当前位置： SCI文献检索 > INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE期刊下所有文献 > IL-33 shifts macrophage polarization, promoting resistance against Pseudomonas aeruginosa keratitis.

IL-33 shifts macrophage polarization, promoting resistance against Pseudomonas aeruginosa keratitis.

Abstract：

PURPOSE:To determine the role of IL-33 in resistance to Pseudomonas aeruginosa keratitis. METHODS:Corneal IL-33 mRNA and protein levels were tested in susceptible C57BL/6 (B6) and resistant BALB/c mice. B6 mice were injected with recombinant mouse IL-33 (rmIL-33) and disease severity, bacterial load, polymorphonuclear neutrophils (PMN) infiltrate, gene expression of inflammatory, and T-helper (Th)1/Th2 cytokines were tested by RT-PCR. IL-33 signaling and macrophage (Mvarphi) polarization also were examined. RESULTS:IL-33 mRNA and protein were expressed constitutively in the normal corneas of both groups and were significantly elevated at 1 to 5 days after infection in BALB/c over B6 mice. rmIL-33-treated B6 mice showed less severe disease than did PBS controls and exhibited decreased bacterial load, PMN infiltrate, and corneal mRNA levels for IL-1beta, MIP-2, and TNF-alpha. Th2-type cytokines (IL-4, -5, -10) also were significantly upregulated, and protein levels for TNF-alpha and IL-10 confirmed the mRNA data. To further investigate IL-33 in corneal inflammation, it was overexpressed in Mvarphi (RAW264.7 cells). This significantly increased IL-5 and IL-10, while it decreased IFN-gamma and other pro-inflammatory cytokines. The role of the Mvarphi was further tested in infected rmIL-33 compared with PBS-injected mice. Immunostaining showed that rmIL-33 injection shifted Mvarphi polarization from NO synthase 2 to arginase production. Furthermore, peritoneally elicited cells (B6 mice) treated with lipopolysaccharide and rmIL-33 exhibited elevated ST2 levels and a shift from IL-12 to IL-10 mRNA production. CONCLUSIONS:These data provide evidence that IL-33 promotes a Th2-type immune response and reduces inflammation by polarizing the Mvarphi production of anti-inflammatory mediators in the cornea.

journal_name

Invest Ophthalmol Vis Sci

journal_title

Investigative ophthalmology & visual science

authors

Hazlett LD,McClellan SA,Barrett RP,Huang X,Zhang Y,Wu M,van Rooijen N,Szliter E

doi

10.1167/iovs.09-3983

subject

Has Abstract

pub_date

2010-03-01 00:00:00

pages

1524-32

issue

3

eissn

0146-0404

issn

1552-5783

pii

iovs.09-3983

journal_volume

51

pub_type

杂志文章

相关文献

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE文献大全
  • Motion perception in glaucoma.

    abstract：PURPOSE:This study was performed to investigate motion perception in patients with glaucoma. METHODS:A random dot motion test was used to measure three aspects of central motion perception: minimum displacement threshold (Dmin), maximum displacement threshold (Dmax), and coherence threshold (signal to noise). Motion p...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Bullimore MA,Wood JM,Swenson K

    更新日期：1993-12-01 00:00:00

  • Comparative transcriptome and network biology analyses demonstrate antiproliferative and hyperapoptotic phenotypes in human keratoconus corneas.

    abstract：PURPOSE:To decipher the biological pathways involved in keratoconus pathophysiology by determining the patterns of differential gene expression between keratoconus and control corneas. METHODS:RNA was extracted from surgically removed corneas of 10 keratoconus patients and from normal corneas of 10 control patients wh...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.10-70981

    authors： Macé M,Galiacy SD,Erraud A,Mejía JE,Etchevers H,Allouche M,Desjardins L,Calvas P,Malecaze F

    更新日期：2011-08-03 00:00:00

  • Mechanism of adrenomedullin-induced relaxation in isolated canine retinal arteries.

    abstract：PURPOSE:To analyze the mechanism of action of adrenomedullin (AM), a peptide recently isolated from human pheochromocytoma, in isolated canine central retinal arteries and to compare the action of calcitonin gene-related peptide (CCRP). METHODS:Changes in isometric tension were recorded in helical strips of the arteri...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Okamura T,Ayajiki K,Kangawa K,Toda N

    更新日期：1997-01-01 00:00:00

  • Difluprednate 0.05% versus prednisolone acetate 1% for endogenous anterior uveitis: a phase III, multicenter, randomized study.

    abstract：PURPOSE:Endogenous anterior uveitis (AU), when untreated, may lead to vision loss. This study compared the safety and efficacy of difluprednate versus prednisolone acetate for the treatment of this condition. METHODS:This phase III, double-masked, noninferiority study randomized patients with mild to moderate endogeno...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章,多中心研究,随机对照试验

    doi：10.1167/iovs.13-12660

    authors： Sheppard JD,Toyos MM,Kempen JH,Kaur P,Foster CS

    更新日期：2014-05-06 00:00:00

  • Topical Treatment With Brimonidine and Somatostatin Causes Retinal Vascular Dilation in Patients With Early Diabetic Retinopathy From the EUROCONDOR.

    abstract：Purpose:Structural retinal microvascular changes have been identified as risk markers of diabetic retinopathy (DR). In order to estimate the retinal response of neuroprotective eye drops, we aimed to evaluate the effect of topical retinal neuroprotection on retinal microvascular changes in early DR. Methods:Patients w...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章,多中心研究

    doi：10.1167/iovs.18-26487

    authors： Grauslund J,Frydkjaer-Olsen U,Peto T,Fernández-Carneado J,Ponsati B,Hernández C,Cunha-Vaz J,Simó R,EUROCONDOR.

    更新日期：2019-05-01 00:00:00

  • The role of macrophages in the pathogenesis of HSV-1 induced chorioretinitis in BALB/c mice.

    abstract：PURPOSE:To examine the effects of modification of immune effector cells, including macrophages, in the pathogenesis of herpes simplex virus retinitis in BALB/c mice. METHODS:Two intravitreal injections (2 microliters each) of anti-CD11b monoclonal antibody (mAb) [13 micrograms/microliters] were administered to the con...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Berra A,Rodriguez A,Heiligenhaus A,Pazos B,Van Rooijen N,Foster CS

    更新日期：1994-06-01 00:00:00

  • Heterozygous Loss of Yap1 in Mice Causes Progressive Cataracts.

    abstract：Purpose:Yap1 encodes an evolutionarily conserved transcriptional coactivator and functions as a down-stream effector of the Hippo signaling pathway that controls tissue size and cell growth. Yap1 contributes to lens epithelial development. However, the effect of Yap1 haplodeficiency on the lens epithelium and its role ...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.61.12.21

    authors： Lu Q,Zhang Y,Kasetti RB,Gaddipati S,Cvm NK,Borchman D,Li Q

    更新日期：2020-10-01 00:00:00

  • Detection and subcellular localization of two 15S-lipoxygenases in human cornea.

    abstract：PURPOSE:There are two human 15-lipoxygenases (LOX), 15-LOX-1 and -2, which convert arachidonic acid to 15S-hydroxyeicosatetraenoic acid (15S-HETE). The presence of both 15-LOXs in the human cornea prompted this study to delineate their roles in the human corneal epithelium. METHODS:Human corneal epithelia from donor c...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.04-1166

    authors： Chang MS,Schneider C,Roberts RL,Shappell SB,Haselton FR,Boeglin WE,Brash AR

    更新日期：2005-03-01 00:00:00

  • Upper punctal occlusion versus lower punctal occlusion in dry eye.

    abstract：PURPOSE:To compare the effectiveness of upper punctal occlusion versus that of lower punctal occlusion in dry eye patients. METHODS:One eye's upper punctum and the contralateral eye's lower punctum were occluded with collagen plugs in 20 dry eye patients. The same procedure was performed in 20 normal subjects. The upp...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章,随机对照试验

    doi：10.1167/iovs.09-5097

    authors： Chen F,Wang J,Chen W,Shen M,Xu S,Lu F

    更新日期：2010-11-01 00:00:00

  • Angiotensin II-stimulated vascular endothelial growth factor expression in bovine retinal pericytes.

    abstract：PURPOSE:Angiotensin II (AII) has been shown to play a role in many vascular diseases. In the study described, the effect of AII on vascular endothelial growth factor (VEGF) expression and related intracellular signaling mechanism was investigated in bovine retinal microcapillary pericytes. METHODS:Cultured bovine reti...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Otani A,Takagi H,Oh H,Suzuma K,Matsumura M,Ikeda E,Honda Y

    更新日期：2000-04-01 00:00:00

  • The association between time spent outdoors and myopia using a novel biomarker of outdoor light exposure.

    abstract：PURPOSE:We sought to determine whether conjunctival ultraviolet autofluorescence (UVAF), a biomarker of outdoor light exposure, is associated with myopia. METHODS:We performed a cross-sectional study on Norfolk Island and recruited individuals aged ≥ 15 years. Participants completed a sun-exposure questionnaire and un...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.11-8677

    authors： Sherwin JC,Hewitt AW,Coroneo MT,Kearns LS,Griffiths LR,Mackey DA

    更新日期：2012-07-01 00:00:00

  • The effect of TGF-beta1 on differential gene expression profiles in human corneal epithelium studied by cDNA expression array.

    abstract：PURPOSE:TGF-betas regulate cell proliferation and differentiation, and they play important roles in maintenance of corneal epithelium. However, the precise function of TGF-betas in the corneal epithelium remains unclear. In this study, cDNA expression array technology was used to demonstrate the effect of TGF-beta1 on ...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Hayashida-Hibino S,Watanabe H,Nishida K,Tsujikawa M,Tanaka T,Hori Y,Saishin Y,Tano Y

    更新日期：2001-07-01 00:00:00

  • RPE cell surface proteins in normal and dystrophic rats.

    abstract：:Membrane-bound proteins in plasma membrane enriched fractions from cultured rat RPE were analyzed by two-dimensional gel electrophoresis. Membrane proteins were characterized on three increasingly specific levels. Total protein was visualized by silver staining. A maximum of 102 separate proteins were counted in silve...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Clark VM,Hall MO

    更新日期：1986-02-01 00:00:00

  • Humidity effects on corneal hydration.

    abstract：:Overall corneal hydration control expressed as the percent recovery per hour (PRPH) can be assessed with an exponential model that uses data derived from two kinds of corneal thickness measurements; one from monitoring recovery after inducing corneal swelling, and the other from measurements made after the eye has bee...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Cohen SR,Polse KA,Brand RJ,Mandell RB

    更新日期：1990-07-01 00:00:00

  • Comparison of Visual Field Progression Rates Among the High Tension Glaucoma, Primary Angle Closure Glaucoma, and Normal Tension Glaucoma.

    abstract：Purpose:To compare the visual field (VF) progression among high tension glaucoma (HTG), primary angle closure glaucoma (PACG), and normal tension glaucoma (NTG) subjects in routine clinical care. Methods:All patients had ≥5 VF tests using HFA, 24-2, SITA-standard strategy. We compared the progression between glaucoma ...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.18-25421

    authors： Ballae Ganeshrao S,Senthil S,Choudhari N,Sri Durgam S,Garudadri CS

    更新日期：2019-03-01 00:00:00

  • Retinal Nerve Fiber Layer Thickness in Children: The Gobi Desert Children Eye Study.

    abstract：Purpose:Because there is a paucity of population-based data on retinal nerve fiber layer thickness (RNFLT) for children, we measured the RNFLT and its associations in schoolchildren. Methods:The population-based Gobi Desert Children Eye Study included all schoolchildren aged 6 to 21 years living in Ejina, Inner Mongol...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.18-25418

    authors： Wang CY,Zheng YF,Liu B,Meng ZW,Hong F,Wang XX,Wang XJ,Du L,Wang IY,Zhu D,Tao Y,You QS,Jonas JB

    更新日期：2018-10-01 00:00:00

  • Psychophysical investigations of the temporal modulation sensitivity function in amblyopia: uniform field flicker.

    abstract：:The temporal modulation sensitivity function was studied in eight strabismic and/or anisometropic amblyopes and in two subjects with amblyopia resulting from monocular stimulus deprivation (cataract and ptosis). Half of the strabismic/anisometropic amblyopes showed a reduction in the sensitivity of the amblyopic eye. ...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Manny RE,Levi DM

    更新日期：1982-04-01 00:00:00

  • Generation and Proteome Profiling of PBMC-Originated, iPSC-Derived Corneal Endothelial Cells.

    abstract：Purpose:Corneal endothelial cells (CECs) are critical in maintaining clarity of the cornea. This study was initiated to develop peripheral blood mononuclear cell (PBMC)-originated, induced pluripotent stem cell (iPSC)-derived CECs. Methods:We isolated PBMCs and programmed the mononuclear cells to generate iPSCs, which...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.17-22927

    authors： Ali M,Khan SY,Vasanth S,Ahmed MR,Chen R,Na CH,Thomson JJ,Qiu C,Gottsch JD,Riazuddin SA

    更新日期：2018-05-01 00:00:00

  • Head shaking and vestibulo-ocular reflex in congenital nystagmus.

    abstract：:The authors investigated the mechanisms underlying the head shaking shown by some patients with congenital nystagmus (CN). In order to improve visual function by head shaking, a patient with CN must have some visual acuity loss due to retinal image motion created by the nystagmus; an abnormal vestibulo-ocular reflex (...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Carl JR,Optican LM,Chu FC,Zee DS

    更新日期：1985-08-01 00:00:00

  • The oscillatory potentials of the mudpuppy retina.

    abstract：:The properties of the oscillatory potentials (OPs) of the mudpuppy electroretinogram (ERG) were studied and compared with the properties of the b-wave of the ERG and the proximal negative response (PNR). Both transretinal and intraretinal ERGs were recorded in response to full-field as well as 200 and 500 microgram sp...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Wachtmeister L,Dowling JE

    更新日期：1978-12-01 00:00:00

  • Screening of ocular enterobacteriaceae isolates for presence of chromosomal blaNDM-1 and ESBL genes: a 2-year study at a tertiary eye care center.

    abstract：PURPOSE:Since, to our knowledge, there are no reports on the prevalence of the blaNDM-1 gene among ocular isolates of Enterobacteriaceae, and only limited information on the prevalence of extended spectrum beta-lactamases (ESBLs) among ocular bacterial isolates are available, our study was undertaken. METHODS:A prospe...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.12-10467

    authors： Sowmiya M,Malathi J,Madhavan HN

    更新日期：2012-08-07 00:00:00

  • The effect of blood on ocular fundus reflectance and determination of some optical properties of retinal blood vessels.

    abstract：:Light reflected from 50 micrometer diameter spots on the fundi of two adult rhesus monkeys was measured as a function of wavelength in 10 nm jumps from 400 to 900 nm. The areas measured were a retinal artery and vein, areas of the disk, macula, and retina devoid of visible blood vessels, and the foveola. The eyes of t...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Flower RW,McLeod DS,Pitts SM

    更新日期：1978-06-01 00:00:00

  • Recording the contralateral PERG: effect of different electrodes.

    abstract：:A pattern electroretinogram (PERG) can be recorded when the eye wearing the electrode is occluded and the stimulus is viewed with the other eye. We find that this phenomenon occurs when an Arden gold foil electrode is used, but not when either an ERG-Jet lens or a scleral lens electrode is used. Therefore, a corneal-t...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Peachey NS,Sokol S,Moskowitz A

    更新日期：1983-11-01 00:00:00

  • Clinical significance of saccade analysis in early active Graves' ophthalmopathy.

    abstract：PURPOSE:To assess whether saccadic eye movements show distinct changes in patients with early active Graves' ophthalmopathy (GO), which could serve as a diagnostic tool for early detection and treatment. METHODS:Each of two prospective studies included 10 patients with early acute GO and 10 age- and sex-matched contro...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Schworm HD,Heufelder AE,Kunze A,Welge E,Boergen KP

    更新日期：2000-06-01 00:00:00

  • Relationship between retinal layer thickness and the visual field in early age-related macular degeneration.

    abstract：PURPOSE:To quantify and compare the structural and functional changes in subjects with early age-related macular degeneration (AMD), using spectral-domain optical coherence tomography (SD-OCT) and microperimetry. METHODS:Twenty-one eyes of 21 subjects with early AMD were examined. MP-1 10-2 visual fields (VFs) and SD-...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.12-10361

    authors： Acton JH,Smith RT,Hood DC,Greenstein VC

    更新日期：2012-11-09 00:00:00

  • Increase of manganese superoxide dismutase, but not of Cu/Zn-SOD, in experimental optic neuritis.

    abstract：PURPOSE:To evaluate the role of manganese superoxide dismutase (Mn-SOD) and copper/zinc superoxide dismutase (Cu/Zn-SOD) in cellular protection of the optic nerve against the oxidative injury that contributes to demyelination in experimental allergic encephalomyelitis (EAE). METHODS:Immunocytochemistry for Mn-SOD and ...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Qi X,Guy J,Nick H,Valentine J,Rao N

    更新日期：1997-05-01 00:00:00

  • A new noninvasive tear stability analysis system for the assessment of dry eyes.

    abstract：PURPOSE:This was a prospective case-control study conducted to evaluate the effectiveness of the Tear Stability Analysis System (TSAS) for the assessment of patients with dry eye. METHODS:The TSAS can take 10 consecutive corneal topograms at one per second for 10 seconds. Examinations using the TSAS were conducted in ...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.03-0712

    authors： Kojima T,Ishida R,Dogru M,Goto E,Takano Y,Matsumoto Y,Kaido M,Ohashi Y,Tsubota K

    更新日期：2004-05-01 00:00:00

  • The role of Src family kinases in cortical cataract formation.

    abstract：PURPOSE:The goal of this study was to determine the role of Src family kinases (SFKs) in the development of lens cataract. This question was particularly significant, because these tyrosine kinases mediate the stress pathways known to lead to cataract formation. The experiments were focused on whether the inhibition of...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Zhou J,Menko AS

    更新日期：2002-07-01 00:00:00

  • Differential expression of chemokines by human retinal pigment epithelial cells infected with cytomegalovirus.

    abstract：PURPOSE:To evaluate the effects of human cytomegalovirus (HCMV) infection on chemokine gene expression and secretion by human retinal pigment epithelial (HRPE) cell cultures. METHODS:HRPE cells were infected with HCMV (strain AD169) at an MOI of 5. Culture supernatants, collected at various postinoculation days, were ...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.02-0980

    authors： Momma Y,Nagineni CN,Chin MS,Srinivasan K,Detrick B,Hooks JJ

    更新日期：2003-05-01 00:00:00

  • BBSome Component BBS5 Is Required for Cone Photoreceptor Protein Trafficking and Outer Segment Maintenance.

    abstract：Purpose:To identify the role of the BBSome protein Bardet-Biedl syndrome 5 (BBS5) in photoreceptor function, protein trafficking, and structure using a congenital mutant mouse model. Methods:Bbs5-/- mice (2 and 9 months old) were used to assess retinal function and morphology. Hematoxylin and eosin staining of retinal...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.61.10.17

    authors： Bales KL,Bentley MR,Croyle MJ,Kesterson RA,Yoder BK,Gross AK

    更新日期：2020-08-03 00:00:00