Rock phosphate solubilization and colonization of maize rhizosphere by wild and genetically modified strains of Penicillium rugulosum.


:Maize root colonization and phosphate solubilizing activity of the fungus Penicillium rugulosum were assessed in a greenhouse trial using soil-plant microcosms. The bacterial gene hph conferring resistance to hygromicin B was introduced by electroporation in the wild-type strain IR-94MF1 of P. rugulosum and one transformant, w-T3, was selected. Maize plants were grown for 5 weeks in a P-poor soil and fertilized with a Florida apatite mineral, with Navay, an apatite rock deposit from Venezuela, or with simple superphosphate. Inoculation treatments included strain IR-94MF1, transformant w-T3 and two IR-94MF1 UV-induced mutants with enhanced (Mps++) or reduced (Mps-) in vitro mineral phosphate solubilizing activity. In the absence of P fertilization, inoculation with any P. rugulosum isolate significantly reduced the size of the total and P-solubilizing bacterial community present in maize rhizosphere. The bacterial community significantly increased in maize inoculated with IR-94MF1 and w-T3 when P was added as apatites Navay or Florida. All P. rugulosum strains were able to stimulate the growth of maize plants as indicated by 3.6 to 28.6% increases in dry matter yields. In the presence of rock phosphate, P uptake by maize plants inoculated with the two mutants Mps++ and Mps- was not always in agreement with their P-solubilizing phenotypes. Strain IR-94MF1 and transformant w-T3 increased P assimilation by the plants fertilized with Navay rock phosphate by 26 and 38%, respectively. In this treatment, w-T3 showed its highest significant maize rhizosphere colonization. With the simple superphosphate treatment, w-T3 increased P uptake in plants by 8% over the uninoculated control and also decreased significantly the community size of total bacteria, total fungi, and P-solubilizing fungi in the rhizosphere.


Microb Ecol


Microbial ecology


Reyes I,Bernier L,Antoun H





Has Abstract


2002-07-01 00:00:00












  • Multiple, novel biologically active endophytic actinomycetes isolated from upper Amazonian rainforests.

    abstract::Microbial biodiversity provides an increasingly important source of medically and industrially useful compounds. We have isolated 14 actinomycete species from a collection of approximately 300 plant stem samples from the upper Amazonian rainforest in Peru. All of the cultured isolates produce substances with inhibitor...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Bascom-Slack CA,Ma C,Moore E,Babbs B,Fenn K,Greene JS,Hann BD,Keehner J,Kelley-Swift EG,Kembaiyan V,Lee SJ,Li P,Light DY,Lin EH,Schorn MA,Vekhter D,Boulanger LA,Hess WM,Vargas PN,Strobel GA,Strobel SA

    更新日期:2009-08-01 00:00:00

  • An evaluation of 18S rDNA approaches for the study of fungal diversity in grassland soils.

    abstract::Fungal community structure and diversity in two types of agricultural grassland soil were investigated by amplified 18S ribosomal DNA restriction analysis (ARDRA) and 18S ribosomal DNA sequence analysis. These two grassland sites represent a species-rich old hay meadow and an agriculturally improved site with low flor...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Hunt J,Boddy L,Randerson PF,Rogers HJ

    更新日期:2004-05-01 00:00:00

  • The use of phospholipid fatty acid analysis to measure impact of acid rock drainage on microbial communities in sediments.

    abstract::The impact of acid rock drainage (ARD) and eutrophication on microbial communities in stream sediments above and below an abandoned mine site in the Adelaide Hills, South Australia, was quantified by PLFA analysis. Multivariate analysis of water quality parameters, including anions, soluble heavy metals, pH, and condu...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Ben-David EA,Holden PJ,Stone DJ,Harch BD,Foster LJ

    更新日期:2004-10-01 00:00:00

  • Epidemic mortality of the sponge Ircinia variabilis (Schmidt, 1862) associated to proliferation of a Vibrio bacterium.

    abstract::In recent years, several episodes of mass mortality of sessile epibenthic invertebrates, including sponges, have been recorded worldwide. In the present study, we report a disease event on Ircinia variabilis recorded in September 2009 along the southern Adriatic and Ionian seas (Apulian coast), with the aim to quantif...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Stabili L,Cardone F,Alifano P,Tredici SM,Piraino S,Corriero G,Gaino E

    更新日期:2012-10-01 00:00:00

  • Temperature effects on steady-state growth, phosphorus uptake, and the chemical composition of a marine phytoplankter.

    abstract::The marine chrysophyteMonochrysis lutheri was grown in phosphorus-limited continuous cultures at temperatures of 15°, 18.8° and 23°C. The effect of temperature on the maximum growth rate was well-defined by the Arrhenius equation, but the Q10 for this alga (1.7) was somewhat lower than has been determined previously f...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Goldman JC

    更新日期:1979-09-01 00:00:00

  • Molecular Characterisation and Co-cultivation of Bacterial Biofilm Communities Associated with the Mat-Forming Diatom Didymosphenia geminata.

    abstract::Didymosphenia geminata (Lyngbye) M. Schmidt is a stalked freshwater diatom that is expanding its range globally. In some rivers, D. geminata forms thick and expansive polysaccharide-dominated mats. Like other stalked diatoms, D. geminata cells attach to the substratum with a pad of adhesive extracellular polymeric sub...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Brandes J,Kuhajek JM,Goodwin E,Wood SA

    更新日期:2016-10-01 00:00:00

  • Altering the Gut Microbiome of Cattle: Considerations of Host-Microbiome Interactions for Persistent Microbiome Manipulation.

    abstract::The beef cattle industry represents a significant portion of the USA's agricultural sect, with beef cattle accounting for the most red meat consumed in the USA. Feed represents the largest input cost in the beef industry, accounting for approximately 70% of total input cost. Given that, novel methods need to be employ...

    journal_title:Microbial ecology

    pub_type: 杂志文章,评审


    authors: Clemmons BA,Voy BH,Myer PR

    更新日期:2019-02-01 00:00:00

  • Changes in soil bacterial community structure with increasing disturbance frequency.

    abstract::Little is known of the responsiveness of soil bacterial community structure to disturbance. In this study, we subjected a soil microcosm to physical disturbance, sterilizing 90 % of the soil volume each time, at a range of frequencies. We analysed the bacterial community structure using 454 pyrosequencing of the 16S r...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Kim M,Heo E,Kang H,Adams J

    更新日期:2013-07-01 00:00:00

  • Bacterial Diversity in Bentonites, Engineered Barrier for Deep Geological Disposal of Radioactive Wastes.

    abstract::The long-term disposal of radioactive wastes in a deep geological repository is the accepted international solution for the treatment and management of these special residues. The microbial community of the selected host rocks and engineered barriers for the deep geological repository may affect the performance and th...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Lopez-Fernandez M,Cherkouk A,Vilchez-Vargas R,Jauregui R,Pieper D,Boon N,Sanchez-Castro I,Merroun ML

    更新日期:2015-11-01 00:00:00

  • Effects of Carbon Source, Carbon Concentration, and Chlorination on Growth Related Parameters of Heterotrophic Biofilm Bacteria.

    abstract::To investigate growth of heterotrophic biofilm bacteria, a model biofilm reactor was developed to simulate a drinking water distribution system. Controlled addition of three different carbon sources (amino acids, carbohydrates, and humics) at three different concentrations (500, 1,000, and 2,000 ppb carbon) in the pre...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Ellis BD,Butterfield P,Jones WL,McFeters GA,Camper AK

    更新日期:1999-11-01 00:00:00

  • Emergence of a Synergistic Diversity as a Response to Competition in Pseudomonas putida Biofilms.

    abstract::Genetic diversification through the emergence of variants is one of the known mechanisms enabling the adaptation of bacterial communities. We focused in this work on the adaptation of the model strain Pseudomonas putida KT2440 in association with another P. putida strain (PCL1480) recently isolated from soil to invest...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Bridier A,Piard JC,Briandet R,Bouchez T

    更新日期:2020-07-01 00:00:00

  • Regulation of Hydrolytic Enzyme Activity in Aquatic Microbial Communities Hosted by Carnivorous Pitcher Plants.

    abstract::Carnivorous pitcher plants Sarracenia purpurea host diverse eukaryotic and bacterial communities which aid in insect prey digestion, but little is known about the functional processes mediated by the microbial communities. This study aimed to connect pitcher community diversity with functional nutrient transformation ...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Young EB,Sielicki J,Grothjan JJ

    更新日期:2018-11-01 00:00:00

  • Dynamics of seawater bacterial communities in a shellfish hatchery.

    abstract::Bacterial disease is a significant issue for larviculture of several species of shellfish, including oysters. One source of bacteria is the seawater used throughout the hatchery. In this study carried out at a commercial oyster hatchery in Tasmania, Australia, the diversity of the bacterial community and its relations...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Powell SM,Chapman CC,Bermudes M,Tamplin ML

    更新日期:2013-08-01 00:00:00

  • Pigmented nanoflagellates grazing on Synechococcus: seasonal variations and effect of flagellate size in the coastal ecosystem of subtropical Western Pacific.

    abstract::We investigated seasonal variation of grazing impact of the pigmented nanoflagellates (PNF) with different sizes upon Synechococcus in the subtropical western Pacific coastal waters using grazing experiments with fluorescently labeled Synechococcus (FLS). For total PNF, conspicuous seasonal variations of ingestion rat...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Chan YF,Tsai AY,Chiang KP,Hsieh CH

    更新日期:2009-10-01 00:00:00

  • Seasonal Variability May Affect Microbial Decomposers and Leaf Decomposition More Than Warming in Streams.

    abstract::Ongoing climate change is expected to affect the diversity and activity of aquatic microbes, which play a key role in plant litter decomposition in forest streams. We used a before-after control-impact (BACI) design to study the effects of warming on a forest stream reach. The stream reach was divided by a longitudina...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Duarte S,Cássio F,Ferreira V,Canhoto C,Pascoal C

    更新日期:2016-08-01 00:00:00

  • A study of the occurrence and distribution of bdellovibrios in estuarine sediment over an annual cycle.

    abstract::The recovery of bdellovibrios from estuarine sediments over an annual cycle was studied. Greater numbers of the predators were recovered in sediment than in the water column. Increases in the number of bdellovibrios recovered from sediment over various periods of time suggest that multiplication of the predators occur...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Williams HN

    更新日期:1988-01-01 00:00:00

  • Viable real-time PCR in environmental samples: can all data be interpreted directly?

    abstract::Selective nucleic acid intercalating dyes--ethidium monoazide (EMA) and propidium monoazide (PMA)--represent one of the most successful recent approaches to detect viable cells (as defined by an intact cell membrane) by PCR and have been effectively evaluated in different microorganisms. However, some practical limita...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Fittipaldi M,Codony F,Adrados B,Camper AK,Morató J

    更新日期:2011-01-01 00:00:00

  • Patterns of Multi-Antibiotic-Resistant Escherichia Coli from Streams with No History of Antimicrobial Inputs.

    abstract::A growing body of evidence suggests that contaminated environments may harbor a greater proportion of antibiotic-resistant microorganisms than unpolluted reference sites. Here, we report the screening of 427 Escherichia coli strains isolated from 11 locations on nine streams draining the US Department of Energy's Sava...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: McArthur JV,Fletcher DE,Tuckfield RC,Baker-Austin C

    更新日期:2016-11-01 00:00:00

  • Bacterial Stimulation in Mixed Cultures of Bacteria and Organic Carbon from River and Lake Waters.

    abstract::Abstract Interactions between natural bacterial assemblages and dissolved organic carbon (DOC) were investigated in two complementary batch experiments. In the first, a positive relationship was found between the proportion of electron transport system (ETS) active bacteria and the diversity of DOC in microcosms enric...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Gayte X,Fontvieille D,Wilkinson KJ

    更新日期:1999-10-01 00:00:00

  • Evidence of increased diversity of methanogenic archaea with plant extract supplementation.

    abstract::This study evaluated the effects of selected essential oils on archaeal communities using the ovine rumen model. Forty weaned Canadian Arcott ewes, fed with barley-based diet, were allotted to one of three essential oil supplementation treatments or a control (10 ewes per treatment) for 13 weeks. The treatments were c...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Ohene-Adjei S,Chaves AV,McAllister TA,Benchaar C,Teather RM,Forster RJ

    更新日期:2008-08-01 00:00:00

  • Resilience of Fungal Communities to Elevated CO2.

    abstract::Soil filamentous fungi play a prominent role in regulating ecosystem functioning in terrestrial ecosystems. This necessitates understanding their responses to climate change drivers in order to predict how nutrient cycling and ecosystem services will be influenced in the future. Here, we provide a quantitative synthes...

    journal_title:Microbial ecology

    pub_type: 杂志文章,meta分析


    authors: Veresoglou SD,Anderson IC,de Sousa NM,Hempel S,Rillig MC

    更新日期:2016-08-01 00:00:00

  • Distribution of exopolymeric substances in the littoral sediments of an oligotrophic lake.

    abstract::Bacteria and algae release exopolymeric substances (EPS) that perform a wide range of important functions in aquatic and terrestrial systems. In this study we measured EPS in sediments at nine littoral sites around a shallow oligotrophic basin, and tested whether the concentration and composition of EPS was related to...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Hirst CN,Cyr H,Jordan IA

    更新日期:2003-07-01 00:00:00

  • Bacterial abundance, activity, and viability in the eutrophic River Warnow, northeast Germany.

    abstract::The River Warnow is the drinking water source for the city of Rostock. Its eutrophic status is accompanied by high amounts of bacteria, which may reach up to 24 x 10(6) cells mL(-1) as recorded during a seasonal study in 2002. Because the river is eutrophic and also heavily loaded with organic matter, this burden is a...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Freese HM,Karsten U,Schumann R

    更新日期:2006-01-01 00:00:00

  • Detrital floc and surface soil microbial biomarker responses to active management of the nutrient impacted Florida everglades.

    abstract::Alterations in microbial community composition, biomass, and function in the Florida Everglades impacted by cultural eutrophication reflect a new physicochemical environment associated with monotypic stands of Typha domingensis. Phospholipid fatty acid (PLFA) biomarkers were used to quantify microbial responses in det...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Bellinger BJ,Hagerthey SE,Newman S,Cook MI

    更新日期:2012-11-01 00:00:00

  • Geographical Patterns of nirS Gene Abundance and nirS-Type Denitrifying Bacterial Community Associated with Activated Sludge from Different Wastewater Treatment Plants.

    abstract::Denitrifying bacteria is a driver of nitrogen removal process in wastewater treatment ecosystem. However, the geographical characteristics of denitrifying bacterial communities associated with activated sludge from diverse wastewater treatment plants (WWTPs) are still unclear. Here, quantitative PCR and next-generatio...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Zhang H,Feng J,Chen S,Zhao Z,Li B,Wang Y,Jia J,Li S,Wang Y,Yan M,Lu K,Hao H

    更新日期:2019-02-01 00:00:00

  • Coupling Bacterial Activity Measurements with Cell Sorting by Flow Cytometry.

    abstract::> Abstract A new procedure to investigate the relationship between bacterial cell size and activity at the cellular level has been developed; it is based on the coupling of radioactive labeling of bacterial cells and cell sorting by flow cytometry after SYTO 13 staining. Before sorting, bacterial cells were incubated ...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Servais P,Courties C,Lebaron P,Troussellier M

    更新日期:1999-08-01 00:00:00

  • Development and structure of eukaryotic biofilms in an extreme acidic environment, rio tinto (SW, Spain).

    abstract::An in situ colonization assay was performed to study the early stages of biofilm formation in Rio Tinto (SW, Spain), an extremely acidic environment (pH ca. 2). Eukaryotic assemblages were monitored at monthly intervals for 1 year. Diversity, colonization rates, and seasonal variations were analyzed. Structural featur...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Aguilera A,Souza-Egipsy V,Gómez F,Amils R

    更新日期:2007-02-01 00:00:00

  • PAH degradation capacity of soil microbial communities--does it depend on PAH exposure?

    abstract::Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous pollutants of the environment. But is their microbial degradation equally wide in distribution? We estimated the PAH degradation capacity of 13 soils ranging from pristine locations (total PAHs approximately 0.1 mg kg(-1)) to heavily polluted industrial sites (tot...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Johnsen AR,Karlson U

    更新日期:2005-11-01 00:00:00

  • Simultaneous growth on citrate reduces the effects of iron limitation during toluene degradation in Pseudomonas.

    abstract::Rhizoremediation has been suggested as an attractive bioremediation strategy for the effective breakdown of pollutants in soil. The presence of plant root exudates such as organic acids, sugars, and amino acids that may serve as carbon sources or biosynthetic building blocks and the limited bioavailability of iron may...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Dinkla IJ,Janssen DB

    更新日期:2003-01-01 00:00:00

  • Isolation, Characterization, and Metal Response of Novel, Acid-Tolerant Penicillium spp. from Extremely Metal-Rich Waters at a Mining Site in Transbaikal (Siberia, Russia).

    abstract::The role of fungi in metal cycling in acidic environments has been little explored to date. In this study, two acid-tolerant and metal-resistant Penicillium isolates, strains ShG4B and ShG4C, were isolated from a mine site in the Transbaikal area of Siberia (Russia). Waters at the mine site were characterized by extre...

    journal_title:Microbial ecology

    pub_type: 杂志文章


    authors: Glukhova LB,Frank YA,Danilova EV,Avakyan MR,Banks D,Tuovinen OH,Karnachuk OV

    更新日期:2018-11-01 00:00:00