Abstract：
BACKGROUND:Microbial communities are involved in many processes relevant to industrial and medical biotechnology, such as the formation of biofilms, lignocellulosic degradation, and hydrogen production. The manipulation of synthetic and natural microbial communities and their underlying ecological parameters, such as fitness, evolvability, and variation, is an increasingly important area of research for synthetic biology. RESULTS:Here, we explored how synthetic control of an endogenous circuit can be used to regulate a tradeoff between fitness in resource abundant and resource limited environments in a population of Saccharomyces cerevisiae. We found that noise in the expression of a key enzyme in ammonia assimilation, Gdh1p, mediated a tradeoff between growth in low nitrogen environments and stress resistance in high ammonia environments. We implemented synthetic control of an endogenous Gdh1p regulatory network to construct an engineered strain in which the fitness of the population was tunable in response to an exogenously-added small molecule across a range of ammonia environments. CONCLUSION:The ability to tune fitness and biological tradeoffs will be important components of future efforts to engineer microbial communities.
journal_name
J Biol Engjournal_title
Journal of biological engineeringauthors
Bayer TS,Hoff KG,Beisel CL,Lee JJ,Smolke CDdoi
10.1186/1754-1611-3-1subject
Has Abstractpub_date
2009-01-02 00:00:00pages
1issn
1754-1611pii
1754-1611-3-1journal_volume
3pub_type
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pub_type： 杂志文章
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pub_type： 杂志文章,评审
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journal_title：Journal of biological engineering
pub_type： 杂志文章
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更新日期：2012-07-23 00:00:00
