Cloning, localization and expression analysis of vacuolar sugar transporters in the CAM plant Ananas comosus (pineapple).

Abstract:

:In photosynthetic tissues of the CAM plant pineapple (Ananas comosus), storage of soluble sugars in the central vacuole during the daytime and their remobilization at night is required to provide carbon skeletons for nocturnal CO(2) fixation. However, soluble sugars produced photosynthetically must also be exported to support growth processes in heterotrophic tissues. To begin to address how vacuolar sugar storage and assimilate partitioning are regulated in A. comosus, degenerate PCR and cDNA library screening were used to clone three candidate sugar transporters from the leaves of this species. Subcellular localization of the three transporters was investigated via expression of YFP-fusion proteins in tobacco epidermal cells and their co-localization with subcellular markers by confocal microscopy. Using this strategy, a putative hexose transporter (AcMST1) and a putative inositol transporter (AcINT1) were identified that both localized to the tonoplast, whereas a putative sucrose transporter (AcSUT1) was found to localize to prevacuolar compartments. A cDNA (AcMST2) with high similarity to a recently characterized tonoplast hexose transporter in Arabidopsis was also identified from an A. comosus fruit EST database. Analyses of transcript abundance indicated that AcMST1 was more highly expressed in fruits compared to leaves of A. comosus, whilst transcripts of AcINT1, AcSUT1, and AcMST2 were more abundant in leaves. Transcript abundance of AcINT1, the putative inositol transporter, showed day-night changes comparable to those of other CAM-related transcripts described in Mesembryanthemum crystallinum. The results are discussed in terms of the role of vacuolar sugar transporters in regulating carbon flow during the diel cycle in CAM plants.

journal_name

J Exp Bot

authors

Antony E,Taybi T,Courbot M,Mugford ST,Smith JA,Borland AM

doi

10.1093/jxb/ern077

subject

Has Abstract

pub_date

2008-01-01 00:00:00

pages

1895-908

issue

7

eissn

0022-0957

issn

1460-2431

pii

ern077

journal_volume

59

pub_type

杂志文章
  • Thrips advisor: exploiting thrips-induced defences to combat pests on crops.

    abstract::Plants have developed diverse defence mechanisms to ward off herbivorous pests. However, agriculture still faces estimated crop yield losses ranging from 25% to 40% annually. These losses arise not only because of direct feeding damage, but also because many pests serve as vectors of plant viruses. Herbivorous thrips ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/ery060

    authors: Steenbergen M,Abd-El-Haliem A,Bleeker P,Dicke M,Escobar-Bravo R,Cheng G,Haring MA,Kant MR,Kappers I,Klinkhamer PGL,Leiss KA,Legarrea S,Macel M,Mouden S,Pieterse CMJ,Sarde SJ,Schuurink RC,De Vos M,Van Wees SCM,Broekg

    更新日期:2018-04-09 00:00:00

  • Pressure gradients along whole culms and leaf sheaths, and other aspects of humidity-induced gas transport in Phragmites australis.

    abstract::Emergent aquatic macrophytes growing in waterlogged anaerobic sediments overlain by deep water require particularly efficient ventilating systems. In Phragmites australis (Cav.) Trin. ex Steud, pressurized gas flows, generated by humidity-induced diffusion of air into leaf sheaths, enhance oxygen transport to below-gr...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erm017

    authors: Afreen F,Zobayed SM,Armstrong J,Armstrong W

    更新日期:2007-01-01 00:00:00

  • Naphthylphthalamic acid and the mechanism of polar auxin transport.

    abstract::Our current understanding of how plants move auxin through their tissues is largely built on the use of polar auxin transporter inhibitors. Although the most important proteins that mediate auxin transport and its regulation have probably all been identified and the mapping of their interactions is well underway, mech...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/erx323

    authors: Teale W,Palme K

    更新日期:2018-01-04 00:00:00

  • A copia-like retrotransposon insertion in the upstream region of the SHATTERPROOF1 gene, BnSHP1.A9, is associated with quantitative variation in pod shattering resistance in oilseed rape.

    abstract::Seed loss resulting from pod shattering is a major constraint in production of oilseed rape (Brassica napus L.). However, the molecular mechanisms underlying pod shatter resistance are not well understood. Here, we show that the pod shatter resistance at quantitative trait locus qSRI.A9.1 is controlled by one of the B...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eraa281

    authors: Liu J,Zhou R,Wang W,Wang H,Qiu Y,Raman R,Mei D,Raman H,Hu Q

    更新日期:2020-09-19 00:00:00

  • Homology modeling and in vivo functional characterization of the zinc permeation pathway in a heavy metal P-type ATPase.

    abstract::The P1B ATPase heavy metal ATPase 4 (HMA4) is responsible for zinc and cadmium translocation from roots to shoots in Arabidopsis thaliana. It couples ATP hydrolysis to cytosolic domain movements, enabling metal transport across the membrane. The detailed mechanism of metal permeation by HMA4 through the membrane remai...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/ery353

    authors: Lekeux G,Crowet JM,Nouet C,Joris M,Jadoul A,Bosman B,Carnol M,Motte P,Lins L,Galleni M,Hanikenne M

    更新日期:2019-01-01 00:00:00

  • Comparative transcriptome analysis in Arabidopsis ein2/ore3 and ahk3/ore12 mutants during dark-induced leaf senescence.

    abstract::Leaf senescence involves degenerative but active biological processes that require balanced regulation of pro- and anti-senescing activities. Ethylene and cytokinin are major antagonistic regulatory hormones that control the timing and progression rate of leaf senescence. To identify the roles of these hormones in the...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/ery137

    authors: Kim J,Park SJ,Lee IH,Chu H,Penfold CA,Kim JH,Buchanan-Wollaston V,Nam HG,Woo HR,Lim PO

    更新日期:2018-05-25 00:00:00

  • The Medicago truncatula Yellow Stripe1-Like3 gene is involved in vascular delivery of transition metals to root nodules.

    abstract::Symbiotic nitrogen fixation carried out in legume root nodules requires transition metals. These nutrients are delivered by the host plant to the endosymbiotic nitrogen-fixing bacteria living within the nodule cells, a process in which vascular transport is essential. As members of the Yellow Stripe-Like (YSL) family ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eraa390

    authors: Castro-Rodríguez R,Abreu I,Reguera M,Novoa-Aponte L,Mijovilovich A,Escudero V,Jiménez-Pastor FJ,Abadía J,Wen J,Mysore KS,Álvarez-Fernández A,Küpper H,Imperial J,González-Guerrero M

    更新日期:2020-12-31 00:00:00

  • Iron deficiency differently affects peroxidase isoforms in sunflower.

    abstract::The response of both specific (ascorbate peroxidase, APX) and unspecific (POD) peroxidases and H(2)O(2) content of sunflower plants (Helianthus annuus L. cv. Hor) grown hydroponically with (C) or without (-Fe) iron in the nutrient solution were analysed to verify whether iron deficiency led to cell oxidative status. I...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:

    authors: Ranieri A,Castagna A,Baldan B,Soldatini GF

    更新日期:2001-01-01 00:00:00

  • The multi-protein family of Arabidopsis sulphotransferases and their relatives in other plant species.

    abstract::All members of the sulphotransferase (SOT, EC 2.8.2.-) protein family use 3'-phosphoadenosine 5'-phosphosulphate (PAPS) as the sulphuryl donor and transfer the sulphonate group to an appropriate hydroxyl group of several classes of substrates. These enzymes have highly conserved domains and can be found in eubacteria ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/erh183

    authors: Klein M,Papenbrock J

    更新日期:2004-08-01 00:00:00

  • Root-ABA1 QTL affects root lodging, grain yield, and other agronomic traits in maize grown under well-watered and water-stressed conditions.

    abstract::A major QTL affecting root traits and leaf ABA concentration was identified in maize (Zea mays L.) and named root-ABA1. For this QTL, back-cross-derived lines (BDLs) homozygous either for the (+) or for the (-) allele increasing or decreasing, respectively, root size and leaf ABA concentration, were developed. This st...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erl161

    authors: Landi P,Sanguineti MC,Liu C,Li Y,Wang TY,Giuliani S,Bellotti M,Salvi S,Tuberosa R

    更新日期:2007-01-01 00:00:00

  • Alteration of flavonoid pigmentation patterns during domestication of food crops.

    abstract::Flavonoids are plant pigments that provide health benefits for human and animal consumers. Understanding why domesticated crops have altered pigmentation patterns and unraveling the molecular/genetic mechanisms that underlie this will facilitate the breeding of new (healthier) varieties. We present an overview of chan...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erz141

    authors: Paauw M,Koes R,Quattrocchio FM

    更新日期:2019-08-07 00:00:00

  • Aethionema arabicum: a novel model plant to study the light control of seed germination.

    abstract::The timing of seed germination is crucial for seed plants and is coordinated by internal and external cues, reflecting adaptations to different habitats. Physiological and molecular studies with lettuce and Arabidopsis thaliana have documented a strict requirement for light to initiate germination and identified many ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erz146

    authors: Mérai Z,Graeber K,Wilhelmsson P,Ullrich KK,Arshad W,Grosche C,Tarkowská D,Turečková V,Strnad M,Rensing SA,Leubner-Metzger G,Mittelsten Scheid O

    更新日期:2019-06-28 00:00:00

  • Organization of cell walls in Sandersonia aurantiaca floral tissue.

    abstract::Visual symptoms of the onset of senescence in Sandersonia aurantiaca flowers begin with fading of flower colour and wilting of the tissue. When fully senescent, the flowers form a papery shell that remains attached to the plant. The cell walls of these flowers have been examined to determine whether there are wall mod...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jexbot/53.368.513

    authors: O'Donoghue EM,Somerfield SD,Heyes JA

    更新日期:2002-03-01 00:00:00

  • Changes in the chloroplast proteome following water deficit and subsequent watering in a high- and a low-drought-tolerant genotype of Festuca arundinacea.

    abstract::Festuca arundinacea is one of the most drought-tolerant species within the Lolium-Festuca complex and was used as a model for research aimed at identifying the chloroplast components involved in the proteomic response for drought stress in forage grasses. Individual F. arundinacea genotypes with contrasting levels of ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/ers265

    authors: Kosmala A,Perlikowski D,Pawłowicz I,Rapacz M

    更新日期:2012-10-01 00:00:00

  • The kiwifruit lycopene beta-cyclase plays a significant role in carotenoid accumulation in fruit.

    abstract::The composition of carotenoids, along with anthocyanins and chlorophyll, accounts for the distinctive range of colour found in the Actinidia (kiwifruit) species. Lutein and beta-carotene are the most abundant carotenoids found during fruit development, with beta-carotene concentration increasing rapidly during fruit m...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erp218

    authors: Ampomah-Dwamena C,McGhie T,Wibisono R,Montefiori M,Hellens RP,Allan AC

    更新日期:2009-01-01 00:00:00

  • Sulfur: the heart of nitric oxide-dependent redox signalling.

    abstract::Nitric oxide (NO), more benign than its more reactive and damaging related molecules, reactive oxygen species (ROS), is perfectly suited for duties as a redox signalling molecule. A key route for NO bioactivity is through S-nitrosation, the addition of an NO moiety to a protein Cys thiol (-SH). This redox-based, post-...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/erz135

    authors: Umbreen S,Lubega J,Loake GJ

    更新日期:2019-08-19 00:00:00

  • Intron-regulated expression of SUVH3, an Arabidopsis Su(var)3-9 homologue.

    abstract::SU(VAR)3-9 proteins are key regulators of heterochromatin structure and function in plants, mammals, Drosophila, and yeast. In contrast to animals and fungi, plants contain numerous Su(var)3-9 homologues (SUVH), the members of which form a discrete subfamily. The SU(VAR)3-9 and SUVH proteins associate with heterochrom...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erl093

    authors: Casas-Mollano JA,Lao NT,Kavanagh TA

    更新日期:2006-01-01 00:00:00

  • A highly charged region in the middle domain of plant endoplasmic reticulum (ER)-localized heat-shock protein 90 is required for resistance to tunicamycin or high calcium-induced ER stresses.

    abstract::Heat-shock protein 90 (HSP90) is a highly conserved molecular chaperone that is involved in modulating a multitude of cellular processes under both physiological and stress conditions. In Arabidopsis, there are seven HSP90 isoforms (HSP90.1-HSP90.7) that are localized in the cytoplasm/nucleus, mitochondrion, chloropla...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eru403

    authors: Chong LP,Wang Y,Gad N,Anderson N,Shah B,Zhao R

    更新日期:2015-01-01 00:00:00

  • Phloem sap proteins: their identities and potential roles in the interaction between plants and phloem-feeding insects.

    abstract::The phloem is a well-known target of sucking and piercing insects that utilize the transported fluid as their major nutrient source. In addition to small molecules like sugars and amino acids, phloem sap of higher land plants contains proteins that can accumulate up to high concentrations. Although the knowledge about...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/erj087

    authors: Kehr J

    更新日期:2006-01-01 00:00:00

  • Ca2+-dependent nuclease is involved in DNA degradation during the formation of the secretory cavity by programmed cell death in fruit of Citrus grandis 'Tomentosa'.

    abstract::The secretory cavity is a typical structure in Citrus fruit and is formed by schizolysigeny. Previous reports have indicated that programmed cell death (PCD) is involved in the degradation of secretory cavity cells in the fruit, and that the spatio-temporal location of calcium is closely related to nuclear DNA degrada...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eraa199

    authors: Bai M,Liang M,Huai B,Gao H,Tong P,Shen R,He H,Wu H

    更新日期:2020-08-06 00:00:00

  • HYL1 is required for establishment of stamen architecture with four microsporangia in Arabidopsis.

    abstract::The stamen produces pollen grains for pollination in higher plants. Coordinated development of four microsporangia in the stamen is essential for normal fertility. The roles of miR165/166-directed pathways in the establishment of adaxial-abaxial polarity have been well defined in leaves. However, the molecular mechani...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/ert178

    authors: Lian H,Li X,Liu Z,He Y

    更新日期:2013-08-01 00:00:00

  • Sulfate transport systems in plants: functional diversity and molecular mechanisms underlying regulatory coordination.

    abstract::Sulfate transporters are integral membrane proteins controlling the flux of sulfate (SO42-) entering the cells and subcellular compartments across the membrane lipid bilayers. Sulfate uptake is a dynamic biological process that occurs in multiple cell layers and organs in plants. In vascular plants, sulfate ions are t...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审

    doi:10.1093/jxb/erz132

    authors: Takahashi H

    更新日期:2019-08-19 00:00:00

  • Variation between rice accessions in photosynthetic induction in flag leaves and underlying mechanisms.

    abstract::Several breeding initiatives have sought to improve flag leaf performance as its health and physiology are closely correlated to rice yield. Previous studies have described natural variation of photosynthesis for flag leaves; however, none have examined their performance under the non-steady-state conditions that prev...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/eraa520

    authors: Acevedo-Siaca L,Coe R,Quick WP,Long SP

    更新日期:2020-11-07 00:00:00

  • Potential contribution of strigolactones in regulating scion growth and branching in grafted grapevine in response to nitrogen availability.

    abstract::In grafted plants, rootstocks assure the mineral nutrition of the scion and modify its development. In this study, we show that two grapevine rootstock genotypes have different shoot branching architectures when cultivated as cuttings and that this trait is transmitted to the scion when grafted. Shoot branching plasti...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/ery206

    authors: Cochetel N,Météier E,Merlin I,Hévin C,Pouvreau JB,Coutos-Thévenot P,Hernould M,Vivin P,Cookson SJ,Ollat N,Lauvergeat V

    更新日期:2018-07-18 00:00:00

  • StMYB44 negatively regulates anthocyanin biosynthesis at high temperatures in tuber flesh of potato.

    abstract::High temperatures are known to reduce anthocyanin accumulation in a number of diverse plant species. In potato (Solanum tuberosum L.), high temperature significantly reduces tuber anthocyanin pigment content. However, the mechanism of anthocyanin biosynthesis in potato tuber under heat stress remains unknown. Here we ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erz194

    authors: Liu Y,Lin-Wang K,Espley RV,Wang L,Li Y,Liu Z,Zhou P,Zeng L,Zhang X,Zhang J,Allan AC

    更新日期:2019-08-07 00:00:00

  • Alleviation of rapid, futile ammonium cycling at the plasma membrane by potassium reveals K+-sensitive and -insensitive components of NH4+ transport.

    abstract::Futile plasma membrane cycling of ammonium (NH4+) is characteristic of low-affinity NH4+ transport, and has been proposed to be a critical factor in NH4+ toxicity. Using unidirectional flux analysis with the positron-emitting tracer 13N in intact seedlings of barley (Hordeum vulgare L.), it is shown that rapid, futile...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erm309

    authors: Szczerba MW,Britto DT,Balkos KD,Kronzucker HJ

    更新日期:2008-01-01 00:00:00

  • The alpha-subunit of the heterotrimeric G-protein affects jasmonate responses in Arabidopsis thaliana.

    abstract::Heterotrimeric G-proteins have been implicated in having a role in many plant signalling pathways. To understand further the role of G-proteins, a preliminary experiment was performed to assess the impact of the G alpha subunit loss-of-function mutation gpa1-1 on the Arabidopsis transcriptome. The analysis indicated t...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erp060

    authors: Okamoto H,Göbel C,Capper RG,Saunders N,Feussner I,Knight MR

    更新日期:2009-01-01 00:00:00

  • A role for jasmonates in the release of dormancy by cold stratification in wheat.

    abstract::Hydration at low temperatures, commonly referred to as cold stratification, is widely used for releasing dormancy and triggering germination in a wide range of species including wheat. However, the molecular mechanism that underlies its effect on germination has largely remained unknown. Our previous studies showed th...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erw172

    authors: Xu Q,Truong TT,Barrero JM,Jacobsen JV,Hocart CH,Gubler F

    更新日期:2016-05-01 00:00:00

  • PacCYP707A2 negatively regulates cherry fruit ripening while PacCYP707A1 mediates drought tolerance.

    abstract::Sweet cherry is a non-climacteric fruit and its ripening is regulated by abscisic acid (ABA) during fruit development. In this study, four cDNAs (PacCYP707A1-4) encoding 8'-hydroxylase, a key enzyme in the oxidative catabolism of ABA, were identified in sweet cherry fruits using tobacco rattle virus-induced gene silen...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/erv169

    authors: Li Q,Chen P,Dai S,Sun Y,Yuan B,Kai W,Pei Y,He S,Liang B,Zhang Y,Leng P

    更新日期:2015-07-01 00:00:00

  • Role of HSP101 in the stimulation of nodal root development from the coleoptilar node by light and temperature in maize (Zea mays L.) seedlings.

    abstract::Nodal roots (NRs) constitute the prevalent root system of adult maize plants. NRs emerge from stem nodes located below or above ground, and little is known about their inducing factors. Here, it is shown that precocious development of NRs at the coleoptilar node (NRCNs) occurred in maize seedlings when: (i) dark grown...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章

    doi:10.1093/jxb/err186

    authors: López-Frías G,Martínez LM,Ponce G,Cassab GI,Nieto-Sotelo J

    更新日期:2011-08-01 00:00:00