Amyloid beta (1-42) folding multiplicity and single-molecule binding behavior studied with STM.

abstract：:The Bacillus subtilis gnt operon is negatively regulated by the gnt repressor (GntR, 243 amino acids), which is antagonized by gluconate. The GntR protein belongs to a new family of bacterial regulatory proteins (GntR family). To locate the DNA-binding domain of the GntR protein, we obtained mutations of this protein,...

journal_title：Journal of molecular biology

authors： Yoshida K,Fujita Y,Sarai A

更新日期：1993-05-20 00:00:00

abstract：:An alphoid-like human repetitive DNA of the Sau3A family is present extrachromosomally and in the chromosomes. In the chromosomes, the DNA is located on chromosome 11 but related sequences are present in chromosome 17. We characterized the nature of the recombination involved in the excision of the extrachromosomal DN...

journal_title：Journal of molecular biology

authors： Kiyama R,Okumura K,Matsui H,Bruns GA,Kanda N,Oishi M

更新日期：1987-12-20 00:00:00

abstract：:Actin polymerization plays a major role in cell movement. The controls of actin sequestration/desequestration and of filament turnover are two important features of cell motility. Actin binding proteins use properties derived from the steady-state monomer-polymer cycle of actin in the presence of ATP, to control the F...

journal_title：Journal of molecular biology

authors： Carlier MF,Pantaloni D

更新日期：1997-06-20 00:00:00

abstract：:While tandem repeats of the human centromere DNA pentamer sequence TGRAA form stable "self-complementary" [TGRAATGRAA]2 duplexes (R = G or A) containing the GA-bracketed unpaired purine stack motif, their phase-shifted variants NAATGNAATG (N = A, G, C, T) were found to exist in solution as an equilibrium mixture of a ...

journal_title：Journal of molecular biology

authors： Chou SH,Zhu L,Reid BR

更新日期：1996-06-14 00:00:00

abstract：:In the filamentous fungus Podospora anserina, the unavoidable phenomenon of senescence is associated with the amplification of the first intron of the mitochondrial cox1 that accumulates as circular DNA molecules consisting of tandem repeats. This group II intron (cox1-i1 or alpha) is able to transpose and contains an...

journal_title：Journal of molecular biology

authors： Sainsard-Chanet A,Begel O,Belcour L

更新日期：1994-10-07 00:00:00

abstract：:Herpesvirus proteases are essential for the production of progeny virus. They cleave the assembly protein that fills the immature capsid in order to make place for the viral DNA. The recombinant protease of the human gamma-herpesvirus Epstein-Barr virus (EBV) was expressed in Escherichia coli and purified. Circular di...

journal_title：Journal of molecular biology

authors： Buisson M,Valette E,Hernandez JF,Baudin F,Ebel C,Morand P,Seigneurin JM,Arlaud GJ,Ruigrok RW

更新日期：2001-08-03 00:00:00

abstract：:The structure of the recombinant medium chain alcohol dehydrogenase (ADH) from the hyperthermophilic archaeon Aeropyrum pernix has been solved by the multiple anomalous dispersion technique using the signal from the naturally occurring zinc ions. The enzyme is a tetramer with 222 point group symmetry. The ADH monomer ...

journal_title：Journal of molecular biology

authors： Guy JE,Isupov MN,Littlechild JA

更新日期：2003-08-29 00:00:00

abstract：:The U6 RNA internal stem-loop (U6 ISL) is a highly conserved domain of the spliceosome that is important for pre-mRNA splicing. The U6 ISL contains an internal loop that is in equilibrium between two conformations controlled by the protonation state of an adenine (pK(a)=6.5). Lower pH favors formation of a protonated ...

journal_title：Journal of molecular biology

authors： Venditti V,Clos L 2nd,Niccolai N,Butcher SE

更新日期：2009-09-04 00:00:00

abstract：:This analysis takes an in-depth look into the difficulties encountered by automatic methods for domain decomposition from three-dimensional structure. The analysis involves a multi-faceted set of criteria including the integrity of secondary structure elements, the tendency toward fragmentation of domains, domain boun...

journal_title：Journal of molecular biology

authors： Holland TA,Veretnik S,Shindyalov IN,Bourne PE

更新日期：2006-08-18 00:00:00

abstract：:The three-dimensional crystal structures of the single mutant M17G and the triple mutant F14G-S15G-M17G of the response regulator protein CheY have been determined to 2.3 and 1.9 angstrom, respectively. Both mutants bind the essential Mg2+ cation as determined by the changes in stability, but binding does not cause th...

journal_title：Journal of molecular biology

authors： Bellsolell L,Cronet P,Majolero M,Serrano L,Coll M

更新日期：1996-03-22 00:00:00

abstract：:Rat genomic clones, which together contain all of the rat genomic gamma-crystallin sequences, have been characterized. Five gamma-crystallin genes are located on a contiguous DNA region, 63 X 10(3) base-pairs long. These genes, named (5') gamma 1-1, gamma 1-2, gamma 2-2 and gamma 3-1 (3'), are all oriented head to tai...

journal_title：Journal of molecular biology

authors： Moormann RJ,den Dunnen JT,Heuyerjans J,Jongbloed RJ,van Leen RW,Lubsen NH,Schoenmakers JG

更新日期：1985-04-05 00:00:00

abstract：:l-Rhamnose is a 6-deoxyhexose that is found in a variety of different glycoconjugates in the cell walls of pathogenic bacteria. The precursor of l-rhamnose is dTDP-l-rhamnose, which is synthesised from glucose- 1-phosphate and deoxythymidine triphosphate (dTTP) via a pathway requiring four enzymes. Significantly this ...

journal_title：Journal of molecular biology

authors： Allard ST,Giraud MF,Whitfield C,Graninger M,Messner P,Naismith JH

更新日期：2001-03-16 00:00:00

abstract：:The electrostatic interactions in the channels of OmpF and PhoE porins from Escherichia coli were analysed on the basis of a macroscopic multi-dielectric model of the protein-membrane complex derived from the respective porin X-ray structures. The membrane was represented as layers of distinct dielectric constants cor...

journal_title：Journal of molecular biology

authors： Karshikoff A,Spassov V,Cowan SW,Ladenstein R,Schirmer T

更新日期：1994-07-22 00:00:00

abstract：:Nuclear transport factor 2 (NTF2) mediates nuclear import of RanGDP, a central component of many nuclear trafficking pathways. NTF2 is a homodimer and each chain has independent binding sites for RanGDP and nuclear pore proteins (nucleoporins) that contain FxFG sequence repeats. We show here that the monomer-dimer dis...

journal_title：Journal of molecular biology

authors： Chaillan-Huntington C,Butler PJ,Huntington JA,Akin D,Feldherr C,Stewart M

更新日期：2001-11-30 00:00:00

abstract：:Dimerization is indispensible for release of the human immunodeficiency virus protease (PR) from its precursor (Gag-Pol) and ensuing mature-like catalytic activity that is crucial for virus maturation. We show that a single-chain Fv fragment (scFv) of a previously reported monoclonal antibody (mAb1696), which recogniz...

journal_title：Journal of molecular biology

authors： Sayer JM,Aniana A,Louis JM

更新日期：2012-09-14 00:00:00

abstract：:Major histocompatibility complex (MHC) class I proteins are expressed on the cell surface where they present foreign and self-peptides to effector cells of the immune system. While an understanding of the structural prerequisites for antigen presentation has already been achieved, insight into subtype- or peptide-depe...

journal_title：Journal of molecular biology

authors： Narzi D,Becker CM,Fiorillo MT,Uchanska-Ziegler B,Ziegler A,Böckmann RA

更新日期：2012-01-13 00:00:00

abstract：:RNA sites that contain unpaired or mismatched nucleotides can be interaction sites for other macromolecules. C1054, a virtually universally conserved nucleotide in the 16 S (small subunit) ribosomal RNA of Escherichia coli, is part of a highly conserved bulge in helix 34, which has been located at the decoding site of...

journal_title：Journal of molecular biology

authors： Pagel FT,Zhao SQ,Hijazi KA,Murgola EJ

更新日期：1997-04-18 00:00:00

abstract：:By electron microscopy, we have observed immunocomplexes with both negative stain and in amorphous ice using monoclonal antibodies directed against one of the 24 subunits of scorpion haemocyanin. A copy of this subunit occurs at each of the corners of the square-shaped haemocyanin molecule. Three distinct orientations...

journal_title：Journal of molecular biology

authors： Wade RH,Taveau JC,Lamy JN

更新日期：1989-03-20 00:00:00

abstract：:Transverse sections (100 to 140 nm thick) of the flight muscles of the fleshfly Phormia terrae-novae and the housefly Musca domestica were studied. The images of 56 tubular myosin filaments of the fleshfly and 62 filaments of the housefly were digitized and computer processed by rotational averaging. The rotational po...

journal_title：Journal of molecular biology

authors： Beinbrech G,Ashton FT,Pepe FA

更新日期：1988-06-05 00:00:00

abstract：:Through the high-resolution structure of the gramicidin A channel in lamellar phase lipids and the characterization of specific ion peptide interactions, fundamental principles for ion channel selectivity and conductance efficiency are illustrated with atomic resolution detail. Delocalized cation binding in the first ...

journal_title：Journal of molecular biology

authors： Tian F,Cross TA

更新日期：1999-02-05 00:00:00

abstract：:The FLP recombinase interacts with its target sequence with the formation of three distinct DNA-protein complexes. The first complex leaves neither a DNase footprint nor is the DNA protected from methylation by dimethyl sulfate. We have found, however, that the FLP protein is bound predominantly to only one of the thr...

journal_title：Journal of molecular biology

authors： Beatty LG,Sadowski PD

更新日期：1988-11-20 00:00:00

abstract：:Recent experimental results suggest that the native fold, or topology, plays a primary role in determining the structure of the transition state ensemble, at least for small, fast-folding proteins. To investigate the extent of the topological control of the folding process, we studied the folding of simplified models ...

journal_title：Journal of molecular biology

authors： Clementi C,Nymeyer H,Onuchic JN

更新日期：2000-05-19 00:00:00

abstract：:RIG-I-like receptors (RLRs) are nucleic acid sensors that activate antiviral innate immune response. These molecules recognize diverse non-self RNA substrates and are antagonized by several viral inhibitors. We performed an evolutionary analysis of RLR genes (RIG-I, MDA5, and LGP2) in mammals. Results indicated that p...

journal_title：Journal of molecular biology

authors： Cagliani R,Forni D,Tresoldi C,Pozzoli U,Filippi G,Rainone V,De Gioia L,Clerici M,Sironi M

更新日期：2014-03-20 00:00:00

abstract：:The structure of replicating simian virus 40 (SV40) minichromosomes was studied by DNA crosslinking with trimethyl-psoralen. The procedure was used both in vitro with extracted SV40 minichromosomes as well as in vivo with SV40-infected cells. Both procedures gave essentially the same results. Mature SV40 minichromosom...

journal_title：Journal of molecular biology

authors： Sogo JM,Stahl H,Koller T,Knippers R

更新日期：1986-05-05 00:00:00

abstract：:Cdc25B is a dual specificity phosphatase involved in the control of cyclin-dependent kinases and the progression of cells through the cell cycle. A series of minimal domain Cdc25B constructs maintaining catalytic activity have been expressed. The structure of a minimum domain construct binding sulfate was determined a...

journal_title：Journal of molecular biology

authors： Reynolds RA,Yem AW,Wolfe CL,Deibel MR Jr,Chidester CG,Watenpaugh KD

更新日期：1999-10-29 00:00:00

abstract：:We have studied the reversible dissociation of core size DNA from chicken erythrocyte nucleosome core particles in solutions containing 0 X 1 M to 0 X 6 M-NaCl. Dissociation increases with increasing NaCl concentration, increasing temperature and decreasing particle concentration. At high particle concentrations, no f...

journal_title：Journal of molecular biology

authors： Ausio J,Seger D,Eisenberg H

更新日期：1984-06-15 00:00:00

abstract：:Along with the discovery of tumor-driven inflammatory pathways, there has been a considerable progress over the past 10years in understanding the mechanisms leading to cancer immunosurveillance and immunoediting. Several regulatory pathways, typically involved in immune cell homeostasis, are co-opted by cancer cells t...

journal_title：Journal of molecular biology

authors： Rabinovich GA,Conejo-García JR

更新日期：2016-08-14 00:00:00

abstract：:The pH dependence of the amide/solvent hydrogen exchange of individual amide groups in hen egg-white lysozyme has been studied by nuclear magnetic resonance spectroscopy. Lysozyme has been used here as a model for a globular protein to re-examine the hypothesis for the amide/solvent hydrogen exchange reaction proposed...

journal_title：Journal of molecular biology

authors： Pedersen TG,Thomsen NK,Andersen KV,Madsen JC,Poulsen FM

更新日期：1993-03-20 00:00:00

abstract：:We report a new vector, pEVOL, for the incorporation of unnatural amino acids into proteins in Escherichia coli using evolved Methanocaldococcus jannaschii aminoacyl-tRNA synthetase(s) (aaRS)/suppressor tRNA pairs. This new system affords higher yields of mutant proteins through the use of both constitutive and induci...

journal_title：Journal of molecular biology

authors： Young TS,Ahmad I,Yin JA,Schultz PG

更新日期：2010-01-15 00:00:00

abstract：:One aim of computational protein design is to introduce novel enzyme activity into proteins of known structure by predicting mutations that stabilize transition states. Previously, we showed that it is possible to introduce triose phosphate isomerase activity into the ribose-binding protein of Escherichia coli by cons...

journal_title：Journal of molecular biology

authors： Allert M,Dwyer MA,Hellinga HW

更新日期：2007-02-23 00:00:00