Abstract:
:Human striated muscle samples, from male control and Duchenne muscular dystrophy-affected children, were subjected to cluster-time-of-flight secondary ion mass spectrometry (cluster-ToF-SIMS) imaging using a 25 keV Bi(3)(+) liquid metal ion gun under static SIMS conditions. Spectra and ion density maps, or secondary ion images, were acquired in both positive and negative ion mode over several areas of 500 x 500 microm(2) (image resolution, 256 x 256 pixels). Characteristic distributions of various lipids were observed. Vitamin E and phosphatidylinositols were found to concentrate within the cells, whereas intact phosphocholines accumulated over the most damaged areas of the dystrophic muscles, together with cholesterol and sphingomyelin species. Fatty acyl chain composition varied depending on the region, allowing estimation of the local damage extent.
journal_name
J Lipid Resjournal_title
Journal of lipid researchauthors
Tahallah N,Brunelle A,De La Porte S,Laprévote Odoi
10.1194/jlr.M700421-JLR200subject
Has Abstractpub_date
2008-02-01 00:00:00pages
438-54issue
2eissn
0022-2275issn
1539-7262pii
M700421-JLR200journal_volume
49pub_type
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