The anti-apoptosis protein, survivin, mediates gastric epithelial cell cytoprotection against ethanol-induced injury via activation of the p34(cdc2) cyclin-dependent kinase.


:The anti-apoptosis protein, survivin, promotes cell survival and mitosis. Recent studies have demonstrated that survivin is expressed in normal gastric mucosa. Using an in vitro model, we examined whether survivin plays a role in the cytoprotection produced in gastric mucosa by mild irritant ethanol (ETOH) against subsequent exposure to concentrated ETOH. Pre-treatment of rat gastric epithelial cells with 1% ETOH reduced cell death, in response to subsequent incubation with 5% ETOH, by 94% (P < 0.005). This pre-treatment also resulted in increased total and phosphorylated survivin protein levels by 180% (P < 0.0001) and 540% (P < 0.0002), respectively, which required the p34(cdc2) cell cycle-dependent kinase. The cytoprotective effect was abrogated upon siRNA knockdown of survivin protein levels. Further, overexpression of exogenous survivin resulted in significant cytoprotection by 62% (P < 0.02) in the absence of any pre-treatment. We further examined the in vivo relevance of these findings. In fasted rats, administration of 20% ETOH, which we found to be 93% (P < 0.0001) cytoprotective against 50% ETOH challenge, resulted in increased total and phosphorylated survivin protein levels by 234% (P < 0.001) and 214% (P < 0.02), respectively. Administration of 20% ETOH resulted in increased gastric p34(cdc2) activity by 146% (P < 0.01). Inhibition of p34(cdc2) by the potent inhibitor, roscovitine, abolished the increased survivin levels in response to pre-administration of 20% ETOH and reduced the cytoprotection against 50% ETOH challenge by 59% (P < 0.01). These results indicate that survivin is a key mediator of cytoprotection against ETOH-induced gastric injury, acting at the epithelial cell level, by a mechanism that is dependent, in part, on p34(cdc2).


J Cell Physiol


Jones MK,Padilla OR,Webb NA,Norng M




Has Abstract


2008-06-01 00:00:00












  • Early changes in the membrane of HeLa cells adsorbing Sendai virus under conditions of fusion.

    abstract::Adsorption of Sendai virus at high multiplicity (500-1,000 HAU/10(6) cells) to HeLa cells grown in monolayers causes immediate changes in the ion barrier of the cell membrane, as well as changes in the morphology of the virus-treated cells. Within minutes of adsorption the cells begin to lose potassium and an extensiv...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Spiegelstein PF,Haimsohn M,Gitelman J,Kohn A

    更新日期:1978-05-01 00:00:00

  • High glucose alters the secretome of mechanically stimulated osteocyte-like cells affecting osteoclast precursor recruitment and differentiation.

    abstract::Diabetes mellitus (DM) induces bone deterioration, while mechanical stimulation promotes osteocyte-driven bone formation. We aimed to evaluate the interaction of acute exposure (24 h) to high glucose (HG) with both the pro-survival effect conferred to osteocytic MLO-Y4 cells and osteoblastic MC3T3-E1 cells by mechanic...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Maycas M,Portolés MT,Matesanz MC,Buendía I,Linares J,Feito MJ,Arcos D,Vallet-Regí M,Plotkin LI,Esbrit P,Gortázar AR

    更新日期:2017-12-01 00:00:00

  • Intracellular chloride regulates cell proliferation through the activation of stress-activated protein kinases in MKN28 human gastric cancer cells.

    abstract::Recently, we reported that reduction of intracellular Cl(-) concentration ([Cl(-)](i)) inhibited proliferation of MKN28 gastric cancer cells by diminishing the transition rate from G(1) to S cell-cycle phase through upregulation of p21, cyclin-dependent kinase inhibitor, in a p53-independent manner. However, it is sti...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Ohsawa R,Miyazaki H,Niisato N,Shiozaki A,Iwasaki Y,Otsuji E,Marunaka Y

    更新日期:2010-06-01 00:00:00

  • Role of lipoproteins in growth of human adult arterial endothelial and smooth muscle cells in low lipoprotein-deficient serum.

    abstract::Recently improved culture conditions for human adult arterial endothelial and smooth muscle cells from a wide variety of donors have been used to study the effects of lipoproteins on proliferation of both cell types in low serum culture medium. Optimal growth of endothelial and smooth muscle cells in an optimal nutrie...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Chen JK,Hoshi H,McClure DB,McKeehan WL

    更新日期:1986-11-01 00:00:00

  • Insulin-like growth factor binding protein (IGFBP)-5 is upregulated during both differentiation and apoptosis in primary cultures of mouse mammary epithelial cells.

    abstract::We have previously demonstrated that insulin-like growth factor binding protein-5 (IGFBP-5) is upregulated following treatment of the mouse mammary epithelial cell line HC11 with lactogenic hormones (dexamethasone, insulin, and prolactin-DIP). In addition, we have also shown that IGFBP-5 is upregulated in mammary epit...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Lochrie JD,Phillips K,Tonner E,Flint DJ,Allan GJ,Price NC,Beattie J

    更新日期:2006-05-01 00:00:00

  • Methylation of CLEC14A is associated with its expression and lung adenocarcinoma progression.

    abstract::Our main objective is probing the effect of methylation of CLEC14A on its expression and lung adenocarcinoma (LUAD) progression. Microarray analysis was utilized to screen out differentially downregulated genes with hypermethylation in LUAD tissues. The CLEC14A expression level was measured by western blot analysis an...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Su C,Shi K,Cheng X,Han Y,Li Y,Yu D,Liu Z

    更新日期:2019-03-01 00:00:00

  • Transforming activity of mutant human p53 alleles.

    abstract::Mutant forms of the p53 gene have been shown to cooperate with an activated ras gene in transforming primary cells in culture. The aberrant proteins encoded by p53 mutants are thought to act in a dominant negative manner in these assays. In vivo data, however, reveal that where p53 has undergone genetic change in tumo...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Slingerland JM,Benchimol S

    更新日期:1991-09-01 00:00:00

  • Production of Macrophage Inhibitory Factor (MIF) by Primary Sertoli Cells; Its Possible Involvement in Migration of Spermatogonial Cells.

    abstract::Macrophage migration inhibitory factor (MIF) is a multifunctional molecule. MIF was originally identified as a T-cell-derived factor responsible for the inhibition of macrophage migration. In testicular tissue of adult rats, MIF is constitutively expressed by Leydig cells under physiological conditions. The aim of thi...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Huleihel M,Abofoul-Azab M,Abarbanel Y,Einav I,Levitas E,Lunenfeld E

    更新日期:2017-10-01 00:00:00

  • Identification of the potential key genes for adipogenesis from human mesenchymal stem cells by RNA-Seq.

    abstract::Adipogenesis, a physiological process initiated with the committed preadipocytes expressing adipocyte-specific genes and terminated in mature, differentiated and functional adipocytes, mainly involved with energy homeostasis. Abnormal distribution-changes and dysfunctions in adipogenesis may lead to complex physiopath...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Yi X,Wu P,Liu J,Gong Y,Xu X,Li W

    更新日期:2019-11-01 00:00:00

  • Metabolism and regulation of gene expression by 4-oxoretinol versus all-trans retinoic acid in normal human mammary epithelial cells.

    abstract::We previously demonstrated that 4-oxoretinol (4-oxo-ROL) activated retinoic acid receptors (RARs) in F9 stem cells. We showed that 4-oxo-ROL inhibited the proliferation of normal human mammary epithelial cells (HMECs). To understand the mechanisms by which 4-oxo-ROL regulates HMEC growth we examined gene expression pr...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Liu L,Derguini F,Gudas LJ

    更新日期:2009-09-01 00:00:00

  • Acute L-glutamine deprivation compromises VEGF-a upregulation in A549/8 human carcinoma cells.

    abstract::Tumor ischemia participates in angiogenesis and cancer progression through cellular responses to hypoxia and nutrient deprivation. However, the contribution of amino acids limitation to this process remains poorly understood. Using serum-free cell culture conditions, we tested the impact of L-glutamine deprivation on ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Drogat B,Bouchecareilh M,North S,Petibois C,Déléris G,Chevet E,Bikfalvi A,Moenner M

    更新日期:2007-08-01 00:00:00

  • Effect of epidermal growth factor on rat pleural mesothelial cell growth.

    abstract::We recently reported that the growth of normal rat pleural mesothelial cells (RPMCs) is inhibited by conditioned media from either in vivo or in vitro transformed RPMCs. In this study we report that the growth of normal RPMCs is inhibited by epidermal growth factor (EGF). This was demonstrated by using three methods o...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Van der Meeren A,Levy F,Renier A,Katz A,Jaurand MC

    更新日期:1990-07-01 00:00:00

  • Growth-dependent AIB and meAIB uptake in LLC-PK1 cells: effects of differentiation inducers and of TPA.

    abstract::Cultured pig kidney cells designated LLC-PK1, previously shown to acquire Na+-dependent concentrative transport of hexoses as the cells become growth arrested, also show Na+-dependent concentrative uptake of the amino acid analogs alpha-aminoisobutyric acid (AIB) and (methyl) meAIB. This A system-like transport is mos...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Amsler K,Shaffer C,Cook JS

    更新日期:1983-02-01 00:00:00

  • Epithelial macrophages secrete a deactivating factor for superoxide release.

    abstract::The release of superoxide anion (O2-) by inflammatory macrophages, multinucleated giant cells, and epithelioid cells, obtained by the insertion of round glass coverslips into the subcutaneous tissue of mice, was investigated. O2- was shown to be spontaneously released by cells on the surface of glass coverslips implan...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Camarero VC,Junqueira VB,Colepicolo P,Karnovsky ML,Mariano M

    更新日期:1990-12-01 00:00:00

  • Profiling of retinoid mediated gene expression in synchronized human SCC cells using Atlas human cDNA expression arrays.

    abstract::While retinoids have been demonstrated to inhibit growth of many tumor cells, including SCC cells, the molecular mechanism by which retinoids suppress growth has not been elucidated. We previously found that the growth of SCC cells was significantly inhibited by all-trans-retinoic acid (all-trans-RA) treatment, and th...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Le Q,Soprano DR,Soprano KJ

    更新日期:2002-03-01 00:00:00

  • Unexpected regulation pattern of the IKKβ/NF-κB/MuRF1 pathway with remarkable muscle plasticity in the Daurian ground squirrel (Spermophilus dauricus).

    abstract::As a typical hibernator, the Daurian ground squirrel (Spermophilus dauricus) spends considerable time in a state of reduced activity with prolonged fasting. Despite this, they experience little muscle atrophy and have thus become an interesting anti-disuse muscle atrophy model. The IKKβ/NF-κB signaling pathway is sign...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Wei Y,Gong L,Fu W,Xu S,Wang Z,Zhang J,Ning E,Chang H,Wang H,Gao Y

    更新日期:2018-11-01 00:00:00

  • Thermotolerance induced at a mild temperature of 40 degrees C protects cells against heat shock-induced apoptosis.

    abstract::Apoptosis constitutes a response of organisms to various physiological or pathological stimuli, and to different stresses. The ability of thermotolerance induced at a mild temperature of 40 degrees C to protect against activation of the apoptotic cascade by heat shock was investigated. When Chinese hamster ovary and h...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Bettaieb A,Averill-Bates DA

    更新日期:2005-10-01 00:00:00

  • Can Cholesterol Metabolism Modulation Affect Brain Function and Behavior?

    abstract::Cholesterol is an important component for cell physiology. It regulates the fluidity of cell membranes and determines the physical and biochemical properties of proteins. In the central nervous system, cholesterol controls synapse formation and function and supports the saltatory conduction of action potential. In rec...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Cartocci V,Servadio M,Trezza V,Pallottini V

    更新日期:2017-02-01 00:00:00

  • Role of epimorphin in bile duct formation of rat liver epithelial stem-like cells: involvement of small G protein RhoA and C/EBPβ.

    abstract::Epimorphin/syntaxin 2 is a high conserved and very abundant protein involved in epithelial morphogenesis in various organs. We have shown recently that epimorphin (EPM), a protein exclusively expressed on the surface of hepatic stellate cells and myofibroblasts of the liver, induces bile duct formation of hepatic stem...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Jia Y,Yao H,Zhou J,Chen L,Zeng Q,Yuan H,Shi L,Nan X,Wang Y,Yue W,Pei X

    更新日期:2011-11-01 00:00:00

  • Production of growth factors by type 5 adenovirus transformed rat embryo cells.

    abstract::Transformation of Sprague-Dawley rat embryo (RE) cells and a cloned Fischer rat embryo cell line (CREF) with wild-type (Ad5) or a temperature-sensitive DNA-minus mutant (H5ts125) of type 5 adenovirus results in a reduction in binding of epidermal growth factor (EGF) to cell surface receptors. A reduction in EGF bindin...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Fisher PB,Boersig MR,Graham GM,Weinstein IB

    更新日期:1983-03-01 00:00:00

  • VEGF inhibition alters neurotrophin signalling pathways and induces caspase-3 activation and autophagy in rabbit retina.

    abstract::This study sought to evaluate the prospective role exerted by vascular endothelial growth factor (VEGF) in the modulation of nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) signalling pathways in the rabbit retina. To reach this aim, the anti-VEGF agents aflibercept and ranibizumab were used as ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Segatto M,Fico E,Gharbiya M,Rosso P,Carito V,Tirassa P,Plateroti R,Lambiase A

    更新日期:2019-08-01 00:00:00

  • Human bronchial epithelial cells synthesize cholesterol sulfate during squamous differentiation in vitro.

    abstract::Epithelial cells of the airways can, under pathological conditions, undergo squamous metaplasia. The accumulation of cholesterol sulfate has recently been described as a new marker for squamous cell differentiation in rabbit tracheal epithelial cells. We now report that normal human bronchial epithelial cells in cultu...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Rearick JI,Hesterberg TW,Jetten AM

    更新日期:1987-12-01 00:00:00

  • Critical choices for modeling breast cancer in transgenic mouse models.

    abstract::Modeling breast cancer in the mouse has helped to better define the heterogeneity of human breast cancer. In the recent past, it has become evident that some limitations have restricted the potential benefits that can be achieved with this approach. In this review, we highlight some key points that should be taken int...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Caligiuri I,Rizzolio F,Boffo S,Giordano A,Toffoli G

    更新日期:2012-08-01 00:00:00

  • Cholera toxin and analogues of cyclic AMP stimulate the growth of cultured human mammary epithelial cells.

    abstract::The growth of human epithelial cells is stimulated by cholera toxin and analogues of cyclic AMP, while the growth of breast derived fibroblasts is inhibited. These compounds have little effect on DNA synthesis in the absence of other mitogens but show a synergistic effect with serum and/or EGF. The results suggest tha...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Taylor-Papadimitriou J,Purkis P,Fentiman IS

    更新日期:1980-03-01 00:00:00

  • Biosynthesis of proteoglycans by proliferating and differentiating normal human keratinocytes cultured in serum-free medium.

    abstract::Normal human keratinocytes (NHK) were cultured in serum-free medium, containing low (0.1 mM) or high (2 mM) calcium, to obtain proliferating and differentiating cultures, respectively. Proteoglycan (PG) synthesis of proliferating and differentiating NHK was investigated. Cultures were labeled with 35S-sulfate, and the...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Rahemtulla F,Moorer CM,Wille JJ Jr

    更新日期:1989-07-01 00:00:00

  • Mechanical stimulation of skeletal muscle generates lipid-related second messengers by phospholipase activation.

    abstract::Repetitive mechanical stimulation of cultured avian skeletal muscle increases the synthesis of prostaglandins (PG) E2 and F2 alpha which regulate protein turnover rates and muscle cell growth. These stretch-induced PG increases are reduced in low extracellular calcium medium and by specific phospholipase inhibitors. M...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Vandenburgh HH,Shansky J,Karlisch P,Solerssi RL

    更新日期:1993-04-01 00:00:00

  • Properties of growth hormone receptors in relation to the adipose conversion of 3T3 cells.

    abstract::Cultured preadipose 3T3 cells undergo a process of differentiation in which they convert to adipose cells. Growth hormone promotes this conversion. Since 3T3 sublines vary in their susceptibility to adipose conversion, it was of interest to examine the properties of the growth hormone receptors in relation to that sus...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Nixon T,Green H

    更新日期:1983-06-01 00:00:00

  • An Overview on Predictive Biomarkers of Testicular Germ Cell Tumors.

    abstract::Testicular germ cell tumors (TGCTs) are frequent solid malignant tumors and cause of death in men between 20-40 years of age. Genetic and environmental factors play an important role in the origin and development of TGCTs. Although the majority of TGCTs are responsive to chemotherapy, about 20% of patient presents inc...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Chieffi P

    更新日期:2017-02-01 00:00:00

  • Role of protein kinase C in interleukin 1, anti-T3, and mitogenic lectin-induced interleukin 2 secretion.

    abstract::Activation of T-lymphocytes by antigen, mitogenic lectins, or antibodies against the T-cell receptor complex, particularly in the presence of IL1, induces the secretion of the T-cell growth factor IL2. IL2 then has a major role in regulating the duration and magnitude of the immune response. Interaction of antigen, an...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Mills GB,May C,Hill M,Gelfand EW

    更新日期:1989-11-01 00:00:00

  • Smart materials as scaffolds for tissue engineering.

    abstract::In this review, we focused our attention on the more important natural extracellular matrix (ECM) molecules (collagen and fibrin), employed as cellular scaffolds for tissue engineering and on a class of semi-synthetic materials made from the fusion of specific oligopeptide sequences, showing biological activities, wit...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Rosso F,Marino G,Giordano A,Barbarisi M,Parmeggiani D,Barbarisi A

    更新日期:2005-06-01 00:00:00