Abstract：
Background:Silkworm genetic engineering is widely used in gene function, silk engineering and disease-resistant engineering in most of Asia. Some of the earliest promoter elements are used to control the development of silkworm transgenic expression and gene therapy. However, the low expression and specificity of natural promoters limit the applications of genetic engineering. To construct a highly efficient synthetic inducible promoter in the Bombyx mori (Lepidoptera), we analyzed the regulatory elements and functional regions of the B. mori nucleopolyhedrovirus 39 K promoter. Results:Truncated mutation analysis of the 39 K promoter showed that the transcriptional regulatory region spanning positions - 573 to - 274 and + 1 to + 62 are essential for virus-inducible promoter activity. Further investigations using the electrophoretic mobility shift assay revealed that the baculovirus IE-1 protein binds to the 39 K promoter at the - 310 to - 355 region, and transcription activates the expression of 39 K promoter assay. Finally, we successfully constructed a synthetic inducible promoter that increased the virus-inducing activity of other promoters using the baculovirus-inducible transcriptional activation region that binds to specific core elements of 39 K (i.e., spanning the region - 310 to - 355). Conclusions:In summary, we constructed a novel, synthetic, and highly efficient biological tool, namely, a virus-inducible 39 K promoter, which provides endless possibilities for future research on gene function, gene therapy, and pest control in genetic engineering.
journal_name
J Biol Engjournal_title
Journal of biological engineeringauthors
Dong ZQ,Hu ZG,Li HQ,Jiang YM,Cao MY,Chen P,Lu C,Pan MHdoi
10.1186/s13036-018-0121-8subject
Has Abstractpub_date
2018-12-04 00:00:00pages
30issn
1754-1611pii
121journal_volume
12pub_type
杂志文章abstract：Background:Anti-CRISPR proteins are expressed by phages as a reaction to the bacterial CRISPR-Cas defense system. Recently, the structures of anti-CRISPR proteins have been determined, and their diverse functions have been clarified. Anti-CRISPR proteins such as LmAcrIIA2 and LmAcrIIA4 interact with the SpCas9:gRNA sys...
journal_title：Journal of biological engineering
pub_type： 杂志文章
doi：10.1186/s13036-018-0101-z
更新日期：2018-08-01 00:00:00
abstract：Background:This article explains some simple experiments that can be used in undergraduate or graduate physics or biomedical engineering laboratory classes to learn how birdcage volume radiofrequency (RF) coils and magnetic resonance imaging (MRI) work. For a clear picture, and to do any quantitative MRI analysis, acqu...
journal_title：Journal of biological engineering
pub_type： 杂志文章
doi：10.1186/s13036-017-0084-1
更新日期：2017-11-02 00:00:00
abstract：BACKGROUND:In antibody purification processes, the acidic buffer commonly used to elute the bound antibodies during conventional affinity chromatograph, can damage the antibody. Herein we describe the development of several types of affinity ligands which enable the purification of antibodies under much milder conditio...
journal_title：Journal of biological engineering
pub_type： 杂志文章
doi：10.1186/1754-1611-8-15
更新日期：2014-07-01 00:00:00
abstract：:The ovarian follicle is the structural and functional unit of the ovary, composed of the female gamete (the oocyte) and supportive somatic cells. Follicles are not only the source of a female's germ cell supply, but also secrete important hormones necessary for proper endocrine function. Folliculogenesis, the growth a...
journal_title：Journal of biological engineering
pub_type： 杂志文章,评审
doi：10.1186/s13036-018-0134-3
更新日期：2019-01-09 00:00:00
abstract：BACKGROUND:Among disposable bioreactor systems, cylindrical orbitally shaken bioreactors show important advantages. They provide a well-defined hydrodynamic flow combined with excellent mixing and oxygen transfer for mammalian and plant cell cultivations. Since there is no known universal correlation between the volume...
journal_title：Journal of biological engineering
pub_type： 杂志文章
doi：10.1186/1754-1611-7-28
更新日期：2013-12-02 00:00:00
abstract：Background:UP elements (upstream element) are DNA sequences upstream of a promoter that interact with the α-subunit of RNA polymerase (RNAP) and can affect transcription by altering the binding RNAP to DNA. However, details of UP element and binding affinity effects on transcriptional strength are unclear. Results:Her...
journal_title：Journal of biological engineering
pub_type： 杂志文章
doi：10.1186/s13036-017-0075-2
更新日期：2017-11-01 00:00:00
abstract：Background:Mesenchymal stem cells (MSCs) are multipotent stem cells that can be isolated and expanded from many tissues, and are being investigated for use in cell therapies. Though MSC therapies have demonstrated some success, none have been FDA approved for clinical use. MSCs lose stemness ex vivo, decreasing therape...
journal_title：Journal of biological engineering
pub_type： 杂志文章,评审
doi：10.1186/s13036-019-0140-0
更新日期：2019-01-18 00:00:00
abstract：UNLABELLED: BACKGROUND:Rapid increase in multi-walled carbon nanotube (MWCNT) production for their industrial and biomedical applications has led to concerns over the effects of MWCNTs on human health and the environment. Both animal and in vitro studies have provided important findings about MWCNT-induced effects on ...
journal_title：Journal of biological engineering
pub_type： 杂志文章
doi：10.1186/1754-1611-6-22
更新日期：2012-11-13 00:00:00
abstract：: AC Biosusceptometry (ACB) was previously employed towards recording gastrointestinal motility. Our data show a reliable and successful evaluation of gastrointestinal transit of liquid and solid meals in rats, considering the methods scarcity and number of experiments needed to endorsement of drugs and medicinal plant...
journal_title：Journal of biological engineering
pub_type： 杂志文章
doi：10.1186/1754-1611-6-6
更新日期：2012-05-15 00:00:00
abstract：: The following essay was written by Mr. Alex Hatch, a junior undergraduate student majoring in Biological Engineering at Utah State University. Mr. Hatch submitted a 1000-1200 word essay to the 5th Annual Bioethics Contest sponsored by the Institute of Biological Engineering (IBE). A group of professionals in Biologic...
journal_title：Journal of biological engineering
pub_type： 杂志文章
doi：10.1186/1754-1611-4-7
更新日期：2010-06-09 00:00:00
abstract：:Due to steadily growing population and economic transitions in the more populous countries, renewable sources of energy are needed more than ever. Plant biomass as a raw source of bioenergy and biofuel products may meet the demand for sustainable energy; however, such plants typically compete with food crops, which sh...
journal_title：Journal of biological engineering
pub_type： 杂志文章,评审
doi：10.1186/s13036-017-0069-0
更新日期：2017-09-01 00:00:00
abstract：Background:Aptamers, single-stranded DNAs or RNAs, can be selected from a library containing random sequences using a method called Systematic Evolution of Ligands by EXponential Enrichment (SELEX). In SELEX, monitoring the enriching statuses of aptamer candidates during the process is a key step until today. Conformat...
journal_title：Journal of biological engineering
pub_type： 杂志文章
doi：10.1186/s13036-019-0223-y
更新日期：2020-01-13 00:00:00
abstract：:In situ tissue regeneration approach aims to exploit the body's own biological resources and reparative capability and recruit host cells by utilizing cell-instructive biomaterials. In order to immobilize and release bioactive factors in biomaterials, it is important to engineer the load effectiveness, release kinetic...
journal_title：Journal of biological engineering
pub_type： 杂志文章,评审
doi：10.1186/s13036-017-0058-3
更新日期：2017-06-29 00:00:00
abstract：Background:Myocardial infarction (MI) is a common cause of mortality in people. Mesenchymal stem cell (MSC) has been shown to exert therapeutic potential to treat myocardial infarction (MI). However, in patients with diabetes, the diabetic environment affected MSCs activity and could impair the efficacy of treatment. I...
journal_title：Journal of biological engineering
pub_type： 杂志文章
doi：10.1186/s13036-019-0163-6
更新日期：2019-05-30 00:00:00
abstract：:Hydrogels are a three-dimensional and crosslinked network of hydrophilic polymers. They can absorb a large amount of water or biological fluids, which leads to their swelling while maintaining their 3D structure without dissolving (Zhu and Marchant, Expert Rev Med Devices 8:607-626, 2011). Among the numerous polymers ...
journal_title：Journal of biological engineering
pub_type： 杂志文章,评审
doi：10.1186/s13036-020-0227-7
更新日期：2020-03-13 00:00:00
abstract：Background:Oleaginous fungi are efficient tools to convert agricultural waste streams into valuable components. The filamentous fungus Mucor circinelloides was cultivated in whey permeate, a byproduct from cheese production, to produce an oil-rich fungal biomass. Response surface methodology was used to optimize the fe...
journal_title：Journal of biological engineering
pub_type： 杂志文章
doi：10.1186/s13036-018-0116-5
更新日期：2018-11-14 00:00:00
abstract：:Detecting the arbitrary movements of fast-moving insects under field conditions is notoriously difficult because existing technologies are limited by issues of size, weight, range and cost. Here, we establish proof-of-concept for a prototype long-range, passive radio frequency identification (RFID) tagging system for ...
journal_title：Journal of biological engineering
pub_type： 杂志文章
doi：10.1186/s13036-019-0143-x
更新日期：2019-02-07 00:00:00
abstract：BACKGROUND:The use of in silico simulations as a basis for designing artificial biological systems (and experiments to characterize them) is one of the tangible differences between Synthetic Biology and "classical" Genetic Engineering. To this end, synthetic biologists have adopted approaches originating from the tradi...
journal_title：Journal of biological engineering
pub_type： 杂志文章
doi：10.1186/1754-1611-7-26
更新日期：2013-11-18 00:00:00
abstract：BACKGROUND:Microbial communities are involved in many processes relevant to industrial and medical biotechnology, such as the formation of biofilms, lignocellulosic degradation, and hydrogen production. The manipulation of synthetic and natural microbial communities and their underlying ecological parameters, such as f...
journal_title：Journal of biological engineering
pub_type： 杂志文章
doi：10.1186/1754-1611-3-1
更新日期：2009-01-02 00:00:00
abstract：BACKGROUND:As engineered biological systems become more complex, it is increasingly common to express multiple operons from different plasmids and inducible expression systems within a single host cell. Optimizing such systems often requires screening combinations of origins of replication, expression systems, and anti...
journal_title：Journal of biological engineering
pub_type： 杂志文章
doi：10.1186/1754-1611-5-12
更新日期：2011-09-20 00:00:00
abstract：: Two current technologies used in biosensor development are very promising: 1. The sol-gel process of making microporous glass at room temperature, and 2. Using a fluorescent compound that undergoes fluorescence quenching in response to a specific analyte. These technologies have been combined to produce an iron biose...
journal_title：Journal of biological engineering
pub_type： 杂志文章
doi：10.1186/1754-1611-5-4
更新日期：2011-05-10 00:00:00
abstract：BACKGROUND:Poly(γ-glutamic acid) (γ-PGA) is a biopolymer with many useful properties making it applicable for instance in food and skin care industries, in wastewater treatment, in biodegradable plastics or in the pharmaceutical industry. γ-PGA is usually produced microbially by different Bacillus spp. The produced γ-P...
journal_title：Journal of biological engineering
pub_type： 杂志文章
doi：10.1186/s13036-017-0065-4
更新日期：2017-07-12 00:00:00
abstract：BACKGROUND:Surface-enhanced Raman scattering (SERS) is a powerful light scattering technique that can be used for sensitive immunoassay development and cell labeling. A major obstacle to using SERS is the complexity of fabricating SERS probes since they require nanoscale characterization and optical uniformity. The lig...
journal_title：Journal of biological engineering
pub_type： 杂志文章
doi：10.1186/s13036-015-0023-y
更新日期：2016-01-07 00:00:00
abstract：BACKGROUND:Glucagon is a peptide hormone with many uses as a therapeutic agent, including the emergency treatment of hypoglycemia. Physical instability of glucagon in solution leads to problems with the manufacture, formulation, and delivery of this pharmaceutical product. Glucagon has been shown to aggregate and form ...
journal_title：Journal of biological engineering
pub_type： 杂志文章
doi：10.1186/1754-1611-2-10
更新日期：2008-07-09 00:00:00
abstract：Background:Inulinase can hydrolyze polyfructan into high-fructose syrups and fructoligosaccharides, which are widely used in food, the medical industry and the biorefinery of Jerusalem artichoke. In the present study, a recombinant exo-inulinase (rKcINU1), derived from Kluyveromyces cicerisporus CBS4857, was proven as ...
journal_title：Journal of biological engineering
pub_type： 杂志文章
doi：10.1186/s13036-019-0215-y
更新日期：2019-10-29 00:00:00
abstract：Background:The production of recombinant proteins in mammalian cell lines is one of the most important areas in biopharmaceutical industry. Viral transcriptional promoters are widely used to express recombinant proteins in mammalian cell lines. However, these promoters are susceptible to silencing, thus limiting protei...
journal_title：Journal of biological engineering
pub_type： 杂志文章
doi：10.1186/s13036-019-0187-y
更新日期：2019-06-28 00:00:00
abstract：UNLABELLED: BACKGROUND:Biohydrogen from cyanobacteria has attracted public interest due to its potential as a renewable energy carrier produced from solar energy and water. Anabaena siamensis TISTR 8012, a novel strain isolated from rice paddy field in Thailand, has been identified as a promising cyanobacterial strain...
journal_title：Journal of biological engineering
pub_type： 杂志文章
doi：10.1186/1754-1611-6-19
更新日期：2012-10-10 00:00:00
abstract：BACKGROUND:The Hamiltonian Path Problem asks whether there is a route in a directed graph from a beginning node to an ending node, visiting each node exactly once. The Hamiltonian Path Problem is NP complete, achieving surprising computational complexity with modest increases in size. This challenge has inspired resear...
journal_title：Journal of biological engineering
pub_type： 杂志文章
doi：10.1186/1754-1611-3-11
更新日期：2009-07-24 00:00:00
abstract：:The materials used in bone tissue engineering (BTE) have been advancing with each passing day. With the continuous development of nanomedicine, gold nanoparticles (GNPs), which are easy to be synthesized and functionalized, have attracted increasing attention. Recent years have witnessed this amazing material, i.e., G...
journal_title：Journal of biological engineering
pub_type： 杂志文章,评审
doi：10.1186/s13036-020-00236-3
更新日期：2020-05-06 00:00:00
abstract：Background:Previously we demonstrated that an entire bacterial operon (the PRN operon) is expressible in plants when driven by the Tomato -yellow-leaf-curl-virus (TYLCV) -derived universal vector IL-60.Petroleum-derived plastics are not degradable, and are therefore harmful to the environment. Fermentation of bacteria ...
journal_title：Journal of biological engineering
pub_type： 杂志文章
doi：10.1186/s13036-017-0062-7
更新日期：2017-11-21 00:00:00
