Abstract:
:This article reports the development of an optical imaging technique, confocal light absorption and scattering spectroscopic (CLASS) microscopy, capable of noninvasively determining the dimensions and other physical properties of single subcellular organelles. CLASS microscopy combines the principles of light-scattering spectroscopy (LSS) with confocal microscopy. LSS is an optical technique that relates the spectroscopic properties of light elastically scattered by small particles to their size, refractive index, and shape. The multispectral nature of LSS enables it to measure internal cell structures much smaller than the diffraction limit without damaging the cell or requiring exogenous markers, which could affect cell function. Scanning the confocal volume across the sample creates an image. CLASS microscopy approaches the accuracy of electron microscopy but is nondestructive and does not require the contrast agents common to optical microscopy. It provides unique capabilities to study functions of viable cells, which are beyond the capabilities of other techniques.
journal_name
Proc Natl Acad Sci U S Aauthors
Itzkan I,Qiu L,Fang H,Zaman MM,Vitkin E,Ghiran IC,Salahuddin S,Modell M,Andersson C,Kimerer LM,Cipolloni PB,Lim KH,Freedman SD,Bigio I,Sachs BP,Hanlon EB,Perelman LTdoi
10.1073/pnas.0708669104subject
Has Abstractpub_date
2007-10-30 00:00:00pages
17255-60issue
44eissn
0027-8424issn
1091-6490pii
0708669104journal_volume
104pub_type
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