当前位置： SCI文献检索 > JOURNAL OF NEUROCHEMISTRY期刊下所有文献 > GM1 ganglioside treatment of PC12 cells stimulates ganglioside, glycolipid, and lipid, but not glycoprotein synthesis independently from the effects of nerve growth factor.

GM1 ganglioside treatment of PC12 cells stimulates ganglioside, glycolipid, and lipid, but not glycoprotein synthesis independently from the effects of nerve growth factor.

Abstract：

:The incorporation of radioactive precursors into gangliosides and other glycolipids, glycoproteins, and total lipids has been studied in rat pheochromocytoma PC12 cells. Starting with the same PC12 cell pool, cultures displaying different degrees of neuritic expression in response to nerve growth factor (NGF) and combinations of serum ganglioside GM1 were produced. Attempts were then made to correlate neuritic regulation with biochemical performances of these cells. NGF stimulates the incorporation of [3H]galactose into gangliosides and other glycolipids and glycoproteins and [14C]acetate into total lipids, regardless of the serum concentration. NGF both increased their initial labeling rates and promoted additional and more extensive labeling from culture day 4 onward. Unexpectedly, exogenous GM1 also elicited an increase in ganglioside labeling as well as that of the other lipid classes, but not of glycoproteins. The GM1-induced increase was evident at higher serum concentrations (1%) regardless of the presence or absence of NGF, but not apparent in low (0.15%) serum. Serum levels themselves did not affect labeling patterns in the absence of NGF and GM1. GM1-induced stimulation of labeling reflects an increase in the synthetic activities of the cells, and not increased precursor uptake or reduced product degradation. For all constituents stimulated by GM1, concurrent treatment with NGF produces cumulative effects, suggesting independent mechanisms of action by the two molecules.

journal_name

J Neurochem

journal_title

Journal of neurochemistry

authors

Katoh-Semba R,Skaper SD,Varon S

doi

10.1111/j.1471-4159.1986.tb13006.x

subject

Has Abstract

pub_date

1986-02-01 00:00:00

pages

574-82

issue

2

eissn

0022-3042

issn

1471-4159

journal_volume

46

pub_type

杂志文章

相关文献

JOURNAL OF NEUROCHEMISTRY文献大全
  • Modulation of serotonin transporter activity by a protein kinase C activator and an inhibitor of type 1 and 2A serine/threonine phosphatases.

    abstract：:We studied the effects of 12-O-tetradecanoylphorbol 13-acetate (TPA), a protein kinase C (PKC) activator, and calyculin A (CLA), an inhibitor of type 1 and 2A serine/threonine phosphatases, on serotonin uptake by a human placenta choriocarcinoma cell line (BeWo) and COS-7 cells expressing recombinant serotonin transpo...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1046/j.1471-4159.1997.68062618.x

    authors： Sakai N,Sasaki K,Nakashita M,Honda S,Ikegaki N,Saito N

    更新日期：1997-06-01 00:00:00

  • [3H]1-[2-(4-aminophenyl)ethyl]-4-(3-trifluoromethylphenyl)piperazine: a selective radioligand for 5-HT1A receptors in rat brain.

    abstract：:1-[2-(4-Aminophenyl)ethyl]-4-(3-trifluoromethylphenyl)piperazine (PAPP) inhibits [3H]5-hydroxytryptamine (5-HT, serotonin) binding to 5-HT1A and 5-HT1B sites in rat brain with apparent equilibrium dissociation constants (KD) of 2.9 and 328 nM, respectively. [3H]PAPP was synthesized, its binding to central serotonin re...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.1986.tb12926.x

    authors： Ransom RW,Asarch KB,Shih JC

    更新日期：1986-01-01 00:00:00

  • Human amylin induces "apoptotic" pattern of gene expression concomitant with cortical neuronal apoptosis.

    abstract：:Amylin forms large beta-pleated neurotoxic oligomers but shows only 38% sequence similarity to A beta. As patterns of gene expression during neuronal apoptosis appear stimulus and cell type specific, we compared the pattern of amylin-induced gene expression in rat cortical neurons with that shown previously to be indu...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1046/j.1471-4159.1998.71020506.x

    authors： Tucker HM,Rydel RE,Wright S,Estus S

    更新日期：1998-08-01 00:00:00

  • Stress hormones collaborate to induce lymphocyte apoptosis after high level spinal cord injury.

    abstract：:Post-traumatic immune suppression renders individuals with spinal cord injury (SCI) susceptible to infection. Normally, proper immune function is regulated by collaboration between the sympathetic nervous system (SNS) and hypothalamic-pituitary-adrenal (HPA) axis and involves the controlled release of glucocorticoids ...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.2009.06232.x

    authors： Lucin KM,Sanders VM,Popovich PG

    更新日期：2009-09-01 00:00:00

  • Differential effects of Parkin and its mutants on protein aggregation, the ubiquitin-proteasome system, and neuronal cell death in human neuroblastoma cells.

    abstract：:Mutations in Parkin, an E3 ligase, which participates in the ubiquitin-proteasome system (UPS), cause juvenile onset Parkinson's disease (PD). Some mutants aggregate upon over-expression, but the effects of such aggregation on the UPS and neuronal survival have not been characterized. We show in this study that transi...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.2007.04620.x

    authors： Kyratzi E,Pavlaki M,Kontostavlaki D,Rideout HJ,Stefanis L

    更新日期：2007-08-01 00:00:00

  • Proteasomal inhibition leads to formation of ubiquitin/alpha-synuclein-immunoreactive inclusions in PC12 cells.

    abstract：:Proteasomal dysfunction has been recently implicated in the pathogenesis of several neurodegenerative diseases, including Parkinson's disease and diffuse Lewy body disease. We have developed an in vitro model of proteasomal dysfunction by applying pharmacological inhibitors of the proteasome, lactacystin or ZIE[O-tBu]...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1046/j.1471-4159.2001.00474.x

    authors： Rideout HJ,Larsen KE,Sulzer D,Stefanis L

    更新日期：2001-08-01 00:00:00

  • Biochemical and immunochemical studies on the GABAergic system in the rat fallopian tube and ovary.

    abstract：:gamma-Aminobutyric acid (GABA) and glutamic acid decarboxylase (GAD) activities were measured in the ovary and the Fallopian tube of rats and compared with brain values. GABA levels in the Fallopian tube were about twice as high as in the brain, while in the ovary they represented only about 5% of the amino acid conte...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.1984.tb06686.x

    authors： Apud JA,Tappaz ML,Celotti F,Negri-Cesi P,Masotto C,Racagni G

    更新日期：1984-07-01 00:00:00

  • Asp664 cleavage of amyloid precursor protein induces tau phosphorylation by decreasing protein phosphatase 2A activity.

    abstract：:Caspase cleavage of amyloid precursor protein (APP) has been reported to be important in amyloid beta protein (Aβ)-mediated neurotoxicity. However, the underlying mechanisms are not clearly understood. In this study, we explored the effect of caspase cleavage of APP on tau phosphorylation in relation to Aβ. We found t...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/jnc.12032

    authors： Park SS,Jung HJ,Kim YJ,Park TK,Kim C,Choi H,Mook-Jung IH,Koo EH,Park SA

    更新日期：2012-12-01 00:00:00

  • Agonist efficacy and receptor efficiency in heterozygous CB1 knockout mice: relationship of reduced CB1 receptor density to G-protein activation.

    abstract：:Heterozygous CB1 receptor knockout mice were used to examine the effect of reduced CB1 receptor density on G-protein activation in membranes prepared from four brain regions: cerebellum, hippocampus, striatum/globus pallidus (striatum/GP) and cingulate cortex. Results showed that CB1 receptor levels were approximately...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1046/j.1471-4159.2001.00308.x

    authors： Selley DE,Rorrer WK,Breivogel CS,Zimmer AM,Zimmer A,Martin BR,Sim-Selley LJ

    更新日期：2001-05-01 00:00:00

  • Limited predictability of postmortem human brain tissue quality by RNA integrity numbers.

    abstract：:The RNA integrity number (RIN) is often considered to be a critical measure of the quality of postmortem human brains. However, it has been suggested that RINs do not necessarily reflect the availability of intact mRNA. Using the Agilent bioanalyzer and qRT-PCR, we explored whether RINs provide a meaningful way of ass...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/jnc.13637

    authors： Sonntag KC,Tejada G,Subburaju S,Berretta S,Benes FM,Woo TU

    更新日期：2016-07-01 00:00:00

  • Modelling Parkinson-like neurodegeneration via osmotic minipump delivery of MPTP and probenecid.

    abstract：:Mouse models of MPTP intoxication have been used extensively to explore the molecular mechanisms of Parkinson's disease. However, these models present some limitations since; (i) Dopaminergic (DA) cell death occurs rapidly in contrast to the presumably slow evolution of the disease process. (ii) Some of the key histol...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.2008.05651.x

    authors： Alvarez-Fischer D,Guerreiro S,Hunot S,Saurini F,Marien M,Sokoloff P,Hirsch EC,Hartmann A,Michel PP

    更新日期：2008-11-01 00:00:00

  • Up-regulation of GLT1 reverses the deficit in cortically evoked striatal ascorbate efflux in the R6/2 mouse model of Huntington's disease.

    abstract：:A corticostriatal-dependent deficit in the release of ascorbate (AA), an antioxidant vitamin and neuromodulator, occurs concurrently in striatum with dysfunctional GLT1-dependent uptake of glutamate in the R6/2 mouse model of Huntington's disease (HD), an autosomal dominant condition characterized by overt corticostri...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.2012.07691.x

    authors： Miller BR,Dorner JL,Bunner KD,Gaither TW,Klein EL,Barton SJ,Rebec GV

    更新日期：2012-05-01 00:00:00

  • Ras-regulated hypophosphorylation of the retinoblastoma protein mediates neuronal differentiation in PC12 cells.

    abstract：:To investigate the role of the retinoblastoma protein pRB in neuronal differentiation, we have measured the accumulation of hypophosphorylated pRB in PC12 cells stimulated by nerve growth factor (NGF). NGF induced the accumulation of hypophosphorylated pRB within 30 min and the level peaked after 12 h. Viral Kiras, cy...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1046/j.1471-4159.1996.66062287.x

    authors： Li H,Kawasaki H,Nishida E,Hattori S,Nakamura S

    更新日期：1996-06-01 00:00:00

  • Therapeutic approaches in Parkinson's disease and related disorders.

    abstract：:The lack of effective therapies for neurodegenerative disorders is one of the most relevant challenges of this century, considering that, as the global population ages, the incidence of these type of diseases is quickly on the rise. Among these disorders, synucleinopathies, which are characterized by the abnormal accu...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章,评审

    doi：10.1111/jnc.13529

    authors： Valera E,Masliah E

    更新日期：2016-10-01 00:00:00

  • Myelin galactolipid synthesis in different strains of mice.

    abstract：:Previous studies have indicated that the brains of DBA/2J (D2) mice have a more heavily myelinated CNS than those of C57BL/6J (B6) at postnatal days 17-21. However, the amount of myelin in the brains of F1 (B6 X D2) hybrids is even higher than in their parental strains. To investigate further factors involved in regul...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.1987.tb09995.x

    authors： Sato C,Yu RK

    更新日期：1987-10-01 00:00:00

  • Involvement of OCTN2 and B0,+ in the transport of carnitine through an in vitro model of the blood-brain barrier.

    abstract：:Carnitine is known to accumulate in brain, therefore transport of carnitine through the blood-brain barrier was studied in an in vitro system using bovine brain capillary endothelial cells (BBCEC) grown on filter inserts in a co-culture system with glial cells. Long-term exposure of BBCEC to carnitine resulted in a hi...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.2004.02752.x

    authors： Berezowski V,Miecz D,Marszałek M,Bröer A,Bröer S,Cecchelli R,Nałecz KA

    更新日期：2004-11-01 00:00:00

  • Neurotoxic mechanisms triggered by Alzheimer's disease-linked mutant M146L presenilin 1: involvement of NO synthase via a novel pertussis toxin target.

    abstract：:While it has been reported that familial Alzheimer's disease (FAD)-linked mutants of amyloid precursor protein (APP) and presenilin (PS)2 induce neuronal cytotoxicity in a manner sensitive to antioxidant and pertussis toxin (PTX), little of the mechanism for PS1-mediated neuronal cell death has been characterized. We ...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1046/j.0022-3042.2001.00722.x

    authors： Hashimoto Y,Ito Y,Arakawa E,Kita Y,Terashita K,Niikura T,Nishimoto I

    更新日期：2002-02-01 00:00:00

  • Functional mapping of dorsal and median raphe 5-hydroxytryptamine pathways in forebrain of the rat using microdialysis.

    abstract：:Recent neurochemical studies of the properties of 5-hydroxytryptamine (5-HT) pathways arising from the dorsal raphe nucleus (DRN) and median raphe nucleus (MRN) have measured extracellular 5-HT in brain regions with reported preferential DRN or MRN 5-HT inputs. Here, we have tested whether electrical stimulation of th...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1046/j.1471-4159.1997.69020791.x

    authors： McQuade R,Sharp T

    更新日期：1997-08-01 00:00:00

  • Phosphatase activity toward abnormally phosphorylated tau: decrease in Alzheimer disease brain.

    abstract：:Microtubule-associated protein tau is abnormally hyperphosphorylated and aggregated in affected neurons of Alzheimer disease brain. This hyperphosphorylated tau can be dephosphorylated at some of the abnormal phosphorylated sites by purified protein phosphatase-1, 2A, and 2B in vitro. In the present study, we have dev...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1046/j.1471-4159.1995.65020732.x

    authors： Gong CX,Shaikh S,Wang JZ,Zaidi T,Grundke-Iqbal I,Iqbal K

    更新日期：1995-08-01 00:00:00

  • Sequential treatment of SH-SY5Y cells with retinoic acid and brain-derived neurotrophic factor gives rise to fully differentiated, neurotrophic factor-dependent, human neuron-like cells.

    abstract：:A rapid and simple procedure is presented to obtain nearly pure populations of human neuron-like cells from the SH-SY5Y neuroblastoma cell line. Sequential exposure of SH-SY5Y cells to retinoic acid and brain-derived neurotrophic factor in serum-free medium yields homogeneous populations of cells with neuronal morphol...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1046/j.1471-4159.2000.0750991.x

    authors： Encinas M,Iglesias M,Liu Y,Wang H,Muhaisen A,Ceña V,Gallego C,Comella JX

    更新日期：2000-09-01 00:00:00

  • Glutamate neurotoxicity in rat cerebellar granule cells: a major role for xanthine oxidase in oxygen radical formation.

    abstract：:To gain insight into the mechanism through which the neurotransmitter glutamate causally participates in several neurological diseases, in vitro cultured cerebellar granule cells were exposed to glutamate and oxygen radical production was investigated. To this aim, a novel procedure was developed to detect oxygen radi...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1046/j.1471-4159.1997.68052038.x

    authors： Atlante A,Gagliardi S,Minervini GM,Ciotti MT,Marra E,Calissano P

    更新日期：1997-05-01 00:00:00

  • Postnatal development of cholecystokinin-like immunoreactivity and its mRNA level in rat brain regions.

    abstract：:Developmental changes of preprocholecystokinin mRNA (CCK mRNA) and cholecystokinin-like immunoreactivity (CCK-LI) were examined in rat brain regions (frontal cortex, colliculi, hippocampus, striatum, and cerebellum) using RNA dot blot assays with cholecystokinin (CCK) cDNA and radioimmunoassay, respectively. The CCK-L...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.1989.tb11772.x

    authors： Takeda K,Koshimoto H,Uchiumi F,Haun RS,Dixon JE,Kato T

    更新日期：1989-09-01 00:00:00

  • Corticosterone targets distinct steps of synaptic transmission via concentration specific activation of mineralocorticoid and glucocorticoid receptors.

    abstract：:Hippocampal neurons are affected by chronic stress and have a high density of cytoplasmic mineralocorticoid and glucocorticoid receptors (MR and GR). Detailed studies on the genomic effects of the stress hormone corticosterone at physiologically relevant concentrations on different steps in synaptic transmission are l...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/jnc.12478

    authors： Chatterjee S,Sikdar SK

    更新日期：2014-02-01 00:00:00

  • The chemokine interleukin-8 acutely reduces Ca(2+) currents in identified cholinergic septal neurons expressing CXCR1 and CXCR2 receptor mRNAs.

    abstract：:The chemokine IL-8 is known to be synthesized by glial cells in the brain. It has traditionally been shown to have an important role in neuroinflammation but recent evidence indicates that it may also be involved in rapid signaling in neurons. We investigated how IL-8 participates in rapid neuronal signaling by using ...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1046/j.1471-4159.2001.00469.x

    authors： Puma C,Danik M,Quirion R,Ramon F,Williams S

    更新日期：2001-09-01 00:00:00

  • Effects of ATP and taurine on calcium uptake by membrane preparations of the rat retina.

    abstract：:The effects of ATP and taurine on the kinetics of calcium uptake in rat retinal membrane preparations were determined. ATP increased calcium uptake at low calcium ion concentrations. Addition of ATP plus taurine further increased calcium uptake. Cooperative relationships were observed for calcium uptake in the absence...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.1983.tb11296.x

    authors： Lombardini JB

    更新日期：1983-02-01 00:00:00

  • Neurofilament-light increases the cell surface expression of the N-methyl-D-aspartate receptor and prevents its ubiquitination.

    abstract：:NMDA (N-methyl-D-aspartate) subtype of glutamate receptors are core components of dendritic spine postsynaptic densities (PSDs), in which they are anchored via their carboxy-terminal tails to cytoskeletal proteins. In this study, we examined the role of the neuronal intermediate filament protein, neurofilament-light (...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.2004.02936.x

    authors： Ratnam J,Teichberg VI

    更新日期：2005-02-01 00:00:00

  • Co-expression of vesicular glutamate transporters (VGLUT1 and VGLUT2) and their association with synaptic-like microvesicles in rat pinealocytes.

    abstract：:A vesicular glutamate transporter (VGLUT) is responsible for the accumulation of l-glutamate in synaptic vesicles in glutamatergic neurons. Two isoforms, VGLUT1 and VGLUT2, have been identified, which are complementarily expressed in these neurons. Mammalian pinealocytes, endocrine cells for melatonin, are also glutam...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1046/j.1471-4159.2003.01532.x

    authors： Morimoto R,Hayashi M,Yatsushiro S,Otsuka M,Yamamoto A,Moriyama Y

    更新日期：2003-01-01 00:00:00

  • Neuro-inflammation induced in the hippocampus of 'binge drinking' rats may be mediated by elevated extracellular glutamate content.

    abstract：:The neuropathological and immune changes induced in the brain by 'binge drinking' have been investigated in a rat model. Evidence of neuro-inflammation was identified in the 'binge drinking' rat model of alcohol abuse after 3 weeks of administration of 2 or 3 g/kg ethanol (EtOH), three times per day for two consecutiv...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.2009.06389.x

    authors： Ward RJ,Colivicchi MA,Allen R,Schol F,Lallemand F,de Witte P,Ballini C,Corte LD,Dexter D

    更新日期：2009-12-01 00:00:00

  • Inhibition of glutamate-induced delayed calcium deregulation by 2-APB and La3+ in cultured cortical neurones.

    abstract：:Exposure of neurones in culture to excitotoxic levels of glutamate results in an initial transient spike in [Ca2+]i followed by a delayed, irreversible [Ca2+]i rise governed by rapid kinetics, with Ca2+ originating from the extracellular medium. The molecular mechanism responsible for the secondary Ca2+ rise is unknow...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1111/j.1471-4159.2004.02732.x

    authors： Chinopoulos C,Gerencser AA,Doczi J,Fiskum G,Adam-Vizi V

    更新日期：2004-10-01 00:00:00

  • Feedback control of mesolimbic somatodendritic dopamine release in rat brain.

    abstract：:The objective of this study was to examine the role of dopamine (DA) receptors in the nucleus accumbens (ACB) in controlling feedback regulation of mesolimbic somatodendritic DA release in the ventral tegmental area (VTA) of Wistar rats using ipsilateral dual-probe in vivo microdialysis. Perfusion of the ACB for 60 mi...

    journal_title：Journal of neurochemistry

    pub_type： 杂志文章

    doi：10.1046/j.1471-4159.2000.740684.x

    authors： Rahman S,McBride WJ

    更新日期：2000-02-01 00:00:00