Rapid determination of norepinephrine, dopamine, serotonin, their precursor amino acids, and related metabolites in discrete brain areas of mice within ten minutes by HPLC with electrochemical detection.

abstract：:Stem cell-based approaches provide hope as a potential therapy for neurodegenerative diseases and stroke. One of the major scientific hurdles for stem cell therapy is the poor survival rate of the newly formed or transplanted neural stem cells. In this study, we found that low-dose treatment with the Heat shock protei...

journal_title：Journal of neurochemistry

authors： Wang G,Krishnamurthy K,Tangpisuthipongsa D

更新日期：2011-05-01 00:00:00

abstract：:Primary cultures of astrocytes from newborn rat brain showed evidence of a substrate-saturable process for glucose transport. The system shows a relatively high affinity for the substrate, with an apparent Km of approximately 1 mM. Maintenance of the cells in medium containing thyroid-hormone-free serum for 3, 6, or 9...

journal_title：Journal of neurochemistry

authors： Roeder LM,Williams IB,Tildon JT

更新日期：1985-11-01 00:00:00

abstract：:We investigated the role of mitogen-activated protein kinase (MAPK) pathways in hypoxic neuronal injury using primary cultures from murine cerebral cortex. Hypoxia caused the death of approximately 50% of neurons at 16 h and approximately 65% of neurons at 24 h. This was associated with phospho-activation of the MAPK/...

journal_title：Journal of neurochemistry

authors： Jin K,Mao XO,Zhu Y,Greenberg DA

更新日期：2002-01-01 00:00:00

abstract：:To study potential biochemical correlates of dopamine (DA) and serotonin receptor supersensitivity, rats were lesioned at 3 days after birth with 6-hydroxydopamine (6-OHDA; 67 micrograms in each lateral ventricle; desipramine pretreatment, 20 mg/kg i.p., 1 h) and then sensitized with the DA D1 agonist, SKF 38393 HCl (...

journal_title：Journal of neurochemistry

authors： Gong L,Kostrzewa RM,Li C

更新日期：1994-10-01 00:00:00

abstract：:Neuron-secreted factors induce astrocytic expression of the glutamate transporter, GLT-1 (excitatory amino acid transporter 2). In addition to their elaborate anatomic relationships with neurons, astrocytes also have processes that extend to and envelop the vasculature. Although previous studies have demonstrated that...

journal_title：Journal of neurochemistry

authors： Lee ML,Martinez-Lozada Z,Krizman EN,Robinson MB

更新日期：2017-12-01 00:00:00

abstract：:Serotonin-1A (5-HT(1A) receptors in the dorsal raphe nucleus (DRN) function as somatodendritic autoreceptors, and therefore play a critical role in controlling serotonergic cell firing and serotonergic neurotransmission. We hypothesized that a decrease in the capacity of 5-HT(1A) receptors to activate G proteins was a...

journal_title：Journal of neurochemistry

authors： Rossi DV,Burke TF,McCasland M,Hensler JG

更新日期：2008-05-01 00:00:00

abstract：:Three serine residues (Ser193, Ser194, Ser197) in the fifth transmembrane-spanning region of the D2 dopamine receptor have been mutated separately to alanine and the effects of the mutations determined in ligand-binding experiments with [3H] spiperone. For many antagonists the mutations had little effect, showing that...

journal_title：Journal of neurochemistry

authors： Woodward R,Coley C,Daniell S,Naylor LH,Strange PG

更新日期：1996-01-01 00:00:00

abstract：:We have found NAD to be rapidly degraded by extracellular enzymes present on intact rat brain synaptosomes. The enzyme involved had the specificity of an NADase cleaving the molecule at the nicotinamide-glycoside linkage and was inhibited by nicotinamide mononucleotide (NMN). This inhibitor did not displace specific b...

journal_title：Journal of neurochemistry

authors： Snell CR,Snell PH,Richards CD

更新日期：1984-12-01 00:00:00

abstract：:Autism is a behaviorally defined neurodevelopmental disorder and among its symptoms are disturbances in face and emotional processing. Emerging evidence demonstrates abnormalities in the GABAergic (gamma-aminobutyric acid) system in autism, which likely contributes to these deficits. GABA(B) receptors play an importan...

journal_title：Journal of neurochemistry

authors： Oblak AL,Gibbs TT,Blatt GJ

更新日期：2010-09-01 00:00:00

abstract：:In contrast to typical neuroleptic drugs, which have high affinities for dopamine D2 receptors, clozapine binds to multiple neurotransmitter receptors. The mechanisms responsible for its superior clinical efficacy over typical neuroleptics remain unknown. Using an automated genomics approach, total gene expression ana...

journal_title：Journal of neurochemistry

authors： Thomas EA,Danielson PE,Nelson PA,Pribyl TM,Hilbush BS,Hasel KW,Sutcliffe JG

更新日期：2001-02-01 00:00:00

abstract：:Autoimmune optic neuritis (AON), a model of multiple sclerosis-associated optic neuritis, is accompanied by degeneration of retinal ganglion cells (RGCs) and optic nerve demyelination and axonal loss. In order to investigate the role of N-methyl-d-aspartate (NMDA) receptors in mediating RGC degeneration, upstream chan...

journal_title：Journal of neurochemistry

authors： Bojcevski J,Stojic A,Hoffmann DB,Williams SK,Müller A,Diem R,Fairless R

更新日期：2020-06-01 00:00:00

abstract：:The MRC OX-2 monoclonal antibody recognises membrane glycoproteins of Mr 41,000 in rat brain and 47,000 on thymocytes. It also reacts with follicular dendritic cells in lymphoid organs, endothelium, smooth muscle, and B-lymphocytes. Indirect immunoperoxidase staining of cryostat sections showed that OX-2 antigen was p...

journal_title：Journal of neurochemistry

authors： Webb M,Barclay AN

更新日期：1984-10-01 00:00:00

abstract：:Adrenomedullary chromaffin cells represent an excellent model to study the molecular events linked to exocytosis, because they use the same type of SNAREs for vesicle docking and fusion as neurons. In these cells, both in the intact tissue and in isolated cells in culture, syntaxin-1 and SNAP-25 are present in the pla...

journal_title：Journal of neurochemistry

authors： López-Font I,Torregrosa-Hetland CJ,Villanueva J,Gutiérrez LM

更新日期：2010-09-01 00:00:00

abstract：:In all the species examined thus far, the behavior of microglia during development appears to be highly stereotyped. This reproducibility supports the notion that these cells have a physiological role in development. Microglia are macrophages that migrate from the yolk sac and colonize the central nervous system early...

journal_title：Journal of neurochemistry

authors： Pont-Lezica L,Béchade C,Belarif-Cantaut Y,Pascual O,Bessis A

更新日期：2011-12-01 00:00:00

abstract：:The aim of the present study was to explore the possible role of kappa/dynorphin system in the development of tolerance to nicotine antinociception in mice. First, we observed that kappa-opioid receptor (KOP-r) participates in the acute spinal antinociception produced by nicotine (3 and 5 mg/kg, s.c.) since the pre-tr...

journal_title：Journal of neurochemistry

authors： Galeote L,Maldonado R,Berrendero F

更新日期：2008-05-01 00:00:00

abstract：:Using ligand binding techniques, we studied beta-adrenergic receptor subtypes in brains obtained at autopsy from seven histologically normal controls and seven histopathologically verified cases with Alzheimer-type dementia (ATD). Inhibition of [3H]dihydroalprenolol [( 3H]DHA) binding by the selective beta 1 antagonis...

journal_title：Journal of neurochemistry

authors： Shimohama S,Taniguchi T,Fujiwara M,Kameyama M

更新日期：1987-04-01 00:00:00

abstract：:According to the amyloid hypothesis, accumulation of amyloid beta-peptide (A beta) in the brain is the primary pathogenic event in Alzheimer's disease (AD). Recent evidence indicates that A beta within the intravascular space is linked to A beta deposited in the brain suggesting that transport of A beta between the br...

journal_title：Journal of neurochemistry

authors： Zlokovic BV

更新日期：2004-05-01 00:00:00

abstract：:Our gut harbours trillions of microorganisms essential for the maintenance of homeostasis and host physiology in health and disease. In the last decade, there has been a growing interest in understanding the bidirectional pathway of communication between our microbiota and the central nervous system. With regard to re...

journal_title：Journal of neurochemistry

authors： García-Cabrerizo R,Carbia C,O Riordan KJ,Schellekens H,Cryan JF

更新日期：2020-12-25 00:00:00

abstract：:Previous work from our laboratory points to plasma free tryptophan being a useful predictor of brain tryptophan concentration in many circumstances. Other work, in particular various studies on the acute effects of food intake, has emphasized the roles of plasma total tryptophan and of plasma large neutral amino acids...

journal_title：Journal of neurochemistry

authors： Sarna GS,Kantamaneni BD,Curzon G

更新日期：1985-05-01 00:00:00

abstract：:We describe here two types of apoptotic cell death observed in the rat CNS-derived neuroblastoma B50 and B104 cells. One type was induced by dibutyryl cyclic AMP (DBcAMP) after differentiation, and the other was induced by treatment of proliferating cells with cycloheximide. When B50 and B104 cells were treated with 1...

journal_title：Journal of neurochemistry

authors： Honma N,Uchida A,Hirose H,Srsen V,Kishimoto T,Hisanaga S

更新日期：1996-11-01 00:00:00

abstract：:Excessive N-methyl-D-aspartate (NMDA) signaling is thought to contribute to bipolar disorder symptoms. Lithium and carbamazepine, effective against bipolar mania, are reported in rats to reduce brain transcription of an arachidonic acid selective calcium-dependent cytosolic phospholipase A(2) (cPLA(2)), as well as exp...

journal_title：Journal of neurochemistry

authors： Rao JS,Ertley RN,Rapoport SI,Bazinet RP,Lee HJ

更新日期：2007-09-01 00:00:00

abstract：:Glycine residues can introduce flexibility in proteins, give rise to turns and breaks in secondary structure and are key components of some nucleotide binding motifs. In the P2X receptor extracellular ATP binding domain, 11 glycine residues are completely conserved and an additional five are conserved in at least five...

journal_title：Journal of neurochemistry

authors： Digby HR,Roberts JA,Sutcliffe MJ,Evans RJ

更新日期：2005-12-01 00:00:00

abstract：:Leptin is well known as a hormone important in the central control of appetitive behaviors via receptor-mediated actions in the hypothalamus, where leptin adjusts food intake to maintain homeostasis with the body's energy stores. Recent evidence has shown that leptin and its receptors are widespread in the CNS and may...

journal_title：Journal of neurochemistry

authors： Signore AP,Zhang F,Weng Z,Gao Y,Chen J

更新日期：2008-09-01 00:00:00

abstract：:In frozen-thawed repeatedly washed rat cortical synaptic membranes, Ca2+ (1-5 mM) decreased the binding of [3H]muscimol whereas it increased the binding of [3H]gamma-aminobutyric acid (GABA). However, the binding of [3H]GABA was decreased by the same extent as the binding of [3H]muscimol when the membranes were incuba...

journal_title：Journal of neurochemistry

authors： Corda MG,Guidotti A

更新日期：1983-07-01 00:00:00

abstract：:Proper brain function in the nervous system relies on the accurate establishment of synaptic contacts during development. Countless synapses populate the adult brain in an orderly fashion. In each synapse, a presynaptic terminal loaded with neurotransmitters-containing synaptic vesicles is perfectly aligned to an arra...

journal_title：Journal of neurochemistry

authors： Pinto MJ,Almeida RD

更新日期：2016-12-01 00:00:00

abstract：:We have identified a novel mouse gene named pancortin that is expressed dominantly in the mature cerebral cortex. This gene produces four different species of proteins, Pancortin-1-4, sharing a common region in the middle of their structure with two variations at the N-terminal (A1 or A2 part) and C-terminal (C1 or C2...

journal_title：Journal of neurochemistry

authors： Nagano T,Nakamura A,Konno D,Kurata M,Yagi H,Sato M

更新日期：2000-07-01 00:00:00

abstract：:A reversed-phase HPLC system was used to concentrate and separate components of substance P-like immunoreactivity (SP-LI) from human CSF. When CSF was injected and fractions collected, no SP-LI could be detected by radioimmunoassay (RIA) at the retention time of SP or SP-sulfoxide. Instead, SP-LI was detected in later...

journal_title：Journal of neurochemistry

authors： Toresson G,Brodin E,Wahlström A,Bertilsson L

更新日期：1988-06-01 00:00:00

abstract：:In the rat parotid gland, substance P has been shown to induce a phosphatidylinositol bisphosphate breakdown resulting in an inositol trisphosphate production. These data suggested that substance P activated a phospholipase C and thus mediated its effects through the calcium-phospholipid pathway. To determine which ne...

journal_title：Journal of neurochemistry

authors： Guillemain I,Rollandy I,Imhoff V,Rossignol B

更新日期：1992-06-01 00:00:00

abstract：:Previous studies have shown that phorbol esters and lithium each stimulate the secretion of adrenocorticotropic hormone (ACTH) by the anterior pituitary tumor cell line AtT20/D16-16. Pretreatment with either lithium or phorbol ester desensitizes the cells to subsequent stimulation by phorbol ester. An early consequenc...

journal_title：Journal of neurochemistry

authors： Zatz M,Mahan LC,Reisine T

更新日期：1987-01-01 00:00:00

abstract：:Ca2+ uptake into the endoplasmic reticulum (ER) is mediated by Ca2+ ATPase isoforms, which are all selectively inhibited by nanomolar concentrations of thapsigargin. Using ATP/Mg2+-dependent 45Ca2+ transport in rat brain microsomes, tissue sections, and permeabilized cells, as well as Ca2+ imaging in living cells we d...

journal_title：Journal of neurochemistry

authors： Watson WD,Facchina SL,Grimaldi M,Verma A

更新日期：2003-10-01 00:00:00