The alpha 1 and alpha 6 subunits can coexist in the same cerebellar GABAA receptor maintaining their individual benzodiazepine-binding specificities.


:Two GABAA receptor subunit-specific antibodies anti-alpha 6 and anti-alpha 1 have been used for elucidating the relationship between the presence of alpha 1 and/or alpha 6 subunits in the cerebellar GABAA receptors and the benzodiazepine-binding specificity. Receptor immunoprecipitation with the subunit-specific antibodies shows that 39% of the cerebellar GABAA receptors have alpha 6, whereas 76% of the receptors have alpha 1 as determined by [3H]-muscimol binding. Results show that 42-45% of the receptors having alpha 6 also have alpha 1, whereas 13-15% of the receptors that contain alpha 1 also have alpha 6. The immunoprecipitation results as well as immunopurification and immunoblotting experiments reveal the existence of three types of cerebellar GABAA receptors; i.e., one has both alpha 1 and alpha 6 subunits, a second type has alpha 1 but not alpha 6, and a third type has alpha 6 but not alpha 1 subunits. The results also show that receptors where alpha 1 and alpha 6 subunits coexist have two pharmacologically different benzodiazepine-binding properties, each associated with a different alpha subunit. The alpha 1 subunit contributes the high-affinity binding of [3H]Ro 15-1788 (flumazenil) and the diazepam-sensitive binding of [3H]Ro 15-4513. The alpha 6 subunit contributes the diazepam-insensitive binding of [3H]Ro 15-4513, but it does not bind [3H]Ro 15-1788 with high affinity. Thus, in the cerebellar alpha 1- alpha 6 GABAA receptors, there is no dominance of the pharmacology of one alpha subunit over the other.


J Neurochem


Khan ZU,Gutiérrez A,De Blas AL




Has Abstract


1996-02-01 00:00:00












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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Weyhenmeyer JA,Meyer JM,Watkins JE

    更新日期:1984-09-01 00:00:00

  • Metallothionein-III prevents glutamate and nitric oxide neurotoxicity in primary cultures of cerebellar neurons.

    abstract::Metallothionein (MT)-III, a member of the MT family of metal-binding proteins, is mainly expressed in the CNS and is abundant in glutamatergic neurons. Results in genetically altered mice indicate that MT-III may play neuroprotective roles in the brain, but the mechanisms through which this protein functions have not ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Montoliu C,Monfort P,Carrasco J,Palacios O,Capdevila M,Hidalgo J,Felipo V

    更新日期:2000-07-01 00:00:00

  • Oxidative-stress-dependent up-regulation of Bcl-2 expression in the central nervous system of aged Fisher-344 rats.

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Kaufmann JA,Bickford PC,Taglialatela G

    更新日期:2001-02-01 00:00:00

  • Mutant presenilin 1 increases the levels of Alzheimer amyloid beta-peptide Abeta42 in late compartments of the constitutive secretory pathway.

    abstract::Mutations in the presenilin 1 (PS1) gene are associated with autosomal dominant, early-onset, familial Alzheimer's disease and result in increased release of the hyperaggregatable 42-amino acid form of the amyloid beta-peptide (A(beta)42). To determine which subcellular compartments are potential source(s) of released...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Petanceska SS,Seeger M,Checler F,Gandy S

    更新日期:2000-05-01 00:00:00

  • Nidogen is a prosurvival and promigratory factor for adult Schwann cells.

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    authors: Lee HK,Seo IA,Park HK,Park YM,Ahn KJ,Yoo YH,Park HT

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    authors: King MD,Phillips GW,Bignone PA,Hayes NV,Pinder JC,Baines AJ

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Miralles A,Ribas C,Olmos G,García-Sevilla JA

    更新日期:1993-11-01 00:00:00

  • Mutual inhibition kinetic analysis of gamma-aminobutyric acid, taurine, and beta-alanine high-affinity transport into neurons and astrocytes: evidence for similarity between the taurine and beta-alanine carriers in both cell types.

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    pub_type: 杂志文章


    authors: Larsson OM,Griffiths R,Allen IC,Schousboe A

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  • Expression and evolution of the mammalian brain gene Ttyh1.

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    pub_type: 杂志文章


    authors: Matthews CA,Shaw JE,Hooper JA,Young IG,Crouch MF,Campbell HD

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: De Luca MA,Bassareo V,Bauer A,Di Chiara G

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Gordon SL,Quinsey NS,Dunkley PR,Dickson PW

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  • Role of caspases in N-methyl-D-aspartate-induced apoptosis in cerebrocortical neurons.

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Tenneti L,D'Emilia DM,Troy CM,Lipton SA

    更新日期:1998-09-01 00:00:00

  • Oxidant injury in PC12 cells--a possible model of calcium "dysregulation" in aging: I. Selectivity of protection against oxidative stress.

    abstract::Previous research has suggested that the initial effects of cellular free radical neurotoxic insult involve large increases in intracellular Ca2+. However, the exact role of oxidative stress on the various parameters involved in these increases has not been specified. The present experiments were performed to examine ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Joseph JA,Strain JG,Jimenez ND,Fisher D

    更新日期:1997-09-01 00:00:00

  • Functional mapping of dorsal and median raphe 5-hydroxytryptamine pathways in forebrain of the rat using microdialysis.

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: McQuade R,Sharp T

    更新日期:1997-08-01 00:00:00

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Rivett AJ,Francis A,Roth JA

    更新日期:1983-01-01 00:00:00

  • Alternative splicing of a 51-nucleotide exon that encodes a putative protein kinase C phosphorylation site generates two forms of the chicken gamma-aminobutyric acidA receptor beta 2 subunit.

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Harvey RJ,Chinchetru MA,Darlison MG

    更新日期:1994-01-01 00:00:00

  • Neurotransmitter receptor ligand binding and enzyme regional distribution in the pigeon visual system.

    abstract::The relative importance of acetylcholine, dopamine, endogenous opiates, gamma-aminobutyric acid (GABA), glutamate, glycine, noradrenaline, and serotonin as transmitters in the pigeon visual system was estimated by measuring the activity of choline acetyltransferase (ChAT), glutamic acid decarboxylase (GAD), and aromat...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Vischer A,Cuénod M,Henke H

    更新日期:1982-05-01 00:00:00

  • Headless Myo10 is a regulator of microtubule stability during neuronal development.

    abstract::Stabilized microtubules are required for neuronal morphogenesis and migration. However, the underlying mechanism is not fully understood. In this study, we demonstrate that myosin X (Myo10), which is composed of full-length myosin X (fMyo10) and headless myosin X (hMyo10), is important for axon development. fMyo10 is ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Yu H,Sun D,Wang N,Wang M,Lan Y,Fan W,Zhao Y,Guo W,Zhu X

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  • A sensitive method for characterization of oligoclonal immunoglobulins in unconcentrated cerebrospinal fluid.

    abstract::Although isoelectric focusing has been used to demonstrate the presence of oligoclonal IgG in the CSF, the technique has not allowed detection of oligoclonal IgG in unconcentrated CSF. A new technique is reported, by which unconcentrated CSF is separated by isoelectric focusing, and the IgG bands are then detected by ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Lasne Y,Benzerara O,Chazot G,Creyssel R

    更新日期:1981-05-01 00:00:00

  • Steroidogenic acute regulatory protein (StAR) transcripts constitutively expressed in the adult rat central nervous system: colocalization of StAR, cytochrome P-450SCC (CYP XIA1), and 3beta-hydroxysteroid dehydrogenase in the rat brain.

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    pub_type: 杂志文章


    authors: Furukawa A,Miyatake A,Ohnishi T,Ichikawa Y

    更新日期:1998-12-01 00:00:00

  • Regional distribution and age-dependent expression of N-acylphosphatidylethanolamine-hydrolyzing phospholipase D in rat brain.

    abstract::The endocannabinoid anandamide (N-arachidonoylethanolamine) and other bioactive long-chain N-acylethanolamines are thought to be formed from their corresponding N-acylphosphatidylethanolamines by a specific phospholipase D (NAPE-PLD) in the brain as well as other tissues. However, regional distribution of NAPE-PLD in ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Morishita J,Okamoto Y,Tsuboi K,Ueno M,Sakamoto H,Maekawa N,Ueda N

    更新日期:2005-08-01 00:00:00

  • The NK-1 receptor and a calcium-phospholipid pathway: inositol trisphosphate production and calcium movements induced by selective agonists of neurokinin receptors in rat parotid glands.

    abstract::In the rat parotid gland, substance P has been shown to induce a phosphatidylinositol bisphosphate breakdown resulting in an inositol trisphosphate production. These data suggested that substance P activated a phospholipase C and thus mediated its effects through the calcium-phospholipid pathway. To determine which ne...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Guillemain I,Rollandy I,Imhoff V,Rossignol B

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  • Myelin basic protein gene and the function of antisense RNA in its repression in myelin-deficient mutant mouse.

    abstract::The myelin-deficient (mld) mouse is an autosomal recessive mutant characterized by hypomyelination of the CNS due to reduced expression of the myelin basic protein (MBP) gene. In the mld mutant, the MBP gene is duplicated in tandem. One gene is intact, but a large portion is inverted upstream of the other copy, and it...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Okano H,Aruga J,Nakagawa T,Shiota C,Mikoshiba K

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  • Inhibition of stress- or anxiogenic-drug-induced increases in dopamine release in the rat prefrontal cortex by long-term treatment with antidepressant drugs.

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Dazzi L,Spiga F,Pira L,Ladu S,Vacca G,Rivano A,Jentsch JD,Biggio G

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  • Overexpression of c-Fos is sufficient to stimulate tyrosine hydroxylase (TH) gene transcription in rat pheochromocytoma PC18 cells.

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Sun B,Tank AW

    更新日期:2002-01-01 00:00:00

  • Adult neural stem cells: an endogenous tool to repair brain injury?

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章,评审


    authors: Bellenchi GC,Volpicelli F,Piscopo V,Perrone-Capano C,di Porzio U

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  • A kainate receptor linked to nitric oxide synthesis from arginine.

    abstract::In slices of young rat cerebellum, the glutamate analogue kainate induced a large accumulation of cyclic GMP, which was inhibited by non-N-methyl-D-aspartate antagonists. Quisqualate and alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate evoked only small cyclic GMP responses and inhibited the effect of kainate. Whe...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Garthwaite J,Southam E,Anderton M

    更新日期:1989-12-01 00:00:00

  • Cerebral vulnerability is associated with selective increase in extracellular glutamate concentration in experimental thiamine deficiency.

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Hazell AS,Butterworth RF,Hakim AM

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  • IL-1beta induces a MyD88-dependent and ceramide-mediated activation of Src in anterior hypothalamic neurons.

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Davis CN,Tabarean I,Gaidarova S,Behrens MM,Bartfai T

    更新日期:2006-09-01 00:00:00

  • Protein phosphotyrosine in mouse brain: developmental changes and regulation by epidermal growth factor, type I insulin-like growth factor, and insulin.

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Girault JA,Chamak B,Bertuzzi G,Tixier H,Wang JK,Pang DT,Greengard P

    更新日期:1992-02-01 00:00:00