Co-expression of vesicular glutamate transporters (VGLUT1 and VGLUT2) and their association with synaptic-like microvesicles in rat pinealocytes.

abstract：:Converging lines of evidence implicate the beta-amyloid peptide (Ass) as causative in Alzheimer's disease. We describe a novel class of compounds that reduce A beta production by functionally inhibiting gamma-secretase, the activity responsible for the carboxy-terminal cleavage required for A beta production. These mo...

journal_title：Journal of neurochemistry

authors： Dovey HF,John V,Anderson JP,Chen LZ,de Saint Andrieu P,Fang LY,Freedman SB,Folmer B,Goldbach E,Holsztynska EJ,Hu KL,Johnson-Wood KL,Kennedy SL,Kholodenko D,Knops JE,Latimer LH,Lee M,Liao Z,Lieberburg IM,Motter RN,

更新日期：2001-01-01 00:00:00

abstract：:Effects of concanavalin A on transmitter release were investigated in primary cultures of chick sympathetic neurons. The lectin reduced electrically evoked [3H]noradrenaline release by up to 30% with half-maximal inhibition at 0.16 microM. Concanavalin A also reduced the release triggered by extracellular Ca2+ in neur...

journal_title：Journal of neurochemistry

authors： Boehm S,Huck S

更新日期：1998-12-01 00:00:00

abstract：:CNS synapses are produced rapidly upon pre- and post-synaptic recruitment. However, their composition is known to change during development and we reasoned that this may be reflected in the gross biochemical properties of synapses. We found synaptic structure in adult cortical synaptosomes to be resistant to digestion...

journal_title：Journal of neurochemistry

authors： Khaing ZZ,Fidler L,Nandy N,Phillips GR

更新日期：2006-07-01 00:00:00

abstract：:The genetic defect in Friedreich's ataxia (FRDA) is the expansion of a GAA·TCC triplet in the first intron of the FXN gene, which encodes the mitochondrial protein frataxin. Previous studies have established that the repeats reduce transcription of this essential gene, with a concomitant decrease in frataxin protein i...

journal_title：Journal of neurochemistry

authors： Gottesfeld JM,Rusche JR,Pandolfo M

更新日期：2013-08-01 00:00:00

abstract：:Methods are described for the determination of sheep spinal cord tissue fluid content and norepinephrine, serotonin, and dopamine concentrations after experimental injury. The amount of tissue fluid varied in different regions of sheep spinal cord following injury. Norepinephrine and serotonin wet weight concentration...

journal_title：Journal of neurochemistry

authors： Hinwood BG,Yeo JD,Kidman AD

更新日期：1980-10-01 00:00:00

abstract：:In situ phosphorylation of the presynaptic protein kinase C substrate B-50 was investigated in rat hippocampal slices incubated with the convulsant drug 4-aminopyridine (4-AP). Phosphorylation of B-50 was significantly enhanced 1 min after the addition of 4-AP (100 microM). This increase by 4-AP was concentration depe...

journal_title：Journal of neurochemistry

authors： Heemskerk FM,Schrama LH,Gianotti C,Spierenburg H,Versteeg DH,De Graan PN,Gispen WH

更新日期：1990-03-01 00:00:00

abstract：:A clone of murine neuroblastoma (N1E-115) was shown to have functional receptors for the nonapeptide bradykinin. These receptors mediated a large, rapid (about 1 min to peak) and calcium-dependent increase in cyclic GMP. The median effective concentration (EC50) averaged 1.4 nM. In addition, this event was inhibited b...

journal_title：Journal of neurochemistry

authors： Snider RM,Richelson E

更新日期：1984-12-01 00:00:00

abstract：:We have proposed that a very early step in the pathogenesis of idiopathic Parkinson's disease is the elevated translocation of L-cysteine into neuromelanin-pigmented dopaminergic neurons in the substantia nigra. This influx of L-cysteine was proposed to divert the normal neuromelanin pathway by scavenging dopamine-o-q...

journal_title：Journal of neurochemistry

authors： Li H,Shen XM,Dryhurst G

更新日期：1998-11-01 00:00:00

abstract：:Rotating disk electrode voltammetry at glassy carbon electrodes and ultraviolet/visible spectroscopy were used to demonstrate that dopamine binds to neurotensin with a dissociation constant of 7.5 x 10(-8). By measuring the binding constants of various neurotensin analogs, it was found that the -Arg8-Arg9-portion of t...

journal_title：Journal of neurochemistry

authors： Adachi DK,Kalivas PW,Schenk JO

更新日期：1990-04-01 00:00:00

abstract：:Mitochondrial dysfunction and oxidative stress are known to occur following acute seizure activity but their contribution during epileptogenesis is largely unknown. The goal of this study was to determine the extent of mitochondrial oxidative stress, changes to redox status, and mitochondrial DNA (mtDNA) damage during...

journal_title：Journal of neurochemistry

authors： Waldbaum S,Liang LP,Patel M

更新日期：2010-12-01 00:00:00

abstract：:Urotensin-II (U-II), a peptide with multiple vascular effects, is detected in cholinergic neurons of the rat brainstem and spinal cord. Here, the effects of U-II on [Ca2+]i was examined in dissociated rat spinal cord neurons by fura 2 microfluorimetry. The neurons investigated were choline acetyltransferase-positive a...

journal_title：Journal of neurochemistry

authors： Filipeanu CM,Brailoiu E,Le Dun S,Dun NJ

更新日期：2002-11-01 00:00:00

abstract：:Synaptic scaffolding molecule (S-SCAM) is a synaptic protein that consists of PDZ domains, a guanylate kinase domain, and WW domains. It interacts with N-methyl-d-aspartate receptor subunits, neuroligin, and beta-catenin. Here, we identified Axin as a novel binding partner of S-SCAM. Axin was co-immunoprecipitated wit...

journal_title：Journal of neurochemistry

authors： Hirabayashi S,Nishimura W,Iida J,Kansaku A,Kishida S,Kikuchi A,Tanaka N,Hata Y

更新日期：2004-07-01 00:00:00

abstract：:It is well established that inflammatory changes contribute to brain ageing, and an increased concentration of proinflammatory cytokine, interleukin-1beta (IL-1beta), has been reported in the aged brain associated with a deficit in long-term potentiation (LTP) in rat hippocampus. The precise age at which changes are i...

journal_title：Journal of neurochemistry

authors： Griffin R,Nally R,Nolan Y,McCartney Y,Linden J,Lynch MA

更新日期：2006-11-01 00:00:00

abstract：:We reported earlier that adenine nucleotides and adenosine inhibit acetylcholine-induced catecholamine secretion from bovine adrenal medulla chromaffin cells. In this article, we used an adenosine analogue, N6-L-phenylisopropyladenosine (PIA), to study the mechanism underlying inhibition of catecholamine secretion by ...

journal_title：Journal of neurochemistry

authors： Chern YJ,Bott M,Chu PJ,Lin YJ,Kao LS,Westhead EW

更新日期：1992-10-01 00:00:00

abstract：:The endogenous neuropeptide N-acetyl-L-aspartyl-L-glutamate (NAAG) fulfills several criteria required to be accepted as a neurotransmitter. NAAG inactivation may proceed through enzymatic hydrolysis into N-acetyl-L-aspartate and glutamate by an N-acetylated-alpha-linked acidic dipeptidase (NAALADase). Therefore, some ...

journal_title：Journal of neurochemistry

authors： Serval V,Barbeito L,Pittaluga A,Cheramy A,Lavielle S,Glowinski J

更新日期：1990-07-01 00:00:00

abstract：:Proteoglycans (PGs) are major constituents of the extracellular matrix and have recently been proposed to contribute to synaptic plasticity. Hippocampal PGs have not yet been studied or linked to memory. The aim of the study, therefore, was to isolate and characterize rat hippocampal PGs and determine their possible r...

journal_title：Journal of neurochemistry

authors： Saroja SR,Sase A,Kircher SG,Wan J,Berger J,Höger H,Pollak A,Lubec G

更新日期：2014-09-01 00:00:00

abstract：:Nitric oxide (.NO) synthase (NOS) was induced in cultured rat astrocytes by incubation with lipopolysaccharide (LPS) for 18 h and gap junction permeability was assessed by the scrape-loading/Lucifer yellow transfer technique. Induction of NOS was confirmed by determining either the NG-methyl-L-arginine (NMMA)-inhibita...

journal_title：Journal of neurochemistry

authors： Bolaños JP,Medina JM

更新日期：1996-05-01 00:00:00

abstract：:A rapid and simple chromatographic procedure using HPLC with electrochemical detection is described for simultaneous determination of the substrates from precursor amino acids to metabolites related to synthesis and metabolism of three monoamine neurotransmitters--norepinephrine (NE), dopamine (DA), and 5-hydroxytrypt...

journal_title：Journal of neurochemistry

authors： Murai S,Saito H,Masuda Y,Itoh T

更新日期：1988-02-01 00:00:00

abstract：:We previously reported that annexin V promoted the survival of cultured rat neocortical neurons. In an effort to elucidate the mechanism underlying this neurotrophic activity of annexin V, we have attempted to identify the target or binding proteins of annexin V in neuronal cells. Herein, we screened an embryonic day ...

journal_title：Journal of neurochemistry

authors： Ohsawa K,Imai Y,Ito D,Kohsaka S

更新日期：1996-07-01 00:00:00

abstract：:Glutamate or a related excitatory amino acid is thought to be the major excitatory neurotransmitter of hippocampal afferents, intrinsic neurons, and efferents. We have used an autoradiographic technique to investigate the status of excitatory amino acid receptors in the hippocampal formation of patients dying with dem...

journal_title：Journal of neurochemistry

authors： Greenamyre JT,Penney JB,D'Amato CJ,Young AB

更新日期：1987-02-01 00:00:00

abstract：:RIC-3 enhances the functional expression of certain nicotinic acetylcholine receptors (nAChRs) in vertebrates and invertebrates and increases the availability of functional receptors in cultured cells and Xenopus laevis oocytes. Maximal activity of RIC-3 may be cell-type dependent, so neither mammalian nor invertebrat...

journal_title：Journal of neurochemistry

authors： Bennett HM,Lees K,Harper KM,Jones AK,Sattelle DB,Wonnacott S,Wolstenholme AJ

更新日期：2012-12-01 00:00:00

abstract：:It is well-known that the extracellular concentration of calcium affects neuronal excitability and synaptic transmission. Less is known about the physiological concentration of extracellular calcium in the brain. In electrophysiological brain slice experiments, the artificial cerebrospinal fluid traditionally contains...

journal_title：Journal of neurochemistry

authors： Forsberg M,Seth H,Björefeldt A,Lyckenvik T,Andersson M,Wasling P,Zetterberg H,Hanse E

更新日期：2019-05-01 00:00:00

abstract：:A cDNA clone, pCP9, has been isolated from a common carp liver cDNA library by immunoscreening with polyclonal antiserum raised against purified bighead carp alpha 1-antitrypsin. This clone is 1,396 bp in length and has an open reading frame encoding a protein of 410 amino acid residues. The deduced amino acid sequenc...

journal_title：Journal of neurochemistry

authors： Huang CJ,Lee MS,Huang FL,Chang GD

更新日期：1995-04-01 00:00:00

abstract：:Challenge of protease-activated receptors induces cytosolic Ca(2+) concentration ([Ca(2+) ](c)) increase, mitogen-activated protein kinase activation and reactive oxygen species (ROS) formation with a bandwidth of responses in individual cells. We detected in this study in situ the thrombin-induced [Ca(2+) ](c) rise a...

journal_title：Journal of neurochemistry

authors： Zündorf G,Reiser G

更新日期：2011-12-01 00:00:00

abstract：:An enzyme-linked immunoadsorbent assay (ELISA) for neurofilament protein was utilised to quantify the effect of exogenous ganglioside on neurite regeneration in cultures of dorsal root ganglion neurones. In contrast to nerve growth factor (NGF), ganglioside GM1 (100 micrograms/ml) failed to support neuronal survival a...

journal_title：Journal of neurochemistry

authors： Doherty P,Dickson JG,Flanigan TP,Walsh FS

更新日期：1985-04-01 00:00:00

abstract：:Most biological effects of tissue plasminogen activator (tPA), such as fibrinolysis, are mediated by its protease activity. Recent studies, however, have demonstrated that tPA also has several protease-independent effects such as: neuroprotection, microglial activation, and promoting LTP formation. In order to gain a ...

journal_title：Journal of neurochemistry

authors： Lee HY,Hwang IY,Im H,Koh JY,Kim YH

更新日期：2007-06-01 00:00:00

abstract：:The extracellular matrix (ECM) is important for both structural integrity and functions of the brain. Matrix metalloproteinases (MMPs) play major roles in ECM-remodeling under both physiological and pathological conditions. Reversion-inducing cysteine-rich protein with Kazal motifs (Reck) is a membrane-anchored MMP-re...

journal_title：Journal of neurochemistry

authors： Wang H,Imamura Y,Ishibashi R,Chandana EP,Yamamoto M,Noda M

更新日期：2010-10-01 00:00:00

abstract：:The time course of changes in extracellular glutamic acid levels and their Ca2+ dependency were studied in the rat striatum during focal cerebral ischaemia, using microdialysis. Ischaemia-induced changes were compared with those produced by high K(+)-evoked local depolarization. To optimize time resolution, glutamate ...

journal_title：Journal of neurochemistry

authors： Wahl F,Obrenovitch TP,Hardy AM,Plotkine M,Boulu R,Symon L

更新日期：1994-09-01 00:00:00

abstract：:Glutathione-S-transferase activity in the brain of male mammals (rat and mouse) was found to be relatively lower than in that of females. In contrast, the male aves (pigeon, kite, vulture, and crow) exhibited comparatively higher activity in brain glutathione-S-transferase than the corresponding females. Postnatal dev...

journal_title：Journal of neurochemistry

authors： Das M,Dixit R,Seth PK,Mukhtar H

更新日期：1981-04-01 00:00:00

abstract：:Mitochondria are important in the pathophysiology of several neurodegenerative diseases, and mitochondrial production of reactive oxygen species (ROS), membrane depolarization, permeability changes and release of apoptogenic proteins are involved in these processes. Following brain insults, cell death often occurs in ...

journal_title：Journal of neurochemistry

authors： Mattiasson G,Friberg H,Hansson M,Elmér E,Wieloch T

更新日期：2003-10-01 00:00:00