Abstract:
:Neurones in the lumbosacral spinal cord are known to play a significant role in ejaculation. In these same areas neurones containing nitric oxide synthase (NOS) have been described. This raised the question as to whether there is a physiological role for nitrous oxide (NO) in the spinal cord in sexual behavior. We first established immunohistochemical localization of NOS positive neurones in the lumbosacral spinal cord. NOS positive neurones were found in several areas of the lumbosacral cord. Namely the intermediolateral column (IML) at L(1)-L(4) and sacral cord; the dorsal gray commissure above the central canal at L(1)-L(2); the ventral gray area of lamina X below the central canal at L(3)-L(4); the superficial laminae of the dorsal horn at all levels. Secondly, we examined the role of NO in the generation of synchronized bursting activity within the vas deferens nerve and associated penile muscles, typical of sexual responses in the male anesthetized rat. NO modulators were applied directly to the spinal cord at T(13)-L(4) via a catheter placed subdurally (intrathecal) and their effect on the genital responses evoked by systemic administration of p-chloroamphetamine (PCA) or apomorphine (apo) (both 1 mg/kg) was observed. All responses evoked by PCA (n=4) or apo (n=3) were abolished or reduced (n=1) during intrathecal NOS inhibition using N((G)) nitro-L-Arginine methyl ester (l-NAME, 200 mM, 20-microl). NOS inhibition using l-NAME was reversed with simultaneous intrathecal application of the NO substrate l-arginine (100 mM, 20-microl, n=3). The selective neuronal NOS inhibitor 1-(2-trifluoro-methylphenyl) imidazole (100 mM, 20-microl, TRIM) also abolished all responses evoked by PCA (n=3). There was evidence that the responses within the vas deferens nerve (VDN) after PCA or apo were enhanced following NO donation using sodium nitroprusside (SNP, 1 mM, 20-microl). Furthermore, a PCA-like response within the VDN was evoked following intrathecally applied l-glutamic acid (200 mM, 20-microl) in six of 26 animals and also by intrathecal SNP in two of eight animals. In conclusion the results suggest a significant excitatory role for NO in the bursting pattern of synchronized discharge generated in autonomic and somatic outflows from the lumbosacral cord by neurones governing ejaculation.
journal_name
Neurosciencejournal_title
Neuroscienceauthors
Brack KE,Watkins N,Pyner S,Coote JHdoi
10.1016/j.neuroscience.2007.09.010subject
Has Abstractpub_date
2007-12-05 00:00:00pages
487-97issue
2eissn
0306-4522issn
1873-7544pii
S0306-4522(07)01112-8journal_volume
150pub_type
杂志文章相关文献
NEUROSCIENCE文献大全abstract:OBJECTIVE:Women are more prone to major depressive disorders (MDDs) and the incidence of MDD in women is almost twice that of men. Insular cortex abnormalities are a common finding in neuroanatomical studies of patients with MDD. However, it remains largely unclear whether female MDD patients at different clinical stag...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2013.12.058
更新日期:2014-03-14 00:00:00
abstract::The distribution of the C-terminal glycopeptide of the vasopressin prohormone was mapped in rat brain by an immunocytochemical method using antibodies to the sheep glycopeptide. The antibodies did not react with vasopressin, oxytocin or their related neurophysins. Stained neural perikarya were observed in the hypothal...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(87)90046-7
更新日期:1987-06-01 00:00:00
abstract::Numerous studies have shown that human endogenous retrovirus W family (HERV-W) envelope gene (env) is related to various diseases but the underlying mechanism has remained poorly understood. Our previous study showed that there was abnormal expression of HERV-W env in sera of patients with schizophrenia. In this paper...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2013.05.033
更新日期:2013-09-05 00:00:00
abstract::The posterior parahippocampal gyrus (PPHG) of the non-human primate brain has a distinct dual role in cortical neural systems. On the one hand, it is a critical link in providing the entorhinal cortex and hippocampal formation with cortical input, while on the other hand it receives output from these structures and pr...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2008.03.077
更新日期:2008-06-23 00:00:00
abstract::An increased frequency of apolipoprotein E E4 allele has been reported in patients with late onset Alzheimer's disease. Apolipoprotein E participates in the transport of cholesterol and other lipids and interferes with the growth and regeneration of both peripheral and central nervous system tissues during development...
journal_title:Neuroscience
pub_type: 临床试验,杂志文章
doi:10.1016/0306-4522(95)00014-a
更新日期:1995-07-01 00:00:00
abstract::Lamina I of the spinal dorsal horn contains a diverse mixture of neurons. Among these, a group of giant neurons (Waldeyer cells) has long been recognized. In this study we have used immunocytochemistry to characterize a population of Waldeyer cells which were identified by the presence of high levels of the glycine re...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(00)00445-0
更新日期:2001-01-01 00:00:00
abstract::The pathophysiologic basis of tardive dyskinesia remains unclear. It has been proposed that tardive dyskinesia may be a result of excitotoxic neurodegeneration in the striatum caused by a neuroleptic-induced increase in striatal glutamate release and impaired energy metabolism. To investigate this hypothesis, haloperi...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(98)00160-2
更新日期:1998-12-01 00:00:00
abstract::The vanilloid receptor subtype 1 (VR1) is expressed in a sub-population of small dorsal root ganglion (DRG) neurones in mammals and serves as the common transducer of the pain-producing signals, such as noxious heat, acids and capsaicin [Caterina et al., Nature 389 (1997) 816-824; Tominaga et al., Neuron 21 (1998) 531...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(02)00051-9
更新日期:2002-01-01 00:00:00
abstract::Excitotoxic mechanisms involving alpha-amino-3-hydroxy-5-methyl-isoxazole-4-propionate (AMPA)/kainate receptors play an important role in mediating cellular damage in spinal cord injury. However, the precise cellular mechanisms of glutamate release from non-synaptic white matter are not well understood. We examined ho...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(01)00385-2
更新日期:2001-01-01 00:00:00
abstract::The effect of intrathecal or intracerebroventricular administration of the GABAB receptor agonist, baclofen, on rhythmic contractions induced by distension of the urinary bladder (micturition reflex) was evaluated in urethane-anesthetized rats. Baclofen inhibited bladder motility acting at central nervous system sites...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(92)90350-b
更新日期:1992-01-01 00:00:00
abstract::Drinking alcohol in moderation is often considered a health-conscious behavior, associated with improved cardiovascular and brain health. However, "moderate" amounts of alcohol include drinking 3-4 alcohol beverages in a day, which is closer to binge drinking and may do more harm than good. Here we examined how daily ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2012.08.018
更新日期:2012-11-08 00:00:00
abstract::The ability to generate subthreshold membrane potential oscillations in neurons from the inferior olive nucleus has been attributed to the electrical properties of these neurons, as well as to the properties of the network. In the present in vitro study we quantitatively characterized both intrinsic membrane and netwo...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(96)00588-x
更新日期:1997-05-01 00:00:00
abstract::This study shows that the neurons of the entopeduncular nucleus are derived from a longitudinal slab of isochronically generated neurons on day 11 and 15 h of gestation. Many neurons of this longitudinal slab which we have named the basal forebrain cell column, originate from an ependymal matrix closely associated wit...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(86)90032-1
更新日期:1986-03-01 00:00:00
abstract::Differences in the uptake of tracers from radioactive 2-deoxyglucose ([1,2-3H] and [2,6-3H]), and glucose ([1-3H], [3-3H]) into hippocampal regions were investigated by freeze-mount microautoradiography after 45 min for 2-deoxyglucose, and after 15 and 45 min for glucose. Silver grains were assessed quantitatively by ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(89)90378-3
更新日期:1989-01-01 00:00:00
abstract::Acidic fibroblast growth factor (aFGF) is a neurotrophic factor which is a powerful neuroprotective and neuroregenerative factor of the nervous system. Prior study had shown that levels of FGFs significantly increase following ischemic injury, reflecting a physiological protection mechanism. However, few reports demon...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2015.07.074
更新日期:2015-10-01 00:00:00
abstract::Melatonin, an indoleamine hormone secreted into circulation at night primarily by the brain's pineal gland, has been shown to have a wide variety of actions on the development and physiology of neurons in the CNS. Acting via two G-protein-coupled membrane receptors (MT1 and MT2), melatonin modulates neurogenesis, syna...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2014.07.018
更新日期:2014-09-26 00:00:00
abstract::GABA is the main inhibitory neurotransmitter of the CNS. It is well established that activation of GABA(A) receptors favors sleep. Three generations of hypnotics are based on these GABA(A) receptor-mediated inhibitory processes. The first and second generation of hypnotics (barbiturates and benzodiazepines respectivel...
journal_title:Neuroscience
pub_type: 杂志文章,评审
doi:10.1016/s0306-4522(02)00034-9
更新日期:2002-01-01 00:00:00
abstract::This study investigated amphetamine-induced striatal dopamine release after intraventricular unilateral fetal mesencephalic grafts in otherwise intact rats. Dopamine was monitored in vivo by differential pulse voltammetry. In grafted animals, amphetamine-induced dopamine release was decreased compared to sham-grafted,...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(97)00236-4
更新日期:1997-12-01 00:00:00
abstract::Dopaminergic mechanisms were analyzed in an isolated preparation of the turtle olfactory bulb. Field potentials were evoked by antidromic or orthodromic stimulation, and the effects determined of pharmacological manipulations of the bathing medium. In the presence of dopamine or a dopamine agonist, apomorphine, there ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(83)90005-2
更新日期:1983-04-01 00:00:00
abstract::Acute and chronic exposure to psychostimulants results in altered function of G-protein-coupled receptors in the forebrain. It is believed that neuroadaptations in G-protein signaling contribute to behavioral sensitivity to psychostimulants that persists over a prolonged drug-free period. Proteins termed activators of...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2010.03.013
更新日期:2010-06-16 00:00:00
abstract::Golgi, Nissl, Bielschowsky and cholinesterase techniques have been used to analyze the optic tectum of the weakly electric teleost fish Eigenmannia virescens and Apteronotus leptorhynchus. Six layers are readily distinguished: a fairly thick stratum marginale, a narrow stratum opticum and stratum fibrosum et griseum s...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(86)90190-9
更新日期:1986-05-01 00:00:00
abstract::Estrogen is likely involved in the gender specific differences in coping with stress. Activation of catecholamine (CA) biosynthetic enzyme gene expression in central and peripheral CA systems plays a key role in response to stress and in regulation of the cardiovascular system. Here we examined whether estradiol can m...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2004.12.040
更新日期:2005-01-01 00:00:00
abstract::Local cerebral glucose utilization was assessed during whisker stimulation by 2-deoxyglucose autoradiography. Whisker stimulation increased local cerebral glucose utilization in brainstem, thalamus and whisker sensory cortex in normal rats. Whereas whisker stimulation increased glucose metabolism in brainstem, whisker...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(94)90300-x
更新日期:1994-04-01 00:00:00
abstract::Thrombin plays important roles in the pathology of intracerebral hemorrhage (ICH). The recruitment of activated microglia, accompanied by thrombin-induced phosphorylation of the mitogen-activated protein kinase (MAPK) family, contributes to ICH-associated neuron loss. Here we investigated the possibility that sesamin,...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2012.11.057
更新日期:2013-03-01 00:00:00
abstract::High-intensity sound can induce seizures in susceptible animals. After repeated acoustic stimuli changes in behavioural seizure repertoire and epileptic EEG activity might be seen in recruited limbic and forebrain structures, a phenomenon known as audiogenic kindling. It is postulated that audiogenic kindling can prod...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2015.09.040
更新日期:2015-12-03 00:00:00
abstract::We have raised antisera against the GABA analogues gamma-vinyl GABA, diaminobutyric acid and gabaculine. These analogues are thought to be substrates for high-affinity GABA transporters. Retinae were exposed to micromolar concentrations of these analogues in the presence or absence of uptake inhibitors and then fixed ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(96)00097-8
更新日期:1996-08-01 00:00:00
abstract::Creatine supplementation has been shown to protect neurons from oxidative damage due to its antioxidant and ergogenic functions. These features have led to the hypothesis of creatine supplementation use during pregnancy as prophylactic treatment to prevent CNS damage, such as hypoxic-ischemic encephalopathy. Unfortuna...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2015.11.017
更新日期:2016-01-15 00:00:00
abstract::The nodose ganglion contains placode-derived visceral sensory neurons of the vagus nerve. Previous study showed that axotomy-induced deafferentation reduced the number of tyrosine hydroxylase-immunoreactive and increased the number of vasoactive intestinal peptide-immunoreactive neurons in the ganglion. The present st...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(94)90555-x
更新日期:1994-11-01 00:00:00
abstract::A newly developed benzoylpyrrolidine drug (BDP-20) that increases the size of fast, excitatory synaptic responses was examined for its effects on the kinetic properties of alpha-amino-3-hydroxy-5-methyl-4-isoxalepropionic acid (AMPA)-type glutamate receptors. When long pulses of glutamate were applied to excised hippo...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(96)00263-1
更新日期:1996-11-01 00:00:00
abstract::The central part of the retina, which includes the fovea, is substantially overrepresented in the topographic map of the retina in the striate cortex. We tested whether this simply reflects the uneven distribution of ganglion cells in the retina in accordance with the "principle" of peripheral scaling, or whether ther...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(95)00589-7
更新日期:1996-06-01 00:00:00