Lipid compositional analysis of pulmonary surfactant monolayers and monolayer-associated reservoirs.

abstract：:The hypothesis of lipid domains in cellular plasma membranes is well established. However, direct visualization of the domains has been difficult. Here we report direct visualization of plasma membrane cholesterol microdomains modulated by agents that affect cholesterol trafficking to and from the plasma membrane. The...

journal_title：Journal of lipid research

authors： Kruth HS,Ifrim I,Chang J,Addadi L,Perl-Treves D,Zhang WY

更新日期：2001-09-01 00:00:00

abstract：:The skin surface represents our interface with the external environment, and as such, is our first line of defense against microbial colonization and infection. Lipids at the skin surface are thought to underlie at least part of an antimicrobial barrier. Some of these lipids are synthesized in the epidermis and are ca...

journal_title：Journal of lipid research

authors： Drake DR,Brogden KA,Dawson DV,Wertz PW

更新日期：2008-01-01 00:00:00

abstract：:Isotopic measurement of biosynthetic rates of lipids in VLDL particles has long posed difficult technical problems. In this review, key methodologic issues and recent technical advances are discussed. A common problem for all biosynthetic measurements is the requirement to measure isotopic labeling of the true intrace...

journal_title：Journal of lipid research

authors： Parks EJ,Hellerstein MK

更新日期：2006-08-01 00:00:00

abstract：:Trans-10,cis-12 conjugated linoleic acid (CLA) has previously been shown to be the CLA isomer responsible for CLA-induced reductions in body fat in animal models, and we have shown that this isomer, but not the cis-9,trans-11 CLA isomer, specifically decreased triglyceride (TG) accumulation in primary human adipocytes...

journal_title：Journal of lipid research

authors： Brown JM,Boysen MS,Jensen SS,Morrison RF,Storkson J,Lea-Currie R,Pariza M,Mandrup S,McIntosh MK

更新日期：2003-07-01 00:00:00

abstract：:Over the past several years, considerable progress has been made in the development of gene therapy as a therapeutic strategy for a variety of inherited metabolic diseases, including neuropathic lysosomal storage disorders (LSDs). The premise of gene therapy for this group of diseases is borne of findings that genetic...

journal_title：Journal of lipid research

authors： Cheng SH

更新日期：2014-09-01 00:00:00

abstract：:Three glycosides of 13-hydroxydocosanoic acid isolated from Candida bogoriensis were characterized by quantitating the amount of carbohydrate, acetate, and hydroxy acid in each, and by gas-liquid chromatography and mass spectrometry of their methyl ester, trimethylsilyl ether derivatives. One of the glycosides was the...

journal_title：Journal of lipid research

authors： Esders TW,Light RJ

更新日期：1972-09-01 00:00:00

abstract：:Apolipoproteins, such as apolipoprotein A-I (apoA-I), can stimulate cholesterol efflux from cells expressing the ATP binding cassette transporter A1 (ABCA1). The nature of the molecular interaction between these cholesterol acceptors and ABCA1 is controversial, and models suggesting a direct protein-protein interactio...

journal_title：Journal of lipid research

authors： Fitzgerald ML,Morris AL,Chroni A,Mendez AJ,Zannis VI,Freeman MW

更新日期：2004-02-01 00:00:00

abstract：:During intestinal absorption amino acids must traverse the lipid-rich epithelial cell membrane, possibly in a lipid-soluble form. In a search for such a form, we have determined the ability of lipid extracted from intestinal mucosa to bind valine. After incubation in a valine-containing medium this lipid (defined as t...

journal_title：Journal of lipid research

authors： Reiser S,Christiansen PA

更新日期：1968-09-01 00:00:00

abstract：:ABCA12 mutations disrupt the skin barrier and cause harlequin ichthyosis. We previously showed Abca12(-/-) skin has increased glucosylceramide (GlcCer) and correspondingly lower amounts of ceramide (Cer). To examine why loss of ABCA12 leads to accumulation of GlcCer, de novo sphingolipid synthesis was assayed using [(...

journal_title：Journal of lipid research

authors： Haller JF,Cavallaro P,Hernandez NJ,Dolat L,Soscia SJ,Welti R,Grabowski GA,Fitzgerald ML,Freeman MW

更新日期：2014-03-01 00:00:00

abstract：:Hepatic free cholesterol levels are influenced by cholesterol synthesis and ester formation, which, in turn, might regulate cholesterol secretion into bile and plasma. We manipulated the rates of hepatic cholesterol synthesis and esterification and measured biliary and very low density lipoprotein (VLDL) cholesterol s...

journal_title：Journal of lipid research

authors： Stone BG,Evans CD

更新日期：1992-11-01 00:00:00

abstract：:Chiral phase high performance liquid chromatographic resolution of sn-1,2(2,3)- and X-1,3-diacylglycerols generated by partial Grignard degradation from natural triacylglycerols was carried out using a chiral column (25 cm x 4.6 mm i.d.) containing (R)-(+)-1-(1-napthyl)ethylamine polymer chemically bonded to 300A wide...

journal_title：Journal of lipid research

authors： Itabashi Y,Kukis A,Marai L,Takagi T

更新日期：1990-09-01 00:00:00

abstract：:Oxidative stress promotes acute kidney injury (AKI). Higher concentrations of HDL cholesterol are associated with less AKI. To test the hypothesis that HDL antioxidant activity is associated with AKI after cardiac surgery, we quantified HDL particle size and number, paraoxonase-1 activity, and isofuran concentrations ...

journal_title：Journal of lipid research

authors： Smith LE,Smith DK,Yancey PG,Kon V,Remaley AT,Billings FT 4th,Linton MF

更新日期：2021-01-13 00:00:00

abstract：:Tissue-specific regulation of LPL has been widely studied in rats. Previous studies reported that in vivo administration of adrenaline and acute stress cause an increase in plasma LPL activity coinciding with a decrease in white adipose tissue (WAT) LPL activity. We studied the speed of LPL activity changes during 30 ...

journal_title：Journal of lipid research

authors： Casanovas A,Parramon N,de la Cruz F,Andrés O,Terencio J,López-Tejero MD,Llobera M

更新日期：2007-04-01 00:00:00

abstract：:Lymph chylomicrons of different sizes are known to be cleared at different rates, but the underlying mechanism for this effect has not been resolved. To investigate the differences in clearance rates between small and large particles, chylomicron-like lipid emulsions labeled with radioactive triolein and cholesteryl o...

journal_title：Journal of lipid research

authors： Martins IJ,Mortimer BC,Miller J,Redgrave TG

更新日期：1996-12-01 00:00:00

abstract：:The relationship between overnight postabsorptive (fasting) respiratory exchange ratio (RER) and plasma FFA concentrations was addressed using data from three separate protocols, each of which involved careful control of the antecedent diet. Protocol 1 examined the relationship between fasting RER and the previous day...

journal_title：Journal of lipid research

authors： Jensen MD,Bajnárek J,Lee SY,Nielsen S,Koutsari C

更新日期：2009-09-01 00:00:00

abstract：:The hepatic synthesis of sterol was measured in the male Mongolian gerbil (Meriones unguiculatus) in vivo following the administration of [3H]water by monitoring the incorporation of radioactivity into digitonin-precipitable sterol. A diurnal rhythm in cholesterol synthesis was exhibited under conditions of ad libitum...

journal_title：Journal of lipid research

authors： Mercer NJ,Holub BJ

更新日期：1981-07-01 00:00:00

abstract：:13C nuclear magnetic resonance (NMR) spectra were obtained at 50.3 and 100.5 MHz for methanolic and aqueous mixtures of sodium taurocholate, 1-monocapryloyl-rac-glycerol, and caprylic acid. Distortionless Enhancement by Polarization Transfer (DEPT) was used to improve spectral sensitivity and resolution, and to genera...

journal_title：Journal of lipid research

authors： Wang D,Hadipour NL,Jerlin EA,Stark RE

更新日期：1992-03-01 00:00:00

abstract：:Lymph fistula rats with either biliary fistula or pancreaticobiliary fistula were used to measure coenzyme A-independent mucosal cholesterol esterifying activity in the presence and absence of pancreatic exocrine secretion. Efficiency of pancreatic diversion was verified to minimize contamination of mucosal homogenate...

journal_title：Journal of lipid research

authors： Watt SM,Simmonds WJ

更新日期：1981-01-01 00:00:00

abstract：:Binding of the medium-chain fatty acids (MCFA), octanoic (OCT) and decanoic (DEC) acid, to human serum albumin (HSA) has been studied by 13C NMR spectroscopy. NMR spectra at 35 degrees C showed an apparently homogeneous binding environment (a single, narrow resonance for the 13C-enriched carboxyl carbon) at different ...

journal_title：Journal of lipid research

authors： Kenyon MA,Hamilton JA

更新日期：1994-03-01 00:00:00

abstract：:Mature endothelial lipase (EL) is a 68 kDa glycoprotein. In HepG2 cells infected with adenovirus encoding human EL, the mature EL was detectable in the cell lysates and heparin-releasable fractions. In contrast, cell media of these cells contained two EL fragments: an N-terminal 40 kDa fragment and a C-terminal 28 kDa...

journal_title：Journal of lipid research

authors： Gauster M,Hrzenjak A,Schick K,Frank S

更新日期：2005-05-01 00:00:00

abstract：:We investigated the role of caveolae in uptake and intracellular trafficking of long chain fatty acids (LCFA) in HepG2 human hepatoma cells. The uptake of [(3)H]oleic acid and [(3)H]stearic acid into HepG2 cells was measured by radioactive assays and internalization of the non-metabolizable fluorescent fatty acid 12-(...

journal_title：Journal of lipid research

authors： Pohl J,Ring A,Stremmel W

更新日期：2002-09-01 00:00:00

abstract：:The palmitate (PA) binding and transport capacity of human and bovine red cell membranes enables us to establish, in a biological system, the existence of a well-defined monomer concentration in equilibrium with PA bound to bovine serum albumin (BSA, 30 microM) inside the resealed red cell ghosts. Supernatants of susp...

journal_title：Journal of lipid research

authors： Bojesen IN,Bojesen E

更新日期：1992-09-01 00:00:00

abstract：:Four hours after intraportal injection of retinoic acid-(14)C into bile duct-cannulated rats, less than 10% of the radioactivity was recovered in the liver, intestine, and kidneys. Within 6 hr, 40% of the radioactivity had appeared in bile. When suspensions of retinol-(14)C or retinal were similarly injected, 25-35% o...

journal_title：Journal of lipid research

authors： Zachman RD,Dunagin PE Jr,Olson JA

更新日期：1966-01-01 00:00:00

abstract：:The CYP27A gene encodes a mitochondrial cytochrome P450 enzyme, sterol 27-hydroxylase, that is expressed in many different tissues and plays an important role in cholesterol and bile acid metabolism. In humans, CYP27A deficiency leads to cerebrotendinous xanthomatosis. To gain insight into the roles of CYP27A in the r...

journal_title：Journal of lipid research

authors： Dubrac S,Lear SR,Ananthanarayanan M,Balasubramaniyan N,Bollineni J,Shefer S,Hyogo H,Cohen DE,Blanche PJ,Krauss RM,Batta AK,Salen G,Suchy FJ,Maeda N,Erickson SK

更新日期：2005-01-01 00:00:00

abstract：:This study was undertaken to determine the mechanisms that regulate cholesterol synthesis in vivo and to quantitate the relative importance of the liver and extrahepatic tissues as sites for sterol synthesis. Rats were administered [3H]water intravenously and killed 1 hour later. The amount of [3H]water incorporated i...

journal_title：Journal of lipid research

authors： Jeske DJ,Dietschy JM

更新日期：1980-03-01 00:00:00

abstract：:Steroids are primarily present in human fluids in their sulfated forms. Profiling of these compounds is important from both diagnostic and physiological points of view. Here, we present a novel method for the quantification of 11 intact steroid sulfates in human serum by LC-MS/MS. The compounds analyzed in our method,...

journal_title：Journal of lipid research

authors： Sánchez-Guijo A,Oji V,Hartmann MF,Traupe H,Wudy SA

更新日期：2015-09-01 00:00:00

abstract：:Genome-wide association (GWA) studies represent a powerful strategy for identifying susceptibility genes for complex diseases in human populations but results must be confirmed and replicated. Because of the close homology between mouse and human genomes, the mouse can be used to add evidence to genes suggested by hum...

journal_title：Journal of lipid research

authors： Leduc MS,Lyons M,Darvishi K,Walsh K,Sheehan S,Amend S,Cox A,Orho-Melander M,Kathiresan S,Paigen B,Korstanje R

更新日期：2011-06-01 00:00:00

abstract：:Adipocyte triglyceride storage provides a reservoir of energy that allows the organism to survive times of nutrient scarcity, but excessive adiposity has emerged as a health problem in many areas of the world. Monoacylglycerol acyltransferase (MGAT) acylates monoacylglycerol to produce diacylglycerol; the penultimate ...

journal_title：Journal of lipid research

authors： Liss KHH,Lutkewitte AJ,Pietka T,Finck BN,Franczyk M,Yoshino J,Klein S,Hall AM

更新日期：2018-09-01 00:00:00

abstract：:The de novo ceramide synthesis pathway is essential to human biology and health, but genetic influences remain unexplored. The core function of this pathway is the generation of biologically active ceramide from its precursor, dihydroceramide. Dihydroceramides have diverse, often protective, biological roles; converse...

journal_title：Journal of lipid research

authors： Blackburn NB,Michael LF,Meikle PJ,Peralta JM,Mosior M,McAhren S,Bui HH,Bellinger MA,Giles C,Kumar S,Leandro AC,Almeida M,Weir JM,Mahaney MC,Dyer TD,Almasy L,VandeBerg JL,Williams-Blangero S,Glahn DC,Duggirala R,Ko

更新日期：2019-09-01 00:00:00

abstract：:This review presents an overview of mammalian phospholipid synthesis and the cellular locations of the biochemical activities that produce membrane lipid molecular species. The generalized endoplasmic reticulum compartment is a central site for membrane lipid biogenesis, and examples of the emerging relationships betw...

journal_title：Journal of lipid research

authors： Fagone P,Jackowski S

更新日期：2009-04-01 00:00:00