Reconstitution and analysis of the multienzyme Escherichia coli RNA degradosome.

abstract：:The pH dependence of the amide/solvent hydrogen exchange of individual amide groups in hen egg-white lysozyme has been studied by nuclear magnetic resonance spectroscopy. Lysozyme has been used here as a model for a globular protein to re-examine the hypothesis for the amide/solvent hydrogen exchange reaction proposed...

journal_title：Journal of molecular biology

authors： Pedersen TG,Thomsen NK,Andersen KV,Madsen JC,Poulsen FM

更新日期：1993-03-20 00:00:00

abstract：:Experimental approaches for the identification of functionally important regions on the surface of a protein involve mutagenesis, in which exposed residues are replaced one after another while the change in binding to other proteins or changes in activity are recorded. However, practical considerations limit the use o...

journal_title：Journal of molecular biology

authors： Armon A,Graur D,Ben-Tal N

更新日期：2001-03-16 00:00:00

abstract：:Residue-specific potentials between pairs of side-chains and pairs of side-chain-backbone interaction sites have been generated by collecting radial distribution data for 302 protein structures. Multiple atomic interactions have been utilized to enhance the specificity and smooth the distance-dependence of the potenti...

journal_title：Journal of molecular biology

authors： Bahar I,Jernigan RL

更新日期：1997-02-14 00:00:00

abstract：:Rhodanese is an ubiquitous enzyme that in vitro catalyses the transfer of a sulfur atom from suitable donors to nucleophilic acceptors by way of a double displacement mechanism. During the catalytic process the enzyme cycles between a sulfur-free and a persulfide-containing form, via formation of a persulfide linkage ...

journal_title：Journal of molecular biology

authors： Bordo D,Deriu D,Colnaghi R,Carpen A,Pagani S,Bolognesi M

更新日期：2000-05-12 00:00:00

abstract：:Proteinaceous serine proteinase inhibitors are widespread throughout the plant kingdom where they play an important role in protection against pests and pathogens. Here, we describe the isolation and characterisation of a novel 14 amino acid residue cyclic peptide from sunflower seeds, which is a potent inhibitor of t...

journal_title：Journal of molecular biology

authors： Luckett S,Garcia RS,Barker JJ,Konarev AV,Shewry PR,Clarke AR,Brady RL

更新日期：1999-07-09 00:00:00

abstract：:The integrase protein (Int) from bacteriophage lambda is the archetypal member of the tyrosine recombinase family, a large group of enzymes that rearrange DNA in all domains of life. Int catalyzes the insertion and excision of the viral genome into and out of the Escherichia coli chromosome. Recombination transpires w...

journal_title：Journal of molecular biology

authors： Fadeev EA,Sam MD,Clubb RT

更新日期：2009-05-15 00:00:00

abstract：:The steps of UUC recognition by tRNAPhe were analysed by temperature-jump measurements. At ion concentrations close to physiological conditions we found three relaxation processes, which we assigned to (1) formation of codon-anticodon complexes, (2) a conformational change of the anticodon loop coupled with Mg2+ bindi...

journal_title：Journal of molecular biology

authors： Labuda D,Striker G,Porschke D

更新日期：1984-04-25 00:00:00

abstract：:Bacterial cell shape is dictated by the cell wall, a plastic structure that must adapt to growth and division whilst retaining its function as a selectively permeable barrier. The modulation of cell wall structure is achieved by a variety of enzymatic functions, all of which must be spatially regulated in a precise ma...

journal_title：Journal of molecular biology

authors： Lovering AL,Strynadka NCJ

更新日期：2007-09-28 00:00:00

abstract：:In order to investigate the modes of DNA synthesis supported by the 66 and 51 kDa subunits of equine infectious anemia virus reverse transcriptase (EIAV RT), recombinant p66 polypeptides containing a modified ribonuclease H (RNase H) domain were purified and evaluated. Defined heteropolymeric template-primer combinati...

journal_title：Journal of molecular biology

authors： Rausch JW,Arts EJ,Wöhrl BM,Le Grice SF

更新日期：1996-04-05 00:00:00

abstract：:The fine folding and assembling characteristics of amyloid beta (Abeta) peptides are important to pharmaceutical studies of drug molecules and to the pathological analysis of neurodegenerative disorders such as Alzheimer's disease at the molecular level. Here we present observations of the multiple folding characteris...

journal_title：Journal of molecular biology

authors： Ma X,Liu L,Mao X,Niu L,Deng K,Wu W,Li Y,Yang Y,Wang C

更新日期：2009-05-15 00:00:00

abstract：:The 231-residue capsid (CA) protein of human immunodeficiency virus type 1 (HIV-1) spontaneously self-assembles into tubes with a hexagonal lattice that is believed to mimic the surface lattice of conical capsid cores within intact virions. We report the results of solid-state nuclear magnetic resonance (NMR) measurem...

journal_title：Journal of molecular biology

authors： Bayro MJ,Chen B,Yau WM,Tycko R

更新日期：2014-03-06 00:00:00

abstract：:At high concentrations, type I pN-collagen, pC-collagen and procollagen (the first 2 generated from procollagen by enzymic cleavage of C-propeptides and N-propeptides, respectively) can all be made to assemble in vitro into thin D-periodic sheets or tapes. Scanning transmission electron microscopy mass measurements sh...

journal_title：Journal of molecular biology

authors： Holmes DF,Mould AP,Chapman JA

更新日期：1991-07-05 00:00:00

abstract：:Sickle hemoglobin polymerization exhibits a striking sensitivity to initial concentration, with characteristic reaction times that exhibit 30th power dependence on concentration. This extraordinary reaction order is encompassed by a novel double nucleation mechanism that predicts 50th power dependence of the homogeneo...

journal_title：Journal of molecular biology

authors： Cao Z,Ferrone FA

更新日期：1996-02-23 00:00:00

abstract：:The structure of goose egg-white lysozyme (GEWL) has been refined to an R-value of 15.9% at 1.6 A resolution. Details of the structure determination, the refinement and the structure itself are presented. The structure of a complex of the enzyme with the trisaccharide of N-acetyl glucosamine has also been determined a...

journal_title：Journal of molecular biology

authors： Weaver LH,Grütter MG,Matthews BW

更新日期：1995-01-06 00:00:00

abstract：:The genome sciences face the challenge to characterize structure and function of a vast number of novel genes. Sequence search techniques are used to infer functional and structural information from similarities to experimentally characterized genes or proteins. The persistent goal is to refine these techniques and to...

journal_title：Journal of molecular biology

authors： Koppensteiner WA,Lackner P,Wiederstein M,Sippl MJ

更新日期：2000-03-03 00:00:00

abstract：:Human multi-domain-containing protein UHRF1 has recently been extensively characterized as a key epigenetic regulator for maintaining DNA methylation patterns. UHRF1 SRA domain preferentially binds to hemimethylated CpG sites, and double Tudor domain has been implicated in recognizing H3K9me3 mark, but the role of the...

journal_title：Journal of molecular biology

authors： Xie S,Jakoncic J,Qian C

更新日期：2012-01-13 00:00:00

abstract：:Catalytic and other functionally important residues in proteins can often be mutated to yield more stable proteins. Many of these residues are charged residues that are located in electrostatically unfavorable environments. Here it is demonstrated that because continuum electrostatics methods can identify these destab...

journal_title：Journal of molecular biology

authors： Elcock AH

更新日期：2001-09-28 00:00:00

abstract：:Factor H (FH) is a major complement control protein in serum. The seventh short complement regulator (SCR-7) domain of the 20 in FH is associated with age-related macular degeneration through a Tyr402His polymorphism. The recombinant SCR-6/8 domains containing either His402 or Tyr402 and their complexes with a heparin...

journal_title：Journal of molecular biology

authors： Fernando AN,Furtado PB,Clark SJ,Gilbert HE,Day AJ,Sim RB,Perkins SJ

更新日期：2007-04-27 00:00:00

abstract：:Steroids and retinoids are signaling molecules that control a variety of physiological processes. 17beta-Hydroxysteroid dehydrogenase type 1 (17beta-HSD1) catalyzes the reduction of estrone to estradiol, supplying biologically active estrogen-regulating sex-specific differentiation. Photoreceptor-associated retinol de...

journal_title：Journal of molecular biology

authors： Haller F,Moman E,Hartmann RW,Adamski J,Mindnich R

更新日期：2010-06-04 00:00:00

abstract：:We have studied the conditions needed for the formation of stable ternary complexes by Escherichia coli RNA polymerase using a procedure in which elongation by the majority of active enzyme molecules is halted at a specific template base. Stable complexes of this sort, containing RNA chains as short as 15 nucleotides,...

journal_title：Journal of molecular biology

authors： Levin JR,Krummel B,Chamberlin MJ

更新日期：1987-07-05 00:00:00

abstract：:A parallel stacking arrangement of the guanidinium groups of arginines directly over the center of the rings of aromatic side-chains is observed much more frequently in proteins than would be expected by chance. This type of interaction, which is often found in locations critical to the function, apparently serves to ...

journal_title：Journal of molecular biology

authors： Flocco MM,Mowbray SL

更新日期：1994-01-14 00:00:00

abstract：:Normal type I collagen is a heterotrimer of two alpha1(I) and one alpha2(I) chains, but various genetic and environmental factors result in synthesis of homotrimers that consist of three alpha1(I) chains. The homotrimers completely replace the heterotrimers only in rare recessive disorders. In the general population, ...

journal_title：Journal of molecular biology

authors： Han S,McBride DJ,Losert W,Leikin S

更新日期：2008-10-31 00:00:00

abstract：:Wild-type hen lysozyme has been converted from its soluble native state into highly organized amyloid fibrils. In order to achieve this conversion, conditions were chosen to promote partial unfolding of the native globular fold and included heating of low-pH solutions and addition of organic solvents. Two peptides der...

journal_title：Journal of molecular biology

authors： Krebs MR,Wilkins DK,Chung EW,Pitkeathly MC,Chamberlain AK,Zurdo J,Robinson CV,Dobson CM

更新日期：2000-07-14 00:00:00

abstract：:Riboflavin is an essential cofactor in all organisms. Its direct biosynthetic precursor, 6,7-dimethyl-8-ribityllumazine, is synthesised by the enzyme 6,7-dimethyl-8-ribityllumazine synthase. Recently, we have found that the enzyme from Schizosaccharomyces pombe binds riboflavin, the final product of the pathway with a...

journal_title：Journal of molecular biology

authors： Gerhardt S,Haase I,Steinbacher S,Kaiser JT,Cushman M,Bacher A,Huber R,Fischer M

更新日期：2002-05-17 00:00:00

abstract：:Despite proline being assumed to be a helix-breaker, a large number of alpha-helices are found to contain Pro in globular as well as membrane proteins. Proline has no free NH group and therefore cannot form the conventional intra-helical NH.O=C hydrogen bond. An analysis of known protein structures has shown that the ...

journal_title：Journal of molecular biology

authors： Chakrabarti P,Chakrabarti S

更新日期：1998-12-11 00:00:00

abstract：:Peptidyl-tRNA hydrolase cleaves the ester bond between tRNA and the attached peptide in peptidyl-tRNA in order to avoid the toxicity resulting from its accumulation and to free the tRNA available for further rounds in protein synthesis. The structure of the enzyme from Mycobacterium tuberculosis has been determined in...

journal_title：Journal of molecular biology

authors： Selvaraj M,Roy S,Singh NS,Sangeetha R,Varshney U,Vijayan M

更新日期：2007-09-07 00:00:00

abstract：:The properties have been determined of a recently identified form of bovine pancreatic trypsin inhibitor, with only two of the three disulphide bonds of the native protein, but possessing a native-like conformation. The kinetics of refolding of the reduced inhibitor were re-measured to elucidate the kinetic role in fo...

journal_title：Journal of molecular biology

authors： Creighton TE,Goldenberg DP

更新日期：1984-11-05 00:00:00

abstract：:Severe thermal stress induces massive intracellular protein aggregation. The concerted action of Hsp70 (DnaK, DnaJ, GrpE) and Hsp100 (ClpB) chaperones results in solubilization of aggregates followed by reactivation of proteins. It was shown that the Hsp70 chaperone system works at the initial step of the disaggregati...

journal_title：Journal of molecular biology

authors： Lewandowska A,Matuszewska M,Liberek K

更新日期：2007-08-17 00:00:00

abstract：:Bacteriophages are involved in many aspects of the spread and establishment of virulence factors in Staphylococcus aureus, including the mobilization of genetic elements known as S. aureus pathogenicity islands (SaPIs), which carry genes for superantigen toxins and other virulence factors. SaPIs are packaged into phag...

journal_title：Journal of molecular biology

authors： Spilman MS,Dearborn AD,Chang JR,Damle PK,Christie GE,Dokland T

更新日期：2011-01-21 00:00:00

abstract：:The activator protein-1 (AP-1) complex plays a crucial role in numerous pathways, and its ability to induce tumorigenesis is well documented. Thus, AP-1 represents an interesting therapeutic target. We selected peptides from phage display and compared their ability to disrupt the cFos/cJun interaction to a previously ...

journal_title：Journal of molecular biology

authors： Hagemann UB,Mason JM,Müller KM,Arndt KM

更新日期：2008-08-01 00:00:00