and a betagamma heterodimer, the trimeric complex dissociates upon receptor-mediated nucleotide exchange on the alpha subunit, enabl...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0801569105

    authors： Slep KC,Kercher MA,Wieland T,Chen CK,Simon MI,Sigler PB

    更新日期：2008-04-29 00:00:00

  • Circadian organization of the mammalian retina.

    abstract：:The mammalian retina contains an endogenous circadian pacemaker that broadly regulates retinal physiology and function, yet the cellular origin and organization of the mammalian retinal circadian clock remains unclear. Circadian clock neurons generate daily rhythms via cell-autonomous autoregulatory clock gene network...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0601940103

    authors： Ruan GX,Zhang DQ,Zhou T,Yamazaki S,McMahon DG

    更新日期：2006-06-20 00:00:00

  • Two yeast nuclear pore complex proteins involved in mRNA export form a cytoplasmically oriented subcomplex.

    abstract：:We sublocalized the yeast nucleoporin Nup82 to the cytoplasmic side of the nuclear pore complex (NPC) by immunoelectron microscopy. Moreover, by in vitro binding assays we showed that Nup82 interacts with the C-terminal region of Nup159, a yeast nucleoporin that previously was also localized to the cytoplasmic side of...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.95.19.11241

    authors： Hurwitz ME,Strambio-de-Castillia C,Blobel G

    更新日期：1998-09-15 00:00:00

  • Iron chaperones PCBP1 and PCBP2 mediate the metallation of the dinuclear iron enzyme deoxyhypusine hydroxylase.

    abstract：:Although cells express hundreds of metalloenzymes, the mechanisms by which apoenzymes receive their metal cofactors are largely unknown. Poly(rC)-binding proteins PCBP1 and PCBP2 are multifunctional adaptor proteins that bind iron and deliver it to ferritin for storage or to prolyl and asparagyl hydroxylases to metall...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1402732111

    authors： Frey AG,Nandal A,Park JH,Smith PM,Yabe T,Ryu MS,Ghosh MC,Lee J,Rouault TA,Park MH,Philpott CC

    更新日期：2014-06-03 00:00:00

  • Human eIF4E promotes mRNA restructuring by stimulating eIF4A helicase activity.

    abstract：:Elevated eukaryotic initiation factor 4E (eIF4E) levels frequently occur in a variety of human cancers. Overexpression of eIF4E promotes cellular transformation by selectively increasing the translation of proliferative and prosurvival mRNAs. These mRNAs possess highly structured 5'-UTRs that impede ribosome recruitme...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1303781110

    authors： Feoktistova K,Tuvshintogs E,Do A,Fraser CS

    更新日期：2013-08-13 00:00:00

  • Mycobacterial infection of macrophages results in membrane-permeable phagosomes.

    abstract：:Cell-mediated immunity is critical for host resistance to tuberculosis. T lymphocytes recognizing antigens presented by the major histocompatibility complex (MHC) class I and class II molecules have been found to be necessary for control of mycobacterial infection. Mice genetically deficient in the generation of MHC c...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.96.26.15190

    authors： Teitelbaum R,Cammer M,Maitland ML,Freitag NE,Condeelis J,Bloom BR

    更新日期：1999-12-21 00:00:00

  • Evidence that a second tumor antigen coded by adenovirus early gene region E1a is required for efficient cell transformation.

    abstract：:The expression of the adenovirus (Ad) early coding region 1a (E1a) is required for virus-induced cell transformation and for the activation of other viral early genes and some cellular genes. Two overlapping early mRNAs of 13S and 12S that are transcribed from this region code for a 289-amino acid protein and a 243-am...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.82.1.163

    authors： Hurwitz DR,Chinnadurai G

    更新日期：1985-01-01 00:00:00

  • Products of a reciprocal chromosome translocation involving the c-myc gene in a murine plasmacytoma.

    abstract：:The structures of the rearranged c-myc gene products derived from a rcp T(12;15) have been investigated in the MPC-11 (IgG2b kappa) plasmacytoma. The rcp T(12;15) in MPC-11 is a reciprocal exchange between the c-myc gene on chromosome 15 and the immunoglobulin gamma 2a switch region (S gamma 2a) on chromosome 12. The ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.81.3.829

    authors： Stanton LW,Yang JQ,Eckhardt LA,Harris LJ,Birshtein BK,Marcu KB

    更新日期：1984-02-01 00:00:00

  • Engineering of blended nanoparticle platform for delivery of mitochondria-acting therapeutics.

    abstract：:Mitochondrial dysfunctions cause numerous human disorders. A platform technology based on biodegradable polymers for carrying bioactive molecules to the mitochondrial matrix could be of enormous potential benefit in treating mitochondrial diseases. Here we report a rationally designed mitochondria-targeted polymeric n...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1210096109

    authors： Marrache S,Dhar S

    更新日期：2012-10-02 00:00:00