Abstract:
:In this communication, the roles of apoptosis-inducing factor (AIF) and Bcl-2 inhibitor of transcription (Bit1) in TAp63gamma-induced apoptosis were investigated in human esophageal squamous cancer EC9706 cells. Positive RNA and protein expressions of AIF and Bit1 in these cells were verified. However, no TAp63gamma could be detected. Transfection of expression vector pcDNA3.1-TAp63gamma into EC9706 resulted in TAp63gamma expression, and peak level apoptosis was observed 24 h after the transfection disclosed by DNA fragmentation assay. In addition, it was found with Western blot that AIF and Bit1 were released into cytosol from mitochondria, and AIF was further translocated into nucleus, during the stage of TAp63gamma-induced cell apoptosis. Down-regulation of either AIF or Bit1 by RNA interference could, however, alleviate TAp63gamma-induced cell apoptosis. In conclusion, TAp63gamma could induce apoptosis in human esophageal squamous cancer EC9706 cells, through at least releasing AIF and Bit1 from mitochindria into cytosol and nucleus, where apoptotic cascade takes place. These findings indicate that mitochondria-released proapoptotic proteins, AIF and Bit1, are important factors in a TAp63gamma-induced EC9706 cell apoptosis pathway.
journal_name
Int J Oncoljournal_title
International journal of oncologyauthors
Fan T,Jiang G,Suo Z,Liu H,Xu P,Ji Z,Zhang L,Yang Gsubject
Has Abstractpub_date
2009-08-01 00:00:00pages
359-67issue
2eissn
1019-6439issn
1791-2423journal_volume
35pub_type
杂志文章abstract::The efficacy of radioimmunotherapy with radiolabelled monoclonal antibody (Mab) depends on the amount of antibody taken up by the tumour and on its intratumoral distribution. Multicellular spheroids (MTS) of lung cell carcinoma was investigated to study cellular and subcellular distribution of a Mab Po66 labelled with...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.10.3.609
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abstract::The human ovarian cancer cell line OV-MZ-19, established from a patient with cystadenocarcinoma of the ovary, expressing thrombomodulin (TM), a cell surface receptor for the serine protease thrombin, interacts with monoclonal and polyclonal antibodies having different specificity for TM. These antibodies detect TM ant...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.13.4.645
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abstract::Hepatocyte growth factor (HGF) is a key growth factor linked to promoting cancer progression and angiogenesis. The present study identifies repulsive guidance molecule b (RGMb), a bone morphogenetic protein (BMP) co-receptor, as a gene whose expression is regulated by HGF and explores the potential of RGMb to contribu...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2508
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journal_title:International journal of oncology
pub_type: 杂志文章
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更新日期:2016-08-01 00:00:00
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abstract::In order to realise the full potential of gene therapy as a rational approach to the treatment of cancer, it will be necessary to achieve delivery of the therapeutic gene selectively to target tumour cells. Such cancer cell-specific gene delivery is mandated in the context of locoregional or compartmentalised carcinom...
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journal_title:International journal of oncology
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journal_title:International journal of oncology
pub_type: 杂志文章
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journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2009-02-01 00:00:00
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pub_type: 杂志文章,评审
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journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2001-03-01 00:00:00
abstract::To elucidate the involvement of proteasome inhibition in apoptosis induced by anthracycline anticancer agents, we investigated the interaction between the proteasome and anthracycline anticancer agents, and the function of the proteasome in apoptosis. Exposure of L1210 mouse lymphocytic leukemia cells to adriamycin (A...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2002-06-01 00:00:00
abstract::Growth factors may play a role in autocrine or paracrine growth control of tumour cells. We have now examined the expression pattern in vivo by in situ hybridization (ISH) on a series of 13 ductal adenocarcinomas of the pancreas using the non-radioactive digoxigenin system to generate specific antisense orientated rib...
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journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2004-03-01 00:00:00
abstract::One of the early events of apoptosis is proteolysis. The enzyme(s) involved in this event appear to be the serine protease(s), inasmuch as the reversible or irreversible inhibitors of serine proteases prevent DNA degradation and abrogate other signs of apoptosis in several cell systems (Gorczyca et al, Int J Oncol 1: ...
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pub_type: 杂志文章
doi:10.3892/ijo.3.4.627
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journal_title:International journal of oncology
pub_type: 临床试验,杂志文章
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journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2006-04-01 00:00:00
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pub_type: 杂志文章
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journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2002-09-01 00:00:00
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pub_type: 已发布勘误
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pub_type: 杂志文章
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更新日期:2013-09-01 00:00:00