Mutations in components of the Wnt signaling pathway in gastric cancer.

Abstract:

AIM:To explore the contribution of AXIN1, AXIN2 and beta-catenin, components of Wnt signaling pathway, to the carcinogenesis of gastric cancer (GC), we examined AXIN1, AXIN2 exon7 and CTNNB1 (encoding beta-catenin) exon3 mutations in 70 GCs. METHODS:The presence of mutations was identified by polymerase chain reaction (PCR)-based denaturing high-performance liquid chromatography and direct DNA sequencing. Beta-catenin expression was detected by immunohistochemical analysis. RESULTS:Among the 70 GCs, 5 (7.1%) had mutations in one or two of these three components. A frameshift mutation (1 bp deletion) in exon7 of AXIN2 was found in one case. Four cases, including the case with a mutation in AXIN2, had frameshift mutations and missense mutations in AXIN1. Five single nucleotide polymorphisms (SNPs), 334 C>T, 874 C>T, 1396 G>A, 1690 C>T and 1942 T>G, were identified in AXIN1. A frameshift mutation (27 bp deletion) spanning exon3 of CTNNB1 was observed in one case. All four cases with mutations in AXIN1 and AXIN2 showed nuclear beta-catenin expression. CONCLUSION:These data indicate that the mutations in AXIN1 and AXIN2 may contribute to gastric carcino-genesis.

journal_name

World J Gastroenterol

authors

Pan KF,Liu WG,Zhang L,You WC,Lu YY

doi

10.3748/wjg.14.1570

subject

Has Abstract

pub_date

2008-03-14 00:00:00

pages

1570-4

issue

10

eissn

1007-9327

issn

2219-2840

journal_volume

14

pub_type

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