Organogenic nodule development in hop (Humulus lupulus L.): transcript and metabolic responses.

Abstract:

BACKGROUND:Hop (Humulus lupulus L.) is an economically important plant forming organogenic nodules which can be used for genetic transformation and micropropagation. We are interested in the mechanisms underlying reprogramming of cells through stress and hormone treatments. RESULTS:An integrated molecular and metabolomic approach was used to investigate global gene expression and metabolic responses during development of hop's organogenic nodules. Transcript profiling using a 3,324-cDNA clone array revealed differential regulation of 133 unigenes, classified into 11 functional categories. Several pathways seem to be determinant in organogenic nodule formation, namely defense and stress response, sugar and lipid metabolism, synthesis of secondary metabolites and hormone signaling. Metabolic profiling using 1H NMR spectroscopy associated to two-dimensional techniques showed the importance of metabolites related to oxidative stress response, lipid and sugar metabolism and secondary metabolism in organogenic nodule formation. CONCLUSION:The expression profile of genes pivotal for energy metabolism, together with metabolites profile, suggested that these morphogenic structures gain energy through a heterotrophic, transport-dependent and sugar-degrading anaerobic metabolism. Polyamines and auxins are likely to be involved in the regulation of expression of many genes related to organogenic nodule formation. These results represent substantial progress toward a better understanding of this complex developmental program and reveal novel information regarding morphogenesis in plants.

journal_name

BMC Genomics

journal_title

BMC genomics

authors

Fortes AM,Santos F,Choi YH,Silva MS,Figueiredo A,Sousa L,Pessoa F,Santos BA,Sebastiana M,Palme K,Malhó R,Verpoorte R,Pais MS

doi

10.1186/1471-2164-9-445

subject

Has Abstract

pub_date

2008-09-29 00:00:00

pages

445

issn

1471-2164

pii

1471-2164-9-445

journal_volume

9

pub_type

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