当前位置： SCI文献检索 > JOURNAL OF NEUROCHEMISTRY期刊下所有文献 > Expression of multiple AQP4 pools in the plasma membrane and their association with the dystrophin complex.

Expression of multiple AQP4 pools in the plasma membrane and their association with the dystrophin complex.

Abstract：

:Altered aquaporin-4 (AQP4) expression has been reported in brain edema, tumors, muscular dystrophy, and neuromyelitis optica. However, the plasma membrane organization of AQP4 and its interaction with proteins such as the dystrophin-associated protein complex are not well understood. In this study, we used sucrose density gradient ultracentrifugation and 2D blue native/sodium dodecyl sulfate-polyacrylamide gel electrophoresis and showed the expression of several AQP4 multi-subunit complexes (pools) of different sizes, ranging from > 1 MDa to approximately 500 kDa and containing different ratios of the 30/32 kDa AQP4 isoforms, indicative of orthogonal arrays of particles of various sizes. A high molecular weight pool co-purified with dystrophin and beta-dystroglycan and was drastically reduced in the skeletal muscle of mdx3cv mice, which have no dystrophin. The number and size of the AQP4 pools were the same in the kidney where dystrophin is not expressed, suggesting the presence of dystrophin-like proteins for their expression. We found that AQP2 is expressed only in one major pool of approximately 500 kDa, indicating that the presence of different pools is a peculiarity of AQP4 rather than a widespread feature in the AQP family. Finally, in skeletal muscle caveolin-3 did not co-purify with any AQP4 pool, indicating the absence of interaction of the two proteins and confirming that caveolae and orthogonal arrays of particles are two independent plasma membrane microdomains. These results contribute to a better understanding of AQP4 membrane organization and raise the possibility that abnormal expression of specific AQP4 pools may be found in pathological states.

journal_name

J Neurochem

journal_title

Journal of neurochemistry

authors

Nicchia GP,Cogotzi L,Rossi A,Basco D,Brancaccio A,Svelto M,Frigeri A

doi

10.1111/j.1471-4159.2008.05302.x

subject

Has Abstract

pub_date

2008-06-01 00:00:00

pages

2156-65

issue

6

eissn

0022-3042

issn

1471-4159

pii

JNC5302

journal_volume

105

pub_type

杂志文章

相关文献

JOURNAL OF NEUROCHEMISTRY文献大全
  • Sex differences in neonatal mouse brain injury after hypoxia-ischemia and adaptaquin treatment.

    abstract：:Hypoxia-inducible factor prolyl 4-hydroxylases (HIF-PHDs) are important targets against oxidative stress. We hypothesized that inhibition HIF-PHD by adaptaquin reduces hypoxic-ischemic brain injury in a neonatal mouse model. The pups were treated intraperitoneally immediately with adaptaquin after hypoxia-ischemia (HI...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/jnc.14790

    authors： Li K,Li T,Wang Y,Xu Y,Zhang S,Culmsee C,Wang X,Zhu C

    更新日期：2019-09-01 00:00:00

  • Glutamate release from activated microglia requires the oxidative burst and lipid peroxidation.

    abstract：:When activated by proinflammatory stimuli, microglia release substantial levels of glutamate, and mounting evidence suggests this contributes to neuronal damage during neuroinflammation. Prior studies indicated a role for the Xc exchange system, an amino acid transporter that antiports glutamate for cystine. Because c...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.2007.04487.x

    authors： Barger SW,Goodwin ME,Porter MM,Beggs ML

    更新日期：2007-06-01 00:00:00

  • PAL31 may play an important role as inflammatory modulator in the repair process of the spinal cord injury rat.

    abstract：:Functional regeneration in a complete T8 transection model Cheng et al. (1996) and most recently, acidic fibroblast growth factor (aFGF; also known as FGF-1) involved in the repair process of the spinal cord injury (SCI) rat Tsai et al. (2008) have been reported. To further reveal the mechanism of the repair process o...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.2008.05865.x

    authors： Shen LF,Cheng H,Tsai MC,Kuo HS,Chak KF

    更新日期：2009-03-01 00:00:00

  • Coupling of serotonin 5-HT1B receptors to activation of mitogen-activated protein kinase (ERK-2) and p70 S6 kinase signaling systems.

    abstract：:Little is known about the coupling of serotonin 5-HT1B receptors to cellular signals other than cyclic AMP. In the present studies, the activation by 5-HT1B receptors of p70 S6 kinase and the mitogen-activated protein kinase (MAP kinase) ERK-2 was investigated. Studies were performed by using both nontransfected Chine...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1046/j.1471-4159.1998.71031059.x

    authors： Pullarkat SR,Mysels DJ,Tan M,Cowen DS

    更新日期：1998-09-01 00:00:00

  • Cytochrome c release precedes mitochondrial membrane potential loss in cerebellar granule neuron apoptosis: lack of mitochondrial swelling.

    abstract：:It has been suggested that release of cytochrome c (Cyt c) from mitochondria during apoptotic death is through opening of the mitochondrial permeability transition pore followed by swelling-induced rupture of the mitochondrial outer membrane. However, this remains controversial and may vary with cell type and model sy...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1046/j.1471-4159.2002.01049.x

    authors： Wigdal SS,Kirkland RA,Franklin JL,Haak-Frendscho M

    更新日期：2002-09-01 00:00:00

  • Choline acetyltransferase activity and total protein content in selected optic areas of the rat after complete light-deprivation during CNS development.

    abstract：:Choline acetyltransferase (acetyl-CoA: choline O-acetyltransferase, I.U.B. 2.3.1.6) activity and total protein content in visual and extra-visual areas were compared in normal Long-Evans rats and in rats subjected to complete light-deprivation for 21 days from birth. The enzyme activity and the protein content in the ...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.1968.tb10323.x

    authors： Maletta GJ,Timiras PS

    更新日期：1968-08-01 00:00:00

  • Frame-shifted amyloid precursor protein found in Alzheimer's disease and Down's syndrome increases levels of secreted amyloid beta40.

    abstract：:Frame-shifted amyloid precursor protein (APP(+1)), which has a truncated out-of-frame C-terminus, accumulates in the neuropathological hallmarks of patients with Alzheimer's disease pathology. To study a possible involvement of APP(+1) in the pathogenesis of Alzheimer's disease, we expressed APP695 and APP(+1) in the ...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.2004.02528.x

    authors： van Dijk R,Fischer DF,Sluijs JA,Sonnemans MA,Hobo B,Mercken L,Mann DM,Hol EM,van Leeuwen FW

    更新日期：2004-08-01 00:00:00

  • Metallothionein induction in neonatal rat primary astrocyte cultures protects against methylmercury cytotoxicity.

    abstract：:Metallothionein (MT) protein and mRNA levels were monitored following exposure of rat neonatal primary astrocyte cultures to methylmercury (MeHg). MT-I and MT-II mRNAs were probed on northern blots with an [alpha-32P]dCTP-labeled synthetic cDNA probe specific for rat MT mRNA. MT-I and MT-II mRNAs were detected in untr...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1046/j.1471-4159.1995.65041562.x

    authors： Rising L,Vitarella D,Kimelberg HK,Aschner M

    更新日期：1995-10-01 00:00:00

  • Chronic dizocilpine (MK-801) reversibly delays GABA(A) receptor maturation in cerebellar granule neurons in vitro.

    abstract：:We investigated the effect of chronically blocking NMDA receptor stimulation to examine changes in GABA(A) receptor expression and pharmacology in cerebellar granule cells at different stages of maturation. We have previously shown that NMDA-selective glutamate receptor stimulation alters GABA(A) receptor pharmacology...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1046/j.1471-4159.1998.71020693.x

    authors： Wang XH,Zhu WJ,Corsi L,Ikonomovic S,Paljug WR,Vicini S,Grayson DR

    更新日期：1998-08-01 00:00:00

  • Biochemical and immunochemical studies on the GABAergic system in the rat fallopian tube and ovary.

    abstract：:gamma-Aminobutyric acid (GABA) and glutamic acid decarboxylase (GAD) activities were measured in the ovary and the Fallopian tube of rats and compared with brain values. GABA levels in the Fallopian tube were about twice as high as in the brain, while in the ovary they represented only about 5% of the amino acid conte...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.1984.tb06686.x

    authors： Apud JA,Tappaz ML,Celotti F,Negri-Cesi P,Masotto C,Racagni G

    更新日期：1984-07-01 00:00:00

  • The alpha 1 and alpha 6 subunits can coexist in the same cerebellar GABAA receptor maintaining their individual benzodiazepine-binding specificities.

    abstract：:Two GABAA receptor subunit-specific antibodies anti-alpha 6 and anti-alpha 1 have been used for elucidating the relationship between the presence of alpha 1 and/or alpha 6 subunits in the cerebellar GABAA receptors and the benzodiazepine-binding specificity. Receptor immunoprecipitation with the subunit-specific antib...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1046/j.1471-4159.1996.66020685.x

    authors： Khan ZU,Gutiérrez A,De Blas AL

    更新日期：1996-02-01 00:00:00

  • Essential role for integrin linked kinase in Akt-mediated integrin survival signaling in hippocampal neurons.

    abstract：:Activation of integrin receptors in neurons can promote cell survival and synaptic plasticity, but the underlying signal transduction pathway(s) is unknown. We report that integrin signaling prevents apoptosis of embryonic hippocampal neurons by a mechanism involving integrin-linked kinase (ILK) that activates Akt kin...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1046/j.1471-4159.2003.01579.x

    authors： Gary DS,Milhavet O,Camandola S,Mattson MP

    更新日期：2003-02-01 00:00:00

  • [3H]nitrobenzylthioinosine binding as a probe for the study of adenosine uptake sites in brain.

    abstract：:The binding of the potent adenosine uptake inhibitor [3H]nitrobenzylthioinosine ([3H]NBI) to brain membrane fractions was investigated. Reversible, saturable, specific, high-affinity binding was demonstrated in both rat and human brain. The KD in both was 0.15 nM with Bmax values of 140-200 fmol/mg protein. Linear Sca...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.1982.tb04717.x

    authors： Marangos PJ,Patel J,Clark-Rosenberg R,Martino AM

    更新日期：1982-07-01 00:00:00

  • Pre- and postnatal developmental changes of adrenoceptor subtypes in rat brain.

    abstract：:beta-Adrenergic receptor subtypes, beta 1 and beta 2, were studied during pre- and postnatal development in the rat brain. [125I]Iodocyanopindolol (6-300 pmol/L) binding assays in the presence of 5-hydroxytryptamine (0.6-6 mumol/L) were used to measure exclusively beta-adrenergic receptors. In forebrain tissue, satura...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.1991.tb08235.x

    authors： Erdtsieck-Ernste BH,Feenstra MG,Boer GJ

    更新日期：1991-09-01 00:00:00

  • Synaptotagmin I is a molecular target for lead.

    abstract：:Lead poisoning can cause a wide range of symptoms with particularly severe clinical effects on the CNS. Lead can increase spontaneous neurotransmitter release but decrease evoked neurotransmitter release. These effects may be caused by an interaction of lead with specific molecular targets involved in neurotransmitter...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1046/j.1471-4159.2001.00168.x

    authors： Bouton CM,Frelin LP,Forde CE,Arnold Godwin H,Pevsner J

    更新日期：2001-03-01 00:00:00

  • Alternative pathways of glucose utilization in brain; changes in the pattern of glucose utilization in brain resulting from treatment of rats with 6-aminonicotinamide.

    abstract：:The effect of 6-aminonicotinamide (6AN) treatment on the activities of alternative pathways of glucose metabolism in 20-day-old rat brain was evaluated by measurements of yields of 14CO2 from glucose labeled with 14C on carbons 1, 2, 3 + 4, or 6 and uniformly labeled glucose, and from the incorporation of 14C from spe...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.1981.tb06318.x

    authors： Hothersall JS,Zubairu S,McLean P,Greenbaum AL

    更新日期：1981-12-01 00:00:00

  • GM1 ganglioside treatment of PC12 cells stimulates ganglioside, glycolipid, and lipid, but not glycoprotein synthesis independently from the effects of nerve growth factor.

    abstract：:The incorporation of radioactive precursors into gangliosides and other glycolipids, glycoproteins, and total lipids has been studied in rat pheochromocytoma PC12 cells. Starting with the same PC12 cell pool, cultures displaying different degrees of neuritic expression in response to nerve growth factor (NGF) and comb...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.1986.tb13006.x

    authors： Katoh-Semba R,Skaper SD,Varon S

    更新日期：1986-02-01 00:00:00

  • Extracellular glutamate is increased in thalamus during thiamine deficiency-induced lesions and is blocked by MK-801.

    abstract：:The current study measured extracellular fluid (ECF) levels of excitatory amino acids before and during the onset of thiamine deficiency-induced pathologic lesions. Male Sprague-Dawley rats were treated with daily pyrithiamine (0.25 mg/kg i.p.) and a thiamine-deficient diet (PTD). Microdialysates were simultaneously c...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.1993.tb07457.x

    authors： Langlais PJ,Zhang SX

    更新日期：1993-12-01 00:00:00

  • Lactadherin/MFG-E8 is essential for microglia-mediated neuronal loss and phagoptosis induced by amyloid β.

    abstract：:Nanomolar β-amyloid peptide (Aβ) can induce neuronal loss in culture by activating microglia to phagocytose neurons. We report here that this neuronal loss is mediated by the bridging protein lactadherin/milk-fat globule epidermal growth factor-like factor 8 (MFG-E8), which is released by Aβ-activated microglia, binds...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/jnc.12288

    authors： Neniskyte U,Brown GC

    更新日期：2013-08-01 00:00:00

  • Synaptosomal and brain mitochondrial lipids in hibernating and cold-acclimated golden hamsters.

    abstract：:Synaptosomes and mitochondria were isolated from the brains of warm-adapted, hibernating, and cold-acclimated golden hamsters (Mesocricetus auratus). Lipid extracts of these subcellular fractions were prepared and assayed for plasmenylethanolamine (ethanolamine plasmalogen) and cholesterol levels. The ganglioside comp...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.1983.tb08098.x

    authors： Demediuk P,Moscatelli EA

    更新日期：1983-04-01 00:00:00

  • Cyclic AMP-mediated long-term facilitation of glycinergic transmission in developing spinal dorsal horn neurons.

    abstract：:cAMP is known to regulate neurotransmitter release via protein kinase A (PKA)-dependent and/or PKA-independent signal transduction pathways at a variety of central synapses. Here we report the cAMP-mediated long-lasting enhancement of glycinergic transmission in developing rat spinal substantia gelatinosa neurons. For...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.2009.06275.x

    authors： Choi IS,Nakamura M,Cho JH,Park HM,Kim SJ,Kim J,Lee JJ,Choi BJ,Jang IS

    更新日期：2009-09-01 00:00:00

  • Binding of the repressor complex REST-mSIN3b by small molecules restores neuronal gene transcription in Huntington's disease models.

    abstract：:Transcriptional dysregulation is a hallmark of Huntington's disease (HD) and one cause of this dysregulation is enhanced activity of the REST-mSIN3a-mSIN3b-CoREST-HDAC repressor complex, which silences transcription through REST binding to the RE1/NRSE silencer. Normally, huntingtin (HTT) prevents this binding, allowi...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/jnc.12348

    authors： Conforti P,Zuccato C,Gaudenzi G,Ieraci A,Camnasio S,Buckley NJ,Mutti C,Cotelli F,Contini A,Cattaneo E

    更新日期：2013-10-01 00:00:00

  • Pharmacological evidence for the involvement of calcium/calmodulin in serotonin 5-HT3 receptor-mediated cation permeability in NG 108-15 cells.

    abstract：:In NG 108-15 clonal cells, extracellular application of micromolar concentrations of serotonin [5-hydroxytryptamine (5-HT)] and substance P induces the opening of a cation permeability monitored by the influx of [14C]-guanidinium. The serotoninergic component of this cation permeability is linked to 5-HT3 receptor act...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1046/j.1471-4159.1994.62062224.x

    authors： Riad M,Hamon M,Emerit MB

    更新日期：1994-06-01 00:00:00

  • The human N-methyl-D-aspartate receptor 2C subunit: genomic analysis, distribution in human brain, and functional expression.

    abstract：:cDNAs encoding four isoforms of the human NMDA receptor (NMDAR) NMDAR2C (hNR2C-1, -2, -3, and -4) have been isolated and characterized. The overall identity of the deduced amino acid sequences of human and rat NR2C-1 is 89.0%. The sequences of the rat and human carboxyl termini (Gly925-Val1,236) are encoded by differe...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1046/j.1471-4159.1998.71051953.x

    authors： Daggett LP,Johnson EC,Varney MA,Lin FF,Hess SD,Deal CR,Jachec C,Lu CC,Kerner JA,Landwehrmeyer GB,Standaert DG,Young AB,Harpold MM,Veliçelebi G

    更新日期：1998-11-01 00:00:00

  • Pre-synaptic glycine GlyT1 transporter--NMDA receptor interaction: relevance to NMDA autoreceptor activation in the presence of Mg2+ ions.

    abstract：:Rat hippocampal glutamatergic terminals possess NMDA autoreceptors whose activation by low micromolar NMDA elicits glutamate exocytosis in the presence of physiological Mg(2+) (1.2 mM), the release of glutamate being significantly reduced when compared to that in Mg(2+)-free condition. Both glutamate and glycine were ...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.2011.07223.x

    authors： Musante V,Summa M,Cunha RA,Raiteri M,Pittaluga A

    更新日期：2011-05-01 00:00:00

  • Regulation of MARCKS and MARCKS-related protein expression in BV-2 microglial cells in response to lipopolysaccharide.

    abstract：:Myristoylated alanine-rich C kinase substrate (MARCKS) and MARCKS-related protein (MRP) have been implicated in membrane-cytoskeletal events underlying cell adhesion, migration, secretion, and phagocytosis. In BV-2 microglial cells, lipopolysaccharide (LPS) elicited a dose-dependent increase in mRNA of both MRP (sixfo...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1046/j.1471-4159.2001.00458.x

    authors： Sunohara JR,Ridgway ND,Cook HW,Byers DM

    更新日期：2001-08-01 00:00:00

  • Behavioral deficits associated with dietary induction of decreased brain docosahexaenoic acid concentration.

    abstract：:Docosahexaenoic acid (DHA), an n-3 fatty acid, is rapidly deposited during the period of rapid brain development. The influence of n-3 fatty acid deficiency on learning performance in adult rats over two generations was investigated. Rats were fed either an n-3 fatty acid-adequate (n-3 Adq) or -deficient (n-3 Def) die...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1046/j.1471-4159.2000.0752563.x

    authors： Moriguchi T,Greiner RS,Salem N Jr

    更新日期：2000-12-01 00:00:00

  • CSF proteomic analysis reveals persistent iron deficiency-induced alterations in non-human primate infants.

    abstract：:Iron deficiency (ID) anemia during infancy results in long-term neurological consequences, yet the mediating mechanisms remain unclear. Infant monkeys often become naturally anemic during the first 6 months of life, presenting an opportunity to determine the effect of developmental iron deficiency. After weaning, anim...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.2007.05113.x

    authors： Geguchadze RN,Coe CL,Lubach GR,Clardy TW,Beard JL,Connor JR

    更新日期：2008-04-01 00:00:00

  • Phospholipase A2 modulates different subtypes of excitatory amino acid receptors: autoradiographic evidence.

    abstract：:Exogenous phospholipases have been used extensively as tools to study the role of membrane lipids in receptor mechanisms. We used in vitro quantitative autoradiography to evaluate the effect of phospholipase A2 (PLA2) on N-methyl-D-aspartate (NMDA) and non-NMDA glutamate receptors in rat brain. PLA2 pretreatment induc...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.1993.tb05843.x

    authors： Catania MV,Hollingsworth Z,Penney JB,Young AB

    更新日期：1993-01-01 00:00:00

  • Reevaluation of taurine levels and distribution of cysteic acid decarboxylase in developing human fetal brain regions.

    abstract：:The possibilities of interference by glycerophosphoryl ethanolamine (GPE) in the estimation of taurine levels in cerebral cortex, midbrain, cerebellum, medullapons, and spinal cord of developing human fetal brain regions were eliminated by hydrolyzing tissue extracts with 6 M HCl. Cysteic acid thus produced was separa...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.1988.tb10564.x

    authors： Datta SC

    更新日期：1988-04-01 00:00:00