Abstract：
:Protein translocation is a universal event shared by most cell signalling pathways to transmit signals between cell compartments. In recent years, the use of new fluorescence microscopy technologies combined with fluorescent probes--most often fluorescent proteins--and image analysis software has allowed the visualization and extensive analysis of such dynamic events in the context of the living cell. This review article focuses on emerging fluorescence approaches that tackle live cell protein translocation in the image-free context. Such methods are based on either protein-protein interactions or analysis of spatial diffusion of proteins by fluorescence intensity measurements. The potential benefits of intensity measurement on global cell populations versus image analysis of heterogeneous cell sample are discussed in the context of drug discovery applications.
journal_name
Curr Opin Pharmacoljournal_title
Current opinion in pharmacologyauthors
Furger C,Derick S,Boutin JA,Nosjean Odoi
10.1016/j.coph.2009.07.002subject
Has Abstractpub_date
2009-10-01 00:00:00pages
650-6issue
5eissn
1471-4892issn
1471-4973pii
S1471-4892(09)00101-5journal_volume
9pub_type
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