Control of ColE1 DNA replication: the rop gene product negatively affects transcription from the replication primer promoter.

abstract：:Synthesis of mouse metallothionein (MT)-I and MT-II is transcriptionally induced by the synthetic glucocorticoid, dexamethasone (DEX) or both in vivo as well as in numerous cell lines. However, the location(s) of a glucocorticoid response element (GRE) has not been described. The observation that a marked MT-I gene, a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kelly EJ,Sandgren EP,Brinster RL,Palmiter RD

更新日期：1997-09-16 00:00:00

abstract：:Recent studies have shown that the genes of the gibberellin (GA) biosynthesis pathway in the fungus Gibberella fujikuroi are organized in a cluster of at least seven genes. P450-1 is one of four cytochrome P450 monooxygenase genes in this cluster. Disruption of the P450-1 gene in the GA-producing wild-type strain IMI ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rojas MC,Hedden P,Gaskin P,Tudzynski B

更新日期：2001-05-08 00:00:00

abstract：:Particles composed of secondary organic material (SOM) are abundant in the lower troposphere. The viscosity of these particles is a fundamental property that is presently poorly quantified yet required for accurate modeling of their formation, growth, evaporation, and environmental impacts. Using two unique techniques...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Renbaum-Wolff L,Grayson JW,Bateman AP,Kuwata M,Sellier M,Murray BJ,Shilling JE,Martin ST,Bertram AK

更新日期：2013-05-14 00:00:00

abstract：:Amoebae of the cellular slime mold Dictyostelium discoideum possess an extensive and dynamic endomembrane system that includes many types of acidic vacuoles. A light membrane fraction from Dictyostelium, rich in vacuolar-type H(+)-ATPase, has been described [Padh, H., Lavasa, M. & Steck, T.L. (1989) J. Cell Biol. 108,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rooney EK,Gross JD

更新日期：1992-09-01 00:00:00

abstract：:The amplitude of the CO(2) seasonal cycle at the Mauna Loa Observatory (MLO) increased from the early 1970s to the early 1990s but decreased thereafter despite continued warming over northern continents. Because of its location relative to the large-scale atmospheric circulation, the MLO receives mainly Eurasian air m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Buermann W,Lintner BR,Koven CD,Angert A,Pinzon JE,Tucker CJ,Fung IY

更新日期：2007-03-13 00:00:00

abstract：:Oligomerization of members of the p53 family of transcription factors (p53, p63, and p73) is essential for their distinct functions in cell-cycle control and development. To elucidate the molecular basis for tetramer formation of the various family members, we solved the crystal structure of the human p73 tetramerizat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Joerger AC,Rajagopalan S,Natan E,Veprintsev DB,Robinson CV,Fersht AR

更新日期：2009-10-20 00:00:00

abstract：:When two phases of water are at equilibrium, the ratio of hydrogen isotopes in each is slightly altered because of their different phase affinities. This isotopic fractionation process can be utilized to analyze water's movement in the world's climate. Here we show that equilibrium fractionation ratios, an entirely qu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Markland TE,Berne BJ

更新日期：2012-05-22 00:00:00

abstract：:Cell-based therapies are attractive for revascularizing and regenerating tissues and organs, but clinical trials of endothelial progenitor cell transplantation have not resulted in consistent benefit. We propose a different approach in which a material delivery system is used to create a depot of vascular progenitor c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Silva EA,Kim ES,Kong HJ,Mooney DJ

更新日期：2008-09-23 00:00:00

abstract：:Many chemoattractants cause chemotaxis of leukocytes by stimulating a structurally distinct class of G protein-coupled receptors. To identify receptor functions required for chemotaxis, we studied chemotaxis in HEK293 cells transfected with receptors for nonchemokine ligands or for interleukin 8 (IL-8), a classical ch...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Neptune ER,Bourne HR

更新日期：1997-12-23 00:00:00

abstract：:Antigen.antibody complexes (Ag.Ab) prepared from (125)I-labeled bovine serum albumin and guinea pig anti-albumin were incubated at 37 degrees C for 30 min with normal human serum diluted optimally for binding (1:16) and then with autologous erythrocytes (RBC). After washing, RBC-bearing antigen.antibody.complement com...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Medof ME,Prince GM,Mold C

更新日期：1982-08-01 00:00:00

abstract：:Rapid and efficient protocols to generate oligodendrocytes (OL) from human induced pluripotent stem cells (iPSC) are currently lacking, but may be a key technology to understand the biology of myelin diseases and to develop treatments for such disorders. Here, we demonstrate that the induction of three transcription f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ehrlich M,Mozafari S,Glatza M,Starost L,Velychko S,Hallmann AL,Cui QL,Schambach A,Kim KP,Bachelin C,Marteyn A,Hargus G,Johnson RM,Antel J,Sterneckert J,Zaehres H,Schöler HR,Baron-Van Evercooren A,Kuhlmann T

更新日期：2017-03-14 00:00:00

abstract：:Photosynthetic water splitting, coupled to hydrogenase-catalyzed hydrogen production, is considered a promising clean, renewable source of energy. It is widely accepted that the oxygen sensitivity of hydrogen production, combined with competition between hydrogenases and NADPH-dependent carbon dioxide fixation are the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yacoby I,Pochekailov S,Toporik H,Ghirardi ML,King PW,Zhang S

更新日期：2011-06-07 00:00:00

abstract：:Early forms of the genetic code likely generated "statistical" proteins, with similar side chains occupying the same sequence positions at different ratios. In this scenario, groups of related side chains were treated by aminoacyl-tRNA synthetases as a single molecular species until a discrimination mechanism develope...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yang XL,Otero FJ,Skene RJ,McRee DE,Schimmel P,Ribas de Pouplana L

更新日期：2003-12-23 00:00:00

abstract：:Evolution of an oxygenic atmosphere required primordial life to accommodate the toxicity associated with reactive oxygen species. We have characterized an archaeal antioxidant from the hyperthermophilic acidophile Sulfolobus solfataricus. The amino acid sequence of this approximately 22-kDa protein shares little seque...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wiedenheft B,Mosolf J,Willits D,Yeager M,Dryden KA,Young M,Douglas T

更新日期：2005-07-26 00:00:00

abstract：:A method has been developed for de novo peptide sequencing using matrix-assisted laser desorption ionization mass spectrometry. This method will facilitate biological studies that require rapid determination of peptide or protein sequences, e.g., determination of posttranslational modifications, identification of acti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Keough T,Youngquist RS,Lacey MP

更新日期：1999-06-22 00:00:00

abstract：:We have earlier proposed a molecular mechanism for the translocation of hydrophilic proteins across membranes that accounts for the experimental facts and meets the restrictions that we stipulate for such a mechanism. In particular, the restrictions are that translocation occurs by successive segments of the polypepti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Singer SJ,Maher PA,Yaffe MP

更新日期：1987-04-01 00:00:00

abstract：:Steroidogenic acute regulatory protein (StAR) appears to mediate the rapid increase in pregnenolone synthesis stimulated by tropic hormones. cDNAs encoding StAR were isolated from a human adrenal cortex library. Human StAR, coexpressed in COS-1 cells with cytochrome P450scc and adrenodoxin, increased pregnenolone synt...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sugawara T,Holt JA,Driscoll D,Strauss JF 3rd,Lin D,Miller WL,Patterson D,Clancy KP,Hart IM,Clark BJ

更新日期：1995-05-23 00:00:00

abstract：:The ever-increasing global need for potable water requires practical, sustainable approaches for purifying abundant alternative sources such as seawater, high-salinity processed water, or underground reservoirs. Evaporation-based solutions are of particular interest for treating high salinity water, since conventional...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dongare PD,Alabastri A,Neumann O,Nordlander P,Halas NJ

更新日期：2019-07-02 00:00:00

abstract：:I demonstrate the existence in protein domain sequences of sets of statistically significant periodic signals, characteristic of the architectures of those domains. It is shown that, although the frequencies of the signals characteristic of a particular architecture have definite wave numbers, they can occur in any ph...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rackovsky S

更新日期：1998-07-21 00:00:00

abstract：:High titered IgE, IgG, and IgM antibody responses to the major antigenic determinant of penicillin, the benzylpenicilloyl hapten, were elicited by the intraperitoneal injection of the hapten coupled to keyhole limpet hemocyanin mixed with the appropriate adjuvant. However, treatment of such mice with the benzylpenicil...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chiorazzi N,Eshhar Z,Katz DH

更新日期：1976-06-01 00:00:00

abstract：:Selective culling in populations of most organisms is rank-order: individuals of low rank on a scale of potential fitnesses tend to be eliminated during early development, whereas surviving adults (whose number reflects the carrying capacity of the environment) are generally drawn from the distribution's upper end. Ha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wallace B

更新日期：1989-03-01 00:00:00

abstract：:We performed the biochemical and biophysical characterization of a red fluorescent protein, eqFP611, from the sea anemone Entacmaea quadricolor cloned in Escherichia coli. With an excitation maximum at 559 nm and an emission maximum at 611 nm, the recombinant protein shows the most red-shifted emission and the largest...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wiedenmann J,Schenk A,Röcker C,Girod A,Spindler KD,Nienhaus GU

更新日期：2002-09-03 00:00:00

abstract：:Maternal microchimerism (MMc) has been associated with development of allospecific transplant tolerance, antitumor immunity, and cross-generational reproductive fitness, but its mode of action is unknown. We found in a murine model that MMc caused exposure to the noninherited maternal antigens in all offspring, but in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bracamonte-Baran W,Florentin J,Zhou Y,Jankowska-Gan E,Haynes WJ,Zhong W,Brennan TV,Dutta P,Claas FH,van Rood JJ,Burlingham WJ

更新日期：2017-01-31 00:00:00

abstract：:Radiation therapy is a widely used cancer treatment, but it causes side effects even when localized radiotherapy is used. Extensive apoptosis of microvascular endothelial cells of the lamina propria is the primary lesion initiating intestinal radiation damage after abdominal radiation therapy. Many in vitro studies su...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cho CH,Kammerer RA,Lee HJ,Yasunaga K,Kim KT,Choi HH,Kim W,Kim SH,Park SK,Lee GM,Koh GY

更新日期：2004-04-13 00:00:00

abstract：:We have obtained the resonance Raman spectrum of bacteriorhodopsin's primary photoproduct K with a novel low-temperature spinning sample technique. Purple membrane at 77 K is illuminated with spatially separated actinic (pump) and probe laser beams. The 514-nm pump beam produces a photostationary steady-state mixture ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Braiman M,Mathies R

更新日期：1982-01-01 00:00:00

abstract：:The RuvC protein of Escherichia coli catalyzes the resolution of recombination intermediates during genetic recombination and the recombinational repair of damaged DNA. Resolution involves specific recognition of the Holliday structure to form a complex that exhibits twofold symmetry with the DNA in an open configurat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bennett RJ,West SC

更新日期：1996-10-29 00:00:00

abstract：:Copper homeostasis is essential for bacterial pathogen fitness and infection, and has been the focus of a number of recent studies. In Salmonella, envelope protection against copper overload and macrophage survival depends on CueP, a major copper-binding protein in the periplasm. This protein is also required to deliv...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pezza A,Pontel LB,López C,Soncini FC

更新日期：2016-10-11 00:00:00

abstract：:A transient transfection assay using Drosophila S2 tissue culture cells and WT and mutant Drosophila vnd/NK-2 homeobox cDNAs was used to localize repression and activation domains of vnd/NK-2 homeodomain protein. A repression domain was identified near the N terminus of vnd/NK-2 homeodomain protein (amino acid residue...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stepchenko A,Nirenberg M

更新日期：2004-09-07 00:00:00

abstract：:The int-1 and int-2 genes were first isolated as targets for transcriptional activation by proviral insertion mutations in mammary carcinomas induced by the mouse mammary tumor virus (MMTV). Since these proto-oncogenes are not expressed at detectable levels in previously tested normal tissues from adult mice, we sough...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jakobovits A,Shackleford GM,Varmus HE,Martin GR

更新日期：1986-10-01 00:00:00

abstract：:Split GFPs have been widely applied for monitoring protein-protein interactions by expressing GFPs as two or more constituent parts linked to separate proteins that only fluoresce on complementing with one another. Although this complementation is typically irreversible, it has been shown previously that light acceler...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lin CY,Both J,Do K,Boxer SG

更新日期：2017-03-14 00:00:00