Abstract：
:The effect of freeze-thaw (FT) cycles on Arctic tundra soil bacterial community was studied in laboratory microcosms. FT-induced changes to the bacterial community were followed over a 60-day period by terminal restriction fragment length polymorphism (T-RFLP) profiles of amplified 16S rRNA genes and reverse transcribed 16S rRNA. The main phylotypes of the active, RNA-derived bacterial community were identified using clone analysis. Non-metric multidimensional scaling ordination of the T-RFLP profiles indicated some shifts in the bacterial communities after three to five FT cycles at -2, -5, and -10 degrees C as analyzed both from the DNA and rRNA. The dominating T-RFLP peaks remained the same, however, and only slight variation was generally detected in the relative abundance of the main T-RF sizes of either DNA or rRNA. T-RFLP analysis coupled to clone analysis of reverse transcribed 16S rRNA indicated that the initial soil was dominated by members of Bacteroidetes, Acidobacteria, Alpha-, Beta-, and Gammaproteobacteria. The most notable change in the rRNA-derived bacterial community was a decrease in the relative abundance of a Betaproteobacteria-related phylotype after the FT cycles. This phylotype decreased, however, also in the control soil incubated at constant +5 degrees C suggesting that the decrease was not directly related to FT sensitivity. The results indicate that FT caused only minor changes in the bacterial community structure.
journal_name
Microb Ecoljournal_title
Microbial ecologyauthors
Männistö MK,Tiirola M,Häggblom MMdoi
10.1007/s00248-009-9516-xsubject
Has Abstractpub_date
2009-10-01 00:00:00pages
621-31issue
3eissn
0095-3628issn
1432-184Xjournal_volume
58pub_type
杂志文章abstract：:Maize root colonization and phosphate solubilizing activity of the fungus Penicillium rugulosum were assessed in a greenhouse trial using soil-plant microcosms. The bacterial gene hph conferring resistance to hygromicin B was introduced by electroporation in the wild-type strain IR-94MF1 of P. rugulosum and one transf...
journal_title：Microbial ecology
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doi：10.1007/s00248-002-1001-8
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abstract：:The recovery of bdellovibrios from estuarine sediments over an annual cycle was studied. Greater numbers of the predators were recovered in sediment than in the water column. Increases in the number of bdellovibrios recovered from sediment over various periods of time suggest that multiplication of the predators occur...
journal_title：Microbial ecology
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journal_title：Microbial ecology
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doi：10.1007/s00248-006-9009-0
更新日期：2007-01-01 00:00:00
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doi：10.1007/s00248-005-0247-3
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journal_title：Microbial ecology
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abstract：:Biotic and abiotic factors can influence interactions between microbial grazers and their prey, thus impacting both the cycling of biogenic carbon within the surface layer of the ocean and the export of carbon to the deep ocean and higher trophic levels. In this study, microcosm experiments were used to evaluate the c...
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journal_title：Microbial ecology
pub_type： 杂志文章
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更新日期：2001-08-01 00:00:00
abstract：:The beef cattle industry represents a significant portion of the USA's agricultural sect, with beef cattle accounting for the most red meat consumed in the USA. Feed represents the largest input cost in the beef industry, accounting for approximately 70% of total input cost. Given that, novel methods need to be employ...
journal_title：Microbial ecology
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更新日期：2019-11-01 00:00:00
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journal_title：Microbial ecology
pub_type： 杂志文章
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更新日期：2009-04-01 00:00:00
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journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/BF02020340
更新日期：1980-12-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/BF02539143
更新日期：1991-12-01 00:00:00
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journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-012-0062-6
更新日期：2012-10-01 00:00:00
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journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/BF02018912
更新日期：1988-09-01 00:00:00
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journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-007-9216-3
更新日期：2007-10-01 00:00:00
abstract：:Biofilms, microbial mats, and microbialites dwell under highly limiting conditions (high salinity, extreme aridity, pH, and elevated arsenic concentration) in the Andean Puna. Only recent pioneering studies have described the microbial diversity of different Altiplano lakes and revealed their unexpectedly diverse micr...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-018-1159-3
更新日期：2018-10-01 00:00:00
abstract：:A survey was made of the yeast communities isolated from necrotic tissue of 4 species of prickly-pear cacti (Opuntia stricta, O. tomentosa, O. monacantha, andO. streptacantha) which have colonized in Australia. Yeast communities were sampled from a number of localities and at different times. Cactus specific yeasts ac...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/BF02015562
更新日期：1984-12-01 00:00:00
abstract：:We demonstrated previously that Cr(VI) is readily reduced to oxoCr(V)-diols at the surface of Arthrobacter oxydans-a Gram-positive aerobic bacteria isolated from Columbia basalt rocks originated from a highly contaminated site in the USA. Here, we report an electron spin resonance (ESR) study of Cr(III) hydroxide form...
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更新日期：2009-02-01 00:00:00
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更新日期：2017-01-01 00:00:00
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更新日期：2012-08-01 00:00:00
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更新日期：1989-09-01 00:00:00
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更新日期：2020-02-01 00:00:00
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journal_title：Microbial ecology
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更新日期：1981-03-01 00:00:00
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更新日期：2004-07-01 00:00:00
abstract：:Lasallia hispanica, Parmelia omphalodes, and Cornicularia normoerica, saxicolous thalli growing on granite, show a close relationship with other lichens and microorganisms living in the lithic substrate beneath them. The lithobiontic community is an accumulation of microorganisms at an interface forming a biofilm, whi...
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更新日期：2002-01-01 00:00:00
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更新日期：2021-01-01 00:00:00
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journal_title：Microbial ecology
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更新日期：1998-11-01 00:00:00
abstract：:The phospholipid fatty acid (PLFA) profiles of soil microbial communities were determined in relation to the patterns of tree cover in a mature Norway spruce plantation. Replicate samples of the surface organic layers were taken close to the trunk, at 1 m and at 2 m (under the edge of the canopy) beneath five trees. S...
journal_title：Microbial ecology
pub_type： 杂志文章
doi：10.1007/s00248-001-0006-z
更新日期：2001-10-01 00:00:00
