Abstract：
:Studies suggest Candida albicans infection has a negative effect on sperm function, including fertilizing ability. Assisted reproduction treatment using spermatozoa from a patient with unrecognized C. albicans infection did not result in fertilization. Preliminary evidence suggested an effect on sperm motility and apoptosis. This study was undertaken to evaluate the effects of experimentally induced C. albicans infection on motility, membrane mitochondrial potential (MMP), chromatin packaging and apoptosis [membrane phosphatidylserine (PS) externalization and DNA fragmentation] of spermatozoa isolated from normozoospermic healthy men. Motile spermatozoa were isolated by swim-up from 13 normal volunteers and exposed to increasing concentrations (0, 1000, 10,000, and 100,000 cfu/ml) of the fungus for 3 and 24 h. C. albicans was isolated from vaginal swabs, after identification, freshly prepared for experiments. Following incubation, sperm motility decreased significantly (P < 0.05 from 10,000 cfu/ml) and spermatozoa with reduced MMP or PS externalization, an early sign of apoptosis, increased in a time- and concentration-dependent manner. Sperm DNA fragmentation and chromatin integrity increased slightly after exposure to C. albicans, but the increase did not reach statistical significance. This study showed that C. albicans infection may decrease the functional competence of spermatozoa by reducing motility and MMP and by promoting molecular apoptosis mechanisms.
journal_name
Reprod Biomed Onlinejournal_title
Reproductive biomedicine onlineauthors
Burrello N,Salmeri M,Perdichizzi A,Bellanca S,Pettinato G,D'Agata R,Vicari E,Calogero AEdoi
10.1016/s1472-6483(10)60125-3subject
Has Abstractpub_date
2009-04-01 00:00:00pages
496-501issue
4eissn
1472-6483issn
1472-6491pii
S1472-6483(10)60125-3journal_volume
18pub_type
杂志文章abstract：:The 20th century witnessed the steady development of knowledge about reproductive physiology and endocrinology in animals and humans. These advances led to the identification of higher centres governing the ovary and its follicles. Effects of X-rays on the head and other agents, or excision of the pituitary gland, led...
journal_title：Reproductive biomedicine online
pub_type： 杂志文章,评审
doi：10.1016/s1472-6483(12)60006-6
更新日期：2002-01-01 00:00:00
abstract：:Discussion about the ethics of human embryonic stem cell (ESC) research in the UK tends to be dominated by the divisive and potentially intractable issue of the moral status of the embryo. This can have the effect of silencing or marginalizing other concerns, especially in the context of public engagement with science...
journal_title：Reproductive biomedicine online
pub_type： 杂志文章,评审
doi：10.1016/j.rbmo.2010.10.001
更新日期：2010-12-01 00:00:00
abstract：:A prospective randomized trial was performed to compare post-thaw development of murine blastocysts following programmable rate freezing and two methods of vitrification. Frozen 2-cell murine embryos (n = 429) thawed and cultured for 48 h, were randomly allocated by stage of development into four groups: control (not ...
journal_title：Reproductive biomedicine online
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s1472-6483(10)61103-0
更新日期：2004-05-01 00:00:00
abstract：RESEARCH QUESTION:Which factors are associated with reproductive outcomes among infertile women undergoing myomectomy for intramural fibroids? DESIGN:This was a historical cohort study including 127 infertile women who underwent myomectomy due to intramural fibroids as part of fertility enhancement treatment at a sing...
journal_title：Reproductive biomedicine online
pub_type： 杂志文章
doi：10.1016/j.rbmo.2019.04.130
更新日期：2019-09-01 00:00:00
abstract：:The revolutionary development of biotechnology-derived therapeutic proteins has provided the expected improvements in quality, purity and consistency, as demonstrated in recombinant human FSH (rhFSH). However, the development of urine-derived gonadotrophins has not always shown comparable improvements. More recently, ...
journal_title：Reproductive biomedicine online
pub_type： 杂志文章
doi：10.1016/s1472-6483(10)60613-x
更新日期：2006-08-01 00:00:00
abstract：:Egg sharing remains a controversial practice, mainly because of the presumed element of payment. In order to find out to what extent financial considerations motivated the women to share their oocytes, the data on egg sharing in Belgium are analysed. Belgium began providing full reimbursement for six in-vitro fertiliz...
journal_title：Reproductive biomedicine online
pub_type： 杂志文章
doi：10.1016/s1472-6483(10)62009-3
更新日期：2006-07-01 00:00:00
abstract：:In December 2017, Alain Pottage published an article denying the patentability of a technique called Time-Lapse Monitoring (TLM). His argument was that this procedure, like all other embryo selection procedures, fails to respect human dignity. Here, we argue that this statement is mistaken because assisted reproductiv...
journal_title：Reproductive biomedicine online
pub_type： 评论,杂志文章
doi：10.1016/j.rbmo.2018.08.010
更新日期：2018-10-01 00:00:00
abstract：:Pre-eclampsia is a major disorder of human pregnancy, which may have an immunological basis. It is a disease of two stages. The first stage concerns the relative failure of early trophoblast invasion and remodelling of the spiral arteries, leading to a poor blood supply to the placenta, exposing it to oxidative stress...
journal_title：Reproductive biomedicine online
pub_type： 杂志文章,评审
doi：10.1016/s1472-6483(10)60659-1
更新日期：2006-11-01 00:00:00
abstract：:The effect of increasing donor body mass index (BMI) on clinical pregnancies was retrospectively analysed in a cohort of consecutive 2722 donor oocyte IVF cycles. The relationship between donor BMI and clinical pregnancies was assessed after adjusting for recipient BMI. Clinical pregnancy rates and live birth rates (L...
journal_title：Reproductive biomedicine online
pub_type： 杂志文章
doi：10.1016/j.rbmo.2017.07.019
更新日期：2017-12-01 00:00:00
abstract：:The uptake of prenatal diagnosis in 436 singleton and 146 twin pregnancies following assisted reproduction was compared with a matched group of spontaneous conceptions. The first and second trimester ultrasound examination included target fetal anatomic evaluation and screening by specific markers described for fetal ...
journal_title：Reproductive biomedicine online
pub_type： 杂志文章
doi：10.1016/s1472-6483(10)60506-8
更新日期：2004-01-01 00:00:00
abstract：:Cleavage kinetics of human embryos is indicative of ability to develop to blastocyst and implant. Recent advances in time-lapse microscopy have opened new and important research opportunities. In this study involving infertile couples requiring standard IVF/intracytoplasmic sperm injection treatment, zygotes were cult...
journal_title：Reproductive biomedicine online
pub_type： 杂志文章
doi：10.1016/j.rbmo.2012.07.016
更新日期：2012-11-01 00:00:00
abstract：:The introduction to this review discusses briefly why immunology, perceived as difficult by assisted reproduction technology clinicians, need nevertheless be envisaged as a central actor in the reproduction process, and how the maternal immune system, initially perceived as a threat to the fetoplacental unit, is in fa...
journal_title：Reproductive biomedicine online
pub_type：
doi：10.1016/s1472-6483(10)60572-x
更新日期：2008-02-01 00:00:00
abstract：:The transvaginal approach to tubo-ovarian surgery allows easy and direct access to the tubo-ovarian structures and the fossa ovarica without additional manipulation. In the absence of a panoramic view, the proximity of the tubo-ovarian structures allows operative procedures for treatment of superficial and cystic ovar...
journal_title：Reproductive biomedicine online
pub_type： 杂志文章,评审
doi：10.1016/s1472-6483(12)60121-7
更新日期：2002-01-01 00:00:00
abstract：:In nature, HCG is secreted by the implanting embryo from peri-implantation and onwards. In contrast, LH is mandatory for steroidogenesis and follicular development during the follicular phase, working in synergy with FSH. Moreover, LH is mandatory for the function of the corpus luteum. Although LH and HCG bind to the ...
journal_title：Reproductive biomedicine online
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.rbmo.2017.05.001
更新日期：2017-08-01 00:00:00
abstract：:Reactive oxygen species (ROS) have a negative impact on sperm DNA, leading to the formation of oxidative products such as 8-oxo-7,8-dihydroxyguanosine. This compound causes fragmentation and, thus, has a mutagenic effect. Patient treatment with oral antioxidant vitamins is, therefore, standard practice for male infert...
journal_title：Reproductive biomedicine online
pub_type： 杂志文章,多中心研究
doi：10.1016/s1472-6483(10)60887-5
更新日期：2007-04-01 00:00:00
abstract：:Participation in external quality control (EQC) programmes is recommended by various scientific societies. Results from an EQC programme for embryology laboratories are presented. This 5-year programme consisted of the annual delivery of (i) materials to test toxicity and (ii) a DVD/CD-ROM with images of zygotes and e...
journal_title：Reproductive biomedicine online
pub_type： 杂志文章
doi：10.1016/j.rbmo.2009.09.033
更新日期：2010-01-01 00:00:00
abstract：:Case reports reveal that clinicians applying preimplantation genetic diagnosis are increasingly confronted with requests by patients which they consider ethically problematic. These requests raise the question to what extent physicians share responsibility for the welfare of the future child. Two categories of situati...
journal_title：Reproductive biomedicine online
pub_type： 杂志文章
doi：10.1016/s1472-6483(10)62068-8
更新日期：2003-11-01 00:00:00
abstract：:The discovery of cell-free fetal DNA in maternal plasma in 1997 has stimulated a rapid development of non-invasive prenatal testing. The recent advent of massively parallel sequencing has allowed the analysis of circulating cell-free fetal DNA to be performed with unprecedented sensitivity and precision. Fetal trisomi...
journal_title：Reproductive biomedicine online
pub_type： 杂志文章,评审
doi：10.1016/j.rbmo.2013.08.008
更新日期：2013-12-01 00:00:00
abstract：:In medicine the changes in therapies are the evidence of the progress in knowledge. But many examples concerning the treatment of early spontaneous abortion, late abortion, menopause, contraception and infertility show that gynaecologists and obstetricians change their treatments just as fashion designers change their...
journal_title：Reproductive biomedicine online
pub_type： 历史文章,杂志文章
doi：10.1016/s1472-6483(10)60903-0
更新日期：2007-04-01 00:00:00
abstract：:Epigenetic alterations at several maternal loci have been associated with imprinting disorders in children conceived using assisted reproductive technologies. To date, epimutations at paternal loci have been observed in the spermatozoa of infertile men, but there is little evidence of paternal epimutations in babies c...
journal_title：Reproductive biomedicine online
pub_type： 杂志文章
doi：10.1016/j.rbmo.2010.02.025
更新日期：2010-06-01 00:00:00
abstract：RESEARCH QUESTION:What is the relationship between the vaginal microbiota profile at the time of embryo transfer and live birth rates in women undergoing IVF/intracytoplasmic sperm injection (ICSI) with donated oocytes? DESIGN:One hundred and fifty Caucasian women receiving donated oocytes were prospectively included ...
journal_title：Reproductive biomedicine online
pub_type： 杂志文章
doi：10.1016/j.rbmo.2018.12.019
更新日期：2019-06-01 00:00:00
abstract：RESEARCH QUESTION:Does music therapy help in reducing pain and anxiety in women undergoing transvaginal ultrasound-guided oocyte retrieval (TUGOR)? DESIGN:In this randomized controlled open label study, 209 participants were recruited and randomized into three groups (music group, n = 70; headphone group, n = 70; cont...
journal_title：Reproductive biomedicine online
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.rbmo.2018.04.049
更新日期：2018-08-01 00:00:00
abstract：:For some patients, the autotransplantation of a cryopreserved-thawed intact ovary might be the best option to preserve their reproductive potential after fertility-threatening treatment. The best procedure to successfully cryopreserve a human ovary without inflicting a devastating level of cryodamage is to date unknow...
journal_title：Reproductive biomedicine online
pub_type： 杂志文章
doi：10.1016/j.rbmo.2011.08.008
更新日期：2011-12-01 00:00:00
abstract：:Fertility-preservation techniques for medical reasons are increasingly offered in national networks. Knowledge of the characteristics of counselled patients and techniques used are essential. The FertiPROTEKT network registry was analysed between 2007 and 2013, and included up to 85 university and non-university centr...
journal_title：Reproductive biomedicine online
pub_type： 杂志文章
doi：10.1016/j.rbmo.2015.07.013
更新日期：2015-11-01 00:00:00
abstract：:FSH exists as a family of isohormones exhibiting distinct oligosaccharide structures, and the released FSH isoform mixtures change during the follicular phase of the menstrual cycle. In addition, the pulsatile release of gonadotrophins seems to expose follicles to bursts of more less-acidic FSH isoforms and the follic...
journal_title：Reproductive biomedicine online
pub_type： 杂志文章,评审
doi：10.1016/s1472-6483(10)61826-3
更新日期：2002-11-01 00:00:00
abstract：:The aim of this prospective, randomized clinical trial (RCT) was to evaluate whether the supplemental protein concentration in embryo transfer (ET) medium affects the clinical outcomes in IVF-ET. A total of 750 patients undergoing IVF-ET who met the inclusion criteria were randomly divided into three groups, according...
journal_title：Reproductive biomedicine online
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.rbmo.2015.10.004
更新日期：2016-01-01 00:00:00
abstract：:Gentle ovarian stimulation protocols, such as 'mini-IVF', have several potential advantages over conventional IVF protocols, including less medication and fewer injections, producing fewer eggs, but eggs of higher quality. The particular 'mild' stimulation protocol called 'mini-IVF' is described. This protocol require...
journal_title：Reproductive biomedicine online
pub_type： 杂志文章
doi：10.1016/j.rbmo.2010.06.033
更新日期：2010-10-01 00:00:00
abstract：:This study evaluated the cost-effectiveness of treatments for women with polycystic ovary syndrome (PCOS) who ovulate on clomiphene citrate but do not conceive after six cycles. A decision-analytic framework was developed for six scenarios: (1) three cycles of IVF; (2) continuation of clomiphene citrate for six cycles...
journal_title：Reproductive biomedicine online
pub_type： 杂志文章
doi：10.1016/j.rbmo.2014.01.014
更新日期：2014-05-01 00:00:00
abstract：RESEARCH QUESTION:The prevalence of infertility in couples actively trying to conceive is 25%. What is the consultation-seeking behaviour, diagnosis and related treatment in infertile couples across China? DESIGN:Large cross-sectional population-based study in 2010-2011, in which 25,270 couples from eight provinces/mu...
journal_title：Reproductive biomedicine online
pub_type： 杂志文章
doi：10.1016/j.rbmo.2018.11.034
更新日期：2019-06-01 00:00:00
abstract：:The population attempting pregnancy and having babies is ageing. Gynaecological and obstetric complications worsen with age. Maternity services are struggling. Increasing rates of infertility and complications are not matched by the marvels in the laboratory. This paper argues that assisted reproduction treatment has ...
journal_title：Reproductive biomedicine online
pub_type： 杂志文章,评审
doi：10.1016/j.rbmo.2013.09.012
更新日期：2013-12-01 00:00:00
